Gene Details:

• MSU gene ID: LOC_Os03g56950
• RAPdb gene ID: Os03g0782500
• Gene Symbol: OsPIL13 OsPIL1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Oligoarray analysis revealed OsPIL1 downstream genes, which were enriched for cell wall-related genes responsible for cell elongation.
• These data suggest that OsPIL1 functions as a key regulatory factor of reduced plant height via cell wall-related genes in response to drought stress.
• We found that OsPIL1 was highly expressed in the node portions of the stem using promoter-glucuronidase analysis.
• In addition, OsPIL13 and OsPIL15 colocalize with OsPRR1, an ortholog of the Arabidopsis APRR1 gene that controls photoperiodic flowering response through clock function.
• Together, these results suggest that overexpression of OsLF might repress expression of OsGI and Hd1 by competing with OsPRR1 in interacting with OsPIL13 and OsPIL15 and thus induce late flowering.
• An atypical HLH protein OsLF in rice regulates flowering time and interacts with OsPIL13 and OsPIL15.
• Here, we demonstrate that a phytochrome-interacting factor-like protein, OsPIL1/OsPIL13, acts as a key regulator of reduced internode elongation in rice under drought conditions.
• Under drought stress conditions, OsPIL1 expression was inhibited during the light period.
• Rice phytochrome-interacting factor-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress.
• It was found that the expression of OsPIL13 is under the control of circadian rhythms (clock), while the expression of OsPIL15 is negatively regulated by light upon the onset to light exposure of etiolated seedlings.
• The level of OsPIL1 mRNA in rice seedlings grown under nonstressed conditions with light/dark cycles oscillated in a circadian manner with peaks in the middle of the light period.
• Transcriptome analyses showed increased expression of abiotic stress-inducible DREB1A downstream genes and cell elongation-related OsPIL1 downstream genes in the double overexpressors, which suggests that these two transcription factors function independently in the transgenic plants despite the trade-offs required to balance plant growth and stress tolerance.
• The transgenic plants overexpressing both OsPIL1 and DREB1A showed improved drought stress tolerance similar to that of DREB1A overexpressors.
• OsPIL1 is a rice homologue of Arabidopsis PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), and it enhances cell elongation by activating cell wall-related gene expression.
• Here, we show that OsPIL1 negatively regulates leaf senescence in rice.
• Microarray analysis revealed that during DIS, a number of senescence-associated genes were upregulated and OsGLKs, negative regulators of leaf senescence, were strongly repressed in OsPIL1 mutants.
• In addition, OsPIL1 expressed in Arabidopsis upregulates the transcription of ORESARA1, a major senescence-inducing NAC transcription factor and one of the downstream genes of Arabidopsis PIF4, by directly binding the promoter region.
• During dark-induced senescence (DIS), OsPIL1 mutants senesced earlier than wild type; this is opposite to mutants of Arabidopsis PIF4 and PIF5, the closest homologs of OsPIL1.
• In addition, OsPIL1 directly up-regulates the expression of two transcription factor genes, GOLDEN2-LIKE1 (OsGLK1) and OsGLK2.
• Transcriptome analysis revealed that several genes responsible for Chl biosynthesis and photosynthesis were significantly down-regulated in OsPIL1 leaves.
• Thus, OsPIL1 is involved in the promotion of Chl biosynthesis by up-regulating the transcription of OsPORB and OsCAO1 via trifurcate feed-forward regulatory loops involving two OsGLKs.
• The OsPIL1 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 1) gene encodes a basic helix-loop-helix transcription factor.
• Overexpression of OsPIL1 enhanced biomass yield and saccharification efficiency in switchgrass.
• Here, we introduced the OsPIL1 gene into switchgrass by Agrobacterium-mediated transformation with the aim of improving biomass yield of transgenic switchgrass plants.
• The transgenic plants overexpression of OsPIL1 showed increased plant height and biomass yield.
• The results suggest OsPIL1 can be used as a useful molecular tool in improving plant biomass and saccharification efficiency with the purpose of plant fiber biofuel ethanol production.
• OsPIL1 overexpressed transgenic switchgrass plants also released more soluble sugar after enzymatic hydrolysis, indicating improved saccharification efficiency.

Function-related keywords:

• cell-wall ,  cell-elongation ,  stem ,  flower ,  drought ,  height ,  flowering-time ,  seedling ,  growth ,  tolerance ,  transcription-factor ,  abiotic-stress ,  stress ,  biotic-stress ,  drought-stress ,  stress-tolerance ,  plant-growth ,  leaf ,  leaf-senescence ,  senescence ,  photosynthesis ,  Chl-biosynthesis ,  yield ,  biomass ,  plant-height ,  sugar

Literature:

• An atypical HLH protein OsLF in rice regulates flowering time and interacts with OsPIL13 and OsPIL15 .  DOI:  10.1016/j.nbt.2011.04.006 ;  PMID:  21549224
• Characterization of a set of phytochrome-interacting factor-like bHLH proteins in Oryza sativa .  DOI:  10.1271/bbb.60643 ;  PMID:  17485859
• Rice phytochrome-interacting factor-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress .  DOI:  10.1073/pnas.1207324109 ;  PMID:  22984180
• Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants .  DOI:  10.1111/pbi.12644 ;  PMID:  27683092
• Roles of rice PHYTOCHROME-INTERACTING FACTOR-LIKE1 (OsPIL1) in leaf senescence .  DOI:  10.1080/15592324.2017.1362522 ;  PMID:  28805492
• Rice Phytochrome-Interacting Factor-Like1 (OsPIL1) is involved in the promotion of chlorophyll biosynthesis through feed-forward regulatory loops .  DOI:  10.1093/jxb/erx231 ;  PMID:  28922754
• Overexpression of OsPIL1 enhanced biomass yield and saccharification efficiency in switchgrass .  DOI:  10.1016/j.plantsci.2018.08.012 ;  PMID:  30348312

Related News:

Gene Resources:

• NCBI ID: AK105637
• UniProt accessions:

Sequences: