Gene Details:

• MSU gene ID: LOC_Os03g58420
• RAPdb gene ID: Os03g0798500
• Gene Symbol: OsWRKY6
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Heterologous expression of OsWRKY6 enhanced disease resistance to pathogen.
• All together, OsWRKY6 functions as a positive transcriptional regulator of the plant defense response.
• Defense-related genes were constitutively expressed in Arabidopsis transgenic lines overexpressing OsWRKY6.
• Heterologous expression of OsWRKY6 gene in Arabidopsis activates the expression of defense related genes and enhances resistance to pathogens.
• OsWRKY6-ox lines displayed leaf lesions, and increased OsWRKY6 levels caused cell death.
• Several pathogenesis-related (PR) genes were constitutively activated, including OsPR10a, and transgenic rice (Oryza sativa) plants overexpressing (ox) OsWRKY6 exhibited enhanced disease resistance to pathogens.
• By contrast, PR gene induction was compromised in transgenic OsWRKY6-RNAi lines, suggesting that OsWRKY6 is a positive regulator of defense responses.
• Our results suggest that OsWRKY6 positively regulates defense responses through activation of OsICS1 expression and OsWRKY6 stabilization.
• This indicates that OsWRKY6 can directly regulate OsICS1 expression and thereby increase SA concentrations.
• OsWRKY transcription factors are involved in various biotic stress responses in rice, and OsWRKY6 specifically acts as an important defense regulator against Xoo.
• However, the relationship between OsWRKY6 and other OsWRKYs, as well as its role in resistance (R) gene-mediated defense, have yet to be studied in depth.
• The OsWRKY6 transcriptional cascade functions in basal defense and Xa1-mediated defense of rice against Xanthomonas oryzae pv. oryzae.
• The rice protein OsWRKY6 directly activates OsWRKY45 and OsWRKY47 expression, and also activates OsPR1a and OsPR1b through the two OsWRKYs, and this transcriptional module participates in Xa1-mediated defense against the pathogen Xanthomonas oryzae pv.
• Here, we characterized a transcriptional cascade triggered by OsWRKY6 that regulated defense against Xoo infection mediated by the NBS-LRR protein Xa1.
• Two OsWRKY6 RNAi knockdown lines showed significantly reduced defense even against an incompatible Xoo infection, and the expression of OsWRKY6 was not regulated by OsWRKY51 and OsWRKY88.
• This study reveals that a novel downstream transcriptional pathway activated by OsWRKY6 is involved in Xa1-mediated defense against Xoo.

Function-related keywords:

• disease ,  defense-response ,  disease-resistance ,  defense ,  leaf ,  resistance ,  cell-death ,  sa ,  SA ,  transcription-factor ,  stress ,  R-protein ,  pathogen ,  biotic-stress ,  xoo ,  stress-response ,  Xoo

Literature:

• Heterologous expression of OsWRKY6 gene in Arabidopsis activates the expression of defense related genes and enhances resistance to pathogens .  DOI:  10.1016/j.plantsci.2011.06.007 ;  PMID:  21763543
• The WRKY Gene Family in Rice (Oryza sativa) .  DOI:  10.1111/j.1744-7909.2007.00504.x
• Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells .  DOI:  10.1104/pp.104.054312 ;  PMID:  15618416
• Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens .  DOI:  10.1111/nph.13516 ;  PMID:  26083148
• The OsWRKY6 transcriptional cascade functions in basal defense and Xa1-mediated defense of rice against Xanthomonas oryzae pv. oryzae .  DOI:  10.1007/s00425-022-03830-5 ;  PMID:  35076864

Related News:

Gene Resources:

• NCBI ID: BK005009
• UniProt accessions:

Sequences: