Information report for OsMYB80;BM1
Gene Details
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Functional Descriptions
- OsMYB80 regulates anther development and pollen fertility by targeting multiple biological pathways.
- To understand how OsMYB80 regulates anther development, RNA-seq analysis was conducted to identify genes differentially regulated by OsMYB80 in rice anthers.
- The OsMYB80 mutants exhibited premature tapetum cell death, lack of Ubisch bodies, no exine, and microspore degeneration.
- Here we isolated two allelic male sterile mutants of OsMYB80 and investigated how OsMYB80 regulates male fertility in rice.
- Functional annotation of the 188 genes suggested that OsMYB80 regulates male fertility by directly targeting multiple biological processes.
- Moreover, BM1 affects the expression of several known genes related to tapetum and microspore development.
- Collectively, our results suggest that BM1 is one of key regulators for rice male fertility and may serve as a potential target for rice male-sterile line breeding and hybrid seed production.
- The BM1 mutant exhibited slightly lagging meiosis, aborted transition of the tapetum to a secretory type, premature tapetal degeneration, and abnormal pollen exine formation, leading to ultimately lacks of visible pollens in the mature white anthers.
- Map-based cloning, complementation and targeted mutagenesis using CRISPR/Cas9 technology demonstrated that the mutated LOC_Os04g39470 is the causal gene in BM1.
- Results: In this study, we obtained several lines in which the MYB transcription factor OsMS188 was knocked out through the CRISPR-Cas9 approach.
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice.
- The OsMS188 lines exhibited a male-sterile phenotype with aberrant development and degeneration of tapetal cells, absence of the sexine layer and defective anther cuticles.
- Conclusion: Overall, OsMS188 is a key regulator of tapetum development and pollen wall formation.
Functional Keywords
- pollen , anther , development , cell-death , sterile , fertility , tapetum , microspore , anther-development , seed , map-based-cloning , breeding , tapetal , pollen-exine-formation , transcription-factor , pollen-wall
Literature and News
- MYB80, a regulator of tapetal and pollen development, is functionally conserved in crops . DOI: 10.1007/s11103-011-9855-0 ; PMID: 22086333
- MYB80 homologues in Arabidopsis, cotton and Brassica: regulation and functional conservation in tapetal and pollen development . DOI: 10.1186/s12870-014-0278-3 ; PMID: 25311582
- OsMYB80 Regulates Anther Development and Pollen Fertility by Targeting Multiple Biological Pathways . DOI: 10.1093/pcp/pcaa025 ; PMID: 32142141
- The MYB transcription factor Baymax1 plays a critical . DOI: 10.1007/s00122-020-03706-w ; PMID: 33089345
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice . DOI: 10.1186/s12284-020-00451-y ; PMID: 33409767
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice . DOI: 10.1186/s12284-020-00451-y ; PMID: 33409767
Gene Resources
- UniProt: Q7XQN1
- EMBL: AL606441, AP014960
- AlphaFoldDB: Q7XQN1
- EnsemblPlants: Os04t0470600-01
- Gramene: Os04t0470600-01
- KEGG: osa:9272494
- InterPro: IPR001005, IPR009057, IPR015495
- PANTHER: PTHR47994, PTHR47994:SF2
- SUPFAM: SSF46689
- PROSITE: PS51294
- Gene3D: 1.10.10.60
- OrthoDB: Q7XQN1
- SWISS-MODEL: Q7XQN1
- Conserved Domain Database: cd00167
Sequences
cDNA Sequence
- >LOC_Os04g39470.1
ATTTATAACACGCGGCGCGGCCGTGTGAGCGCCGCACGCACGCAGCAGCAGCGGAATCTCGACGGCGGCGACATCATGGGGCGGGTGCCGTGCTGCGAGAAGGACAACGTGAAGCGCGGGCAGTGGACGCCCGAGGAGGACAACAAGCTGCTCTCCTACATCACCCAGTACGGCACCCGCAACTGGCGCCTCATCCCCAAGAACGCCGGGTTGCAGCGGTGCGGGAAGAGCTGCCGGCTGCGGTGGACCAACTACCTCCGGCCCGACCTCAAGCACGGCGAGTTCACCGACGCCGAGGAGCAGACCATCATCAAGCTCCACTCCGTCGTCGGCAACAGGTGGTCGGTGATCGCGGCGCAGCTTCCGGGGCGGACGGACAACGACGTGAAGAACCACTGGAACACGAAGCTGAAGAAGAAGCTGTCCGGGATGGGCATCGACCCCGTCACGCACAAGTCCTTCTCGCACCTCATGGCCGAGATCGCCACCACGCTGGCGCCGCCGCAGGTGGCGCACCTCGCCGAGGCCGCGCTGGGGTGCTTCAAGGACGAGATGCTCCACCTCCTCACCAAGAAGCGCCCCTCCGACTTCCCCTCGCCCGCCGTGCACGACGGCGCCGGCGCCGGCGCCAGCGCGTCCGCGCTCGCCGCGCCCTGTTTCCCCGCCGCGCCGCCGCACCACCCGCAGGCCGACGACACCATCGAGCGCATCAAGCTCGGCCTGTCCCGCGCCATCATGAGCGATCCCTCCACCGCCTCCGCCGCCGCCGCCGCCGCCGCGCCCTCCGCCCCCGCGGAGGACAAGCCGTGGCCGCCCGGCGACATGTCCGAGGGGCTCGCCGGGATGTACGCCACGTACAACCCGGCGGCGCACGCGCACGCGCAGGCCCAGGCCGAGTTCCGGTACGACGGGGCCTCCGCGGCGCAGGGCTACGTCCTCGGCGGCGACGGCGACCAGGGCACGTCGATGTGGAGCCACCAGAGCCTGTACAGCGGGAGCTCCGGCACCGAGGAGGCCAGGCGGGAGTTGCCGGAGAAGGGCAACGACAGCGTCGGCAGCAGCGGCGGCGACGACGACGCCGCGGACGACGGCAAGGACAGCGGGAAGGGGGCAGCCTCCGACATGTCGGGCCTGTTCGCCTCCGACTGCGTGCTCTGGGACTTGCCCGACGAGCTCACGAATCACATGGTGTAGCTAAGCTATAAAACGCGGGAAAACTTAGCACGGCGAAGCACGCCATGATCCACATCACTTGCAGTTGCAGGTGGTGAGAATTGGATAGAGCGAAAAATTGCTACCAATAATATTGCTGAAAGAGTGACAGAAAAAGTGAAAAACTATAGTATGTAGCTGGCAAGAAAAATGGGGATTCTTTTTGCTTGGAAAAGAGAAAAAAAAGTGAGATGTGAATAGTTTTACCGAGAAGCA
CDS Sequence
- >LOC_Os04g39470.1
ATGGGGCGGGTGCCGTGCTGCGAGAAGGACAACGTGAAGCGCGGGCAGTGGACGCCCGAGGAGGACAACAAGCTGCTCTCCTACATCACCCAGTACGGCACCCGCAACTGGCGCCTCATCCCCAAGAACGCCGGGTTGCAGCGGTGCGGGAAGAGCTGCCGGCTGCGGTGGACCAACTACCTCCGGCCCGACCTCAAGCACGGCGAGTTCACCGACGCCGAGGAGCAGACCATCATCAAGCTCCACTCCGTCGTCGGCAACAGGTGGTCGGTGATCGCGGCGCAGCTTCCGGGGCGGACGGACAACGACGTGAAGAACCACTGGAACACGAAGCTGAAGAAGAAGCTGTCCGGGATGGGCATCGACCCCGTCACGCACAAGTCCTTCTCGCACCTCATGGCCGAGATCGCCACCACGCTGGCGCCGCCGCAGGTGGCGCACCTCGCCGAGGCCGCGCTGGGGTGCTTCAAGGACGAGATGCTCCACCTCCTCACCAAGAAGCGCCCCTCCGACTTCCCCTCGCCCGCCGTGCACGACGGCGCCGGCGCCGGCGCCAGCGCGTCCGCGCTCGCCGCGCCCTGTTTCCCCGCCGCGCCGCCGCACCACCCGCAGGCCGACGACACCATCGAGCGCATCAAGCTCGGCCTGTCCCGCGCCATCATGAGCGATCCCTCCACCGCCTCCGCCGCCGCCGCCGCCGCCGCGCCCTCCGCCCCCGCGGAGGACAAGCCGTGGCCGCCCGGCGACATGTCCGAGGGGCTCGCCGGGATGTACGCCACGTACAACCCGGCGGCGCACGCGCACGCGCAGGCCCAGGCCGAGTTCCGGTACGACGGGGCCTCCGCGGCGCAGGGCTACGTCCTCGGCGGCGACGGCGACCAGGGCACGTCGATGTGGAGCCACCAGAGCCTGTACAGCGGGAGCTCCGGCACCGAGGAGGCCAGGCGGGAGTTGCCGGAGAAGGGCAACGACAGCGTCGGCAGCAGCGGCGGCGACGACGACGCCGCGGACGACGGCAAGGACAGCGGGAAGGGGGCAGCCTCCGACATGTCGGGCCTGTTCGCCTCCGACTGCGTGCTCTGGGACTTGCCCGACGAGCTCACGAATCACATGGTGTAG
Protein Sequence
- >LOC_Os04g39470.1
MGRVPCCEKDNVKRGQWTPEEDNKLLSYITQYGTRNWRLIPKNAGLQRCGKSCRLRWTNYLRPDLKHGEFTDAEEQTIIKLHSVVGNRWSVIAAQLPGRTDNDVKNHWNTKLKKKLSGMGIDPVTHKSFSHLMAEIATTLAPPQVAHLAEAALGCFKDEMLHLLTKKRPSDFPSPAVHDGAGAGASASALAAPCFPAAPPHHPQADDTIERIKLGLSRAIMSDPSTASAAAAAAAPSAPAEDKPWPPGDMSEGLAGMYATYNPAAHAHAQAQAEFRYDGASAAQGYVLGGDGDQGTSMWSHQSLYSGSSGTEEARRELPEKGNDSVGSSGGDDDAADDGKDSGKGAASDMSGLFASDCVLWDLPDELTNHMV*