Gene Details:

Functional Descriptions:

  • The loss of Pho1 caused smaller starch granules to accumulate and modified the amylopectin structure.
  • These results strongly suggest that Pho1 plays a crucial role in starch biosynthesis in rice endosperm at low temperatures and that one or more other factors can complement the function of Pho1 at high temperatures.
  • The phenotype of the Pho1 mutation was temperature dependent.
  • Plastidial phosphorylase (Pho1) accounts for approximately 96% of the total phosphorylase activity in developing rice (Oryza sativa) seeds.
  • Variation in the morphological and biochemical phenotype of individual seeds was common to all 15 Pho1-independent homozygous mutant lines studied, indicating that this phenotype was caused solely by the genetic defect.
  • Our work suggests that regulation of Pho1 expression via its uORF might be a genetic resource useful-both in natural populations and in the context of genome editing-toward improving plant growth under Pi-deficient conditions.
  • We further show that natural accessions lacking the Pho1 uORF exhibit higher Pho1 protein levels and shoot Pi content.
  • Increased shoot Pi content was linked to the absence of the Pho1 uORF in a population of F2 segregants.
  • In this work, we demonstrate that an upstream open reading frame (uORF) present in the 5’ untranslated region of the Arabidopsis (Arabidopsis thaliana) Pho1 inhibits its translation and influences Pi homeostasis.
  • A point mutation removing the Pho1 uORF (uORF) in transgenic Arabidopsis resulted in increased association of its mRNA with polysomes and led to higher Pho1 protein levels, independent of Pi availability.
  • Pho1 deficiency impaired MOS mobilization, triggering short MOS accumulation and starch synthesis reduction during early seed development.
  • Pho1 cooperates with DPE1 to control short maltooligosaccharide mobilization during starch synthesis initiation in rice endosperm.
  • These findings show that Pho1 cooperates with DPE1 to control short MOS mobilization during starch synthesis initiation in rice endosperm.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g55090.1
