Gene Details:

Functional Descriptions:

  • Abiotic stress responses of OsiSAP7 were assessed by its overexpression in Arabidopsis under the control of a stress inducible promoter rd29A.
  • Rice OsiSAP7 negatively regulates ABA stress signalling and imparts sensitivity to water-deficit stress in Arabidopsis.
  • Our study suggests that OsiSAP7 acts as a negative regulator of ABA and water-deficit stress signalling by acting as an E3 ubiquitin ligase.
  • OsiSAP7 was chosen to investigate the mechanism of its action based on the dual nature of its sub-cellular localization preferentially in the nucleus or sub-nuclear speckles upon transient expression in onion epidermal cells.
  • OsiSAP7 was localized evenly in the nucleus under unstressed conditions and in sub-nuclear speckles on MG132 treatment.
  • OsiSAP7 exhibits E3 ubiquitin ligase activity in vitro.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g57900.1
    AACTCGCCAACCCACCCTGTACCCGCCGCTCGATCGCCGCCGTTCTCGCCCGAAGAAGGAAAGCGCCATGGCGTCGATGAAGCGGAAGTGCCCCGATGACGAGACGGCCTGCGGCTCCGGCGCCGGCGCCGCCATGTGCGTCACCGGCTGCGGATTCTTCGGCTCCGAGGCCACCAACAACATGTGCTCCCGGTGCTACCGCGAGCACTCCGCGGACAACGACGCCGTGGAGGAGGCGGCGGCAGCCAACAGCGACCTCGAGCTGGTTGGCGTGGCGGAGACGACGACGAAGAAGGCCAGGATGAGCGCCGTCGTCCCCGTCGCCGTCGCGTCATCTTCATCGGCGGCGGCAGAGCAGCCGGCGGCGAAGGCTGCTACGGCGCCGAACCGGTGCGCGGCGTGCCGGAAGAAGGTGGGGCTGACGGGGTTCAAGTGCCGGTGCGGCGGCAACTTCTGTGGCGGCCACCGCCACGCCGACGCCCACGGCTGCGGCTTCGACTACAAGAGCGCCGGGAAGGAGCAGATCGCCAAGCAGAACCCGCTCGTCGTCGCCGACAAGCTGGCCACCCGGATTTGAGCTCTCCTCTCTCTCTTCCATGGAATCCAACAGCAGTCTGCAAAACCATCGACGAGCACAAGCAAACATTGAGTAGCAAGCTCAGTGATCTGTTCTGAAACATGTAAGATTGAATACTTGCATGTAATTTAGTATTTGTTTTCACTGAAGAAACATGTTCCCATTGACAATAATCTTTATTTATGAGCCAAAAGAACAGATCTTTGTTTAGGTGATAGAAATCATCCATTTGTTAAGCTTGTTGTTGATTGATGCACGCATCAAATAGTACAAATTGACACAAGCAGGCTCTGGTTCAATCCCTGCTTACCGGCTTGCACATAATTTTTTAGTGTCAAATAGTCACTTGCTGATTTGCTAT
CDS Sequence
  • >LOC_Os03g57900.1
    ATGGCGTCGATGAAGCGGAAGTGCCCCGATGACGAGACGGCCTGCGGCTCCGGCGCCGGCGCCGCCATGTGCGTCACCGGCTGCGGATTCTTCGGCTCCGAGGCCACCAACAACATGTGCTCCCGGTGCTACCGCGAGCACTCCGCGGACAACGACGCCGTGGAGGAGGCGGCGGCAGCCAACAGCGACCTCGAGCTGGTTGGCGTGGCGGAGACGACGACGAAGAAGGCCAGGATGAGCGCCGTCGTCCCCGTCGCCGTCGCGTCATCTTCATCGGCGGCGGCAGAGCAGCCGGCGGCGAAGGCTGCTACGGCGCCGAACCGGTGCGCGGCGTGCCGGAAGAAGGTGGGGCTGACGGGGTTCAAGTGCCGGTGCGGCGGCAACTTCTGTGGCGGCCACCGCCACGCCGACGCCCACGGCTGCGGCTTCGACTACAAGAGCGCCGGGAAGGAGCAGATCGCCAAGCAGAACCCGCTCGTCGTCGCCGACAAGCTGGCCACCCGGATTTGA
Protein Sequence
  • >LOC_Os03g57900.1
    MASMKRKCPDDETACGSGAGAAMCVTGCGFFGSEATNNMCSRCYREHSADNDAVEEAAAANSDLELVGVAETTTKKARMSAVVPVAVASSSSAAAEQPAAKAATAPNRCAACRKKVGLTGFKCRCGGNFCGGHRHADAHGCGFDYKSAGKEQIAKQNPLVVADKLATRI*