Gene Details:

• MSU gene ID: LOC_Os03g59610
• RAPdb gene ID: Os03g0810800
• Gene Symbol: OsABA2
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Moreover, expression of OsABA2 in an Arabidopsis aba2 mutant rescued the aba2 mutant phenotypes, characterized by reduced growth, increased water loss, and germination in the presence of paclobutrazol, a gibberellin biosynthesis inhibitor or high concentration of glucose.
• Expression analysis of OsABA2 mRNA showed that the transcript level did not change in response to treatment with ABA or dehydration.
• These results indicate that OsABA2 is a rice XanDH that functions in ABA biosynthesis.
• Map-based cloning identified a G-to-A point mutation resulting in a D-to-N substitution at the amino acid position 110 of OsABA2 (LOC_Os03g59610) in lmm9150.
• Consistent with the function of OsABA2 in ABA biosynthesis, ABA level in the lmm9150 mutant was significantly reduced.
• We also found that OsABA2 has feedback regulation on OsMPK1 kinase activity.
• Finally, genetic analysis showed that OsABA2 can enhance the sensitivity of rice to ABA and the tolerance of rice to drought and salt stress.
• Further research reveals that OsMPK1 and OsABA2 coordinately regulate the biosynthesis of ABA, and phosphorylation of OsABA2 at Ser197 by OsMPK1 plays a crucial role in regulating the biosynthesis of ABA.
• Phosphorylation of OsABA2 at Ser197 by OsMPK1 regulates abscisic acid biosynthesis in rice.
• Here, by using yeast two-hybrid assay and firefly luciferase complementary imaging assay, we show that OsMPK1 physically interact with a short-chain dehydrogenase protein OsABA2.

Function-related keywords:

• gibberellin ,  ABA ,  gibberellin-biosynthesis ,  map-based-cloning ,  ABA-biosynthesis ,  Kinase ,  kinase ,  drought ,  salt ,  tolerance ,  salt-stress ,  stress ,  abscisic-acid ,  dehydrogenase ,  abscisic-acid-biosynthesis

Literature:

• Functional characterization of xanthoxin dehydrogenase in rice .  DOI:  10.1016/j.jplph.2014.05.003 ;  PMID:  25014258
• Mutation in Rice Abscisic Acid2 Results in Cell Death, Enhanced Disease-Resistance, Altered Seed Dormancy and Development .  DOI:  10.3389/fpls.2018.00405 ;  PMID:  29643863
• Phosphorylation of OsABA2 at Ser197 by OsMPK1 regulates abscisic acid biosynthesis in rice .  DOI:  10.1016/j.bbrc.2021.11.067 ;  PMID:  34826703

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: