Gene Details:

Functional Descriptions:

  • OsMYB80 regulates anther development and pollen fertility by targeting multiple biological pathways.
  • To understand how OsMYB80 regulates anther development, RNA-seq analysis was conducted to identify genes differentially regulated by OsMYB80 in rice anthers.
  • The OsMYB80 mutants exhibited premature tapetum cell death, lack of Ubisch bodies, no exine, and microspore degeneration.
  • Here we isolated two allelic male sterile mutants of OsMYB80 and investigated how OsMYB80 regulates male fertility in rice.
  • Functional annotation of the 188 genes suggested that OsMYB80 regulates male fertility by directly targeting multiple biological processes.
  • Moreover, BM1 affects the expression of several known genes related to tapetum and microspore development.
  • Collectively, our results suggest that BM1 is one of key regulators for rice male fertility and may serve as a potential target for rice male-sterile line breeding and hybrid seed production.
  • The BM1 mutant exhibited slightly lagging meiosis, aborted transition of the tapetum to a secretory type, premature tapetal degeneration, and abnormal pollen exine formation, leading to ultimately lacks of visible pollens in the mature white anthers.
  • Map-based cloning, complementation and targeted mutagenesis using CRISPR/Cas9 technology demonstrated that the mutated LOC_Os04g39470 is the causal gene in BM1.
  • Results: In this study, we obtained several lines in which the MYB transcription factor OsMS188 was knocked out through the CRISPR-Cas9 approach.
  • OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice.
  • The OsMS188 lines exhibited a male-sterile phenotype with aberrant development and degeneration of tapetal cells, absence of the sexine layer and defective anther cuticles.
  • Conclusion: Overall, OsMS188 is a key regulator of tapetum development and pollen wall formation.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os04g39470.1
    ATTTATAACACGCGGCGCGGCCGTGTGAGCGCCGCACGCACGCAGCAGCAGCGGAATCTCGACGGCGGCGACATCATGGGGCGGGTGCCGTGCTGCGAGAAGGACAACGTGAAGCGCGGGCAGTGGACGCCCGAGGAGGACAACAAGCTGCTCTCCTACATCACCCAGTACGGCACCCGCAACTGGCGCCTCATCCCCAAGAACGCCGGGTTGCAGCGGTGCGGGAAGAGCTGCCGGCTGCGGTGGACCAACTACCTCCGGCCCGACCTCAAGCACGGCGAGTTCACCGACGCCGAGGAGCAGACCATCATCAAGCTCCACTCCGTCGTCGGCAACAGGTGGTCGGTGATCGCGGCGCAGCTTCCGGGGCGGACGGACAACGACGTGAAGAACCACTGGAACACGAAGCTGAAGAAGAAGCTGTCCGGGATGGGCATCGACCCCGTCACGCACAAGTCCTTCTCGCACCTCATGGCCGAGATCGCCACCACGCTGGCGCCGCCGCAGGTGGCGCACCTCGCCGAGGCCGCGCTGGGGTGCTTCAAGGACGAGATGCTCCACCTCCTCACCAAGAAGCGCCCCTCCGACTTCCCCTCGCCCGCCGTGCACGACGGCGCCGGCGCCGGCGCCAGCGCGTCCGCGCTCGCCGCGCCCTGTTTCCCCGCCGCGCCGCCGCACCACCCGCAGGCCGACGACACCATCGAGCGCATCAAGCTCGGCCTGTCCCGCGCCATCATGAGCGATCCCTCCACCGCCTCCGCCGCCGCCGCCGCCGCCGCGCCCTCCGCCCCCGCGGAGGACAAGCCGTGGCCGCCCGGCGACATGTCCGAGGGGCTCGCCGGGATGTACGCCACGTACAACCCGGCGGCGCACGCGCACGCGCAGGCCCAGGCCGAGTTCCGGTACGACGGGGCCTCCGCGGCGCAGGGCTACGTCCTCGGCGGCGACGGCGACCAGGGCACGTCGATGTGGAGCCACCAGAGCCTGTACAGCGGGAGCTCCGGCACCGAGGAGGCCAGGCGGGAGTTGCCGGAGAAGGGCAACGACAGCGTCGGCAGCAGCGGCGGCGACGACGACGCCGCGGACGACGGCAAGGACAGCGGGAAGGGGGCAGCCTCCGACATGTCGGGCCTGTTCGCCTCCGACTGCGTGCTCTGGGACTTGCCCGACGAGCTCACGAATCACATGGTGTAGCTAAGCTATAAAACGCGGGAAAACTTAGCACGGCGAAGCACGCCATGATCCACATCACTTGCAGTTGCAGGTGGTGAGAATTGGATAGAGCGAAAAATTGCTACCAATAATATTGCTGAAAGAGTGACAGAAAAAGTGAAAAACTATAGTATGTAGCTGGCAAGAAAAATGGGGATTCTTTTTGCTTGGAAAAGAGAAAAAAAAGTGAGATGTGAATAGTTTTACCGAGAAGCA
CDS Sequence
  • >LOC_Os04g39470.1
    ATGGGGCGGGTGCCGTGCTGCGAGAAGGACAACGTGAAGCGCGGGCAGTGGACGCCCGAGGAGGACAACAAGCTGCTCTCCTACATCACCCAGTACGGCACCCGCAACTGGCGCCTCATCCCCAAGAACGCCGGGTTGCAGCGGTGCGGGAAGAGCTGCCGGCTGCGGTGGACCAACTACCTCCGGCCCGACCTCAAGCACGGCGAGTTCACCGACGCCGAGGAGCAGACCATCATCAAGCTCCACTCCGTCGTCGGCAACAGGTGGTCGGTGATCGCGGCGCAGCTTCCGGGGCGGACGGACAACGACGTGAAGAACCACTGGAACACGAAGCTGAAGAAGAAGCTGTCCGGGATGGGCATCGACCCCGTCACGCACAAGTCCTTCTCGCACCTCATGGCCGAGATCGCCACCACGCTGGCGCCGCCGCAGGTGGCGCACCTCGCCGAGGCCGCGCTGGGGTGCTTCAAGGACGAGATGCTCCACCTCCTCACCAAGAAGCGCCCCTCCGACTTCCCCTCGCCCGCCGTGCACGACGGCGCCGGCGCCGGCGCCAGCGCGTCCGCGCTCGCCGCGCCCTGTTTCCCCGCCGCGCCGCCGCACCACCCGCAGGCCGACGACACCATCGAGCGCATCAAGCTCGGCCTGTCCCGCGCCATCATGAGCGATCCCTCCACCGCCTCCGCCGCCGCCGCCGCCGCCGCGCCCTCCGCCCCCGCGGAGGACAAGCCGTGGCCGCCCGGCGACATGTCCGAGGGGCTCGCCGGGATGTACGCCACGTACAACCCGGCGGCGCACGCGCACGCGCAGGCCCAGGCCGAGTTCCGGTACGACGGGGCCTCCGCGGCGCAGGGCTACGTCCTCGGCGGCGACGGCGACCAGGGCACGTCGATGTGGAGCCACCAGAGCCTGTACAGCGGGAGCTCCGGCACCGAGGAGGCCAGGCGGGAGTTGCCGGAGAAGGGCAACGACAGCGTCGGCAGCAGCGGCGGCGACGACGACGCCGCGGACGACGGCAAGGACAGCGGGAAGGGGGCAGCCTCCGACATGTCGGGCCTGTTCGCCTCCGACTGCGTGCTCTGGGACTTGCCCGACGAGCTCACGAATCACATGGTGTAG
Protein Sequence
  • >LOC_Os04g39470.1
    MGRVPCCEKDNVKRGQWTPEEDNKLLSYITQYGTRNWRLIPKNAGLQRCGKSCRLRWTNYLRPDLKHGEFTDAEEQTIIKLHSVVGNRWSVIAAQLPGRTDNDVKNHWNTKLKKKLSGMGIDPVTHKSFSHLMAEIATTLAPPQVAHLAEAALGCFKDEMLHLLTKKRPSDFPSPAVHDGAGAGASASALAAPCFPAAPPHHPQADDTIERIKLGLSRAIMSDPSTASAAAAAAAPSAPAEDKPWPPGDMSEGLAGMYATYNPAAHAHAQAQAEFRYDGASAAQGYVLGGDGDQGTSMWSHQSLYSGSSGTEEARRELPEKGNDSVGSSGGDDDAADDGKDSGKGAASDMSGLFASDCVLWDLPDELTNHMV*