Information report for Pho1
Gene Details
|
|
Functional Descriptions
- The loss of Pho1 caused smaller starch granules to accumulate and modified the amylopectin structure.
- These results strongly suggest that Pho1 plays a crucial role in starch biosynthesis in rice endosperm at low temperatures and that one or more other factors can complement the function of Pho1 at high temperatures.
- The phenotype of the Pho1 mutation was temperature dependent.
- Plastidial phosphorylase (Pho1) accounts for approximately 96% of the total phosphorylase activity in developing rice (Oryza sativa) seeds.
- Variation in the morphological and biochemical phenotype of individual seeds was common to all 15 Pho1-independent homozygous mutant lines studied, indicating that this phenotype was caused solely by the genetic defect.
- Our work suggests that regulation of Pho1 expression via its uORF might be a genetic resource useful-both in natural populations and in the context of genome editing-toward improving plant growth under Pi-deficient conditions.
- We further show that natural accessions lacking the Pho1 uORF exhibit higher Pho1 protein levels and shoot Pi content.
- Increased shoot Pi content was linked to the absence of the Pho1 uORF in a population of F2 segregants.
- In this work, we demonstrate that an upstream open reading frame (uORF) present in the 5’ untranslated region of the Arabidopsis (Arabidopsis thaliana) Pho1 inhibits its translation and influences Pi homeostasis.
- A point mutation removing the Pho1 uORF (
uORF) in transgenic Arabidopsis resulted in increased association of its mRNA with polysomes and led to higher Pho1 protein levels, independent of Pi availability. - Pho1 deficiency impaired MOS mobilization, triggering short MOS accumulation and starch synthesis reduction during early seed development.
- Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm.
- These findings show that Pho1 cooperates with DPE1 to control short MOS mobilization during starch synthesis initiation in rice endosperm.
Functional Keywords
- starch , starch-biosynthesis , temperature , seed , endosperm , growth , shoot , homeostasis , plant-growth , Pi , pi , Pi-homeostasis , development , seed-development
Literature and News
- Mutation of the plastidial alpha-glucan phosphorylase gene in rice affects the synthesis and structure of starch in the endosperm . DOI: 10.1105/tpc.107.054007 ; PMID: 18621947
- Rice endosperm starch phosphorylase Pho1 assembles with disproportionating enzyme Dpe1 to form a protein complex that enhances synthesis of malto-oligosaccharides . DOI: 10.1074/jbc.M116.735449 ; PMID: 27502283
- Modulation of Shoot Phosphate Level and Growth by PHOSPHATE1 Upstream Open Reading Frame . DOI: 10.1104/pp.19.01549 ; PMID: 32327548
- Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm . DOI: 10.1007/s00122-023-04250-z ; PMID: 36912930
Gene Resources
- UniProt: Q0DNE4
- EMBL: AP008209
- AlphaFoldDB: Q0DNE4
- KEGG: dosa:Os03g0758100
- InterPro: IPR000811, IPR011833, IPR035090
- PANTHER: PTHR11468, PTHR11468:SF28
- SUPFAM: SSF53756
- PROSITE: PS00102
- Gene3D: 3.40.50.2000
- SWISS-MODEL: Q0DNE4
Sequences
cDNA Sequence
- >LOC_Os03g55090.1
ATGGCGACCGCCTCGGCGCCGCTGCAGCTGGCCACCGCGTCCCGGCCGCTCCCCGTCGGCGTCGGCTGCGGCGGAGGAGGAGGCGGGGGGCTCCACGTGGGTGGTGCCCGCGGCGGGGGCGCGGCACCGGCGCGGCGGCGGCTGGCGGTGCGGAGCGTGGCGAGCGATCGGGGCGTGCAGGGGTCGGTGTCGCCCGAGGAAGAGATTTCAAGTGTGCTAAATTCCATCGATTCCTCTACCATTGCATCAAACATTAAGCACCATGCGGAGTTCACACCAGTATTCTCTCCAGAGCACTTTTCACCTCTGAAGGCTTACCATGCAACTGCTAAAAGTGTTCTTGATACTCTGATAATGAACTGGAATGCAACATATGACTATTACGACAGAACAAATGTGAAGCAAGCGTATTACCTGTCCATGGAGTTTTTACAGGGAAGAGCTCTCACTAATGCCGTTGGTAACCTTGAGCTAACTGGACAATACGCAGAAGCACTACAACAACTTGGACACAGCCTAGAGGATGTTGCTACCCAGGAGCCAGATGCTGCCCTTGGGAATGGTGGTCTAGGCCGGTTAGCTTCCTGTTTCTTGGATTCTCTGGCAACCCTAAATTATCCAGCATGGGGATATGGACTTCGATACAAACATGGCCTCTTTAAAGCAAATCATACGAAGGATGGTCAGGAGGAGGTAGCTGAAAATTGGCTCGAGATGGGAAATCCTTGGGAGATTGTAAGAACCGATGTCTCCTATCCTGTGAAGTTCTATGGTAAAGTGGTTGAAGGCACTGATGGGAGGATGCACTGGATTGGAGGAGAAAATATCAAGGTTGTTGCTCATGATATCCCTATTCCTGGCTACAAGACTAAAACTACCAACAATCTTCGTCTTTGGTCAACAACAGTGCCATCACAAGATTTCGATTTGGAAGCTTTTAATGCTGGAGATCATGCAAGTGCATATGAAGCTCATCTAAATGCTGAAAAGCCTCACTACAGGATATTATTGCTCGTTTCGAGAGGAGAGCTGGTGATTCTCTCAGCTGGGAGGACTTCCCCTCTAAAGTTGCAGTGCAGATGAATGACACTCACCCAACACTGTGCATTCCTGAGTTGATGAGAATATTGATTGATGTTAAAGGGTTAAGCTGGAATGAGGCTTGGAGTATCACAGAAAGAACTGTGGCATACACAAACCACACGGTGCTTCCTGAAGCTCTGGAGAAGTGGAGCTTGGACATAATGCAGAAACTTCTTCCTCGGCATGTTGAAATCATAGAAAAAATTGATGGGGAGCTGATGAACATCATTATCTCAAAATACGGAACAGAAGATACTTCACTGTTAAAAAAGAAGATTAAAGAAATGAGAATCTTAGACAACATTGACCTACCAGATTCTATTGCCAAACTATTTGTGAAACCAAAAGAGAAAAAAGAATCTCCTGCTAAATTGAAAGAGAAATTGCTTGTCAAATCTCTGGAGCCTAGTGTTGTGGTTGAGGAGAAAACTGTGTCCAAAGTAGAGATAAACGAGGACTCTGAGGAGGTGGAGGTAGACTCTGAAGAAGTTGTGGAGGCAGAAAACGAGGACTCTGAGGATGAGTTAGATCCATTTGTAAAATCAGATCCTAAATTACCTAGAGTTGTCCGAATGGCTAACCTTTGTGTTGTTGGTGGGCATTCGGTTAATGGTGTGGCTGCGATTCACAGCGAGATTGTGAAAGAAGATGTATTCAACAGCTTTTATGAGATGTGGCCCGCTAAATTTCAAAATAAAACAAATGGAGTGACTCCTAGACGTTGGATTCGGTTTTGTAATCCTGAATTAAGTGCAATCATTTCAAAATGGATAGGATCTGATGATTGGGTTTTGAACACTGATAAACTTGCTGAATTAAAGAAGTTTGCTGATGATGAGGATCTGCAATCAGAATGGCGTGCTGCTAAAAAGGCTAACAAGGTGAAGGTTGTTTCTCTCATAAGAGAAAAAACAGGATATATCGTCAGTCCAGATGCAATGTTTGACGTTCAGGTGAAAAGGATCCATGAGTATAAGCGACAGCTGCTAAATATCCTTGGAATTGTCTACCGCTACAAGAAGATGAAAGAAATGAGTGCAAAAGACAGAATAAATAGCTTTGTTCCAAGGGTATGCATATTTGGTGGGAAAGCATTTGCCACTTACGTACAGGCAAAGAGGATAGTGAAGTTTATTACAGATGTTGCAGCTACTGTAAATCATGATCCAGAAATTGGAGATCTATTGAAGGTTGTATTTATTCCAGATTATAATGTTAGTGTTGCTGAGGCGCTAATCCCTGCCAGTGAATTGTCTCAGCATATCAGTACTGCTGGAATGGAAGCTAGTGGAACCAGCAACATGAAGTTTGCAATGAATGGATGTATCCTTATTGGAACTTTGGATGGTGCTAATGTGGAAATCAGAGAGGAGGTTGGAGAGGAAAACTTTTTCCTTTTTGGTGCTGAGGCACATGAAATTGCTGGTTTAAGGAAAGAGAGAGCCCAGGGAAAGTTTGTGCCTGACCCAAGATTCGAAGAGGTTAAGAGATTTGTCCGCAGTGGGGTCTTTGGAACTTACAACTACGATGACTTGATGGGTTCTCTGGAAGGAAATGAAGGTTATGGGCGTGCAGACTATTTTCTTGTTGGTAAAGATTTCCCCAGCTACATTGAATGCCAGGAGAAGGTTGATAAAGCATACCGCGATCAGAAACTATGGACAAGGATGTCAATCCTCAACACAGCCAGTTCCTCCAAGTTCAACAGCGACCGGACGATTCACGAGTACGCCAAGGACATCTGGGACATCAAGCCTGTCATCCTGCCCTAG
CDS Sequence
- >LOC_Os03g55090.1
ATGAATGACACTCACCCAACACTGTGCATTCCTGAGTTGATGAGAATATTGATTGATGTTAAAGGGTTAAGCTGGAATGAGGCTTGGAGTATCACAGAAAGAACTGTGGCATACACAAACCACACGGTGCTTCCTGAAGCTCTGGAGAAGTGGAGCTTGGACATAATGCAGAAACTTCTTCCTCGGCATGTTGAAATCATAGAAAAAATTGATGGGGAGCTGATGAACATCATTATCTCAAAATACGGAACAGAAGATACTTCACTGTTAAAAAAGAAGATTAAAGAAATGAGAATCTTAGACAACATTGACCTACCAGATTCTATTGCCAAACTATTTGTGAAACCAAAAGAGAAAAAAGAATCTCCTGCTAAATTGAAAGAGAAATTGCTTGTCAAATCTCTGGAGCCTAGTGTTGTGGTTGAGGAGAAAACTGTGTCCAAAGTAGAGATAAACGAGGACTCTGAGGAGGTGGAGGTAGACTCTGAAGAAGTTGTGGAGGCAGAAAACGAGGACTCTGAGGATGAGTTAGATCCATTTGTAAAATCAGATCCTAAATTACCTAGAGTTGTCCGAATGGCTAACCTTTGTGTTGTTGGTGGGCATTCGGTTAATGGTGTGGCTGCGATTCACAGCGAGATTGTGAAAGAAGATGTATTCAACAGCTTTTATGAGATGTGGCCCGCTAAATTTCAAAATAAAACAAATGGAGTGACTCCTAGACGTTGGATTCGGTTTTGTAATCCTGAATTAAGTGCAATCATTTCAAAATGGATAGGATCTGATGATTGGGTTTTGAACACTGATAAACTTGCTGAATTAAAGAAGTTTGCTGATGATGAGGATCTGCAATCAGAATGGCGTGCTGCTAAAAAGGCTAACAAGGTGAAGGTTGTTTCTCTCATAAGAGAAAAAACAGGATATATCGTCAGTCCAGATGCAATGTTTGACGTTCAGGTGAAAAGGATCCATGAGTATAAGCGACAGCTGCTAAATATCCTTGGAATTGTCTACCGCTACAAGAAGATGAAAGAAATGAGTGCAAAAGACAGAATAAATAGCTTTGTTCCAAGGGTATGCATATTTGGTGGGAAAGCATTTGCCACTTACGTACAGGCAAAGAGGATAGTGAAGTTTATTACAGATGTTGCAGCTACTGTAAATCATGATCCAGAAATTGGAGATCTATTGAAGGTTGTATTTATTCCAGATTATAATGTTAGTGTTGCTGAGGCGCTAATCCCTGCCAGTGAATTGTCTCAGCATATCAGTACTGCTGGAATGGAAGCTAGTGGAACCAGCAACATGAAGTTTGCAATGAATGGATGTATCCTTATTGGAACTTTGGATGGTGCTAATGTGGAAATCAGAGAGGAGGTTGGAGAGGAAAACTTTTTCCTTTTTGGTGCTGAGGCACATGAAATTGCTGGTTTAAGGAAAGAGAGAGCCCAGGGAAAGTTTGTGCCTGACCCAAGATTCGAAGAGGTTAAGAGATTTGTCCGCAGTGGGGTCTTTGGAACTTACAACTACGATGACTTGATGGGTTCTCTGGAAGGAAATGAAGGTTATGGGCGTGCAGACTATTTTCTTGTTGGTAAAGATTTCCCCAGCTACATTGAATGCCAGGAGAAGGTTGATAAAGCATACCGCGATCAGAAACTATGGACAAGGATGTCAATCCTCAACACAGCCAGTTCCTCCAAGTTCAACAGCGACCGGACGATTCACGAGTACGCCAAGGACATCTGGGACATCAAGCCTGTCATCCTGCCCTAG
Protein Sequence
- >LOC_Os03g55090.1
MNDTHPTLCIPELMRILIDVKGLSWNEAWSITERTVAYTNHTVLPEALEKWSLDIMQKLLPRHVEIIEKIDGELMNIIISKYGTEDTSLLKKKIKEMRILDNIDLPDSIAKLFVKPKEKKESPAKLKEKLLVKSLEPSVVVEEKTVSKVEINEDSEEVEVDSEEVVEAENEDSEDELDPFVKSDPKLPRVVRMANLCVVGGHSVNGVAAIHSEIVKEDVFNSFYEMWPAKFQNKTNGVTPRRWIRFCNPELSAIISKWIGSDDWVLNTDKLAELKKFADDEDLQSEWRAAKKANKVKVVSLIREKTGYIVSPDAMFDVQVKRIHEYKRQLLNILGIVYRYKKMKEMSAKDRINSFVPRVCIFGGKAFATYVQAKRIVKFITDVAATVNHDPEIGDLLKVVFIPDYNVSVAEALIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEENFFLFGAEAHEIAGLRKERAQGKFVPDPRFEEVKRFVRSGVFGTYNYDDLMGSLEGNEGYGRADYFLVGKDFPSYIECQEKVDKAYRDQKLWTRMSILNTASSSKFNSDRTIHEYAKDIWDIKPVILP*