Gene Details:

Functional Descriptions:

  • Regenerated TDD1 plants showed pleiotropic phenotypes: dwarfing, narrow leaves, short roots and abnormal flowers.
  • Trp feeding completely rescued the mutant phenotypes and moderate expression of OsYUCCA1, which encodes a key enzyme in Trp-dependent IAA biosynthesis, also rescued plant height and root length, indicating that the abnormal phenotypes of TDD1 are caused predominantly by Trp and IAA deficiency.
  • TDD1-uidA and DR5-uidA expression overlapped at many sites in WT plants but was lacking in TDD1, indicating that TDD1 is involved in auxin biosynthesis.
  • Both Trp and IAA levels in flowers and embryos were much lower in TDD1 than in wild type (WT).
  • A rice tryptophan deficient dwarf mutant, TDD1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos.
  • Here, we report the analysis of a rice tryptophan- (Trp-) and IAA-deficient mutant, tryptophan deficient dwarf1 (TDD1), which is embryonic lethal because of a failure to develop most organs during embryogenesis.
  • TDD1 encodes a protein homologous to anthranilate synthase beta-subunit, which catalyses the first step of the Trp biosynthesis pathway and functions upstream of Trp-dependent IAA biosynthesis.
  • In TDD1 embryos, the expression patterns of OSH1 and OsSCR, which mark the presumptive apical region and the L2 layer, respectively, are identical to those in WT, suggesting a possibility either that different IAA levels are required for basic pattern formation than for organ formation or that an orthologous gene compensates for TDD1 deficiency during pattern formation.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os04g38950.1
    CCTCGCACATCCACTCCTCCCCCCCAAAACCACACAAACACCCGCTGCGCCGCTCGCTTCCTCCTCCCCCTCCTCGTGCTCGACATGGCGTGCTCCCACCTGGCCGCCGCCGCCGCCGCGGCCTCCCCGGCGGCCGCGCGTTCACCGGCGGCCTCCAGCGCCGCAACCGCGAGCGCCTTCGCGCGCCTCTCGGCGACGCCCCGGGTCGCGAGCGGCGGGTTGGCCGTTAGGGGCCAGAGGGGTGTAGCCGCTGTTGTCGCCGCCGCCGCCGGGGCCGCCGCGGCGACGCCCGTGGCCGACATCGAGGAACGCCGGGCCACCGAGAAGCAGCCCATCATTGTCATCGATAACTACGACAGCTTTACCTACAACCTCTGCCAGTATATGGGGGAGCTTGGATTGAACTTTGAGGTATATCGCAATGATGAACTTACCATAGAGGATGTAAAGAGGAAGAACCCAAGAGGAATACTTATTTCTCCAGGGCCTGGTGAGCCACAAGATTCAGGTATATCATTGCAGACTGTTCTGGAACTTGGACCTACCATCCCAATTTTTGGTGTCTGCATGGGTCTGCAGTGTATCGGGGAAGCTTTTGGAGGAAAGATTATCCGTGCTCCTTCTGGTGTCATGCATGGAAAAAGCTCTCCAGTTCGCTACGATGAGGAGCTAGGGAAGGCCTTGTTCAATGGCTTGCCAAACCCATTTACCGCTGCAAGATACCATAGCTTGGTGATCGAGCAGGAGACCTTCCCCCATGACGCTCTGGAAGCCACCGCGTGGACTGAAGATGGCCTTATCATGGCTGCTCGCCACAAGAAGTACAGGCACATCCAGGGAGTCCAATTCCACCCAGAGAGCATCATCACCCCAGAAGGCAAGAGGATCATTCTCAACTTCGTGAGGTTCATCGAGGAGCTGGAGAAGCAGCGTGCCGGAGAGAAGAACTAGTAGACGCTAGCTGTTCAGGCAAAAGTAGAAAGGGGCTTAAGCCGGGTAGATTCAGGGCAGAAAGTGAAAAAGGGTAGGAGTAATAAGGGAAAAAGAGCTCTCCTTTTGTCACATTCGTTTGGTTCGTTGTAATATATATCCTTTGTGGATCTTGAATAAGCTCTATCCTGTCCTTAAATTCGTGGTGCTA
  • >LOC_Os04g38950.2
    CCTCGCACATCCACTCCTCCCCCCCAAAACCACACAAACACCCGCTGCGCCGCTCGCTTCCTCCTCCCCCTCCTCGTGCTCGACATGGCGTGCTCCCACCTGGCCGCCGCCGCCGCCGCGGCCTCCCCGGCGGCCGCGCGTTCACCGGCGGCCTCCAGCGCCGCAACCGCGAGCGCCTTCGCGCGCCTCTCGGCGACGCCCCGGGTCGCGAGCGGCGGGTTGGCCGTTAGGGGCCAGAGGGGTGTAGCCGCTGTTGTCGCCGCCGCCGCCGGGGCCGCCGCGGCGACGCCCGTGGCCGACATCGAGGAACGCCGGGCCACCGAGAAGCAGCCCATCATTGTCATCGATAACTACGACAGCTTTACCTACAACCTCTGCCAGTATATGGGGGAGCTTGGATTGAACTTTGAGGTATATCGCAATGATGAACTTACCATAGAGGATGTAAAGAGGAAGAACCCAAGAGGAATACTTATTTCTCCAGGGCCTGGTGAGCCACAAGATTCAGGTATATCATTGCAGACTGTTCTGGAACTTGGACCTACCATCCCAATTTTTGGTGTCTGCATGGGTCTGCAGTGTATCGGGGAAGCTTTTGGAGGAAAGATTATCCGTGCTCCTTCTGGTGTCATGCATGGAAAAAGCTCTCCAGTTCGCTACGATGAGGAGCTAGGGAAGGCCTTGTTCAATGGCTTGCCAAACCCATTTACCGCTGCAAGATACCATAGCTTGGTGATCGAGCAGGAGACCTTCCCCCATGACGCTCTGGAAGCCACCGCGTGGACTGAAGATGGCCTTATCATGGCTGCTCGCCACAAGAAGTACAGGCACATCCAGGGAGTCCAATTCCACCCAGAGAGCATCATCACCCCAGAAGGCAAGAGGATCATTCTCAACTTCGTGAGGTTCATCGAGGAGCTGGAGAAGCAGCGTGCCGGAGAGAAGAACTAGTAGACGCTAGCTGTTCAGGCAAAAGTAGAAAGGGGCTTAAGCCGGGTAGATTCAGGGCAGAAAGTGAAAAAGGGTAGGAGTAATAAGGGAAAAAGAGCTCTCCTTTTGTCACATTCGTTTGGTTCGTTGTAATATATATCCTTTGTGGATCTTGAATAAGCTCTATCCTGTCCTTAAATTCGTGGTGCTA
  • >LOC_Os04g38950.3
    CCTCGCACATCCACTCCTCCCCCCCAAAACCACACAAACACCCGCTGCGCCGCTCGCTTCCTCCTCCCCCTCCTCGTGCTCGACATGGCGTGCTCCCACCTGGCCGCCGCCGCCGCCGCGGCCTCCCCGGCGGCCGCGCGTTCACCGGCGGCCTCCAGCGCCGCAACCGCGAGCGCCTTCGCGCGCCTCTCGGCGACGCCCCGGGTCGCGAGCGGCGGGTTGGCCGTTAGGGGCCAGAGGGGTGTAGCCGCTGTTGTCGCCGCCGCCGCCGGGGCCGCCGCGGCGACGCCCGTGGCCGACATCGAGGAACGCCGGGCCACCGAGAAGCAGCCCATCATTGTCATCGATAACTACGACAGCTTTACCTACAACCTCTGCCAGTATATGGGGGAGCTTGGATTGAACTTTGAGGTATATCGCAATGATGAACTTACCATAGAGGATGTAAAGAGGAAGAACCCAAGAGGAATACTTATTTCTCCAGGGCCTGGTGAGCCACAAGATTCAGGTATATCATTGCAGACTGTTCTGGAACTTGGACCTACCATCCCAATTTTTGGTGTCTGCATGGGTCTGCAGTGTATCGGGGAAGCTTTTGGAGGAAAGATTATCCGTGCTCCTTCTGGTGTCATGCATGGAAAAAGCTCTCCAGTTCGCTACGATGAGGAGCTAGGGAAGGCCTTGTTCAATGGCTTGCCAAACCCATTTACCGCTGCAAGATACCATAGCTTGGTGATCGAGCAGGAGACCTTCCCCCATGACGCTCTGGAAGCCACCGCGTGGACTGAAGATGGCCTTATCATGGCTGCTCGCCACAAGAAGTACAGGCACATCCAGGGAGTCCAATTCCACCCAGAGAGCATCATCACCCCAGAAGGCAAGAGGATCATTCTCAACTTCGTGAGGTTCATCGAGGAGCTGGAGAAGCAGCGTGCCGGAGAGAAGAACTAGTAGACGCTAGCTGTTCAGGCAAAAGTAGAAAGGGGCTTAAGCCGGGTAGATTCAGGGCAGAAAGTGAAAAAGGGTAGGAGTAATAAGGGAAAAAGAGCTCTCCTTTTGTCACATTCGTTTGGTTCGTTGTAATATATATCCTTTGTGGATCTTGAATAAGCTCTATCCTGTCCTTAAATTCGTGGTGCTA
CDS Sequence
  • >LOC_Os04g38950.1
    ATGGCGTGCTCCCACCTGGCCGCCGCCGCCGCCGCGGCCTCCCCGGCGGCCGCGCGTTCACCGGCGGCCTCCAGCGCCGCAACCGCGAGCGCCTTCGCGCGCCTCTCGGCGACGCCCCGGGTCGCGAGCGGCGGGTTGGCCGTTAGGGGCCAGAGGGGTGTAGCCGCTGTTGTCGCCGCCGCCGCCGGGGCCGCCGCGGCGACGCCCGTGGCCGACATCGAGGAACGCCGGGCCACCGAGAAGCAGCCCATCATTGTCATCGATAACTACGACAGCTTTACCTACAACCTCTGCCAGTATATGGGGGAGCTTGGATTGAACTTTGAGGTATATCGCAATGATGAACTTACCATAGAGGATGTAAAGAGGAAGAACCCAAGAGGAATACTTATTTCTCCAGGGCCTGGTGAGCCACAAGATTCAGGTATATCATTGCAGACTGTTCTGGAACTTGGACCTACCATCCCAATTTTTGGTGTCTGCATGGGTCTGCAGTGTATCGGGGAAGCTTTTGGAGGAAAGATTATCCGTGCTCCTTCTGGTGTCATGCATGGAAAAAGCTCTCCAGTTCGCTACGATGAGGAGCTAGGGAAGGCCTTGTTCAATGGCTTGCCAAACCCATTTACCGCTGCAAGATACCATAGCTTGGTGATCGAGCAGGAGACCTTCCCCCATGACGCTCTGGAAGCCACCGCGTGGACTGAAGATGGCCTTATCATGGCTGCTCGCCACAAGAAGTACAGGCACATCCAGGGAGTCCAATTCCACCCAGAGAGCATCATCACCCCAGAAGGCAAGAGGATCATTCTCAACTTCGTGAGGTTCATCGAGGAGCTGGAGAAGCAGCGTGCCGGAGAGAAGAACTAG
  • >LOC_Os04g38950.2
    ATGGCGTGCTCCCACCTGGCCGCCGCCGCCGCCGCGGCCTCCCCGGCGGCCGCGCGTTCACCGGCGGCCTCCAGCGCCGCAACCGCGAGCGCCTTCGCGCGCCTCTCGGCGACGCCCCGGGTCGCGAGCGGCGGGTTGGCCGTTAGGGGCCAGAGGGGTGTAGCCGCTGTTGTCGCCGCCGCCGCCGGGGCCGCCGCGGCGACGCCCGTGGCCGACATCGAGGAACGCCGGGCCACCGAGAAGCAGCCCATCATTGTCATCGATAACTACGACAGCTTTACCTACAACCTCTGCCAGTATATGGGGGAGCTTGGATTGAACTTTGAGGTATATCGCAATGATGAACTTACCATAGAGGATGTAAAGAGGAAGAACCCAAGAGGAATACTTATTTCTCCAGGGCCTGGTGAGCCACAAGATTCAGGTATATCATTGCAGACTGTTCTGGAACTTGGACCTACCATCCCAATTTTTGGTGTCTGCATGGGTCTGCAGTGTATCGGGGAAGCTTTTGGAGGAAAGATTATCCGTGCTCCTTCTGGTGTCATGCATGGAAAAAGCTCTCCAGTTCGCTACGATGAGGAGCTAGGGAAGGCCTTGTTCAATGGCTTGCCAAACCCATTTACCGCTGCAAGATACCATAGCTTGGTGATCGAGCAGGAGACCTTCCCCCATGACGCTCTGGAAGCCACCGCGTGGACTGAAGATGGCCTTATCATGGCTGCTCGCCACAAGAAGTACAGGCACATCCAGGGAGTCCAATTCCACCCAGAGAGCATCATCACCCCAGAAGGCAAGAGGATCATTCTCAACTTCGTGAGGTTCATCGAGGAGCTGGAGAAGCAGCGTGCCGGAGAGAAGAACTAG
  • >LOC_Os04g38950.3
    ATGGCGTGCTCCCACCTGGCCGCCGCCGCCGCCGCGGCCTCCCCGGCGGCCGCGCGTTCACCGGCGGCCTCCAGCGCCGCAACCGCGAGCGCCTTCGCGCGCCTCTCGGCGACGCCCCGGGTCGCGAGCGGCGGGTTGGCCGTTAGGGGCCAGAGGGGTGTAGCCGCTGTTGTCGCCGCCGCCGCCGGGGCCGCCGCGGCGACGCCCGTGGCCGACATCGAGGAACGCCGGGCCACCGAGAAGCAGCCCATCATTGTCATCGATAACTACGACAGCTTTACCTACAACCTCTGCCAGTATATGGGGGAGCTTGGATTGAACTTTGAGGTATATCGCAATGATGAACTTACCATAGAGGATGTAAAGAGGAAGAACCCAAGAGGAATACTTATTTCTCCAGGGCCTGGTGAGCCACAAGATTCAGGTATATCATTGCAGACTGTTCTGGAACTTGGACCTACCATCCCAATTTTTGGTGTCTGCATGGGTCTGCAGTGTATCGGGGAAGCTTTTGGAGGAAAGATTATCCGTGCTCCTTCTGGTGTCATGCATGGAAAAAGCTCTCCAGTTCGCTACGATGAGGAGCTAGGGAAGGCCTTGTTCAATGGCTTGCCAAACCCATTTACCGCTGCAAGATACCATAGCTTGGTGATCGAGCAGGAGACCTTCCCCCATGACGCTCTGGAAGCCACCGCGTGGACTGAAGATGGCCTTATCATGGCTGCTCGCCACAAGAAGTACAGGCACATCCAGGGAGTCCAATTCCACCCAGAGAGCATCATCACCCCAGAAGGCAAGAGGATCATTCTCAACTTCGTGAGGTTCATCGAGGAGCTGGAGAAGCAGCGTGCCGGAGAGAAGAACTAG
Protein Sequence
  • >LOC_Os04g38950.1
    MACSHLAAAAAAASPAAARSPAASSAATASAFARLSATPRVASGGLAVRGQRGVAAVVAAAAGAAAATPVADIEERRATEKQPIIVIDNYDSFTYNLCQYMGELGLNFEVYRNDELTIEDVKRKNPRGILISPGPGEPQDSGISLQTVLELGPTIPIFGVCMGLQCIGEAFGGKIIRAPSGVMHGKSSPVRYDEELGKALFNGLPNPFTAARYHSLVIEQETFPHDALEATAWTEDGLIMAARHKKYRHIQGVQFHPESIITPEGKRIILNFVRFIEELEKQRAGEKN*
  • >LOC_Os04g38950.2
    MACSHLAAAAAAASPAAARSPAASSAATASAFARLSATPRVASGGLAVRGQRGVAAVVAAAAGAAAATPVADIEERRATEKQPIIVIDNYDSFTYNLCQYMGELGLNFEVYRNDELTIEDVKRKNPRGILISPGPGEPQDSGISLQTVLELGPTIPIFGVCMGLQCIGEAFGGKIIRAPSGVMHGKSSPVRYDEELGKALFNGLPNPFTAARYHSLVIEQETFPHDALEATAWTEDGLIMAARHKKYRHIQGVQFHPESIITPEGKRIILNFVRFIEELEKQRAGEKN*
  • >LOC_Os04g38950.3
    MACSHLAAAAAAASPAAARSPAASSAATASAFARLSATPRVASGGLAVRGQRGVAAVVAAAAGAAAATPVADIEERRATEKQPIIVIDNYDSFTYNLCQYMGELGLNFEVYRNDELTIEDVKRKNPRGILISPGPGEPQDSGISLQTVLELGPTIPIFGVCMGLQCIGEAFGGKIIRAPSGVMHGKSSPVRYDEELGKALFNGLPNPFTAARYHSLVIEQETFPHDALEATAWTEDGLIMAARHKKYRHIQGVQFHPESIITPEGKRIILNFVRFIEELEKQRAGEKN*