Information report for BAD1;OsBADH1
Gene Details
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Functional Descriptions
- Northern blot analysis revealed that salt-tolerant in each rice cultivar is correlated to the expression level of OsBADH1 mRNA.
- Moreover, these results suggest that the expression of OsBADH1 gene in response to salt stress could be magnified under high light conditions.
- Interestingly, the effect of salt stress on the expression of OsBADH1 gene was alleviated by CO2 enrichment.
- Expression of OsBADH1 gene in Indica rice (Oryza sativaL.) in correlation with salt, plasmolysis, temperature and light stresses.
- We previously found that the expression of betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance of rice.
- In this study, the expression of the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach.
- The expression studies showed that OsBADH1 can be induced by a variety of environmental factors such as salinity, drought, cold, heat, light intensity and CO2 concentration.
- The results demonstrated that the OsBADH1 mRNA expression was up-regulated by salinity, drought, cold and high light intensity but down-regulated by CO2 enrichment and heat stress.
- The primary response of OsBADH1 gene expression was induced within 24 h after salinity, cold or drought stress treatment.
- Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco.
- Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator.
- Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance.
- The accumulation of OsBADH1 mRNA decreases following submergence treatment, but quickly recovers after re-aeration.
- Transgenic rice lines downregulating OsBADH1 exhibited remarkably reduced tolerance to NaCl, drought and cold stresses.
- In this study, we demonstrated a pivotal role of OsBADH1 in stress tolerance without altering GB biosynthesis capacity, using the RNA interference (RNAi) technique.
Functional Keywords
- salt , drought , salinity , temperature , salt-stress , salt-tolerance , salinity-stress , submergence , tolerance , stress
Literature and News
- OsBADH1 is possibly involved in acetaldehyde oxidation in rice plant peroxisomes . DOI: 10.1016/j.febslet.2009.10.039 ; PMID: 19850038
- Dissecting substrate specificity of two rice BADH isoforms: Enzyme kinetics, docking and molecular dynamics simulation studies . DOI: 10.1016/j.biochi.2012.04.009 ; PMID: 22534193
- Genomic insight into genetic changes and shaping of major inbred rice cultivars in China . DOI: 10.1111/nph.18500 ; PMID: 36114658
- Expression of OsBADH1 gene in Indica rice (Oryza sativa L.) in correlation with salt, plasmolysis, temperature and light stresses . DOI: 10.1007/s11816-009-0123-6
- Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice . DOI: 10.1007/s11103-008-9381-x ; PMID: 18704694
- Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco . DOI: 10.1007/s11816-009-0123-6
- RNAi-directed downregulation of betaine aldehyde dehydrogenase 1 (OsBADH1) results in decreased stress tolerance and increased oxidative markers without affecting glycine betaine biosynthesis in rice (Oryza sativa) . DOI: 10.1007/s11103-014-0239-0 ; PMID: 25150410
- Resequencing Reveals Different Domestication Rate for BADH1 and BADH2 in Rice (Oryza sativa) . DOI: 10.1371/journal.pone.0134801 ; PMID: 26258482
Gene Resources
- UniProt: O24174
- EMBL: AB001348, AK103582, AL663017
- AlphaFoldDB: O24174
- EnsemblPlants: Os04t0464200-01
- Gramene: Os04t0464200-01
- KEGG: dosa:Os04g0464200
- Orthologous matrix: KHVMFDN
- InterPro: IPR015590, IPR016160, IPR016161
- PANTHER: PTHR43860, PTHR43860:SF7
- SUPFAM: SSF53720
- PROSITE: PS00070, PS00687
- Gene3D: 3.40.309.10, 3.40.605.10
- OrthoDB: O24174
- SWISS-MODEL: O24174
- Conserved Domain Database: cd07110
- eggNOG: KOG2450
Sequences
cDNA Sequence
- >LOC_Os04g39020.1
AGTGGCGTTTTTCCTTTTTTTTTTGCTCTCATCAATCGGTGAGAGAGAGTGCTCGTCGTCGATCGCCCAAGGTCCGGGACAACCCAAAAGGCCGCGGCGACAGCGGAGCAGCAGCAGCAGCAGCTCGCGCCGCCCCCCAACCGGAAGCCATGGCCGCGCCGTCGGCGATCCCCCGCCGCGGCCTGTTCATCGGCGGCGGGTGGCGGGAGCCGTCCCTCGGCCGCCGCCTCCCCGTCGTCAACCCGGCCACGGAGGCAACCATCGGTGACATCCCGGCGGCCACGGCGGAGGACGTCGAGCTCGCGGTGTCGGCGGCGAGGGATGCGTTCGGCCGCGACGGTGGGAGACACTGGTCGCGCGCGCCTGGGGCCGTGCGGGCCAAGTACCTCAAGGCGATCGCCGCTAAGATTAAAGATAAGAAATCTTATCTAGCTTTGTTGGAGACTCTTGATTCTGGGAAGCCTCTGGATGAAGCAGCTGGGGACATGGAGGATGTCGCTGCATGCTTTGAGTATTATGCTGATCTGGCAGAAGCTTTAGATGGGAAACAACGGGCACCAATCTCTCTACCCATGGAAAATTTTGAGTCCTATGTACTCAAAGAACCCATTGGGGTTGTTGGACTTATCACTCCCTGGAATTATCCTCTGCTGATGGCTACTTGGAAGGTTGCACCTGCCCTGGCTGCTGGGTGTACAGCTGTATTAAAGCCATCTGAGCTTGCTTCCCTGACATGTTTAGAGCTTGGTGGAATATGTGCAGAAATTGGATTACCTCCAGGAGTCTTGAACATAATTACTGGTCTGGGCACTGAAGCTGGTGCTCCATTAGCTTCACATCCCCATGTGGATAAGATTGCTTTTACTGGAAGCACAGAAACTGGTAAGAGGATAATGATTACTGCTTCCCAAATGGTCAAGCCTGTTTCGTTAGAGCTTGGTGGCAAAAGTCCTCTTATTGTCTTTGATGATGTTGATATTGATAAAGCTGTTGAGTGGGCCATGTTTGGGTGTTTTGCGAACGCTGGTCAAGTCTGCAGTGCTACTTCTCGTCTACTTTTGCATGAGAAAATTGCAAAGCGATTCTTGGATAGGTTGGTTGCATGGGCAAAGAGTATCAAAATCTCAGATCCACTAGAAGAAGGTTGCAGGCTGGGGTCAGTCGTTAGTGAAGGGCAGTATCAAAAAATAATGAAGTTCATCTCAACAGCAAGATGTGAAGGTGCCACAATTCTATATGGGGGTGCCCGACCACAACACCTCAAAAGGGGGTTCTTTATTGAGCCTACTATTATAACAAATGTTAGCACATCAATGCAAATTTGGCGAGAGGAAGTCTTTGGACCGGTCATCTGCGTTAAAGAATTTAGGACAGAGCGTGAAGCAGTAGAACTTGCAAATGATACTCACTATGGTCTAGCTGGCGCTGTGATTTCCAATGATCTAGAGAGGTGCGAGCGCATTTCAAAGGCTATCCAGTCAGGTATCGTTTGGATAAATTGCTCGCAACCATGCTTTGTTCAAGCTCCATGGGGAGGGAACAAGCGGAGTGGTTTTGGCCGGGAGCTAGGACAGTGGGGCCTCGATAACTACTTGAGCGTGAAGCAAGTCACCAAGTACTGCTCAGATGAACCATACGGATGGTACCGGCCTCCATCCAAGCTGTAGATTTGTCATTCGTGTCAAGAGCGACGGAGCACGTCATTACCTGAAAGCAAGATCAACCCTGTACATTCAGAACGGACGGACAGAATCTTCTTTACAGTTGCTCTTACGGTCTTACAATAAGCCTTGCATGGCAATAGCAACTTATGAATTACAGTATATGATACTGCGTCATCAAGCAAGCCATGTGTGTGCTGTATCAGACTATCATTCGCTTTGTCGAGCACCTGCATTTGTCACCACTCACCACTGTTAAAGCAAGAATGCAAAATGCAAAATGAAAAAGAAAACTGCAGTATGCTGTGTTTATGTGGTTACCTGCTAGGCTGCTACTCTGTCTATCTGCATTTTTTTGAACCATCATGAATTG
CDS Sequence
- >LOC_Os04g39020.1
ATGGCCGCGCCGTCGGCGATCCCCCGCCGCGGCCTGTTCATCGGCGGCGGGTGGCGGGAGCCGTCCCTCGGCCGCCGCCTCCCCGTCGTCAACCCGGCCACGGAGGCAACCATCGGTGACATCCCGGCGGCCACGGCGGAGGACGTCGAGCTCGCGGTGTCGGCGGCGAGGGATGCGTTCGGCCGCGACGGTGGGAGACACTGGTCGCGCGCGCCTGGGGCCGTGCGGGCCAAGTACCTCAAGGCGATCGCCGCTAAGATTAAAGATAAGAAATCTTATCTAGCTTTGTTGGAGACTCTTGATTCTGGGAAGCCTCTGGATGAAGCAGCTGGGGACATGGAGGATGTCGCTGCATGCTTTGAGTATTATGCTGATCTGGCAGAAGCTTTAGATGGGAAACAACGGGCACCAATCTCTCTACCCATGGAAAATTTTGAGTCCTATGTACTCAAAGAACCCATTGGGGTTGTTGGACTTATCACTCCCTGGAATTATCCTCTGCTGATGGCTACTTGGAAGGTTGCACCTGCCCTGGCTGCTGGGTGTACAGCTGTATTAAAGCCATCTGAGCTTGCTTCCCTGACATGTTTAGAGCTTGGTGGAATATGTGCAGAAATTGGATTACCTCCAGGAGTCTTGAACATAATTACTGGTCTGGGCACTGAAGCTGGTGCTCCATTAGCTTCACATCCCCATGTGGATAAGATTGCTTTTACTGGAAGCACAGAAACTGGTAAGAGGATAATGATTACTGCTTCCCAAATGGTCAAGCCTGTTTCGTTAGAGCTTGGTGGCAAAAGTCCTCTTATTGTCTTTGATGATGTTGATATTGATAAAGCTGTTGAGTGGGCCATGTTTGGGTGTTTTGCGAACGCTGGTCAAGTCTGCAGTGCTACTTCTCGTCTACTTTTGCATGAGAAAATTGCAAAGCGATTCTTGGATAGGTTGGTTGCATGGGCAAAGAGTATCAAAATCTCAGATCCACTAGAAGAAGGTTGCAGGCTGGGGTCAGTCGTTAGTGAAGGGCAGTATCAAAAAATAATGAAGTTCATCTCAACAGCAAGATGTGAAGGTGCCACAATTCTATATGGGGGTGCCCGACCACAACACCTCAAAAGGGGGTTCTTTATTGAGCCTACTATTATAACAAATGTTAGCACATCAATGCAAATTTGGCGAGAGGAAGTCTTTGGACCGGTCATCTGCGTTAAAGAATTTAGGACAGAGCGTGAAGCAGTAGAACTTGCAAATGATACTCACTATGGTCTAGCTGGCGCTGTGATTTCCAATGATCTAGAGAGGTGCGAGCGCATTTCAAAGGCTATCCAGTCAGGTATCGTTTGGATAAATTGCTCGCAACCATGCTTTGTTCAAGCTCCATGGGGAGGGAACAAGCGGAGTGGTTTTGGCCGGGAGCTAGGACAGTGGGGCCTCGATAACTACTTGAGCGTGAAGCAAGTCACCAAGTACTGCTCAGATGAACCATACGGATGGTACCGGCCTCCATCCAAGCTGTAG
Protein Sequence
- >LOC_Os04g39020.1
MAAPSAIPRRGLFIGGGWREPSLGRRLPVVNPATEATIGDIPAATAEDVELAVSAARDAFGRDGGRHWSRAPGAVRAKYLKAIAAKIKDKKSYLALLETLDSGKPLDEAAGDMEDVAACFEYYADLAEALDGKQRAPISLPMENFESYVLKEPIGVVGLITPWNYPLLMATWKVAPALAAGCTAVLKPSELASLTCLELGGICAEIGLPPGVLNIITGLGTEAGAPLASHPHVDKIAFTGSTETGKRIMITASQMVKPVSLELGGKSPLIVFDDVDIDKAVEWAMFGCFANAGQVCSATSRLLLHEKIAKRFLDRLVAWAKSIKISDPLEEGCRLGSVVSEGQYQKIMKFISTARCEGATILYGGARPQHLKRGFFIEPTIITNVSTSMQIWREEVFGPVICVKEFRTEREAVELANDTHYGLAGAVISNDLERCERISKAIQSGIVWINCSQPCFVQAPWGGNKRSGFGRELGQWGLDNYLSVKQVTKYCSDEPYGWYRPPSKL*