Gene Details:

• MSU gene ID: LOC_Os04g09900
• RAPdb gene ID: Os04g0178300
• Gene Symbol: OsCPS4 OsCyc1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Here, we apply reverse genetics, using knock-outs of the relevant diterpene synthases (copalyl diphosphate synthase 4 (OsCPS4) and kaurene synthase-like 4 (OsKSL4)), to demonstrate that rice momilactones are involved in allelopathy, including suppressing growth of the widespread rice paddy weed, barnyard grass (Echinochloa crus-galli).
• Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1.
• The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice.

Function-related keywords:

• growth ,  ga ,  gibberellin

Literature:

• Cloning and characterization of cDNAs encoding ent-copalyl diphosphate synthases in wheat: insight into the evolution of rice phytoalexin biosynthetic genes .  DOI:  10.1271/bbb.80781 ;  PMID:  19270400
• OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice .  DOI:  10.1074/jbc.M109.036871 ;  PMID:  19635799
• gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein (PBZ1) in response to cold stress and pathogen attack .  DOI:  10.1111/j.1365-3040.2005.01441.x ;  PMID:  17080612
• Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis .  DOI:  10.1111/j.1365-313X.2004.02175.x ;  PMID:  15341631
• Genetic evidence for natural product-mediated plant-plant allelopathy in rice (Oryza sativa) .  DOI:  10.1111/j.1469-8137.2011.04005.x ;  PMID:  22150231
• Inferring Roles in Defense from Metabolic Allocation of Rice Diterpenoids .  DOI:  10.1105/tpc.18.00205 ;  PMID:  29691314
• Identification of a small set of genes commonly regulated in rice roots in response to beneficial rhizobacteria .  DOI:  10.1007/s12298-020-00911-1 ;  PMID:  33424163
• A (conditional) role for labdane-related diterpenoid natural products in rice stomatal closure .  DOI:  10.1111/nph.17196 ;  PMID:  33458815
• Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice .  DOI:  10.3390/ijms22147656 ;  PMID:  34299275

Related News:

Gene Resources:

• NCBI ID: AY530101
• UniProt accessions:

Sequences: