Information report for Os4BGlu12
Gene Details
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Functional Descriptions
- Os4BGlu12 expressed in recombinant Escherichia coli hydrolyzed β-(1,3;1,4)-glucooligosaccharides generated by the wounding-induced rice endo-(1,3;1,4)-β-glucanase OsEGL1, suggesting that both enzymes may act in concert in remodeling of damaged cell wall.
- The amino acid residues predicted to line the active site of Os4BGlu12 are more similar to those of cyanogenic and flavonoid β-glucosidases than oligosaccharide hydrolases, and it may function in defense, as well as in cell wall-derived oligosaccharide break-down.
- Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides.
- Rice Os4BGlu12 β-glucosidase is a family 1 glycoside hydrolase, the transcript levels of which have previously been found to be induced in response to herbivore attack and salinity stress.
- Here, high levels of Os4BGlu12 transcripts were also detected in the shoot during germination, in the leaf sheath and stem of mature rice plants under normal growth conditions.
- The cDNA of the Os4BGlu12 gene, which encodes a protein identical at 40 of 44 amino acid residues with the N-terminal sequence of a cell wall-bound enzyme previously purified from germinating rice, was isolated by RT-PCR from rice seedlings.
- To begin this analysis, Os4BGlu12 beta-glucosidase was characterized and found to have high exoglucanase activity, consistent with a role in cell wall metabolism.
- Native Os4BGlu12 and its complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside (DNP2FG) were crystallized using 19% polyethylene glycol (3350 or 2000, respectively) in 0.
Functional Keywords
Literature and News
- The crystal structure of rice (Oryza sativa L.) Os4BGlu12, an oligosaccharide and tuberonic acid glucoside-hydrolyzing β-glucosidase with significant thioglucohydrolase activity . DOI: 10.1016/j.abb.2011.04.005 ; PMID: 21521631
- Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides . DOI: 10.1016/j.plantsci.2010.05.013
- Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase . DOI: 10.1186/1471-2229-6-33 ; PMID: 17196101
- Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase . DOI: 10.1107/S174430911000103X ; PMID: 20208171
Gene Resources
- NCBI ID: AK062776
