Gene Details:

Functional Descriptions:

  • RMS encodes a mitochondrial protein, 178 aa in length, of unknown function, unlike the majority of other Rf genes cloned thus far, which encode pentatricopeptide repeat proteins.
  • Through positional cloning, we identified a nuclear candidate gene, RETROGRADE-REGULATED MALE STERILITY (RMS) for Rf17, a fertility restorer gene for Chinese wild rice (CW)-type CMS in rice (Oryza sativa L.
  • RNA interference-mediated gene silencing of RMS restored fertility to a CMS plant, whereas its overexpression in the fertility restorer line induced pollen abortion.
  • We found that a reduced-expression allele of the RMS gene restored fertility in haploid pollen, whereas a normal-expression allele caused pollen to die in the CW-type CMS.
  • The mRNA expression level of RMS in mature anthers depended on cytoplasmic genotype, suggesting that RMS is a candidate gene to be regulated via retrograde signaling.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os04g40020.1
    TTTCTCTCTCTCCCCCCACCTTCCTTCTCTTTCCTTCTTTTTCCCCTTTTCCTTCCTTCCCGTCGACCACGTTGATGCCGACGGTGGCTGACACATGACGCGCGGTGGTGCAGCAACGCGGGGAGGCGGAAGGCGGTGCGGGGAGGCGGCGCTCGCTGAGGTGCTCTCCCTCTCCCTTTGCGGCGACGAGGCGACGGCGGCGGAGCTCGGAGCGGGATGGCGATGGCGGCGGCGGAGCTCGGGGTGGGGATGGCAAAGGCGGCGGCTCTCCACAACGACGACTTGGCGAGGCGACGACGACGGCGAGGAAGATGCAGCCACGACAGCACATGGGCAGGGATGGCACTGGAGGCACATGGCGGCCACGGCGACGGAGTCGAGGGAGAGGCGCGGGAAGCCGCAAGGGGTGGCCCTCTGCTCCTCGCCGGCCTTGCCTGGTCCTCCGCCGCCTTGTCGCCGTGGCTCCGCTGGTTCCGGGAGGCAGGGGCGAGAGAGAGGAGGGAGGGAGTGAGGTGGAGCGCGGGGACTCGGTTCCAGGCGATGGTGGAAGGGGGACGGCAGGGGAGGCAGGGCAAAAGAGGCGCTCGCGCCTCCGGTCCATTTCGGCGGAGGGCGGCGATGGAAGCAGCCGAGGCAGCGTTGAAGAGTTTGGGGACAGAGAGAATGCGAGATGTGGGAAGAAAAATATGGTTGTGGGTCCCACAATCCCACAGTTGGAGAGCCAGTTTTGGCTGGCCAAGTTTAGCCACCTAGGGAGCCCCTTTGGCCAACCAAATAGCTTCGAGAGTAGGATAGCCAGTATGTTGGAGCTCGTTTTTTTTTTCAAAATTTCTAAATTTTAGTTTGGGGAGTGAAATA
CDS Sequence
  • >LOC_Os04g40020.1
    ATGGCGATGGCGGCGGCGGAGCTCGGGGTGGGGATGGCAAAGGCGGCGGCTCTCCACAACGACGACTTGGCGAGGCGACGACGACGGCGAGGAAGATGCAGCCACGACAGCACATGGGCAGGGATGGCACTGGAGGCACATGGCGGCCACGGCGACGGAGTCGAGGGAGAGGCGCGGGAAGCCGCAAGGGGTGGCCCTCTGCTCCTCGCCGGCCTTGCCTGGTCCTCCGCCGCCTTGTCGCCGTGGCTCCGCTGGTTCCGGGAGGCAGGGGCGAGAGAGAGGAGGGAGGGAGTGAGGTGGAGCGCGGGGACTCGGTTCCAGGCGATGGTGGAAGGGGGACGGCAGGGGAGGCAGGGCAAAAGAGGCGCTCGCGCCTCCGGTCCATTTCGGCGGAGGGCGGCGATGGAAGCAGCCGAGGCAGCGTTGAAGAGTTTGGGGACAGAGAGAATGCGAGATGTGGGAAGAAAAATATGGTTGTGGGTCCCACAATCCCACAGTTGGAGAGCCAGTTTTGGCTGGCCAAGTTTAGCCACCTAG
Protein Sequence
  • >LOC_Os04g40020.1
    MAMAAAELGVGMAKAAALHNDDLARRRRRRGRCSHDSTWAGMALEAHGGHGDGVEGEAREAARGGPLLLAGLAWSSAALSPWLRWFREAGARERREGVRWSAGTRFQAMVEGGRQGRQGKRGARASGPFRRRAAMEAAEAALKSLGTERMRDVGRKIWLWVPQSHSWRASFGWPSLAT*