Information report for OsRap2.6;OsERF101
Gene Details
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Functional Descriptions
- OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1).
- An ERF transcription factor OsERF101 is predominantly expressed in rice reproductive tissues and plays an important role in improving rice seed setting rate under drought stress.
- OsERF101 was predominantly expressed in flowers, particularly in the tapetum and microspores under normal growth conditions.
- In this study, we identified a gene named OsERF101 as an important positive regulator in the adaptive responses to dehydration stress during the reproductive and vegetative stages.
- Taken together, our results indicate that OsERF101 is a gene that regulates dehydration responses during the vegetative and reproductive stages.
- Compared with wild type, the OsERF101 T-DNA knockout mutant showed delayed leaf yellowing and higher chlorophyll contents during DIS and natural senescence.
- After methyl jasmonate treatment to induce rapid leaf de-greening, the OsERF101 leaves retained more chlorophyll compared with wild type, indicating that OsERF101 is involved in promoting jasmonic acid (JA)-induced leaf senescence.
- Transient transactivation and chromatin immunoprecipitation assays revealed that OsERF101 directly binds to the promoter regions of OsNAP and OsMYC2, which activate genes involved in chlorophyll degradation and JA signaling-mediated leaf senescence.
- These results demonstrate that OsERF101 promotes the onset and progression of leaf senescence through a JA-mediated signaling pathway.
- OsERF101 encodes a predicted transcription factor and OsERF101 transcript levels rapidly increased in rice leaves during dark-induced senescence (DIS), indicating that OsERF101 is a senescence-associated transcription factor.
- Consistent with the involvement of JA, the expression of the JA signaling genes OsMYC2/JA INSENSITIVE 1 (OsJAI1) and CORONATINE INSENSITIVE 1a (OsCOI1a), was downregulated in the OsERF101 leaves during DIS.
- The rice OsERF101 transcription factor regulates the NLR Xa1-mediated immunity induced by perception of TAL effectors.
- Unexpectedly, both the overexpression and the knockout lines of OsERF101 displayed Xa1-dependent, enhanced resistance to an incompatible Xoo strain.
- Xa1 interacts in the nucleus with the TAL effectors and OsERF101 via the BED domain.
- Our results indicate that OsERF101 regulates the recognition of TAL effectors by Xa1, and functions as a positive regulator of Xa1-mediated immunity.
Functional Keywords
- transcription-factor , growth , seed , drought , vegetative , stress , reproductive , tapetum , drought-stress , leaf , leaf-senescence , senescence , jasmonate , ja , JA , jasmonic , jasmonic-acid , chlorophyll-content , methyl-jasmonate , JA-signaling , resistance , nucleus , immunity , xoo , Xoo
Literature and News
- OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1) . DOI: 10.1186/1939-8433-5-35 ; PMID: 24280008
- OsERF101, an ERF family transcription factor, regulates drought stress response in reproductive tissues . DOI: 10.1007/s11103-018-0762-5 ; PMID: 30143992
- Rice ETHYLENE RESPONSE FACTOR 101 Promotes Leaf Senescence Through Jasmonic Acid-Mediated Regulation of OsNAP and OsMYC2 . DOI: 10.3389/fpls.2020.01096 ; PMID: 32765572
- A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1 . DOI: 10.3389/fpls.2021.802758 ; PMID: 34956298
- The rice OsERF101 transcription factor regulates the NLR Xa1-mediated immunity induced by perception of TAL effectors . DOI: 10.1111/nph.18439 ; PMID: 36050871
Gene Resources
- UniProt: Q7XLG6
- EMBL: AK101501, AP014960
- AlphaFoldDB: Q7XLG6
- EnsemblPlants: Os04t0398000-01, Os04t0398000-02
- Gramene: Os04t0398000-01, Os04t0398000-02
- KEGG: osa:4335707
- Orthologous matrix: EIEEHRY
- InterPro: IPR001471, IPR016177, IPR036955
- PANTHER: PTHR31190, PTHR31190:SF489
- SUPFAM: SSF54171
- PROSITE: PS51032
- Gene3D: 3.30.730.10
- OrthoDB: Q7XLG6
- SWISS-MODEL: Q7XLG6
- Conserved Domain Database: cd00018
- eggNOG: ENOG502RZR5
Sequences
cDNA Sequence
- >LOC_Os04g32620.1
TATATAGGCAACTGGTTAGACTTTTTTTTCCCTTTGCCCATAATAAAGCCATCTCTCATAAAGCCATCGACCTCATCTCCTCCTCCTCCTCTCTCTGTCTCTCTCTGGCTGTTCTTATCAGTTTACGTTGTCCTCATCGTCGCCGGCGCCGCCCTCCAAAGCTTCATAGATAGCTCGATCGATCGATCCCGTCGTCGTCGATCGTCGTCGACGCAGCAGCCTCGCCGGCGGCCGGAGACTGGGGAGATCGATCGAGAGGGAGGGAGGTTGAGTAAGGTGGATCCACGGGTCAGGCATGGCAAGAGGCCCCTACCGGCCGGCGAGGAGGAGGAAGAAGACCAGCCACCGCCTCCGACACCACCAGCAAAGCAGGAGCAGCAGGACGAGGAGGAGCTCGCCGCGGTCGTCATCAGCGGCGGCGGAGCAGGAGCAGCTCCGGCGCCGGAGACGTACGCGCAGTACTACTACGCGGCGCGCGCCGACCACGACGCCTCCGCCATGGTCACCGCGCTAGCCCACGTCATCCGCGCCGCCCCCGACCTGCACCTCCCCCACCACCCATCCTCCTCCGCCTCCGCCGCCGCCCACCCGCAGCAGGCTTCTTCCTTCTACCCGACCGCCGCCGCCGCCGCCTCCTCGCCGTCCGACCAGCTCGCCGCCGCCGCCGCTGCCGAAGAGCAAGGGAGGAGGCGGCACTACAGAGGTGTTCGACAGCGGCCATGGGGGAAGTGGGCGGCGGAGATAAGGGACCCGAAGAAGGCGGCGAGGGTGTGGCTGGGGACGTTCGACACGGCGGAGGACGCCGCCATCGCCTACGACGAGGCGGCGCTCCGGTTCAAGGGGACCAAGGCCAAGCTCAACTTCCCCGAGCGCGTCCAGGGCCGCACCGACCTCGGCTTCCTCGTCACCCGTGGCATCCCCCCCGCCGCCACCCACGGTGGCGGCTACTACCCCTCGTCGTCGCCGGCGGCGGGGGCATGCCCCCCGCCGCGGCAGCAGCAGACGGTCGTGCCGTACCCGGACCTCATGCGGTACGCGCAGCTGCTGCAGGGCGGCGTCGGCGGCAGTTACATGCCGTTCGGCGGCGCCGCGACGATGTCGTCGTCGACGGTGTCGTCCTCGTCGGCGCCGCAGATACTCGACTTCTCGACGCAGCAGCTCATCCGGGCCGGCCCGCCGTCACCAATGCCATCGTCGGGCTCCGGCTCGGCGACCGCGGCGGCGTCGTCCACGACGTCGGCGTCGTCGCCCGGTGCATGGCCGTACGGCGGCTCGGAGCGCAAGAAGAAGGATTCGTCGTCGTGACGTCACTGGATGGATCGATCGATGCATGAATATGCCATGCATTTGAGAGGAAGAGGTCAAGTTTTTTTTTAAGCAATGGAAATGGATTACATCTTCGGCCTCGGCACGTGGACAGAAAGAGATCAAGTGATATGGATCGAGAAGAATGTTTAGCTGATCTGCTCTTTACTCTGCACGATCGAGGAGATGAAAATGCATGCAATATGCATGGAGGGGTAAGTGTTAAGTGGTTAATTGGTGATTTTTGCGGGTTAATTAATTACATGGACATTAATTTTTCTTTTGATCACCTTTTGTTTATTTTTTCTCTGAAAACTATCTTCTTTTGTTCATTTTTATTTTCTTGGAGGTACTTAATTAATTAGCTAGGTTAATTAGCTGGTGATTTTTAGTTTGTTGTTTCCCTCCTTTTGTTCAAGTGTGAACTAATTGTGCAAAATTAGTCCAAGTAAAGCAAAGAGAGAGGATTCTTATTTAATTATTGGTTAAACATTACCCTTTTTTAAGATGAAACATGATGTCTTTTTTTAAAAAAAAAATCTGAATGATTACCACCTCTTTGTCTACATCTTTGGATTGTAGTGGAAGCTTCTTCTTCACTGGTGATCTCTTTGGCAGACATTGGAAATGTCCAAAGGATACCATAAATGGATTTCAGTATACCTTTAGGAATAACATTTTGTTGTTTGGTAGCATGCATGATGGGTATCAATCAAATTTAACAACCTTTGGAAAAAAATAAGGGGGAATCTTCTCCATCAGCTTCTCTTATTAAAAGATTTGACTTTTTTGTTTAGATACTTTTATCATGGTTGCAATATTGGCATCTGTTCTATCGATTTATCATCTACTTAATCCTCCAATCTCATTCTCCGCTGTAATGATATTTAAGCATGTGTCATAGTGGTATGGATGCCTTTAATGCATGTAGCATATTTCAAACTCAGAACAAGATGTACTTTTAGAAAAATTAAAGTTGGAACCGTAACAAGAATCTAGAGAAAACCAGAAAAACGCTGCAGTTTGGTGAGGACATTTTACTTTTGCAATGATGCTTTGAGGTGATGAAGATTATCCACATACCTAATAATTATTCAGTTGTAAGAAATTAAAATAGCATGAAGTCATCCATTTCTTTTTTTTTCAAACGCTTTTGTACATAAGCAACATACATATTCCATCCCCAAAGCACTATAGCCACATATATATATATCTACATATGTCTTATATCATAAAAATGCTTATAGCTAGCTTCAGGTTTTTAATGACATGAACATGCATATGCCACGTAGATCTTGAGTTAATTATAGTCAGAGGTGTGGCGTAATGATCTCAAAGTTTCTAGTGTAAATCATAAAATATGTAGATTTGTGATTTGGTGCCATGTGTCGAGATTTATTCGATCAACTTGCCGGCATGTGATGATACGAAATAAGGAGAGAACCTCTATCATGCAAGTATCATCCCCATCCCGTGCATCTAGAAGCTAATATATATGGGCGATGCATGCACCAATTCAATGCCAAATCAAATGCATTCAACTGCATTTTTTTGTATTTACGCATTTTTATATACCAGTACATCTGTCCACCCATCTATCCAGCTACATATATGAGTGACAAAAATTACAACCACACTAGCATTTGTAGATACATGTGTGTCTTTTTCTCTATAGCTAGCTTGTGATGAGAACCAAAGAGACAGATCCATGTACAAGTGTCTTGATGTAACAGAGATCATCATTATACATGATTTGTTTCCATTAGGTACGTATCTCTTTCTTAATTTTCTCTACTATTGGTTGCATGAAACTTATTATATATATAACTATTCCAATTAACTAGGTTTATTTAATTTATACCTGTCTTTTAGTTTGTTCACACTGGGTGTGATTGATGGTGCTGTGGTGTGTTTGCCCTTTTTCTCTCCTTTACTTTCATGATGGGAACAAGTATCAAAATTTGACACGCACATTTTTTTTTTCTTTTTGCCAAATGCTACTGCTACCTGGCAGCTAGCGGCCGATCGAACTCGAGCAGAAAGTGCCAGCAGCAAGCAAGCTAGCTTTCGTGTTAGGCATGACTCATGCCTGTTTTGATTTTCAACACATATCATTTATCGAAACAGAGAAAAAAAAATTAAAGAAAACGAGTACCATGCATGCCTAATTATCTCTAGTACTAGCTTTTTAGGCGCACTATTGTATATTGTGTACTATGAAAGTATCGTGTTGTTGAGATGGATGGTCTTACACGTGTGCGCCATGCTTGATTACTTGTGTGTGATTGGCACCTAACTTGTGTGTGATCGATCGTGTCATCGTCCTTTGATAGAGAGGATAAGACGCTTCTCTCATCATCATCTCAGCTGGTCTAGCTCTCTGCCTAACTAGCCAAGAGTACCTAACTTAATTGGAGAATGTTCTCTCTTAATTGTATTGGCATGTTTCAAAGTCACTTGAGAATTAGGTTAGAGTGATTTTCAAGTGAAATTTAGAAACATATCGAAGTATATATGTATGCAACATTAGTTAATATAAGAAGTTTCAAGTGT
CDS Sequence
- >LOC_Os04g32620.1
ATGGTCACCGCGCTAGCCCACGTCATCCGCGCCGCCCCCGACCTGCACCTCCCCCACCACCCATCCTCCTCCGCCTCCGCCGCCGCCCACCCGCAGCAGGCTTCTTCCTTCTACCCGACCGCCGCCGCCGCCGCCTCCTCGCCGTCCGACCAGCTCGCCGCCGCCGCCGCTGCCGAAGAGCAAGGGAGGAGGCGGCACTACAGAGGTGTTCGACAGCGGCCATGGGGGAAGTGGGCGGCGGAGATAAGGGACCCGAAGAAGGCGGCGAGGGTGTGGCTGGGGACGTTCGACACGGCGGAGGACGCCGCCATCGCCTACGACGAGGCGGCGCTCCGGTTCAAGGGGACCAAGGCCAAGCTCAACTTCCCCGAGCGCGTCCAGGGCCGCACCGACCTCGGCTTCCTCGTCACCCGTGGCATCCCCCCCGCCGCCACCCACGGTGGCGGCTACTACCCCTCGTCGTCGCCGGCGGCGGGGGCATGCCCCCCGCCGCGGCAGCAGCAGACGGTCGTGCCGTACCCGGACCTCATGCGGTACGCGCAGCTGCTGCAGGGCGGCGTCGGCGGCAGTTACATGCCGTTCGGCGGCGCCGCGACGATGTCGTCGTCGACGGTGTCGTCCTCGTCGGCGCCGCAGATACTCGACTTCTCGACGCAGCAGCTCATCCGGGCCGGCCCGCCGTCACCAATGCCATCGTCGGGCTCCGGCTCGGCGACCGCGGCGGCGTCGTCCACGACGTCGGCGTCGTCGCCCGGTGCATGGCCGTACGGCGGCTCGGAGCGCAAGAAGAAGGATTCGTCGTCGTGA
Protein Sequence
- >LOC_Os04g32620.1
MVTALAHVIRAAPDLHLPHHPSSSASAAAHPQQASSFYPTAAAAASSPSDQLAAAAAAEEQGRRRHYRGVRQRPWGKWAAEIRDPKKAARVWLGTFDTAEDAAIAYDEAALRFKGTKAKLNFPERVQGRTDLGFLVTRGIPPAATHGGGYYPSSSPAAGACPPPRQQQTVVPYPDLMRYAQLLQGGVGGSYMPFGGAATMSSSTVSSSSAPQILDFSTQQLIRAGPPSPMPSSGSGSATAAASSTTSASSPGAWPYGGSERKKKDSSS*