Information report for OsWRKY51
Gene Details
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Functional Descriptions
- To address the cross-talk of GA and ABA signaling, we studied two rice WRKY genes (OsWRKY51 and OsWRKY71) that are ABA-inducible and GA-repressible in embryos and aleurone cells.
- In contrast, the third non-DNA-binding mutant enhances GA induction of Amy32b-GUS, by interfering with the binding of the wild-type OsWRKY71 or the OsWRKY71/OsWRKY51 repressing complex.
- Transgenic lines overexpressing OsWRKY51 showed growth retardation compared to WT plants.
- OsWRKY51 functions as a positive transcriptional regulator in defense signaling against Xanthomonas oryzae pv.
- To examine the role of OsWRKY51 in the defense response of rice, we generated OsWRKY51 overexpressing and underexpressing transgenic rice plants.
- Based on these results, we suggest that OsWRKY51 is a positive transcriptional regulator of defense signaling and has direct DNA binding ability to the promoter of OsPR10a, although it is reported to be a negative regulator in GA signaling.
- In contrast, transgenic lines underexpressing OsWRKY51 by RNA interference showed similar plant height with WT plants.
- Transient expression of OsWRKY51-green fluorescent protein fusion protein in rice protoplasts revealed that OsWRKY51 was localized in the nucleus.
- In the first transcriptional regulatory cascade, OsWRKY47 acted downstream of OsWRKY10 whereas OsWRKY51 acted upstream.
- Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of
-amylase genes.
Functional Keywords
- ga , ABA , growth , defense , defense-response , height , plant-height , nucleus , GA , transcriptional-regulator , transcription-factor
Literature and News
- Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells . DOI: 10.1111/j.1365-313X.2006.02694.x ; PMID: 16623886
- The WRKY Gene Family in Rice (Oryza sativa) . DOI: 10.1111/j.1744-7909.2007.00504.x
- Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells . DOI: 10.1104/pp.104.054312 ; PMID: 15618416
- OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae . DOI: 10.1007/s00299-016-2012-0 ; PMID: 27300023
- WRKY10 transcriptional regulatory cascades in rice are involved in basal defense and Xa1-mediated resistance . DOI: 10.1093/jxb/eraa135 ; PMID: 32227093
- IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways . DOI: 10.3390/ijms22126605 ; PMID: 34203082
Gene Resources
- UniProt: Q0JEE2
- EMBL: AK100954, AL731615, AP008210
- AlphaFoldDB: Q0JEE2
- EnsemblPlants: Os04t0287400-01
- Gramene: Os04t0287400-01
- KEGG: dosa:Os04g0287400
- Orthologous matrix: RKRCFEH
- InterPro: IPR003657, IPR018872, IPR036576
- PANTHER: PTHR31282
- SUPFAM: SSF118290
- PROSITE: PS50811
- Gene3D: 2.20.25.80
- OrthoDB: Q0JEE2
- SWISS-MODEL: Q0JEE2
- eggNOG: ENOG502QR3I
Sequences
cDNA Sequence
- >LOC_Os04g21950.1
