Gene Details:

• MSU gene ID: LOC_Os04g39880
• RAPdb gene ID: Os04g0474800
• Gene Symbol: Os4BGlu12
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Os4BGlu12 expressed in recombinant Escherichia coli hydrolyzed β-(1,3;1,4)-glucooligosaccharides generated by the wounding-induced rice endo-(1,3;1,4)-β-glucanase OsEGL1, suggesting that both enzymes may act in concert in remodeling of damaged cell wall.
• The amino acid residues predicted to line the active site of Os4BGlu12 are more similar to those of cyanogenic and flavonoid β-glucosidases than oligosaccharide hydrolases, and it may function in defense, as well as in cell wall-derived oligosaccharide break-down.
• Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides.
• Rice Os4BGlu12 β-glucosidase is a family 1 glycoside hydrolase, the transcript levels of which have previously been found to be induced in response to herbivore attack and salinity stress.
• Here, high levels of Os4BGlu12 transcripts were also detected in the shoot during germination, in the leaf sheath and stem of mature rice plants under normal growth conditions.
• The cDNA of the Os4BGlu12 gene, which encodes a protein identical at 40 of 44 amino acid residues with the N-terminal sequence of a cell wall-bound enzyme previously purified from germinating rice, was isolated by RT-PCR from rice seedlings.
• To begin this analysis, Os4BGlu12 beta-glucosidase was characterized and found to have high exoglucanase activity, consistent with a role in cell wall metabolism.
• Native Os4BGlu12 and its complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside (DNP2FG) were crystallized using 19% polyethylene glycol (3350 or 2000, respectively) in 0.

Function-related keywords:

• cell-wall ,  salinity ,  shoot ,  growth ,  leaf ,  seedling ,  stem ,  sheath ,  ethylene ,  salinity-stress ,  defense

Literature:

• The crystal structure of rice (Oryza sativa L.) Os4BGlu12, an oligosaccharide and tuberonic acid glucoside-hydrolyzing β-glucosidase with significant thioglucohydrolase activity .  DOI:  10.1016/j.abb.2011.04.005 ;  PMID:  21521631
• Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell wall-β-glucan-derived oligosaccharides and glycosides .  DOI:  10.1016/j.plantsci.2010.05.013
• Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase .  DOI:  10.1186/1471-2229-6-33 ;  PMID:  17196101
• Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase .  DOI:  10.1107/S174430911000103X ;  PMID:  20208171

Related News:

Gene Resources:

• NCBI ID: AK062776
• UniProt accessions:

Sequences: