Information report for RERJ1;OsOsbHLH006
Gene Details
|
|
Functional Descriptions
- Differential screening of a cDNA library constructed using poly(A)(+) RNA from suspension-cultured rice cells treated with jasmonic acid (JA) for 1/2h yielded a cDNA of a gene tentatively named RERJ1 that is upregulated in response to exogenous JA.
- Northern blot analysis indicated that the RERJ1 mRNA levels peaked at 1/2-1h after the addition of jasmonic acid and then decreased gradually.
- RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-loop-helix protein.
- We previously reported that RERJ1, a JA-inducible transcription factor in rice, is up-regulated by exposure to wounding and drought stress.
- RERJ1 expression was induced only at the region of injury after wounding, whereas expression was induced in the entire leaf after drought.
- According to JA measurements of stressed leaves, high accumulation of endogenous JA was only detected around the wound site in a rice leaves, whereas the drought treatment led to uniform accumulation of JA in the entire leaf, suggesting that RERJ1 will be a useful marker gene for studies on localization of JA in rice.
- Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice.
- The jasmonic acid (JA)-responsive gene RERJ1 isolated from suspension-cultured rice cells encodes a transcription factor with a basic helix-loop-helix motif.
- The phenotypes of transgenic rice plants overexpressing sense or antisense RERJ1 mRNA demonstrated that RERJ1 is involved in the growth inhibition of rice shoots caused by JA.
- It is also suggested that JA but not abscisic acid is involved in the up-regulation of RERJ1 expression caused by wounding and drought stress.
- These results suggest that RERJ1 plays a role as a transcriptional activator for regulating stress-inducible gene expression, with a strong correlation to JA accumulation in the stressed region.
- In this study, we found that RERJ1 is also expressed in rice plants in response to JA, and that its expression in rice leaves is up-regulated by exposure to wounding and drought stress.
- Involvement of the Basic Helix-Loop-Helix Transcription Factor RERJ1 in Wounding and Drought Stress Responses in Rice Plants.
- Our data suggest that OsbHLH6 controls disease resistance by dynamically regulating SA and JA signaling.
- Transgenic rice plants overexpressing OsbHLH6 display increased JA responsive gene expression and enhanced disease susceptibility to the pathogen.
- Nucleus-localized OsbHLH6 activates JA signaling and suppresses SA signaling; however, the SA regulator OsNPR1 (Nonexpressor of PR genes 1) sequesters OsbHLH6 in the cytosol to alleviate its effect.
- OsbHLH6 expression was upregulated during Magnaporthe oryzae infection.
Functional Keywords
