Gene Details:

Functional Descriptions:

  • The rice U-box/ARM E3 ubiquitin ligase SPL11 negatively regulates programmed cell death (PCD) and disease resistance, and controls flowering time through interacting with the novel RNA/DNA binding KH domain protein SPIN1.
  • SPIN1 overexpression causes late flowering independently of daylength; expression analyses of flowering marker genes in these lines suggested that SPIN1 represses flowering by downregulating the flowering promoter gene Heading date3a (Hd3a) via Hd1-dependent mechanisms in short days and by targeting Hd1-independent factors in long days.
  • SPIN1, a K homology domain protein negatively regulated and ubiquitinated by the E3 ubiquitin ligase SPL11, is involved in flowering time control in rice.
  • Overexpression of SPIN1 causes late flowering in transgenic rice under short-day (SD) and long-day (LD) conditions.
  • In this study, we characterized the function of the RNA-binding and SPIN1-interacting 1 (RBS1) protein in flowering time regulation.
  • These results suggest that RBS1 is a new negative regulator of flowering time that itself is positively regulated by SPIN1 but negatively regulated by SPL11 in rice.
  • RBS1, an RNA Binding Protein, Interacts with SPIN1 and Is Involved in Flowering Time Control in Rice.
  • We demonstrate here that SPL11 also regulates flowering via interaction with SPIN1 (for SPL11-interacting protein1), a Signal Transduction and Activation of RNA family member.
  • Moreover, we show that coincidence of high accumulation of SPIN1 mRNA with the light in the morning and early evening is needed to repress flowering.
  • Our data are consistent with a model in which SPIN1 acts as a negative regulator of flowering that itself is negatively regulated by SPL11, possibly via ubiquitination.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g60110.1
    ATGTTTTCCATTCCATTGGGTTTGGTGTCGTGTTGTGAATGTGTTGAGAAAGAGTAGAGGAGAGAACCAAAAGGGTTTCCAGAGCGGCAGCGGCTGCCTGCTTAGCTGCTGCTGATGCTGCAGGCTGCACCAAAGCAGCGAGCCTTTTTTTACCTCTCCTACTATACCACCACCACCATCTCCTCTCCTCGGCTTCACTTCTCGCCTCTCCTTGTTGGCACGGGCGGCGATTGAGCCCGCGGCGGCGCCATGTCGGGGCTGTACAGCCCGGGGTTCTCGCCAGCGAGGAACCTCTCCCCCCAGATTAGGAGCAACCCGACGGATGTTGATAGTCAGTATCTGGCCGAGTTGCTCGCCGAGCATCAGAAGCTGGGGCCGTTCATGCAGGTGCTGCCCATATGCAGCAAGCTGCTGAGCCAAGAAATTATGCGGGTATCAAGCATTGTCCACAACCATGGATTTGGTGATTTTGACAGGCATCGGTTTAGAAGTCCTAGCCCTATGTCTTCGCCAAACCCAAGATCTAATCGCTCTGGAAATGGGTTTAGTCCTTGGAATGGGCTACACCAAGAGAGATTAGGTTTTCCTCAAGGAACCAGCATGGATTGGCAGGGAGCACCACCAAGCCCTAGTTCTCATGTTGTAAAAAAGATTCTGCGTTTGGATGTCCCAGTTGATTCTTATCCAAATTTCAATTTTGTGGGACGCATTCTAGGTCCTAGAGGTAACTCTCTAAAGCGGGTGGAAGCATCCACTGGCTGTCGTGTTTTCATTAGAGGGAAGGGTTCCATCAAAGATCCAGGGAAGGAGGACAAACTCCGTGGAAAGCCAGGCTACGAACATCTGAGTGACCCACTGCACATTTTGATAGAAGCTGAGTTCCCAGCCAGTATCATTGATGCAAGATTGAGACATGCACAGGAGGTTATAGAAGAACTGCTAAAACCAGTGGATGAATCACAGGATTTCTACAAAAGGCAACAGCTCAGGGAGCTGGCAATGCTAAACTCAACCCTGAGGGAGGACAGCCCCCATCCTGGGAGTGTCTCTCCATTCAGTAATGGCGGTATGAAACGCGCGAAAACGGGCCAGTAGAGAAGCAACTCTACCACCACCCATTTGAGGTGGTATCCAGGTGCCTTATGCCCTTAAAAAGGATGGCATTGGCACACCTTCAGGCATTGTTTGCCATGGGTGTGTGTGAAGGAAGCAATGCAATGCACCTTCACTAGATGGAGTTTGGTATCAGTCAGATTTTCGCCTTCAGGCAGGGGAAAACTTCAGGGACAATGACAATAGTCGCAGATCGTCCGGTCGATCCACCTTTGGTTTGTTGTAAGGTCTCAAGAACATTTGTTTTATTTGTTATTGACTGTTAACCCTAGTATGTGGTGCTATGGTAGGTTATCTCCATGACAAGCTAGAGAAGGCCTGGGATGTGAGCCTTTTTTTTTCTTCCATTTACCCCCCATTTAGGTGTTTAATTCACCTTGTATTATATCCTTTCACATTTATGGAGCTGTGAAAAATAAAAGAAGGATGAAGGGTCATCCTCTTTCTTCTGCCTTATAGTATGTCAGTATTTGGTTTAGCTGTAAAAATATTCGAGGTTATTCCTCCCTCCAATTTAAATTATTCTAGCTTTGTCCTAAATCAAATAAA
CDS Sequence
  • >LOC_Os03g60110.1
    ATGTCGGGGCTGTACAGCCCGGGGTTCTCGCCAGCGAGGAACCTCTCCCCCCAGATTAGGAGCAACCCGACGGATGTTGATAGTCAGTATCTGGCCGAGTTGCTCGCCGAGCATCAGAAGCTGGGGCCGTTCATGCAGGTGCTGCCCATATGCAGCAAGCTGCTGAGCCAAGAAATTATGCGGGTATCAAGCATTGTCCACAACCATGGATTTGGTGATTTTGACAGGCATCGGTTTAGAAGTCCTAGCCCTATGTCTTCGCCAAACCCAAGATCTAATCGCTCTGGAAATGGGTTTAGTCCTTGGAATGGGCTACACCAAGAGAGATTAGGTTTTCCTCAAGGAACCAGCATGGATTGGCAGGGAGCACCACCAAGCCCTAGTTCTCATGTTGTAAAAAAGATTCTGCGTTTGGATGTCCCAGTTGATTCTTATCCAAATTTCAATTTTGTGGGACGCATTCTAGGTCCTAGAGGTAACTCTCTAAAGCGGGTGGAAGCATCCACTGGCTGTCGTGTTTTCATTAGAGGGAAGGGTTCCATCAAAGATCCAGGGAAGGAGGACAAACTCCGTGGAAAGCCAGGCTACGAACATCTGAGTGACCCACTGCACATTTTGATAGAAGCTGAGTTCCCAGCCAGTATCATTGATGCAAGATTGAGACATGCACAGGAGGTTATAGAAGAACTGCTAAAACCAGTGGATGAATCACAGGATTTCTACAAAAGGCAACAGCTCAGGGAGCTGGCAATGCTAAACTCAACCCTGAGGGAGGACAGCCCCCATCCTGGGAGTGTCTCTCCATTCAGTAATGGCGGTATGAAACGCGCGAAAACGGGCCAGTAG
Protein Sequence
  • >LOC_Os03g60110.1
    MSGLYSPGFSPARNLSPQIRSNPTDVDSQYLAELLAEHQKLGPFMQVLPICSKLLSQEIMRVSSIVHNHGFGDFDRHRFRSPSPMSSPNPRSNRSGNGFSPWNGLHQERLGFPQGTSMDWQGAPPSPSSHVVKKILRLDVPVDSYPNFNFVGRILGPRGNSLKRVEASTGCRVFIRGKGSIKDPGKEDKLRGKPGYEHLSDPLHILIEAEFPASIIDARLRHAQEVIEELLKPVDESQDFYKRQQLRELAMLNSTLREDSPHPGSVSPFSNGGMKRAKTGQ*