Gene Details:

Functional Descriptions:

  • Here, we demonstrate that the dioxygenase for auxin oxidation (DAO) gene, encoding a putative 2-oxoglutarate-dependent-Fe (II) dioxygenase, is essential for anther dehiscence, pollen fertility, and seed initiation in rice.
  • Furthermore, exogenous application of IAA or overexpression of the auxin biosynthesis gene OsYUCCA1 phenocopies the DAO mutants.
  • Collectively, these data support a key role of DAO in auxin catabolism and maintenance of auxin homeostasis central to plant reproductive development.
  • Rice mutant lines lacking a functional DAO display increased levels of free IAA in anthers and ovaries.
  • We show that recombinant DAO converts the active IAA into biologically inactive 2-oxoindole-3-acetic acid (OxIAA) in vitro.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os04g39980.1
    AGAAGCAAACACTGCGATCATCCGAAGCAATCTCAATCTCACCTGTCCATATCGCGCACGAATTCGTAAAGTGCAAGCTAGAAACATGTGTCACATGTCGTGCTGATCGTTCGATCCACCACGGTGCGAGCGGTGAGTGTCGTGACGAAGCAAAGGCAGAGGCAGCAGCGGGAAACGGCGCCCCCCCGTGTGTCGATCGGCTGTCTCGCCGAACAGGGCAAATACGCCAACAAAAAACGGCCTCGTTTTCCCTTGCTCGCACGCACCGTGTCGCTCCCAGGCGACAAGCCGTGGCGCGACTCGCCGCCCCCCTGCGCCCCCTCGCCCCCGCGCGTCGCTTTCACGTCGCACCTCTAAATACCACCACCCCGGACTCGGCGGCGAGCCCCCGCGCCAAGAACGAAAGGCGAGAGTGAGAGAGATGGTGGAGATCCCGGCGATCGACCTGCGGCTGGCCGGCGGCGGCGGCGGCGCGGAGGAGACGGCGAGGCTGCGCGACGCGTGCGCGCGGCTGGGCTGCTTCCGGGTGTCGGGGCACGGCGTGCCGCCGGGGCTCCAGGCCGAGATGAAGGCCGCGGTGCGCGCGCTCTTCGACCTCCCCGACGACGCCAAGCGCCGCAACGCCGACATCATCCCGGGCAGCGGCTACGTCCCGCCCGGCACAGCCAACCCGCTCTACGAGGCCTTCGGCCTCTGCGACGCCGCCGCCCCCGCCGACGTCGACGCCTTCTGCGCCCGCCTCGACGCGCCGCCCCACGTCAGGGAGACCGTGAAGGCGTACGCCGAGAGGATGCACTCGCTGATCGTGGACGTCGCCGGCAAGGTCGCCGCGAGCCTGGGGCTGCACGGCGCCTCGTTCCAGGACTGGCCGTGCCAGTTCCGCATGAACAGGTACAACTACACGCAGGACTCCGTGGGCTCCCCCGGCGTGCAGGTCCACACGGACTCCGGCTTCCTCACCGTGCTCCAGGAGGACGAGTGCGTTGGCGGGCTCGAGGTGCTCGACCCCGCCGCCGGCGAGTTCGTCCCCGTCGACCCCCTCCCCGGCTCGTTCGTCGTCAACGTCGGCGACGTCGGCCAGGCGTGGAGCAACGGGAGGCTGCACAACGTGAAGCACAGGGTGCAGTGCGTGGCGGCGGTGCCGCGCGTGTCGATCGCCATGTTCCTGCTGGCGCCCAAGGACGACACGGTGAGCGCGCCGGGGGAGCTGGTGGACGGCGAGCACCCGCGCCGGTACAGGGAGTTCAAGTACGACGACTACCGGAGGCTCCGGCTGTCCACCGGCGAGCGCGCCGGCGAGGCGCTCGCGCGTCTGGCGGCCTGACACCTGGCCACGGTCCATTGTCGCGACCGTTTATTACGCGGAGTGCTCACTGCTGACTAGAATGTCCATTAGGCCTCCCTGTATGGCCGTGTCGATTCTGAACAATAAAATCTTCGACGTACGACAGTGAAAAAAATTTGTAAAGGATGCTGAGGGGATGGGAATTTTAGGGGTTAATAAGTCTTTTGTTTGGCTGGAGGAGATGATGAGTTGTAGGAATAACTGGAAGGTGGAGTTTGTGAGTAAATTCCCACCTTTTTAATATAGAGGACTATAATTGGGAGGAAAGTCCCTTTCTGAGCTCTCTAATTTTAGTTAGTTGAATCTTACACGTCCACCATCACGTAAAATCTAATTTCCATCACCATAATTCTTATCTTTGTCAGTATTTTTTTTCTTTTGAAATACAATACGTGTACACTCACCCCTATTAACACGCACACCCTATAGAACAATGATAGTAAATATTTCCAATTATTAA
CDS Sequence
  • >LOC_Os04g39980.1
    ATGGTGGAGATCCCGGCGATCGACCTGCGGCTGGCCGGCGGCGGCGGCGGCGCGGAGGAGACGGCGAGGCTGCGCGACGCGTGCGCGCGGCTGGGCTGCTTCCGGGTGTCGGGGCACGGCGTGCCGCCGGGGCTCCAGGCCGAGATGAAGGCCGCGGTGCGCGCGCTCTTCGACCTCCCCGACGACGCCAAGCGCCGCAACGCCGACATCATCCCGGGCAGCGGCTACGTCCCGCCCGGCACAGCCAACCCGCTCTACGAGGCCTTCGGCCTCTGCGACGCCGCCGCCCCCGCCGACGTCGACGCCTTCTGCGCCCGCCTCGACGCGCCGCCCCACGTCAGGGAGACCGTGAAGGCGTACGCCGAGAGGATGCACTCGCTGATCGTGGACGTCGCCGGCAAGGTCGCCGCGAGCCTGGGGCTGCACGGCGCCTCGTTCCAGGACTGGCCGTGCCAGTTCCGCATGAACAGGTACAACTACACGCAGGACTCCGTGGGCTCCCCCGGCGTGCAGGTCCACACGGACTCCGGCTTCCTCACCGTGCTCCAGGAGGACGAGTGCGTTGGCGGGCTCGAGGTGCTCGACCCCGCCGCCGGCGAGTTCGTCCCCGTCGACCCCCTCCCCGGCTCGTTCGTCGTCAACGTCGGCGACGTCGGCCAGGCGTGGAGCAACGGGAGGCTGCACAACGTGAAGCACAGGGTGCAGTGCGTGGCGGCGGTGCCGCGCGTGTCGATCGCCATGTTCCTGCTGGCGCCCAAGGACGACACGGTGAGCGCGCCGGGGGAGCTGGTGGACGGCGAGCACCCGCGCCGGTACAGGGAGTTCAAGTACGACGACTACCGGAGGCTCCGGCTGTCCACCGGCGAGCGCGCCGGCGAGGCGCTCGCGCGTCTGGCGGCCTGA
Protein Sequence
  • >LOC_Os04g39980.1
    MVEIPAIDLRLAGGGGGAEETARLRDACARLGCFRVSGHGVPPGLQAEMKAAVRALFDLPDDAKRRNADIIPGSGYVPPGTANPLYEAFGLCDAAAPADVDAFCARLDAPPHVRETVKAYAERMHSLIVDVAGKVAASLGLHGASFQDWPCQFRMNRYNYTQDSVGSPGVQVHTDSGFLTVLQEDECVGGLEVLDPAAGEFVPVDPLPGSFVVNVGDVGQAWSNGRLHNVKHRVQCVAAVPRVSIAMFLLAPKDDTVSAPGELVDGEHPRRYREFKYDDYRRLRLSTGERAGEALARLAA*