- jasmonic , drought , transcription-factor , jasmonic-acid , growth , ja , leaf , shoot , resistance , disease , disease-resistance , JA , magnaporthe-oryzae , sa , SA , pathogen , JA-signaling
Literature and News
- RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-loop-helix protein . DOI: 10.1016/j.bbrc.2004.10.126 ; PMID: 15541369
- Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice . DOI: 10.1007/s00709-012-0400-z ; PMID: 22456953
- Involvement of the basic helix-loop-helix transcription factor RERJ1 in wounding and drought stress responses in rice plants . DOI: 10.1271/bbb.69.1042 ; PMID: 15914931
- A bHLH transcription activator regulates defense signaling by nucleo-cytosolic trafficking in rice . DOI: 10.1111/jipb.12922 ; PMID: 32129570
Gene Resources
- NCBI ID: AK242210
Homologs
- Brachypodium distachyon: Bradi5g05110.2.p
- Hordeum vulgare: MLOC_62844.2
- Panicum virgatum: Pavir.7KG065100.1.p, Pavir.7NG041300.1.p
- Setaria italica: Seita.7G045200.1.p
- Setaria viridis: Sevir.7G050300.1.p
- Triticum aestivum: TabHLH27-B1, Traes_2BL_8FED05903.1, TabHLH27-D1, Traes_2DL_DE3909A32.1, TabHLH27-A1, Traes_2AL_411B944D6.1
Sequences
cDNA Sequence
- >LOC_Os04g23550.2
AAGTGAGCTCGGAGTCGGAGAGAGACACAAGCCAACCTCGAACTCGAAGCAGAGCAAACGCGAAGCAGAGCAAGCGCCATGGACGCCGAGATGGCCATGGGCGAGAGCTTCGCCTACTACTGGGAGACGCAGCGCTACCTTGAGTCCGAGGAGCTCGACAGCATGTACCTGCCGACGCAGGATGACTCCAACTACGAGTCGAGCTCGCCGGACGGGTCGCACTCGTCGTCGGCGCCGGCGCCGGCGGCCGTGGGCGGGGACGCAGCCGCCGCGGTGGCGGGTAGCGGTGGCGGGATGACGACGATGATGATGGGCGGCGGCGGCGGCGGTGGCGACGACGCCGGTGGCGCGAACAAGAACATCCTCATGGAGCGCGACCGCCGCCGCAAGCTCAACGAGAAGCTCTACGCGCTCCGCAGTGTCGTGCCCAACATCACCAAGGTAAGGGAGAGAGGAGTAGAGGTGTTCGTCCATGGCGGCGACGGCGACATTGCTGACTGACTGCTCGTCGGTGGTTGGGCGCAGATGGACAAGGCGTCGATCATCAAGGACGCGATCGAGTACATCCAGCGCCTGCAGGCGGAGGAGCAGCAGATGCTCCGGGAGGTCGCCGCGCTCGAGTCCGCCGCCGCGGCGAGCGCCGCCCCGGCCGCGGCGAACCCGTTCGCCGGCCTCGGCGCCGACGAGGAACACGAGTACGGCCACCACCACCCCTCGTCGTCGTCGGAGCGGACGAAGAAGGTGAAGCGGGCGCTGTCCGTGTCGTCCATCAGCGACGCCCTGCTCGCCGCGGCGGCGCCGGCGCCGCCGGTGGAGATCCAGGAGCTGCGCGTGTCGGAGGTGGGCGACAGGGTGCTGGTGGTGAGCGTGACGTGCAGCAAGCGCCGCGACGCCATGGCCCGGGTGTGCCGCGCCCTCGAGGAGCTCCGCCTCCGCGTCATCACCGCCAACATCACCTCCGTCGCCGGCTGCCTCATGCACACCCTCTTCGTCGAGGTAGATCACATGGATAGTGTCCAAATGAAGCAGATGGTGGAGGCTGCTCTCTCCCAGCTTGTGGCGACCGGCAGTCCGTTGAGCTCCATGAGCTATTAGTTATGAGCTGGATAGATATATGGAGTCATGCGTTTTGGCCACCTACTTTACCTAGTCTCATTCGCAATAAAGCGCATTTTTATGTATGCTTGGTGATGTTGTTGTTGTCATTTTTGCGACACCCCACTACGTGATGGAGGTGGTTGTTTTCTTGCGAGGCAACGATTTAGTGGCTGGATGATAGATTTAAGTATTTTGGAAATCTTGAGTACGCTGACTTGTTTATGTAGCTATTAATATTGATGAATAATAAGGTGGTCCAGCTTAATGAAAACGAAAATGTGTGAATTACCTCTCTGAATGAA - >LOC_Os04g23550.1
AAGTGAGCTCGGAGTCGGAGAGAGACACAAGCCAACCTCGAACTCGAAGCAGAGCAAACGCGAAGCAGAGCAAGCGCCATGGACGCCGAGATGGCCATGGGCGAGAGCTTCGCCTACTACTGGGAGACGCAGCGCTACCTTGAGTCCGAGGAGCTCGACAGCATGTACCTGCCGACGCAGGATGACTCCAACTACGAGTCGAGCTCGCCGGACGGGTCGCACTCGTCGTCGGCGCCGGCGCCGGCGGCCGTGGGCGGGGACGCAGCCGCCGCGGTGGCGGGTAGCGGTGGCGGGATGACGACGATGATGATGGGCGGCGGCGGCGGCGGTGGCGACGACGCCGGTGGCGCGAACAAGAACATCCTCATGGAGCGCGACCGCCGCCGCAAGCTCAACGAGAAGCTCTACGCGCTCCGCAGTGTCGTGCCCAACATCACCAAGGTAAGGGAGAGAGGAGTAGAGGTGTTCGTCCATGGCGGCGACGGCGACATTGCTGACTGACTGCTCGTCGGTGGTTGGGCGCAGATGGACAAGGCGTCGATCATCAAGGACGCGATCGAGTACATCCAGCGCCTGCAGGCGGAGGAGCAGCAGATGCTCCGGGAGGTCGCCGCGCTCGAGTCCGCCGCCGCGGCGAGCGCCGCCCCGGCCGCGGCGAACCCGTTCGCCGGCCTCGGCGCCGACGAGGAACACGAGTACGGCCACCACCACCCCTCGTCGTCGTCGGAGCGGACGAAGAAGGTGAAGCGGGCGCTGTCCGTGTCGTCCATCAGCGACGCCCTGCTCGCCGCGGCGGCGCCGGCGCCGCCGGTGGAGATCCAGGAGCTGCGCGTGTCGGAGGTGGGCGACAGGGTGCTGGTGGTGAGCGTGACGTGCAGCAAGCGCCGCGACGCCATGGCCCGGGTGTGCCGCGCCCTCGAGGAGCTCCGCCTCCGCGTCATCACCGCCAACATCACCTCCGTCGCCGGCTGCCTCATGCACACCCTCTTCGTCGAGGTAGATCACATGGATAGTGTCCAAATGAAGCAGATGGTGGAGGCTGCTCTCTCCCAGCTTGTGGCGACCGGCAGTCCGTTGAGCTCCATGAGCTATTAGTTATGAGCTGGATAGATATATGGAGTCATGCGTTTTGGCCACCTACTTTACCTAGTCTCATTCGCAATAAAGCGCATTTTTATGTATGCTTGGTGATGTTGTTGTTGTCATTTTTGCGACACCCCACTACGTGATGGAGGTGGTTGTTTTCTTGCGAGGCAACGATTTAGTGGCTGGATGATAGATTTAAGTATTTTGGAAATCTTGAGTACGCTGACTTGTTTATGTAGCTATTAATATTGATGAATAATAAGGTGGTCCAGCTTAATGAAAACGAAAATGTGTGAATTACCTCTCTGAATGAA
CDS Sequence
- >LOC_Os04g23550.2
ATGGACAAGGCGTCGATCATCAAGGACGCGATCGAGTACATCCAGCGCCTGCAGGCGGAGGAGCAGCAGATGCTCCGGGAGGTCGCCGCGCTCGAGTCCGCCGCCGCGGCGAGCGCCGCCCCGGCCGCGGCGAACCCGTTCGCCGGCCTCGGCGCCGACGAGGAACACGAGTACGGCCACCACCACCCCTCGTCGTCGTCGGAGCGGACGAAGAAGGTGAAGCGGGCGCTGTCCGTGTCGTCCATCAGCGACGCCCTGCTCGCCGCGGCGGCGCCGGCGCCGCCGGTGGAGATCCAGGAGCTGCGCGTGTCGGAGGTGGGCGACAGGGTGCTGGTGGTGAGCGTGACGTGCAGCAAGCGCCGCGACGCCATGGCCCGGGTGTGCCGCGCCCTCGAGGAGCTCCGCCTCCGCGTCATCACCGCCAACATCACCTCCGTCGCCGGCTGCCTCATGCACACCCTCTTCGTCGAGGTAGATCACATGGATAGTGTCCAAATGAAGCAGATGGTGGAGGCTGCTCTCTCCCAGCTTGTGGCGACCGGCAGTCCGTTGAGCTCCATGAGCTATTAG - >LOC_Os04g23550.1
ATGGACAAGGCGTCGATCATCAAGGACGCGATCGAGTACATCCAGCGCCTGCAGGCGGAGGAGCAGCAGATGCTCCGGGAGGTCGCCGCGCTCGAGTCCGCCGCCGCGGCGAGCGCCGCCCCGGCCGCGGCGAACCCGTTCGCCGGCCTCGGCGCCGACGAGGAACACGAGTACGGCCACCACCACCCCTCGTCGTCGTCGGAGCGGACGAAGAAGGTGAAGCGGGCGCTGTCCGTGTCGTCCATCAGCGACGCCCTGCTCGCCGCGGCGGCGCCGGCGCCGCCGGTGGAGATCCAGGAGCTGCGCGTGTCGGAGGTGGGCGACAGGGTGCTGGTGGTGAGCGTGACGTGCAGCAAGCGCCGCGACGCCATGGCCCGGGTGTGCCGCGCCCTCGAGGAGCTCCGCCTCCGCGTCATCACCGCCAACATCACCTCCGTCGCCGGCTGCCTCATGCACACCCTCTTCGTCGAGGTAGATCACATGGATAGTGTCCAAATGAAGCAGATGGTGGAGGCTGCTCTCTCCCAGCTTGTGGCGACCGGCAGTCCGTTGAGCTCCATGAGCTATTAG
Protein Sequence
- >LOC_Os04g23550.2
MDKASIIKDAIEYIQRLQAEEQQMLREVAALESAAAASAAPAAANPFAGLGADEEHEYGHHHPSSSSERTKKVKRALSVSSISDALLAAAAPAPPVEIQELRVSEVGDRVLVVSVTCSKRRDAMARVCRALEELRLRVITANITSVAGCLMHTLFVEVDHMDSVQMKQMVEAALSQLVATGSPLSSMSY* - >LOC_Os04g23550.1
MDKASIIKDAIEYIQRLQAEEQQMLREVAALESAAAASAAPAAANPFAGLGADEEHEYGHHHPSSSSERTKKVKRALSVSSISDALLAAAAPAPPVEIQELRVSEVGDRVLVVSVTCSKRRDAMARVCRALEELRLRVITANITSVAGCLMHTLFVEVDHMDSVQMKQMVEAALSQLVATGSPLSSMSY*