Information report for OsHAD1
Gene Details
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Functional Descriptions
- Exogenous application of recombinant OsHAD1 protein in growth media supplemented with phytate, led to marked increase in growth and total P content of Pi deficient WT rice seedlings.
- Further, overexpression of OsHAD1 in rice resulted in enhanced phosphatase activity, biomass, total and soluble P content in Pi deficient transgenic seedlings treated with phytate as restricted Pi source.
- We conclude that besides dephosphorylation of cellular organic-P, OsHAD1 in coordination with kinases may regulate phosphorylation status of downstream targets to accomplish Pi homeostasis under limited Pi supply.
- Here, we identified and characterized a PHR2 regulated; novel low Pi responsive haloacid dehalogenase (HAD)-like hydrolase, OsHAD1.
- While, OsHAD1 is a functional HAD protein having both acid phosphatase and phytase activity; it showed little homology with other known low Pi responsive HAD superfamily members.
- To elucidate the underlying regulatory mechanisms of OsHAD1, we performed in-vitro pull down assays which revealed association of OsHAD1 with protein kinases.
Functional Keywords
- seedlings , growth , homeostasis , Pi , pi , Pi-homeostasis , protein-kinase
Literature and News
Gene Resources
- UniProt: Q75LI3
- EMBL: AP014959
- AlphaFoldDB: Q75LI3
- EnsemblPlants: Os03t0834050-01
- Gramene: Os03t0834050-01
- KEGG: osa:9266306
- Orthologous matrix: EAIPDLW
- InterPro: IPR006439, IPR010237, IPR023214
- PANTHER: PTHR12725, PTHR12725:SF117
- SUPFAM: SSF56784
- Gene3D: 3.40.50.1000
- OrthoDB: Q75LI3
- SWISS-MODEL: Q75LI3
- eggNOG: KOG3109
Sequences
cDNA Sequence
- >LOC_Os03g61829.1
AGGAGCCTTCAAGCCTTCATTGTTTTGAGGCTCTCAAATTGGTGTGATTTCATTTAGAAATCACCCTTTCAGCTTTGATCATCAAGAATGGAGGCTATAGTTGAAGGATCGAAGTATGAGTGCTTGCTGTTTGATTTGGATGATACCCTGTACCCCTTCAGCTCTGGCATCAATCTAGCTTGTCGCAAAAATATACAAGATTACATGCGTCGCCATCTGCGAATTGAAGAAAGCCAAATTGCAGATATGTGCCTGGAACTGTACAAGGAATATGGCACCACGATGGCTGGTCTTAAGGCATTAGGTTACGAGTTTGATAACGATGAATTTCATGCAAATGTCCATGGTACACTGCCATATGACAATCTGCATTTCGACCCTGTTTTGAGGACCCTTCTACTTTCTATTCCACAGAGAAAAATAATATTCACAAACTCTGATAAAGCTCATGCAGAGGAGGTTCTGTGCAGGGTGGGTATACAGGACTGCTTCGAAGGCATAATATGCTTCGAGACACTGAATCCACCAACCCCAACATGCCATGGTCTACACAAGCCCCTAAGTTCTATCTCTGATGAACTATCTTCTGATTTGGATGACCTGGATGAATCTGATGGCTTCAGACCTAAATCACCAATTCTCTGCAAACCCTCTATTGAAGCCATGGAAGCTGCGATTCGGATTGCGAATGTTGATCCTGAGAAAACAATCTTCTTTGATGACAGCGTTCGAAACATAGCGTCAGGAAAAGCTGCAGGCTTTCACACTGTGATTGTTGGGAGGCCAACGCTGGTTCCAGGTGCTGATCACGCTCTTGAAAGCATTCATAACATCAAGGAAGCATTGCCCGAGATATGGGACGGTTGGTCTGAATCTGATGTTGTTCTTGCTTCCACTGCTTCTGAGACAACGGTTATTGCTTGATTGTGTCATACTTTGCCCCATGGATATTATATTGTGCAAAAATGAATGTTTGATGAAAGTATATCAAAGATTGCTATTCATGGGTGCAAACAGAAGACACATGCTGACATGCATATAGTAGTAGGTAAGAGATTATGGATAAAAGAATTCCAGTATTGATGGAATTGTATCAATTGTACTTGGATAGATTAGTGAGAACTTTGAGAAGTAAATGTAGTGGATGGCTTGCACAAGAAAATGCTCTTGGGTTGCCATTTATAATGAGGAGCAACGGAATATCTTTCAAGAGGCTCTAATTATGCAACATCTTGTTTACAGGTGTTCCAAAAATGCATACATGATACATCGTGTTCCTGGAACT
CDS Sequence
- >LOC_Os03g61829.1
ATGGAGGCTATAGTTGAAGGATCGAAGTATGAGTGCTTGCTGTTTGATTTGGATGATACCCTGTACCCCTTCAGCTCTGGCATCAATCTAGCTTGTCGCAAAAATATACAAGATTACATGCGTCGCCATCTGCGAATTGAAGAAAGCCAAATTGCAGATATGTGCCTGGAACTGTACAAGGAATATGGCACCACGATGGCTGGTCTTAAGGCATTAGGTTACGAGTTTGATAACGATGAATTTCATGCAAATGTCCATGGTACACTGCCATATGACAATCTGCATTTCGACCCTGTTTTGAGGACCCTTCTACTTTCTATTCCACAGAGAAAAATAATATTCACAAACTCTGATAAAGCTCATGCAGAGGAGGTTCTGTGCAGGGTGGGTATACAGGACTGCTTCGAAGGCATAATATGCTTCGAGACACTGAATCCACCAACCCCAACATGCCATGGTCTACACAAGCCCCTAAGTTCTATCTCTGATGAACTATCTTCTGATTTGGATGACCTGGATGAATCTGATGGCTTCAGACCTAAATCACCAATTCTCTGCAAACCCTCTATTGAAGCCATGGAAGCTGCGATTCGGATTGCGAATGTTGATCCTGAGAAAACAATCTTCTTTGATGACAGCGTTCGAAACATAGCGTCAGGAAAAGCTGCAGGCTTTCACACTGTGATTGTTGGGAGGCCAACGCTGGTTCCAGGTGCTGATCACGCTCTTGAAAGCATTCATAACATCAAGGAAGCATTGCCCGAGATATGGGACGGTTGGTCTGAATCTGATGTTGTTCTTGCTTCCACTGCTTCTGAGACAACGGTTATTGCTTGA
Protein Sequence
- >LOC_Os03g61829.1
MEAIVEGSKYECLLFDLDDTLYPFSSGINLACRKNIQDYMRRHLRIEESQIADMCLELYKEYGTTMAGLKALGYEFDNDEFHANVHGTLPYDNLHFDPVLRTLLLSIPQRKIIFTNSDKAHAEEVLCRVGIQDCFEGIICFETLNPPTPTCHGLHKPLSSISDELSSDLDDLDESDGFRPKSPILCKPSIEAMEAAIRIANVDPEKTIFFDDSVRNIASGKAAGFHTVIVGRPTLVPGADHALESIHNIKEALPEIWDGWSESDVVLASTASETTVIA*