Gene Details:
- MSU gene ID: LOC_Os03g60370
- RAPdb gene ID: Os03g0818100
- Gene Symbol: OsPHY2
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Prokaryotic expressed OsPHY2 has a strong function on degradation of phytate, with optimal temperature of 47°C and optimal pH of 3.
- Under the guidance of patatin signal peptide (PSP) of potato, the OsPHY2 expressed in tobacco could be secreted by the rhizosphere and hydrolyze the phytate into Pi, leading to a significantly more inorganic phosphate (Pi) accumulated and growth improved in plants under the condition using phytate as the sole phosphorus source.
- During the seed germination, the transcripts of OsPHY2 were much more detected in the germinated seeds than in the primary roots and the leaves.
- Therefore, as one HAP type of the phytase genes, OsPHY2 plays a critical role on the degradation of the phytins during the seed germination and has a potential application on the generation of elite crop germplasms with high use of efficiency of phytate and a large quantity of organic phosphate compounds in the arable land.
- OsPHY2 contains HP_HAP_like domain, a 24-aa signal peptide at the N-terminal and was targeted to the cytoplasm membrane after ER sorted.
Function-related keywords:
- temperature , growth , seed , seed-germination , cytoplasm , primary-root , phosphate , phosphorus
Literature:
- Molecular characterization and functional analysis of OsPHY2, a phytase gene classified in histidine acid phosphatase type in rice Oryza sativa L . DOI:
Related News:
Gene Resources:
- NCBI ID: NM_001058240
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os03g60370.1
GTCTCTTCCCACTTCGCACCCACACAAGTAAAGCTCCCAAAATCAAATTAAATTAAAGAGAAAAAAAAGAAGAAGAGAAGGGCAAATCGATTCGCCATGGCTGCTCCCCGCACGCCTCTCCCCCTCGTCCTCCTCCTCGTCTCCGCCGCGCTCCTCGCGGCGGCTCCCCTCTCGCCGGCGGCCGAGACCGGTGCCGCCGCGTTCGACGTCCGCCGCCACCTCTCCACCGTCACCAGGTACGATGTGGCGAGGGGATCCAATAGTGTGTCCTCCGCGCCGTCTATGTCGGATGAGTGCCGCGTGATCCACCTCAATCTCGTGGCAAGACATGGGACTCGCGCACCTACCAAAAAGAGAATCAAAGAGCTGGATAGACTGGCGGTTCGGTTGAAGGCTCTTATCGATGAAGCAAAACAAGGGCCTGAAAGTGACTCCCTGAAAAAAATTCCTTCATGGATGAAAGGGTGGGAGTCACCCTGGAAAGGTAGGGTGAAAGGTGGTGAGCTGGTCAGTGAAGGGGAGGAAGAGCTATACAACCTTGCTATCAGAGTCAAGGAGAGGTTTCAAGGCCTATTTGATGAGGAATATCACCCTGATGTGTATTCAATAAGAGCAACTCAGGTTCCTCGGGCATCAGCTAGTGCAGTAGCATTTGGTTTGGGTCTACTTTCTGGGAAAGGGAAGCTTGGACCTGTGAAAAACCGTGCCTTTTCTGTTCTGAGTGAGAGTCGTGCAAGTGATATTTGTCTGCGATTCTTTGATAGCTGTGAAACATACAAGGACTACAGGAAAAGAAAGGAGCCTGATGTTGAAAAGCAAAAGGAACCAATTTTAGAACACGTCACATCGGCATTAGTTAACCGTTATCATCTCAATTTTACACCAAAAGATGTTTCTTCCCTCTGGTTCCTTTGCAAGCAGGAAGCATCTTTAATGAATATAACCAATCAAGCTTGTCAACTTTTTAATGAAGCTGAGGTTTATTTTCTAGAGTGGACAGATGATCTGGAGGGCTTTGTGCTAAAAGGTTATGGTGAGTCAATAAACTATCGGATGGGACTGCCATTGCTCAAGGACGTTGTCCAGTCAATGGAAGAAGCAATCGTTGCTAAAGAAGAAAACCACCCTGATGGTACATATGAGAAGGCAAGGCTCCGATTTGCACATGCTGAAACTGTTGTCCCTTTCTCATGTCTTCTTGGTCTTTTTCTTGAAGGATCAGATTTTGCGAAGATACAACGGGAGGAATCATTGGACATACCTCCTGTGCCACCACAGGGAAGAAATTGGAAGGGCAGTGTTGTTGCACCTTTTGCTGGTAACAATATGTTGGCTTTGTACCAGTGCCCAGGAAAAACTGATGGTGGTAAGATTTCTCGGGATCAGAAGAGCTCATACTTCGTGCAGGTTATACACAATGAAGCTCCAGTTTCAATGCCGGGATGCGGGAACAAAGATTTCTGCCCATTTGAAGAGTTCAAGGAGAAGATAGTTGAACCCCACCTGAAGCATGACTACGACGCCCTATGCAAGATAAGGCCGGTGGCAAGAGAGGAGCCTTCCTCCTTCAGTTCCAGGATGTCCAATTTCTTCCTAGGTTTGTTCTCGCAGAAAGGATACCGTGTTAGTGCTCAGGATGTGAAGTCGGAGCTGTAGGTTTATACTAGGCAGATGGTGCATCTTCTACCTTTCGCACTAAGCTGCTACTATCCATCTACCATGGATAACCAAGGGACTGACTCGTGGACTAAACTACGGTGTTTCTGTACTTGCAACCCTCAGTTTGATGAGCGAGTCCAATGTGTAACAACACGCTATCACTGGTGCCCCTCTTCTTTCAGATAGAAGACGTTTGTTGATCAATGTAATCTTTGTTTTAATCTGGACTTTCTCTAGTGCTCAACTGCTGACTCTGTTGAGATCAAACTGAAAGTACTAGCACACGGTTGTGATTGTGGCCCTTGTGGTAGGTTGAGATCATGTACTACTACTTGGACAATCCGTTTCAAGCTTTCAGCTCAATCAATCCAGATTTGGGAGATCCCCAATTCGAGATGAATCATGACAAA
CDS Sequence
- >LOC_Os03g60370.1
ATGGCTGCTCCCCGCACGCCTCTCCCCCTCGTCCTCCTCCTCGTCTCCGCCGCGCTCCTCGCGGCGGCTCCCCTCTCGCCGGCGGCCGAGACCGGTGCCGCCGCGTTCGACGTCCGCCGCCACCTCTCCACCGTCACCAGGTACGATGTGGCGAGGGGATCCAATAGTGTGTCCTCCGCGCCGTCTATGTCGGATGAGTGCCGCGTGATCCACCTCAATCTCGTGGCAAGACATGGGACTCGCGCACCTACCAAAAAGAGAATCAAAGAGCTGGATAGACTGGCGGTTCGGTTGAAGGCTCTTATCGATGAAGCAAAACAAGGGCCTGAAAGTGACTCCCTGAAAAAAATTCCTTCATGGATGAAAGGGTGGGAGTCACCCTGGAAAGGTAGGGTGAAAGGTGGTGAGCTGGTCAGTGAAGGGGAGGAAGAGCTATACAACCTTGCTATCAGAGTCAAGGAGAGGTTTCAAGGCCTATTTGATGAGGAATATCACCCTGATGTGTATTCAATAAGAGCAACTCAGGTTCCTCGGGCATCAGCTAGTGCAGTAGCATTTGGTTTGGGTCTACTTTCTGGGAAAGGGAAGCTTGGACCTGTGAAAAACCGTGCCTTTTCTGTTCTGAGTGAGAGTCGTGCAAGTGATATTTGTCTGCGATTCTTTGATAGCTGTGAAACATACAAGGACTACAGGAAAAGAAAGGAGCCTGATGTTGAAAAGCAAAAGGAACCAATTTTAGAACACGTCACATCGGCATTAGTTAACCGTTATCATCTCAATTTTACACCAAAAGATGTTTCTTCCCTCTGGTTCCTTTGCAAGCAGGAAGCATCTTTAATGAATATAACCAATCAAGCTTGTCAACTTTTTAATGAAGCTGAGGTTTATTTTCTAGAGTGGACAGATGATCTGGAGGGCTTTGTGCTAAAAGGTTATGGTGAGTCAATAAACTATCGGATGGGACTGCCATTGCTCAAGGACGTTGTCCAGTCAATGGAAGAAGCAATCGTTGCTAAAGAAGAAAACCACCCTGATGGTACATATGAGAAGGCAAGGCTCCGATTTGCACATGCTGAAACTGTTGTCCCTTTCTCATGTCTTCTTGGTCTTTTTCTTGAAGGATCAGATTTTGCGAAGATACAACGGGAGGAATCATTGGACATACCTCCTGTGCCACCACAGGGAAGAAATTGGAAGGGCAGTGTTGTTGCACCTTTTGCTGGTAACAATATGTTGGCTTTGTACCAGTGCCCAGGAAAAACTGATGGTGGTAAGATTTCTCGGGATCAGAAGAGCTCATACTTCGTGCAGGTTATACACAATGAAGCTCCAGTTTCAATGCCGGGATGCGGGAACAAAGATTTCTGCCCATTTGAAGAGTTCAAGGAGAAGATAGTTGAACCCCACCTGAAGCATGACTACGACGCCCTATGCAAGATAAGGCCGGTGGCAAGAGAGGAGCCTTCCTCCTTCAGTTCCAGGATGTCCAATTTCTTCCTAGGTTTGTTCTCGCAGAAAGGATACCGTGTTAGTGCTCAGGATGTGAAGTCGGAGCTGTAG
Protein Sequence
- >LOC_Os03g60370.1
MAAPRTPLPLVLLLVSAALLAAAPLSPAAETGAAAFDVRRHLSTVTRYDVARGSNSVSSAPSMSDECRVIHLNLVARHGTRAPTKKRIKELDRLAVRLKALIDEAKQGPESDSLKKIPSWMKGWESPWKGRVKGGELVSEGEEELYNLAIRVKERFQGLFDEEYHPDVYSIRATQVPRASASAVAFGLGLLSGKGKLGPVKNRAFSVLSESRASDICLRFFDSCETYKDYRKRKEPDVEKQKEPILEHVTSALVNRYHLNFTPKDVSSLWFLCKQEASLMNITNQACQLFNEAEVYFLEWTDDLEGFVLKGYGESINYRMGLPLLKDVVQSMEEAIVAKEENHPDGTYEKARLRFAHAETVVPFSCLLGLFLEGSDFAKIQREESLDIPPVPPQGRNWKGSVVAPFAGNNMLALYQCPGKTDGGKISRDQKSSYFVQVIHNEAPVSMPGCGNKDFCPFEEFKEKIVEPHLKHDYDALCKIRPVAREEPSSFSSRMSNFFLGLFSQKGYRVSAQDVKSEL*