Gene Details:

Functional Descriptions:

  • Prokaryotic expressed OsPHY2 has a strong function on degradation of phytate, with optimal temperature of 47°C and optimal pH of 3.
  • Under the guidance of patatin signal peptide (PSP) of potato, the OsPHY2 expressed in tobacco could be secreted by the rhizosphere and hydrolyze the phytate into Pi, leading to a significantly more inorganic phosphate (Pi) accumulated and growth improved in plants under the condition using phytate as the sole phosphorus source.
  • During the seed germination, the transcripts of OsPHY2 were much more detected in the germinated seeds than in the primary roots and the leaves.
  • Therefore, as one HAP type of the phytase genes, OsPHY2 plays a critical role on the degradation of the phytins during the seed germination and has a potential application on the generation of elite crop germplasms with high use of efficiency of phytate and a large quantity of organic phosphate compounds in the arable land.
  • OsPHY2 contains HP_HAP_like domain, a 24-aa signal peptide at the N-terminal and was targeted to the cytoplasm membrane after ER sorted.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g60370.1
    GTCTCTTCCCACTTCGCACCCACACAAGTAAAGCTCCCAAAATCAAATTAAATTAAAGAGAAAAAAAAGAAGAAGAGAAGGGCAAATCGATTCGCCATGGCTGCTCCCCGCACGCCTCTCCCCCTCGTCCTCCTCCTCGTCTCCGCCGCGCTCCTCGCGGCGGCTCCCCTCTCGCCGGCGGCCGAGACCGGTGCCGCCGCGTTCGACGTCCGCCGCCACCTCTCCACCGTCACCAGGTACGATGTGGCGAGGGGATCCAATAGTGTGTCCTCCGCGCCGTCTATGTCGGATGAGTGCCGCGTGATCCACCTCAATCTCGTGGCAAGACATGGGACTCGCGCACCTACCAAAAAGAGAATCAAAGAGCTGGATAGACTGGCGGTTCGGTTGAAGGCTCTTATCGATGAAGCAAAACAAGGGCCTGAAAGTGACTCCCTGAAAAAAATTCCTTCATGGATGAAAGGGTGGGAGTCACCCTGGAAAGGTAGGGTGAAAGGTGGTGAGCTGGTCAGTGAAGGGGAGGAAGAGCTATACAACCTTGCTATCAGAGTCAAGGAGAGGTTTCAAGGCCTATTTGATGAGGAATATCACCCTGATGTGTATTCAATAAGAGCAACTCAGGTTCCTCGGGCATCAGCTAGTGCAGTAGCATTTGGTTTGGGTCTACTTTCTGGGAAAGGGAAGCTTGGACCTGTGAAAAACCGTGCCTTTTCTGTTCTGAGTGAGAGTCGTGCAAGTGATATTTGTCTGCGATTCTTTGATAGCTGTGAAACATACAAGGACTACAGGAAAAGAAAGGAGCCTGATGTTGAAAAGCAAAAGGAACCAATTTTAGAACACGTCACATCGGCATTAGTTAACCGTTATCATCTCAATTTTACACCAAAAGATGTTTCTTCCCTCTGGTTCCTTTGCAAGCAGGAAGCATCTTTAATGAATATAACCAATCAAGCTTGTCAACTTTTTAATGAAGCTGAGGTTTATTTTCTAGAGTGGACAGATGATCTGGAGGGCTTTGTGCTAAAAGGTTATGGTGAGTCAATAAACTATCGGATGGGACTGCCATTGCTCAAGGACGTTGTCCAGTCAATGGAAGAAGCAATCGTTGCTAAAGAAGAAAACCACCCTGATGGTACATATGAGAAGGCAAGGCTCCGATTTGCACATGCTGAAACTGTTGTCCCTTTCTCATGTCTTCTTGGTCTTTTTCTTGAAGGATCAGATTTTGCGAAGATACAACGGGAGGAATCATTGGACATACCTCCTGTGCCACCACAGGGAAGAAATTGGAAGGGCAGTGTTGTTGCACCTTTTGCTGGTAACAATATGTTGGCTTTGTACCAGTGCCCAGGAAAAACTGATGGTGGTAAGATTTCTCGGGATCAGAAGAGCTCATACTTCGTGCAGGTTATACACAATGAAGCTCCAGTTTCAATGCCGGGATGCGGGAACAAAGATTTCTGCCCATTTGAAGAGTTCAAGGAGAAGATAGTTGAACCCCACCTGAAGCATGACTACGACGCCCTATGCAAGATAAGGCCGGTGGCAAGAGAGGAGCCTTCCTCCTTCAGTTCCAGGATGTCCAATTTCTTCCTAGGTTTGTTCTCGCAGAAAGGATACCGTGTTAGTGCTCAGGATGTGAAGTCGGAGCTGTAGGTTTATACTAGGCAGATGGTGCATCTTCTACCTTTCGCACTAAGCTGCTACTATCCATCTACCATGGATAACCAAGGGACTGACTCGTGGACTAAACTACGGTGTTTCTGTACTTGCAACCCTCAGTTTGATGAGCGAGTCCAATGTGTAACAACACGCTATCACTGGTGCCCCTCTTCTTTCAGATAGAAGACGTTTGTTGATCAATGTAATCTTTGTTTTAATCTGGACTTTCTCTAGTGCTCAACTGCTGACTCTGTTGAGATCAAACTGAAAGTACTAGCACACGGTTGTGATTGTGGCCCTTGTGGTAGGTTGAGATCATGTACTACTACTTGGACAATCCGTTTCAAGCTTTCAGCTCAATCAATCCAGATTTGGGAGATCCCCAATTCGAGATGAATCATGACAAA
CDS Sequence
  • >LOC_Os03g60370.1
    ATGGCTGCTCCCCGCACGCCTCTCCCCCTCGTCCTCCTCCTCGTCTCCGCCGCGCTCCTCGCGGCGGCTCCCCTCTCGCCGGCGGCCGAGACCGGTGCCGCCGCGTTCGACGTCCGCCGCCACCTCTCCACCGTCACCAGGTACGATGTGGCGAGGGGATCCAATAGTGTGTCCTCCGCGCCGTCTATGTCGGATGAGTGCCGCGTGATCCACCTCAATCTCGTGGCAAGACATGGGACTCGCGCACCTACCAAAAAGAGAATCAAAGAGCTGGATAGACTGGCGGTTCGGTTGAAGGCTCTTATCGATGAAGCAAAACAAGGGCCTGAAAGTGACTCCCTGAAAAAAATTCCTTCATGGATGAAAGGGTGGGAGTCACCCTGGAAAGGTAGGGTGAAAGGTGGTGAGCTGGTCAGTGAAGGGGAGGAAGAGCTATACAACCTTGCTATCAGAGTCAAGGAGAGGTTTCAAGGCCTATTTGATGAGGAATATCACCCTGATGTGTATTCAATAAGAGCAACTCAGGTTCCTCGGGCATCAGCTAGTGCAGTAGCATTTGGTTTGGGTCTACTTTCTGGGAAAGGGAAGCTTGGACCTGTGAAAAACCGTGCCTTTTCTGTTCTGAGTGAGAGTCGTGCAAGTGATATTTGTCTGCGATTCTTTGATAGCTGTGAAACATACAAGGACTACAGGAAAAGAAAGGAGCCTGATGTTGAAAAGCAAAAGGAACCAATTTTAGAACACGTCACATCGGCATTAGTTAACCGTTATCATCTCAATTTTACACCAAAAGATGTTTCTTCCCTCTGGTTCCTTTGCAAGCAGGAAGCATCTTTAATGAATATAACCAATCAAGCTTGTCAACTTTTTAATGAAGCTGAGGTTTATTTTCTAGAGTGGACAGATGATCTGGAGGGCTTTGTGCTAAAAGGTTATGGTGAGTCAATAAACTATCGGATGGGACTGCCATTGCTCAAGGACGTTGTCCAGTCAATGGAAGAAGCAATCGTTGCTAAAGAAGAAAACCACCCTGATGGTACATATGAGAAGGCAAGGCTCCGATTTGCACATGCTGAAACTGTTGTCCCTTTCTCATGTCTTCTTGGTCTTTTTCTTGAAGGATCAGATTTTGCGAAGATACAACGGGAGGAATCATTGGACATACCTCCTGTGCCACCACAGGGAAGAAATTGGAAGGGCAGTGTTGTTGCACCTTTTGCTGGTAACAATATGTTGGCTTTGTACCAGTGCCCAGGAAAAACTGATGGTGGTAAGATTTCTCGGGATCAGAAGAGCTCATACTTCGTGCAGGTTATACACAATGAAGCTCCAGTTTCAATGCCGGGATGCGGGAACAAAGATTTCTGCCCATTTGAAGAGTTCAAGGAGAAGATAGTTGAACCCCACCTGAAGCATGACTACGACGCCCTATGCAAGATAAGGCCGGTGGCAAGAGAGGAGCCTTCCTCCTTCAGTTCCAGGATGTCCAATTTCTTCCTAGGTTTGTTCTCGCAGAAAGGATACCGTGTTAGTGCTCAGGATGTGAAGTCGGAGCTGTAG
Protein Sequence
  • >LOC_Os03g60370.1
    MAAPRTPLPLVLLLVSAALLAAAPLSPAAETGAAAFDVRRHLSTVTRYDVARGSNSVSSAPSMSDECRVIHLNLVARHGTRAPTKKRIKELDRLAVRLKALIDEAKQGPESDSLKKIPSWMKGWESPWKGRVKGGELVSEGEEELYNLAIRVKERFQGLFDEEYHPDVYSIRATQVPRASASAVAFGLGLLSGKGKLGPVKNRAFSVLSESRASDICLRFFDSCETYKDYRKRKEPDVEKQKEPILEHVTSALVNRYHLNFTPKDVSSLWFLCKQEASLMNITNQACQLFNEAEVYFLEWTDDLEGFVLKGYGESINYRMGLPLLKDVVQSMEEAIVAKEENHPDGTYEKARLRFAHAETVVPFSCLLGLFLEGSDFAKIQREESLDIPPVPPQGRNWKGSVVAPFAGNNMLALYQCPGKTDGGKISRDQKSSYFVQVIHNEAPVSMPGCGNKDFCPFEEFKEKIVEPHLKHDYDALCKIRPVAREEPSSFSSRMSNFFLGLFSQKGYRVSAQDVKSEL*