Sequences
cDNA Sequence
- >LOC_Os04g39880.2
ACAGCCATCTCTCTATTTTTCCTTTTTCTGTCCATGGCGGCAGCAGGGGCAATGCCCGGTGGCCTTCTCCTCACGTTCCTCCTCCTTGCTGTCGTCGCTTCCGGCGCCTACAATGGCGCCGGCGAGCCGCCGGTCAGCCGGAGAAGCTTCCCCAAGGGGTTCATCTTCGGGACAGCCTCGTCGTCGTATCAGTACGAGGGTGGCGCAGCGGAGGGCGGCAGAGGACCAAGCATCTGGGACACCTTCACACATCAGCACCCAGAGAAAATCGCCGACAGAAGCAACGGGGATGTGGCTTCGGATTCCTACCATCTCTACAAGGAAGATGTGCGCCTCATGAAGGATATGGGAATGGATGCATACAGGTTCTCCATCTCATGGACAAGAATCCTTCCAAATGGAAGTCTGAGGGGTGGAGTCAACAAAGAAGGCATAAAGTACTACAATAATTTGATCAATGAGCTATTATCGAAAGGGGTGCAACCGTTTATTACCCTTTTCCACTGGGACTCACCTCAGGCGTTGGAAGATAAATATAATGGATTTCTTAGCCCTAATATCATAAATGACTTTAAGGACTATGCTGAAATCTGCTTCAAGGAGTTTGGTGACAGAGTGAAAAATTGGATCACCTTCAATGAGCCTTGGACTTTCTGCTCTAATGGCTATGCAACTGGCCTGTTTGCACCAGGCCGTTGTTCGCCCTGGGAGAAGGGAAATTGCAGTGTTGGAGATTCAGGAAGGGAGCCTTACACTGCATGCCATCATCAACTACTTGCCCACGCGGAAACTGTTCGGTTGTACAAAGCGAAATATCAGGCCTTACAAAAAGGGAAGATTGGAATAACTCTGGTCTCGCACTGGTTTGTTCCCTTCTCCCGCTCCAAATCCAACGATGATGCTGCAAAGCGTGCTATAGACTTCATGTTTGGATGGTTTATGGATCCCCTCATTAGAGGCGACTACCCCCTAAGCATGAGAGGATTGGTTGGGAATCGCTTGCCACAGTTCACTAAAGAACAGTCTAAGTTGGTCAAGGGTGCATTTGACTTTATTGGACTTAACTACTACACTGCGAACTATGCTGATAACCTTCCTCCATCAAATGGCCTTAACAACAGCTATACCACCGATTCTCGAGCTAATCTTACCGGTATGTAGGCTGCTTCACCTTGGCTTTACGTCTACCCTCAAGGCTTCCGTGACTTGCTACTTTATGTTAAGGAGAACTATGGCAATCCTACCGTCTACATCACTGAAAATGGCGTTGATGAATTCAACAATAAGACCTTACCACTCCAAGAAGCCTTGAAGGATGACGCTAGGATAGAATACTACCACAAGCACCTCCTTTCCCTGCTAAGTGCTATAAGGGATGGAGCAAACGTGAAGGGATACTTTGCATGGTCGCTGCTTGATAACTTCGAGTGGTCGAACGGCTACACAGTTCGATTTGGGATAAACTTCGTAGATTACAATGACGGAAGGAAGAGATACCCCAAGAACTCTGCCCATTGGTTCAAGAAGTTCCTCCTGAAATGATAAAAAAGCACCATTGCACTCATTGCTTTTCTCATTGAATAATAAAAGAGTATATGTTTTGCGTGAATTTGGTACAAGAGATGGAAGTAATCCAGTTAAATAATAAATCCCTCTCTTACTGCATGTCTGCATCTATATCTTAACTTCCTGCACATAGTTTAGCAAAAGGTATATAGTTACACACCTTCTATCAACACA - >LOC_Os04g39880.3
ACAGCCATCTCTCTATTTTTCCTTTTTCTGTCCATGGCGGCAGCAGGGGCAATGCCCGGTGGCCTTCTCCTCACGTTCCTCCTCCTTGCTGTCGTCGCTTCCGGCGCCTACAATGGCGCCGGCGAGCCGCCGGTCAGCCGGAGAAGCTTCCCCAAGGGGTTCATCTTCGGGACAGCCTCGTCGTCGTATCAGTACGAGGGTGGCGCAGCGGAGGGCGGCAGAGGACCAAGCATCTGGGACACCTTCACACATCAGCACCCAGAGAAAATCGCCGACAGAAGCAACGGGGATGTGGCTTCGGATTCCTACCATCTCTACAAGGAAGATGTGCGCCTCATGAAGGATATGGGAATGGATGCATACAGGTTCTCCATCTCATGGACAAGAATCCTTCCAAATGGAAGTCTGAGGGGTGGAGTCAACAAAGAAGGCATAAAGTACTACAATAATTTGATCAATGAGCTATTATCGAAAGGGGTGCAACCGTTTATTACCCTTTTCCACTGGGACTCACCTCAGGCGTTGGAAGATAAATATAATGGATTTCTTAGCCCTAATATCATAAATGACTTTAAGGACTATGCTGAAATCTGCTTCAAGGAGTTTGGTGACAGAGTGAAAAATTGGATCACCTTCAATGAGCCTTGGACTTTCTGCTCTAATGGCTATGCAACTGGCCTGTTTGCACCAGGCCGTTGTTCGCCCTGGGAGAAGGGAAATTGCAGTGTTGGAGATTCAGGAAGGGAGCCTTACACTGCATGCCATCATCAACTACTTGCCCACGCGGAAACTGTTCGGTTGTACAAAGCGAAATATCAGGCCTTACAAAAAGGGAAGATTGGAATAACTCTGGTCTCGCACTGGTTTGTTCCCTTCTCCCGCTCCAAATCCAACGATGATGCTGCAAAGCGTGCTATAGACTTCATGTTTGGATGGTTTATGGATCCCCTCATTAGAGGCGACTACCCCCTAAGCATGAGAGGATTGGTTGGGAATCGCTTGCCACAGTTCACTAAAGAACAGTCTAAGTTGGTCAAGGGTGCATTTGACTTTATTGGACTTAACTACTACACTGCGAACTATGCTGATAACCTTCCTCCATCAAATGGCCTTAACAACAGCTATACCACCGATTCTCGAGCTAATCTTACCGGTATGTAGGCTGCTTCACCTTGGCTTTACGTCTACCCTCAAGGCTTCCGTGACTTGCTACTTTATGTTAAGGAGAACTATGGCAATCCTACCGTCTACATCACTGAAAATGGCGTTGATGAATTCAACAATAAGACCTTACCACTCCAAGAAGCCTTGAAGGATGACGCTAGGATAGAATACTACCACAAGCACCTCCTTTCCCTGCTAAGTGCTATAAGGGATGGAGCAAACGTGAAGGGATACTTTGCATGGTCGCTGCTTGATAACTTCGAGTGGTCGAACGGCTACACAGTTCGATTTGGGATAAACTTCGTAGATTACAATGACGGAAGGAAGAGATACCCCAAGAACTCTGCCCATTGGTTCAAGAAGTTCCTCCTGAAATGATAAAAAAGCACCATTGCACTCATTGCTTTTCTCATTGAATAATAAAAGAGTATATGTTTTGCGTGAATTTGGTACAAGAGATGGAAGTAATCCAGTTAAATAATAAATCCCTCTCTTACTGCATGTCTGCATCTATATCTTAACTTCCTGCACATAGTTTAGCAAAAGGTATATAGTTACACACCTTCTATCAACACA - >LOC_Os04g39880.1
ACAGCCATCTCTCTATTTTTCCTTTTTCTGTCCATGGCGGCAGCAGGGGCAATGCCCGGTGGCCTTCTCCTCACGTTCCTCCTCCTTGCTGTCGTCGCTTCCGGCGCCTACAATGGCGCCGGCGAGCCGCCGGTCAGCCGGAGAAGCTTCCCCAAGGGGTTCATCTTCGGGACAGCCTCGTCGTCGTATCAGTACGAGGGTGGCGCAGCGGAGGGCGGCAGAGGACCAAGCATCTGGGACACCTTCACACATCAGCACCCAGAGAAAATCGCCGACAGAAGCAACGGGGATGTGGCTTCGGATTCCTACCATCTCTACAAGGAAGATGTGCGCCTCATGAAGGATATGGGAATGGATGCATACAGGTTCTCCATCTCATGGACAAGAATCCTTCCAAATGGAAGTCTGAGGGGTGGAGTCAACAAAGAAGGCATAAAGTACTACAATAATTTGATCAATGAGCTATTATCGAAAGGGGTGCAACCGTTTATTACCCTTTTCCACTGGGACTCACCTCAGGCGTTGGAAGATAAATATAATGGATTTCTTAGCCCTAATATCATAAATGACTTTAAGGACTATGCTGAAATCTGCTTCAAGGAGTTTGGTGACAGAGTGAAAAATTGGATCACCTTCAATGAGCCTTGGACTTTCTGCTCTAATGGCTATGCAACTGGCCTGTTTGCACCAGGCCGTTGTTCGCCCTGGGAGAAGGGAAATTGCAGTGTTGGAGATTCAGGAAGGGAGCCTTACACTGCATGCCATCATCAACTACTTGCCCACGCGGAAACTGTTCGGTTGTACAAAGCGAAATATCAGGCCTTACAAAAAGGGAAGATTGGAATAACTCTGGTCTCGCACTGGTTTGTTCCCTTCTCCCGCTCCAAATCCAACGATGATGCTGCAAAGCGTGCTATAGACTTCATGTTTGGATGGTTTATGGATCCCCTCATTAGAGGCGACTACCCCCTAAGCATGAGAGGATTGGTTGGGAATCGCTTGCCACAGTTCACTAAAGAACAGTCTAAGTTGGTCAAGGGTGCATTTGACTTTATTGGACTTAACTACTACACTGCGAACTATGCTGATAACCTTCCTCCATCAAATGGCCTTAACAACAGCTATACCACCGATTCTCGAGCTAATCTTACCGGTATGTAGGCTGCTTCACCTTGGCTTTACGTCTACCCTCAAGGCTTCCGTGACTTGCTACTTTATGTTAAGGAGAACTATGGCAATCCTACCGTCTACATCACTGAAAATGGCGTTGATGAATTCAACAATAAGACCTTACCACTCCAAGAAGCCTTGAAGGATGACGCTAGGATAGAATACTACCACAAGCACCTCCTTTCCCTGCTAAGTGCTATAAGGGATGGAGCAAACGTGAAGGGATACTTTGCATGGTCGCTGCTTGATAACTTCGAGTGGTCGAACGGCTACACAGTTCGATTTGGGATAAACTTCGTAGATTACAATGACGGAAGGAAGAGATACCCCAAGAACTCTGCCCATTGGTTCAAGAAGTTCCTCCTGAAATGATAAAAAAGCACCATTGCACTCATTGCTTTTCTCATTGAATAATAAAAGAGTATATGTTTTGCGTGAATTTGGTACAAGAGATGGAAGTAATCCAGTTAAATAATAAATCCCTCTCTTACTGCATGTCTGCATCTATATCTTAACTTCCTGCACATAGTTTAGCAAAAGGTATATAGTTACACACCTTCTATCAACACA
CDS Sequence
- >LOC_Os04g39880.2
ATGGCGGCAGCAGGGGCAATGCCCGGTGGCCTTCTCCTCACGTTCCTCCTCCTTGCTGTCGTCGCTTCCGGCGCCTACAATGGCGCCGGCGAGCCGCCGGTCAGCCGGAGAAGCTTCCCCAAGGGGTTCATCTTCGGGACAGCCTCGTCGTCGTATCAGTACGAGGGTGGCGCAGCGGAGGGCGGCAGAGGACCAAGCATCTGGGACACCTTCACACATCAGCACCCAGAGAAAATCGCCGACAGAAGCAACGGGGATGTGGCTTCGGATTCCTACCATCTCTACAAGGAAGATGTGCGCCTCATGAAGGATATGGGAATGGATGCATACAGGTTCTCCATCTCATGGACAAGAATCCTTCCAAATGGAAGTCTGAGGGGTGGAGTCAACAAAGAAGGCATAAAGTACTACAATAATTTGATCAATGAGCTATTATCGAAAGGGGTGCAACCGTTTATTACCCTTTTCCACTGGGACTCACCTCAGGCGTTGGAAGATAAATATAATGGATTTCTTAGCCCTAATATCATAAATGACTTTAAGGACTATGCTGAAATCTGCTTCAAGGAGTTTGGTGACAGAGTGAAAAATTGGATCACCTTCAATGAGCCTTGGACTTTCTGCTCTAATGGCTATGCAACTGGCCTGTTTGCACCAGGCCGTTGTTCGCCCTGGGAGAAGGGAAATTGCAGTGTTGGAGATTCAGGAAGGGAGCCTTACACTGCATGCCATCATCAACTACTTGCCCACGCGGAAACTGTTCGGTTGTACAAAGCGAAATATCAGGCCTTACAAAAAGGGAAGATTGGAATAACTCTGGTCTCGCACTGGTTTGTTCCCTTCTCCCGCTCCAAATCCAACGATGATGCTGCAAAGCGTGCTATAGACTTCATGTTTGGATGGTTTATGGATCCCCTCATTAGAGGCGACTACCCCCTAAGCATGAGAGGATTGGTTGGGAATCGCTTGCCACAGTTCACTAAAGAACAGTCTAAGTTGGTCAAGGGTGCATTTGACTTTATTGGACTTAACTACTACACTGCGAACTATGCTGATAACCTTCCTCCATCAAATGGCCTTAACAACAGCTATACCACCGATTCTCGAGCTAATCTTACCGGTATGTAG - >LOC_Os04g39880.3
ATGGCGGCAGCAGGGGCAATGCCCGGTGGCCTTCTCCTCACGTTCCTCCTCCTTGCTGTCGTCGCTTCCGGCGCCTACAATGGCGCCGGCGAGCCGCCGGTCAGCCGGAGAAGCTTCCCCAAGGGGTTCATCTTCGGGACAGCCTCGTCGTCGTATCAGTACGAGGGTGGCGCAGCGGAGGGCGGCAGAGGACCAAGCATCTGGGACACCTTCACACATCAGCACCCAGAGAAAATCGCCGACAGAAGCAACGGGGATGTGGCTTCGGATTCCTACCATCTCTACAAGGAAGATGTGCGCCTCATGAAGGATATGGGAATGGATGCATACAGGTTCTCCATCTCATGGACAAGAATCCTTCCAAATGGAAGTCTGAGGGGTGGAGTCAACAAAGAAGGCATAAAGTACTACAATAATTTGATCAATGAGCTATTATCGAAAGGGGTGCAACCGTTTATTACCCTTTTCCACTGGGACTCACCTCAGGCGTTGGAAGATAAATATAATGGATTTCTTAGCCCTAATATCATAAATGACTTTAAGGACTATGCTGAAATCTGCTTCAAGGAGTTTGGTGACAGAGTGAAAAATTGGATCACCTTCAATGAGCCTTGGACTTTCTGCTCTAATGGCTATGCAACTGGCCTGTTTGCACCAGGCCGTTGTTCGCCCTGGGAGAAGGGAAATTGCAGTGTTGGAGATTCAGGAAGGGAGCCTTACACTGCATGCCATCATCAACTACTTGCCCACGCGGAAACTGTTCGGTTGTACAAAGCGAAATATCAGGCCTTACAAAAAGGGAAGATTGGAATAACTCTGGTCTCGCACTGGTTTGTTCCCTTCTCCCGCTCCAAATCCAACGATGATGCTGCAAAGCGTGCTATAGACTTCATGTTTGGATGGTTTATGGATCCCCTCATTAGAGGCGACTACCCCCTAAGCATGAGAGGATTGGTTGGGAATCGCTTGCCACAGTTCACTAAAGAACAGTCTAAGTTGGTCAAGGGTGCATTTGACTTTATTGGACTTAACTACTACACTGCGAACTATGCTGATAACCTTCCTCCATCAAATGGCCTTAACAACAGCTATACCACCGATTCTCGAGCTAATCTTACCGGTATGTAG - >LOC_Os04g39880.1
ATGGCGGCAGCAGGGGCAATGCCCGGTGGCCTTCTCCTCACGTTCCTCCTCCTTGCTGTCGTCGCTTCCGGCGCCTACAATGGCGCCGGCGAGCCGCCGGTCAGCCGGAGAAGCTTCCCCAAGGGGTTCATCTTCGGGACAGCCTCGTCGTCGTATCAGTACGAGGGTGGCGCAGCGGAGGGCGGCAGAGGACCAAGCATCTGGGACACCTTCACACATCAGCACCCAGAGAAAATCGCCGACAGAAGCAACGGGGATGTGGCTTCGGATTCCTACCATCTCTACAAGGAAGATGTGCGCCTCATGAAGGATATGGGAATGGATGCATACAGGTTCTCCATCTCATGGACAAGAATCCTTCCAAATGGAAGTCTGAGGGGTGGAGTCAACAAAGAAGGCATAAAGTACTACAATAATTTGATCAATGAGCTATTATCGAAAGGGGTGCAACCGTTTATTACCCTTTTCCACTGGGACTCACCTCAGGCGTTGGAAGATAAATATAATGGATTTCTTAGCCCTAATATCATAAATGACTTTAAGGACTATGCTGAAATCTGCTTCAAGGAGTTTGGTGACAGAGTGAAAAATTGGATCACCTTCAATGAGCCTTGGACTTTCTGCTCTAATGGCTATGCAACTGGCCTGTTTGCACCAGGCCGTTGTTCGCCCTGGGAGAAGGGAAATTGCAGTGTTGGAGATTCAGGAAGGGAGCCTTACACTGCATGCCATCATCAACTACTTGCCCACGCGGAAACTGTTCGGTTGTACAAAGCGAAATATCAGGCCTTACAAAAAGGGAAGATTGGAATAACTCTGGTCTCGCACTGGTTTGTTCCCTTCTCCCGCTCCAAATCCAACGATGATGCTGCAAAGCGTGCTATAGACTTCATGTTTGGATGGTTTATGGATCCCCTCATTAGAGGCGACTACCCCCTAAGCATGAGAGGATTGGTTGGGAATCGCTTGCCACAGTTCACTAAAGAACAGTCTAAGTTGGTCAAGGGTGCATTTGACTTTATTGGACTTAACTACTACACTGCGAACTATGCTGATAACCTTCCTCCATCAAATGGCCTTAACAACAGCTATACCACCGATTCTCGAGCTAATCTTACCGGTATGTAG
Protein Sequence
- >LOC_Os04g39880.2
MAAAGAMPGGLLLTFLLLAVVASGAYNGAGEPPVSRRSFPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANLTGM* - >LOC_Os04g39880.3
MAAAGAMPGGLLLTFLLLAVVASGAYNGAGEPPVSRRSFPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANLTGM* - >LOC_Os04g39880.1
MAAAGAMPGGLLLTFLLLAVVASGAYNGAGEPPVSRRSFPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANLTGM*