Gene Details:

Functional Descriptions:

  • A rice ortholog of Arabidopsis APETALA1, OsMADS14, was expressed in the floral meristem in wild-type but not in RFT1 RNAi plants, suggesting that OsMADS14 is activated by RFT1 protein in the SAM after the transition to flowering.
  • RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O.
  • Transcript levels of three flowering regulators-Ehd1, OsMADS14, and Hd3a-were decreased in these mutants, whereas those of OsGI and Hd1 were unchanged.
  • These results indicate that OsMADS51 is a flowering promoter, particularly in SDs, and that this gene functions upstream of Ehd1, OsMADS14, and Hd3a.
  • OsMADS51 is a short-day flowering promoter that functions upstream of Ehd1, OsMADS14, and Hd3a.
  • These results suggested that OsDof12 might regulate flowering by controlling the expression of Hd3a and OsMADS14.
  • We demonstrate that OsMADS14 and OsMADS15, in addition to their function of specifying meristem identity, are also required to specify palea and lodicule identities.
  • Here we show that two null mutations of OsMADS14 result in a shrunken and chalky grain phenotype.
  • OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm.
  • Transcriptomic profiling analyses revealed that the loss-of-function of OsMADS14 leads to significantly downregulated expression of many core starch synthesis genes, including OsAGPL2 and Waxy.
  • Our study thus demonstrates that OsMADS14 plays an essential role in the synthesis of storage starch and provides novel insights into the underlying molecular mechanism that may be used to improve rice cultivars by molecular breeding.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g54160.2
    TCGTCGTCGTCGTCTCCCCCACTCGATCGATCCATCCATCGATCGATCGGTCCCCCCCATCGCGCGCGACGCATTCCGCCGCCGTCTCGCCGTGTCCACGTGATGGGGGCCGGGGCTAGGGGATAGGCGGATAGCCAGCAGCCACCACCACCAGTAGTTGCCGTGTGGGGATAGGTGTGGCTATAGGGCTAGTGGTCGTCGCTGATAGCGAGGTGGGTAGGGTTAATTTTGGTTGGAGGTAGAGAGAGAGAGAGAGGGAGGGAGGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAACAGGAGGAAGATGGGGCGGGGCAAGGTGCAGCTGAAGCGGATCGAGAACAAGATCAACCGGCAGGTGACCTTCTCCAAGCGCAGGTCGGGGCTGCTCAAGAAGGCGAATGAGATCTCCGTGCTCTGCGACGCCGAGGTCGCGCTCATCATCTTCTCCACCAAGGGCAAGCTCTACGAGTACGCCACCGACTCATGTATGGACAAAATCCTTGAACGTTATGAGCGCTACTCCTATGCAGAAAAGGTCCTTATTTCAGCTGAATCTGACACTCAGGGCAACTGGTGCCACGAATATAGGAAACTGAAGGCTAAGGTTGAGACAATACAGAAATGTCAAAAGCACCTCATGGGAGAGGATCTTGAATCTTTGAATCTCAAAGAGCTGCAGCAGCTGGAGCAGCAGCTGGAAAATTCGTTGAAACATATCAGATCCAGAAAGAGCCAACTAATGCTCGAGTCCATTAACGAGCTTCAACGGAAGGAAAAGTCACTGCAGGAGGAGAATAAGGTCCTACAGAAAGAACTGGTGGAGAAGCAGAAAGTCCAGAAGCAACAAGTGCAATGGGACCAGACACAACCTCAAACAAGTTCCTCATCATCCTCCTTCATGATGAGGGAAGCCCTTCCAACAACTAATATCAGTAACTACCCTGCAGCAGCTGGCGAAAGGATAGAGGATGTAGCAGCAGGGCAGCCACAGCATGTTCGCATTGGGCTGCCACCATGGATGCTGAGCCACATCAACGGCTAAGGAGGCTTCAGATCCATACCAGTAATCACAAGTTGCAACCTGACCCGGTCCGGTCGCCTGCTGCTCTGGTTTACTACTAGTACTATTGTCATCTTGCGGTTGCGAGACGAGGAAAGCATTTTAGCCCTAAATTCAGCATTAGTAGCAAGCTGCAATGTGTATATTTTGGCTTCGTCCAGCACCGTCTTCCTCCCACCAGTAATTTACCCATGTAATATATGCGAGTAGCATGAACAAATTTTCCCGTTTCCAACCATCTCCATTGGTGTCATGTGTGACTTAAATAGCGAAATTTCAGCATTGTGCATAGTGTGATTACTGTAAGATAAATAAACTTTGTAGACAATAAGTCTCCGTTATCTTGCTGATTGGAGCTGAATCTGTCCGATCACCGGCAGAGCTGATTGAGCGCATACTGCATAACGAATAAATTGTATGCGAACAAGTTGATAGGCATAAATCGTTCGACTGTTTACAAAGAAAATAAGGCGTGACATTCTCAGTTAAATAATGGGAGATCATCTTGTATATAATGATCTTCTGCCGTCACTCCTATACAAACATTTTACCCATTACAAAAACCAAGAAATATACCAAGGCGCCCAAGCTCACTGTCACTGCTGACTCCCCTACCACTAAAAATTCTCAAGAGAGGATAACGAATGCTAAGCATTAGTCTTCTAAGAATGGCATTCAGAACTGTGGAACTATGTGCCGGCTGATGAGAACTCAGCCTATGAATAAATTAATCTAGCCACAACTTTAACATAAAGATATTGGTCAGAGGTTGTATTTAACTGAACAGGGATATGCTAGTATTAAACTCGAAGCATCTTCTCTTTCAGGCGTGAAATGAACTTGAATGTCTCCTCTGCTTTGACCTGTTGATACATATCACTTCTTGATACTCTGTCCGGATGGAATTTCAAAAGAGCTT
  • >LOC_Os03g54160.1
    TCGTCGTCGTCGTCTCCCCCACTCGATCGATCCATCCATCGATCGATCGGTCCCCCCCATCGCGCGCGACGCATTCCGCCGCCGTCTCGCCGTGTCCACGTGATGGGGGCCGGGGCTAGGGGATAGGCGGATAGCCAGCAGCCACCACCACCAGTAGTTGCCGTGTGGGGATAGGTGTGGCTATAGGGCTAGTGGTCGTCGCTGATAGCGAGGTGGGTAGGGTTAATTTTGGTTGGAGGTAGAGAGAGAGAGAGAGGGAGGGAGGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAACAGGAGGAAGATGGGGCGGGGCAAGGTGCAGCTGAAGCGGATCGAGAACAAGATCAACCGGCAGGTGACCTTCTCCAAGCGCAGGTCGGGGCTGCTCAAGAAGGCGAATGAGATCTCCGTGCTCTGCGACGCCGAGGTCGCGCTCATCATCTTCTCCACCAAGGGCAAGCTCTACGAGTACGCCACCGACTCATGTATGGACAAAATCCTTGAACGTTATGAGCGCTACTCCTATGCAGAAAAGGTCCTTATTTCAGCTGAATCTGACACTCAGGGCAACTGGTGCCACGAATATAGGAAACTGAAGGCTAAGGTTGAGACAATACAGAAATGTCAAAAGCACCTCATGGGAGAGGATCTTGAATCTTTGAATCTCAAAGAGCTGCAGCAGCTGGAGCAGCAGCTGGAAAATTCGTTGAAACATATCAGATCCAGAAAGAGCCAACTAATGCTCGAGTCCATTAACGAGCTTCAACGGAAGGAAAAGTCACTGCAGGAGGAGAATAAGGTCCTACAGAAAGAACTGGTGGAGAAGCAGAAAGTCCAGAAGCAACAAGTGCAATGGGACCAGACACAACCTCAAACAAGTTCCTCATCATCCTCCTTCATGATGAGGGAAGCCCTTCCAACAACTAATATCAGTAACTACCCTGCAGCAGCTGGCGAAAGGATAGAGGATGTAGCAGCAGGGCAGCCACAGCATGTTCGCATTGGGCTGCCACCATGGATGCTGAGCCACATCAACGGCTAAGGAGGCTTCAGATCCATACCAGTAATCACAAGTTGCAACCTGACCCGGTCCGGTCGCCTGCTGCTCTGGTTTACTACTAGTACTATTGTCATCTTGCGGTTGCGAGACGAGGAAAGCATTTTAGCCCTAAATTCAGCATTAGTAGCAAGCTGCAATGTGTATATTTTGGCTTCGTCCAGCACCGTCTTCCTCCCACCAGTAATTTACCCATGTAATATATGCGAGTAGCATGAACAAATTTTCCCGTTTCCAACCATCTCCATTGGTGTCATGTGTGACTTAAATAGCGAAATTTCAGCATTGTGCATAGTGTGATTACTGTAAGATAAATAAACTTTGTAGACAATAAGTCTCCGTTATCTTGCTGATTGGAGCTGAATCTGTCCGATCACCGGCAGAGCTGATTGAGCGCATACTGCATAACGAATAAATTGTATGCGAACAAGTTGATAGGCATAAATCGTTCGACTGTTTACAAAGAAAATAAGGCGTGACATTCTCAGTTAAATAATGGGAGATCATCTTGTATATAATGATCTTCTGCCGTCACTCCTATACAAACATTTTACCCATTACAAAAACCAAGAAATATACCAAGGCGCCCAAGCTCACTGTCACTGCTGACTCCCCTACCACTAAAAATTCTCAAGAGAGGATAACGAATGCTAAGCATTAGTCTTCTAAGAATGGCATTCAGAACTGTGGAACTATGTGCCGGCTGATGAGAACTCAGCCTATGAATAAATTAATCTAGCCACAACTTTAACATAAAGATATTGGTCAGAGGTTGTATTTAACTGAACAGGGATATGCTAGTATTAAACTCGAAGCATCTTCTCTTTCAGGCGTGAAATGAACTTGAATGTCTCCTCTGCTTTGACCTGTTGATACATATCACTTCTTGATACTCTGTCCGGATGGAATTTCAAAAGAGCTT
CDS Sequence
  • >LOC_Os03g54160.2
    ATGGGGCGGGGCAAGGTGCAGCTGAAGCGGATCGAGAACAAGATCAACCGGCAGGTGACCTTCTCCAAGCGCAGGTCGGGGCTGCTCAAGAAGGCGAATGAGATCTCCGTGCTCTGCGACGCCGAGGTCGCGCTCATCATCTTCTCCACCAAGGGCAAGCTCTACGAGTACGCCACCGACTCATGTATGGACAAAATCCTTGAACGTTATGAGCGCTACTCCTATGCAGAAAAGGTCCTTATTTCAGCTGAATCTGACACTCAGGGCAACTGGTGCCACGAATATAGGAAACTGAAGGCTAAGGTTGAGACAATACAGAAATGTCAAAAGCACCTCATGGGAGAGGATCTTGAATCTTTGAATCTCAAAGAGCTGCAGCAGCTGGAGCAGCAGCTGGAAAATTCGTTGAAACATATCAGATCCAGAAAGAGCCAACTAATGCTCGAGTCCATTAACGAGCTTCAACGGAAGGAAAAGTCACTGCAGGAGGAGAATAAGGTCCTACAGAAAGAACTGGTGGAGAAGCAGAAAGTCCAGAAGCAACAAGTGCAATGGGACCAGACACAACCTCAAACAAGTTCCTCATCATCCTCCTTCATGATGAGGGAAGCCCTTCCAACAACTAATATCAGTAACTACCCTGCAGCAGCTGGCGAAAGGATAGAGGATGTAGCAGCAGGGCAGCCACAGCATGTTCGCATTGGGCTGCCACCATGGATGCTGAGCCACATCAACGGCTAA
  • >LOC_Os03g54160.1
    ATGGGGCGGGGCAAGGTGCAGCTGAAGCGGATCGAGAACAAGATCAACCGGCAGGTGACCTTCTCCAAGCGCAGGTCGGGGCTGCTCAAGAAGGCGAATGAGATCTCCGTGCTCTGCGACGCCGAGGTCGCGCTCATCATCTTCTCCACCAAGGGCAAGCTCTACGAGTACGCCACCGACTCATGTATGGACAAAATCCTTGAACGTTATGAGCGCTACTCCTATGCAGAAAAGGTCCTTATTTCAGCTGAATCTGACACTCAGGGCAACTGGTGCCACGAATATAGGAAACTGAAGGCTAAGGTTGAGACAATACAGAAATGTCAAAAGCACCTCATGGGAGAGGATCTTGAATCTTTGAATCTCAAAGAGCTGCAGCAGCTGGAGCAGCAGCTGGAAAATTCGTTGAAACATATCAGATCCAGAAAGAGCCAACTAATGCTCGAGTCCATTAACGAGCTTCAACGGAAGGAAAAGTCACTGCAGGAGGAGAATAAGGTCCTACAGAAAGAACTGGTGGAGAAGCAGAAAGTCCAGAAGCAACAAGTGCAATGGGACCAGACACAACCTCAAACAAGTTCCTCATCATCCTCCTTCATGATGAGGGAAGCCCTTCCAACAACTAATATCAGTAACTACCCTGCAGCAGCTGGCGAAAGGATAGAGGATGTAGCAGCAGGGCAGCCACAGCATGTTCGCATTGGGCTGCCACCATGGATGCTGAGCCACATCAACGGCTAA
Protein Sequence
  • >LOC_Os03g54160.2
    MGRGKVQLKRIENKINRQVTFSKRRSGLLKKANEISVLCDAEVALIIFSTKGKLYEYATDSCMDKILERYERYSYAEKVLISAESDTQGNWCHEYRKLKAKVETIQKCQKHLMGEDLESLNLKELQQLEQQLENSLKHIRSRKSQLMLESINELQRKEKSLQEENKVLQKELVEKQKVQKQQVQWDQTQPQTSSSSSSFMMREALPTTNISNYPAAAGERIEDVAAGQPQHVRIGLPPWMLSHING*
  • >LOC_Os03g54160.1
    MGRGKVQLKRIENKINRQVTFSKRRSGLLKKANEISVLCDAEVALIIFSTKGKLYEYATDSCMDKILERYERYSYAEKVLISAESDTQGNWCHEYRKLKAKVETIQKCQKHLMGEDLESLNLKELQQLEQQLENSLKHIRSRKSQLMLESINELQRKEKSLQEENKVLQKELVEKQKVQKQQVQWDQTQPQTSSSSSSFMMREALPTTNISNYPAAAGERIEDVAAGQPQHVRIGLPPWMLSHING*