Information report for OsIAA13;OsiIAA1
Gene Details
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Functional Descriptions
- Earlier, we reported the isolation of OsiIAA1 cDNA, first monocot member of Aux/IAA gene family from rice.
- Extending this work further, we have isolated the OsiIAA1 gene from rice localized on chromosome 3.
- The increased accumulation of OsiIAA1 transcript in auxin-treated rice coleoptiles even in the presence of a protein synthesis inhibitor, cycloheximide, suggested that OsiIAA1 is a primary auxin response gene; the expression of OsiIAA1 gene was also upregulated in the presence of cycloheximide alone.
- The OsiIAA1 transcript levels were down-regulated in etiolated rice coleoptiles irradiated with far-red, red and blue light, suggesting the existence of a cross-talk between auxin and light signaling.
- The antibodies raised against His6-OsiIAA1 recombinant protein could detect the OsiIAA1 protein in the plant extract only in the presence of a proteasome inhibitor, MG132, indicating that OsiIAA1 is rapidly degraded by proteasome complex.
- Also, the proteasome inhibitor MG132 stabilized the purified His6-OsiIAA1 protein to some extent in the cell-free extracts of rice coleoptiles.
- The OsiIAA1 protein harbors two nuclear localization signals (NLSs), one bipartite and the other resembling SV40 type NLS.
- These studies indicate that nuclear localization of OsiIAA1 could be a prerequisite for its role in auxin signal transduction.
- Exogenous auxin enhances the degradation of a light down-regulated and nuclear-localized OsiIAA1, an Aux/IAA protein from rice, via proteasome.
- In this study, we newly isolated and characterized a rice gain-of-function mutant, OsIAA13, containing a single amino acid substitution in the core sequence required for the degradation of the OsIAA13 protein.
- The OsIAA13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective.
- OsIAA13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites.
- Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in OsIAA13.
- Taken together, our results indicate that OsIAA13 is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice.
- OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice.
Functional Keywords
- iaa , root , auxin , lateral-root
Literature and News
- Exogenous auxin enhances the degradation of a light down-regulated and nuclear-localized OsiIAA1, an Aux/IAA protein from rice, via proteasome . DOI: 10.1016/j.bbaexp.2005.08.002 ; PMID: 16139905
- OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice . DOI: 10.1016/j.plantsci.2012.04.005 ; PMID: 22608525
- A comprehensive transcriptome analysis of contrasting rice cultivars highlights the role of auxin and ABA responsive genes in heat stress response . DOI: 10.1016/j.ygeno.2021.03.007 ; PMID: 33705886