    ATGGCGACCGCCTCGGCGCCGCTGCAGCTGGCCACCGCGTCCCGGCCGCTCCCCGTCGGCGTCGGCTGCGGCGGAGGAGGAGGCGGGGGGCTCCACGTGGGTGGTGCCCGCGGCGGGGGCGCGGCACCGGCGCGGCGGCGGCTGGCGGTGCGGAGCGTGGCGAGCGATCGGGGCGTGCAGGGGTCGGTGTCGCCCGAGGAAGAGATTTCAAGTGTGCTAAATTCCATCGATTCCTCTACCATTGCATCAAACATTAAGCACCATGCGGAGTTCACACCAGTATTCTCTCCAGAGCACTTTTCACCTCTGAAGGCTTACCATGCAACTGCTAAAAGTGTTCTTGATACTCTGATAATGAACTGGAATGCAACATATGACTATTACGACAGAACAAATGTGAAGCAAGCGTATTACCTGTCCATGGAGTTTTTACAGGGAAGAGCTCTCACTAATGCCGTTGGTAACCTTGAGCTAACTGGACAATACGCAGAAGCACTACAACAACTTGGACACAGCCTAGAGGATGTTGCTACCCAGGAGCCAGATGCTGCCCTTGGGAATGGTGGTCTAGGCCGGTTAGCTTCCTGTTTCTTGGATTCTCTGGCAACCCTAAATTATCCAGCATGGGGATATGGACTTCGATACAAACATGGCCTCTTTAAAGCAAATCATACGAAGGATGGTCAGGAGGAGGTAGCTGAAAATTGGCTCGAGATGGGAAATCCTTGGGAGATTGTAAGAACCGATGTCTCCTATCCTGTGAAGTTCTATGGTAAAGTGGTTGAAGGCACTGATGGGAGGATGCACTGGATTGGAGGAGAAAATATCAAGGTTGTTGCTCATGATATCCCTATTCCTGGCTACAAGACTAAAACTACCAACAATCTTCGTCTTTGGTCAACAACAGTGCCATCACAAGATTTCGATTTGGAAGCTTTTAATGCTGGAGATCATGCAAGTGCATATGAAGCTCATCTAAATGCTGAAAAGCCTCACTACAGGATATTATTGCTCGTTTCGAGAGGAGAGCTGGTGATTCTCTCAGCTGGGAGGACTTCCCCTCTAAAGTTGCAGTGCAGATGAATGACACTCACCCAACACTGTGCATTCCTGAGTTGATGAGAATATTGATTGATGTTAAAGGGTTAAGCTGGAATGAGGCTTGGAGTATCACAGAAAGAACTGTGGCATACACAAACCACACGGTGCTTCCTGAAGCTCTGGAGAAGTGGAGCTTGGACATAATGCAGAAACTTCTTCCTCGGCATGTTGAAATCATAGAAAAAATTGATGGGGAGCTGATGAACATCATTATCTCAAAATACGGAACAGAAGATACTTCACTGTTAAAAAAGAAGATTAAAGAAATGAGAATCTTAGACAACATTGACCTACCAGATTCTATTGCCAAACTATTTGTGAAACCAAAAGAGAAAAAAGAATCTCCTGCTAAATTGAAAGAGAAATTGCTTGTCAAATCTCTGGAGCCTAGTGTTGTGGTTGAGGAGAAAACTGTGTCCAAAGTAGAGATAAACGAGGACTCTGAGGAGGTGGAGGTAGACTCTGAAGAAGTTGTGGAGGCAGAAAACGAGGACTCTGAGGATGAGTTAGATCCATTTGTAAAATCAGATCCTAAATTACCTAGAGTTGTCCGAATGGCTAACCTTTGTGTTGTTGGTGGGCATTCGGTTAATGGTGTGGCTGCGATTCACAGCGAGATTGTGAAAGAAGATGTATTCAACAGCTTTTATGAGATGTGGCCCGCTAAATTTCAAAATAAAACAAATGGAGTGACTCCTAGACGTTGGATTCGGTTTTGTAATCCTGAATTAAGTGCAATCATTTCAAAATGGATAGGATCTGATGATTGGGTTTTGAACACTGATAAACTTGCTGAATTAAAGAAGTTTGCTGATGATGAGGATCTGCAATCAGAATGGCGTGCTGCTAAAAAGGCTAACAAGGTGAAGGTTGTTTCTCTCATAAGAGAAAAAACAGGATATATCGTCAGTCCAGATGCAATGTTTGACGTTCAGGTGAAAAGGATCCATGAGTATAAGCGACAGCTGCTAAATATCCTTGGAATTGTCTACCGCTACAAGAAGATGAAAGAAATGAGTGCAAAAGACAGAATAAATAGCTTTGTTCCAAGGGTATGCATATTTGGTGGGAAAGCATTTGCCACTTACGTACAGGCAAAGAGGATAGTGAAGTTTATTACAGATGTTGCAGCTACTGTAAATCATGATCCAGAAATTGGAGATCTATTGAAGGTTGTATTTATTCCAGATTATAATGTTAGTGTTGCTGAGGCGCTAATCCCTGCCAGTGAATTGTCTCAGCATATCAGTACTGCTGGAATGGAAGCTAGTGGAACCAGCAACATGAAGTTTGCAATGAATGGATGTATCCTTATTGGAACTTTGGATGGTGCTAATGTGGAAATCAGAGAGGAGGTTGGAGAGGAAAACTTTTTCCTTTTTGGTGCTGAGGCACATGAAATTGCTGGTTTAAGGAAAGAGAGAGCCCAGGGAAAGTTTGTGCCTGACCCAAGATTCGAAGAGGTTAAGAGATTTGTCCGCAGTGGGGTCTTTGGAACTTACAACTACGATGACTTGATGGGTTCTCTGGAAGGAAATGAAGGTTATGGGCGTGCAGACTATTTTCTTGTTGGTAAAGATTTCCCCAGCTACATTGAATGCCAGGAGAAGGTTGATAAAGCATACCGCGATCAGAAACTATGGACAAGGATGTCAATCCTCAACACAGCCAGTTCCTCCAAGTTCAACAGCGACCGGACGATTCACGAGTACGCCAAGGACATCTGGGACATCAAGCCTGTCATCCTGCCCTAG
CDS Sequence
  • >LOC_Os03g55090.1
    ATGAATGACACTCACCCAACACTGTGCATTCCTGAGTTGATGAGAATATTGATTGATGTTAAAGGGTTAAGCTGGAATGAGGCTTGGAGTATCACAGAAAGAACTGTGGCATACACAAACCACACGGTGCTTCCTGAAGCTCTGGAGAAGTGGAGCTTGGACATAATGCAGAAACTTCTTCCTCGGCATGTTGAAATCATAGAAAAAATTGATGGGGAGCTGATGAACATCATTATCTCAAAATACGGAACAGAAGATACTTCACTGTTAAAAAAGAAGATTAAAGAAATGAGAATCTTAGACAACATTGACCTACCAGATTCTATTGCCAAACTATTTGTGAAACCAAAAGAGAAAAAAGAATCTCCTGCTAAATTGAAAGAGAAATTGCTTGTCAAATCTCTGGAGCCTAGTGTTGTGGTTGAGGAGAAAACTGTGTCCAAAGTAGAGATAAACGAGGACTCTGAGGAGGTGGAGGTAGACTCTGAAGAAGTTGTGGAGGCAGAAAACGAGGACTCTGAGGATGAGTTAGATCCATTTGTAAAATCAGATCCTAAATTACCTAGAGTTGTCCGAATGGCTAACCTTTGTGTTGTTGGTGGGCATTCGGTTAATGGTGTGGCTGCGATTCACAGCGAGATTGTGAAAGAAGATGTATTCAACAGCTTTTATGAGATGTGGCCCGCTAAATTTCAAAATAAAACAAATGGAGTGACTCCTAGACGTTGGATTCGGTTTTGTAATCCTGAATTAAGTGCAATCATTTCAAAATGGATAGGATCTGATGATTGGGTTTTGAACACTGATAAACTTGCTGAATTAAAGAAGTTTGCTGATGATGAGGATCTGCAATCAGAATGGCGTGCTGCTAAAAAGGCTAACAAGGTGAAGGTTGTTTCTCTCATAAGAGAAAAAACAGGATATATCGTCAGTCCAGATGCAATGTTTGACGTTCAGGTGAAAAGGATCCATGAGTATAAGCGACAGCTGCTAAATATCCTTGGAATTGTCTACCGCTACAAGAAGATGAAAGAAATGAGTGCAAAAGACAGAATAAATAGCTTTGTTCCAAGGGTATGCATATTTGGTGGGAAAGCATTTGCCACTTACGTACAGGCAAAGAGGATAGTGAAGTTTATTACAGATGTTGCAGCTACTGTAAATCATGATCCAGAAATTGGAGATCTATTGAAGGTTGTATTTATTCCAGATTATAATGTTAGTGTTGCTGAGGCGCTAATCCCTGCCAGTGAATTGTCTCAGCATATCAGTACTGCTGGAATGGAAGCTAGTGGAACCAGCAACATGAAGTTTGCAATGAATGGATGTATCCTTATTGGAACTTTGGATGGTGCTAATGTGGAAATCAGAGAGGAGGTTGGAGAGGAAAACTTTTTCCTTTTTGGTGCTGAGGCACATGAAATTGCTGGTTTAAGGAAAGAGAGAGCCCAGGGAAAGTTTGTGCCTGACCCAAGATTCGAAGAGGTTAAGAGATTTGTCCGCAGTGGGGTCTTTGGAACTTACAACTACGATGACTTGATGGGTTCTCTGGAAGGAAATGAAGGTTATGGGCGTGCAGACTATTTTCTTGTTGGTAAAGATTTCCCCAGCTACATTGAATGCCAGGAGAAGGTTGATAAAGCATACCGCGATCAGAAACTATGGACAAGGATGTCAATCCTCAACACAGCCAGTTCCTCCAAGTTCAACAGCGACCGGACGATTCACGAGTACGCCAAGGACATCTGGGACATCAAGCCTGTCATCCTGCCCTAG
Protein Sequence
  • >LOC_Os03g55090.1
    MNDTHPTLCIPELMRILIDVKGLSWNEAWSITERTVAYTNHTVLPEALEKWSLDIMQKLLPRHVEIIEKIDGELMNIIISKYGTEDTSLLKKKIKEMRILDNIDLPDSIAKLFVKPKEKKESPAKLKEKLLVKSLEPSVVVEEKTVSKVEINEDSEEVEVDSEEVVEAENEDSEDELDPFVKSDPKLPRVVRMANLCVVGGHSVNGVAAIHSEIVKEDVFNSFYEMWPAKFQNKTNGVTPRRWIRFCNPELSAIISKWIGSDDWVLNTDKLAELKKFADDEDLQSEWRAAKKANKVKVVSLIREKTGYIVSPDAMFDVQVKRIHEYKRQLLNILGIVYRYKKMKEMSAKDRINSFVPRVCIFGGKAFATYVQAKRIVKFITDVAATVNHDPEIGDLLKVVFIPDYNVSVAEALIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEENFFLFGAEAHEIAGLRKERAQGKFVPDPRFEEVKRFVRSGVFGTYNYDDLMGSLEGNEGYGRADYFLVGKDFPSYIECQEKVDKAYRDQKLWTRMSILNTASSSKFNSDRTIHEYAKDIWDIKPVILP*