ACTTCTTCTTCTTCTTCTTCCCCCCATTTCTCACTTACTCCGGCGATCACCGTGGTTGAGCGTCGGCGGCGGAGGAGGAGGCGATGATTACCATGGATCTGATGGGTGGGTACGGGCGGGTGGACGAGCAGGTGGCCATCCAGGAGGCGGCGGCGGCGGGGCTAAGGGGGATGGAGCATCTTATTCTGCAGCTGTCCCAGACTGGGACGAGCGAGAGGTCGCCGGCGCCGGCGCAGGAGCAGCAGCAACAGGTGGACTGCAGGGAGATCACGGACATGACGGTGTCCAAGTTCAAGAAGGTGATCTCCATGCTGAACCGCACCGGCCACGCGCGGTTCCGGCGGGGCCCGGTGGTGGCGCAGTCGTCGGGCCCGGCGGCGTCCGAGCCGGCGCCGGTGAGGTCGTCCCCGTCGGCGGTGTCGAGGCCCATGACGCTCGACTTCACCAAGGCGGCGTCCGGGTACGGCAAGGACGCCGGGTTCAGCGTCTCCGGCATCTCCGCCGCGAGCTCGTCCTTCCTCTCGTCGGTCACCGGCGACGGCAGCGTGTCCAACGGGCGCGGCGGCGGGTCATCCTCCCTGATGCTTCCCCCGCCGCCGGCGACCAGCTGCGGCAAGCCACCGCTGTCCTCCGCCGCCGCCGCCATGTCAGCCGGCGCAGGCCACAAGCGCAAGTGCCACGACCACGCGCACTCCGAGAACGTCGCCGGCGGCAAGTACGGATCCACCGGCGGCCGCTGCCACTGCTCCAAGCGCCGGAAGCATCGGGTGAAGAGGACGATCCGCGTGCCGGCGATAAGCTCGAAGGTGGCGGACATCCCCGCCGACGACTTCTCGTGGCGGAAGTACGGGCAGAAGCCCATCAAGGGCTCCCCCTTCCCACGAGGATACTACAAGTGTAGCACGCTGCGCGGTTGCCCGGCGAGGAAGCACGTGGAGCGCGACCCGACCGACCCGTCCATGCTCATCGTCACCTACGAGGGCGAGCACCGCCACTCCCCCTCCGCCGCCGGCCAGGACCACCCGCCGGCGCCGCCTCCGCCGCTGGCGCTGCCGCTCGCCTGAGATACCCCCGGTGAAGAAGAGTGAGAATTAGCTTGTCGGTCATCATAATTACCGTTAGAAATTATTTTTTTCGTTTTTCTCTCTTTTTTTTTCTTTTTTTTACTGCATCATTGTAGAACTAGCGTGTAAAACAATTTTAGGAGACTTTGAGACTGAGCTTTTACTGAAGAAAAAATAAAGCAGAACAAAAAAAAAAGAGAAGAAGGCGATCGAGACTATCAGTAGAGTAGAGAGTAGAAAAAGGCGTGCCGGAATTGAAGATTTTGACCGCGCATGCGAATTTGTTTATCAACTCGGGTTCAATCCCGCGGATTGGGGTCCGGTGGAGGTCAAAAGGAGGTGGGGCCCGGGGTGTTGAACGTTGCCCCCCGCCCACGGTCCTCGTGCGGGGCCCACGCGACAGTGACGGGGAGGGTCCCTCGTGTGTGTAAGTGTGGACGACGCGGTCAAAGAACGCGGCGTGGGGTGGGGCCCACCACGGCCTTGGCCTCCGGCGTCCGCCTCGCCCGCGCTGCCAACGTTGACTCGTGTCAGCCAGGCTGGAATCGGATTCGCTACGCCTCTCTGTCTCACTCTCTCTCTCTGTGTTTCGTATCATTTCGAATGGGACATTCGAGTTCGATATCTTGATGCGCTGTTTGGAGAAAGTTTTATAATAGAGGAAATTATCAACTTTAA
CDS Sequence
- >LOC_Os04g21950.1
ATGATTACCATGGATCTGATGGGTGGGTACGGGCGGGTGGACGAGCAGGTGGCCATCCAGGAGGCGGCGGCGGCGGGGCTAAGGGGGATGGAGCATCTTATTCTGCAGCTGTCCCAGACTGGGACGAGCGAGAGGTCGCCGGCGCCGGCGCAGGAGCAGCAGCAACAGGTGGACTGCAGGGAGATCACGGACATGACGGTGTCCAAGTTCAAGAAGGTGATCTCCATGCTGAACCGCACCGGCCACGCGCGGTTCCGGCGGGGCCCGGTGGTGGCGCAGTCGTCGGGCCCGGCGGCGTCCGAGCCGGCGCCGGTGAGGTCGTCCCCGTCGGCGGTGTCGAGGCCCATGACGCTCGACTTCACCAAGGCGGCGTCCGGGTACGGCAAGGACGCCGGGTTCAGCGTCTCCGGCATCTCCGCCGCGAGCTCGTCCTTCCTCTCGTCGGTCACCGGCGACGGCAGCGTGTCCAACGGGCGCGGCGGCGGGTCATCCTCCCTGATGCTTCCCCCGCCGCCGGCGACCAGCTGCGGCAAGCCACCGCTGTCCTCCGCCGCCGCCGCCATGTCAGCCGGCGCAGGCCACAAGCGCAAGTGCCACGACCACGCGCACTCCGAGAACGTCGCCGGCGGCAAGTACGGATCCACCGGCGGCCGCTGCCACTGCTCCAAGCGCCGGAAGCATCGGGTGAAGAGGACGATCCGCGTGCCGGCGATAAGCTCGAAGGTGGCGGACATCCCCGCCGACGACTTCTCGTGGCGGAAGTACGGGCAGAAGCCCATCAAGGGCTCCCCCTTCCCACGAGGATACTACAAGTGTAGCACGCTGCGCGGTTGCCCGGCGAGGAAGCACGTGGAGCGCGACCCGACCGACCCGTCCATGCTCATCGTCACCTACGAGGGCGAGCACCGCCACTCCCCCTCCGCCGCCGGCCAGGACCACCCGCCGGCGCCGCCTCCGCCGCTGGCGCTGCCGCTCGCCTGA
Protein Sequence
- >LOC_Os04g21950.1
MITMDLMGGYGRVDEQVAIQEAAAAGLRGMEHLILQLSQTGTSERSPAPAQEQQQQVDCREITDMTVSKFKKVISMLNRTGHARFRRGPVVAQSSGPAASEPAPVRSSPSAVSRPMTLDFTKAASGYGKDAGFSVSGISAASSSFLSSVTGDGSVSNGRGGGSSSLMLPPPPATSCGKPPLSSAAAAMSAGAGHKRKCHDHAHSENVAGGKYGSTGGRCHCSKRRKHRVKRTIRVPAISSKVADIPADDFSWRKYGQKPIKGSPFPRGYYKCSTLRGCPARKHVERDPTDPSMLIVTYEGEHRHSPSAAGQDHPPAPPPPLALPLA*