Gene Details:
- MSU gene ID: LOC_Os04g40020
- RAPdb gene ID: Os04g0475900
- Gene Symbol: Rf17 RMS
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- RMS encodes a mitochondrial protein, 178 aa in length, of unknown function, unlike the majority of other Rf genes cloned thus far, which encode pentatricopeptide repeat proteins.
- Through positional cloning, we identified a nuclear candidate gene, RETROGRADE-REGULATED MALE STERILITY (RMS) for Rf17, a fertility restorer gene for Chinese wild rice (CW)-type CMS in rice (Oryza sativa L.
- RNA interference-mediated gene silencing of RMS restored fertility to a CMS plant, whereas its overexpression in the fertility restorer line induced pollen abortion.
- We found that a reduced-expression allele of the RMS gene restored fertility in haploid pollen, whereas a normal-expression allele caused pollen to die in the CW-type CMS.
- The mRNA expression level of RMS in mature anthers depended on cytoplasmic genotype, suggesting that RMS is a candidate gene to be regulated via retrograde signaling.
Function-related keywords:
- mitochondria , fertility , pollen , anther , sterility
Literature:
- Suppressed expression of Retrograde-Regulated Male Sterility restores pollen fertility in cytoplasmic male sterile rice plants . DOI: 10.1073/pnas.0901860106 ; PMID: 19458265
Related News:
Gene Resources:
- NCBI ID: AB481199
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os04g40020.1
TTTCTCTCTCTCCCCCCACCTTCCTTCTCTTTCCTTCTTTTTCCCCTTTTCCTTCCTTCCCGTCGACCACGTTGATGCCGACGGTGGCTGACACATGACGCGCGGTGGTGCAGCAACGCGGGGAGGCGGAAGGCGGTGCGGGGAGGCGGCGCTCGCTGAGGTGCTCTCCCTCTCCCTTTGCGGCGACGAGGCGACGGCGGCGGAGCTCGGAGCGGGATGGCGATGGCGGCGGCGGAGCTCGGGGTGGGGATGGCAAAGGCGGCGGCTCTCCACAACGACGACTTGGCGAGGCGACGACGACGGCGAGGAAGATGCAGCCACGACAGCACATGGGCAGGGATGGCACTGGAGGCACATGGCGGCCACGGCGACGGAGTCGAGGGAGAGGCGCGGGAAGCCGCAAGGGGTGGCCCTCTGCTCCTCGCCGGCCTTGCCTGGTCCTCCGCCGCCTTGTCGCCGTGGCTCCGCTGGTTCCGGGAGGCAGGGGCGAGAGAGAGGAGGGAGGGAGTGAGGTGGAGCGCGGGGACTCGGTTCCAGGCGATGGTGGAAGGGGGACGGCAGGGGAGGCAGGGCAAAAGAGGCGCTCGCGCCTCCGGTCCATTTCGGCGGAGGGCGGCGATGGAAGCAGCCGAGGCAGCGTTGAAGAGTTTGGGGACAGAGAGAATGCGAGATGTGGGAAGAAAAATATGGTTGTGGGTCCCACAATCCCACAGTTGGAGAGCCAGTTTTGGCTGGCCAAGTTTAGCCACCTAGGGAGCCCCTTTGGCCAACCAAATAGCTTCGAGAGTAGGATAGCCAGTATGTTGGAGCTCGTTTTTTTTTTCAAAATTTCTAAATTTTAGTTTGGGGAGTGAAATA
CDS Sequence
- >LOC_Os04g40020.1
ATGGCGATGGCGGCGGCGGAGCTCGGGGTGGGGATGGCAAAGGCGGCGGCTCTCCACAACGACGACTTGGCGAGGCGACGACGACGGCGAGGAAGATGCAGCCACGACAGCACATGGGCAGGGATGGCACTGGAGGCACATGGCGGCCACGGCGACGGAGTCGAGGGAGAGGCGCGGGAAGCCGCAAGGGGTGGCCCTCTGCTCCTCGCCGGCCTTGCCTGGTCCTCCGCCGCCTTGTCGCCGTGGCTCCGCTGGTTCCGGGAGGCAGGGGCGAGAGAGAGGAGGGAGGGAGTGAGGTGGAGCGCGGGGACTCGGTTCCAGGCGATGGTGGAAGGGGGACGGCAGGGGAGGCAGGGCAAAAGAGGCGCTCGCGCCTCCGGTCCATTTCGGCGGAGGGCGGCGATGGAAGCAGCCGAGGCAGCGTTGAAGAGTTTGGGGACAGAGAGAATGCGAGATGTGGGAAGAAAAATATGGTTGTGGGTCCCACAATCCCACAGTTGGAGAGCCAGTTTTGGCTGGCCAAGTTTAGCCACCTAG
Protein Sequence
- >LOC_Os04g40020.1
MAMAAAELGVGMAKAAALHNDDLARRRRRRGRCSHDSTWAGMALEAHGGHGDGVEGEAREAARGGPLLLAGLAWSSAALSPWLRWFREAGARERREGVRWSAGTRFQAMVEGGRQGRQGKRGARASGPFRRRAAMEAAEAALKSLGTERMRDVGRKIWLWVPQSHSWRASFGWPSLAT*