- Identification of the OsCML4 Gene in Rice Related to Salt Stress Using QTL Analysis . DOI: 10.3390/plants11192467 ; PMID: 36235331
Gene Resources
- NCBI ID: AJ563599
Sequences
cDNA Sequence
- >LOC_Os03g53150.2
CTTCCCTCTCTCTATATATATACTCATCTCCAAGCTGTTCTTGGCTCCTCTCTCTTCTTCCAACACCTCCTAATCACCACAAAGGCTTAAAAATCTCCAGTCTCCATAGAGAGAAAGAGAGAGAAGAGAAGGCAGCTTGATTTGGGGCGAGGAGACGCGATAGCCGCGATAGGCTAAGCAGCTGGCAGCGGCGGCTGCTGCGATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAGAATGCGGTTTAGCGATACCACGTTCAAGTTTCTCGCATTAGCTGATTGGTTACTCTGCAGGATGTTTGTCGAGTCATGCAAACGCTTGAGGATCATGAAGGGTTCCGAAGCCATTGGTCTTGGTCAGTATCTTCAGCTAAAATTTGCTTTCGTGTCAAGATTTATATCTTAATTCACACCCCTTGTGTGTGTGGTTTTTCTTCCAGCACCAAGAGCAAAGGACAAGTACAAGAACAAGAGCTGAAAATGTTGCAGTATTAAGATGCTGAGCATGGTGCCATAAGAATGGTGTTCTTGCAGTGTGTTATGGTTTCCTTAGAAATATAAGGTTTTACTTGTTTAGCTGGATTAAAAGAAGGAAGAGTTGTCATGTTTAATTTGTTTATGGACCAGAAGCAATAATCTATTTGTGTGTAGCTGGATTGGTGTTATGTGATGTCCCCTACCGTGTGTCCCAAGATCTACTTGATGATTTTATTCCAATGTTGTTAATTTAAGTTTGTGTGATTGTGAAGTAAAGGGTATCAATGAGACTATTATTATTATGGTATCTGGTAAGTTAACATGTTTGTTAATCATCAGCACAATATTTTTGTAA - >LOC_Os03g53150.1
CTTCCCTCTCTCTATATATATACTCATCTCCAAGCTGTTCTTGGCTCCTCTCTCTTCTTCCAACACCTCCTAATCACCACAAAGGCTTAAAAATCTCCAGTCTCCATAGAGAGAAAGAGAGAGAAGAGAAGGCAGCTTGATTTGGGGCGAGGAGACGCGATAGCCGCGATAGGCTAAGCAGCTGGCAGCGGCGGCTGCTGCGATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAGAATGCGGTTTAGCGATACCACGTTCAAGTTTCTCGCATTAGCTGATTGGTTACTCTGCAGGATGTTTGTCGAGTCATGCAAACGCTTGAGGATCATGAAGGGTTCCGAAGCCATTGGTCTTGGTCAGTATCTTCAGCTAAAATTTGCTTTCGTGTCAAGATTTATATCTTAATTCACACCCCTTGTGTGTGTGGTTTTTCTTCCAGCACCAAGAGCAAAGGACAAGTACAAGAACAAGAGCTGAAAATGTTGCAGTATTAAGATGCTGAGCATGGTGCCATAAGAATGGTGTTCTTGCAGTGTGTTATGGTTTCCTTAGAAATATAAGGTTTTACTTGTTTAGCTGGATTAAAAGAAGGAAGAGTTGTCATGTTTAATTTGTTTATGGACCAGAAGCAATAATCTATTTGTGTGTAGCTGGATTGGTGTTATGTGATGTCCCCTACCGTGTGTCCCAAGATCTACTTGATGATTTTATTCCAATGTTGTTAATTTAAGTTTGTGTGATTGTGAAGTAAAGGGTATCAATGAGACTATTATTATTATGGTATCTGGTAAGTTAACATGTTTGTTAATCATCAGCACAATATTTTTGTAA
CDS Sequence
- >LOC_Os03g53150.2
ATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAG - >LOC_Os03g53150.1
ATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAG
Protein Sequence
- >LOC_Os03g53150.2
MAGADVDVGTELRLGLPGGGGGAAEAAAKAAKRGFEETIDLKLKLPTAGMEEAAAGKAEAPAAEKAKRPAEAAAADAEKPPAPKAQAVGWPPVRSFRRNIMTVQSVKSKKEEEADKQQQQPAANASGSNSSAFVKVSMDGAPYLRKVDLKMYNSYKDLSLALQKMFGTFTATGNNMNEVNGSDAVTTYEDKDGDWMLVGDVPWQYVTTSFLCPLNCFPYFPPHD* - >LOC_Os03g53150.1
MAGADVDVGTELRLGLPGGGGGAAEAAAKAAKRGFEETIDLKLKLPTAGMEEAAAGKAEAPAAEKAKRPAEAAAADAEKPPAPKAQAVGWPPVRSFRRNIMTVQSVKSKKEEEADKQQQQPAANASGSNSSAFVKVSMDGAPYLRKVDLKMYNSYKDLSLALQKMFGTFTATGNNMNEVNGSDAVTTYEDKDGDWMLVGDVPWQYVTTSFLCPLNCFPYFPPHD*