Gene Details:

• MSU gene ID: LOC_Os03g57240
• RAPdb gene ID: Os03g0786400
• Gene Symbol: DST WL1 HST1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Here, we clone and characterize DST (drought and salt tolerance)-a previously unknown zinc finger transcription factor that negatively regulates stomatal closure by direct modulation of genes related to H(2)O(2) homeostasis-and identify a novel pathway for the signal transduction of DST-mediated H(2)O(2)-induced stomatal closure.
• Loss of DST function increases stomatal closure and reduces stomatal density, consequently resulting in enhanced drought and salt tolerance in rice.
• A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control.
• We identify that DST(reg1), a semidominant allele of the DST gene, perturbs DST-directed regulation of OsCKX2 expression and elevates CK levels in the reproductive SAM, leading to increased meristem activity, enhanced panicle branching, and a consequent increase of grain number.
• Here, we report that the zinc finger transcription factor DROUGHT AND SALT TOLERANCE (DST) directly regulates OsCKX2 expression in the reproductive meristem.
• Our study reveals that, as a unique regulator of reproductive meristem activity, DST may be explored to facilitate the genetic enhancement of grain production in rice and other small grain cereals.
• Importantly, the DST(reg1) allele provides an approach to pyramid the Gn1a-dependent and Gn1a-independent effects on grain production.
• Rice zinc finger protein DST enhances grain production through controlling Gn1a/OsCKX2 expression.
• DST-directed expression of OsCKX2 regulates CK accumulation in the SAM and, therefore, controls the number of the reproductive organs.
• The results suggest that OsSRO1c has dual roles in drought and oxidative stress tolerance of rice by promoting stomatal closure and H(2)O(2) accumulation through a novel pathway involving regulators SNAC1 and DST.
• Expression of DST, a reported zinc finger gene negatively regulating H(2)O(2)-induced stomatal closure, and the activity of H(2)O(2)-scavenging related enzymes were significantly suppressed, and H(2)O(2) in guard cells was accumulated in the overexpression lines.
• Plasma membrane receptor-like kinase leaf panicle 2 acts downstream of the DROUGHT AND SALT TOLERANCE transcription factor to regulate drought sensitivity in rice.
• Mediator complex subunit MED25 physically interacts with DST to regulate spikelet number in rice.
• Phenotypic analyses revealed that OsMED25-RNAi and the osmed25 mutant plants exhibited enlarged panicles, with enhanced branching and spikelet number, similar to the DST mutant.
• Thus, OsMED25 was involved in the communication between DST and Pol II general transcriptional machinery to regulate spikelet number.
• Interestingly, we found that WL1 negatively regulated the expression of a narrow leaf gene, NARROW LEAF 1 (NAL1), by recruiting the co-repressor TOPLESS-RELATED PROTEIN and directly binding to the NAL1 regulatory region to inhibit its expression by reducing the chromatin histone acetylation.
• WL1 encodes a Cys-2/His-2-type (C2H2) zinc finger protein that interacts with Tillering and Dwarf 1 (TAD1), a co-activator of the anaphase-promoting complex/cyclosome (APC/C) (a multi-subunit E3 ligase).

Function-related keywords:

• salt-tolerance ,  grain-number ,  meristem ,  salt ,  stomatal ,  grain ,  reproductive ,  drought ,  oxidative ,  branching ,  stomata ,  transcription-factor ,  panicle ,  homeostasis ,  drought-sensitivity ,  spikelet ,  spikelet-number ,  leaf ,  tillering ,  R-protein ,  zinc ,  dwarf

Literature:

• The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice .  DOI:  10.1093/jxb/ers349 ;  PMID:  23202132
• A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control .  DOI:  10.1101/gad.1812409 ;  PMID:  19651988
• Rice zinc finger protein DST enhances grain production through controlling Gn1a/OsCKX2 expression .  DOI:  10.1073/pnas.1300359110 ;  PMID:  23382237
• Plasma membrane receptor-like kinase leaf panicle 2 acts downstream of the DROUGHT AND SALT TOLERANCE transcription factor to regulate drought sensitivity in rice .  DOI:  10.1093/jxb/eru417 ;  PMID:  25385766
• Overexpression of a Chimeric Gene, OsDST-SRDX, Improved Salt Tolerance of Perennial Ryegrass .  DOI:  10.1038/srep27320 ;  PMID:  27251327
• Mediator complex subunit MED25 physically interacts with DST to regulate spikelet number in rice .  DOI:  10.1111/jipb.13238 ;  PMID:  35212455
• The APC/CTAD1-WIDE LEAF 1-NARROW LEAF 1 pathway controls leaf width in rice .  DOI:  10.1093/plcell/koac232 ;  PMID:  35904763

Related News:

Gene Resources:

• NCBI ID: GQ178286
• UniProt accessions:

Sequences: