Gene Details:

Functional Descriptions:

  • Here, we report the results of a study using a functional genomics approach that identified a rice basic helix-loop-helix domain gene, OsbHLH148, that conferred drought tolerance as a component of the jasmonate signaling module in rice.
  • OsbHLH148 transcript levels were rapidly increased by treatment with methyl jasmonate (MeJA) or abscisic acid, and abiotic stresses including dehydration, high salinity, low temperature and wounding.
  • These results show that OsJAZ1 could act as a transcriptional regulator of the OsbHLH148-related jasmonate signaling pathway leading to drought tolerance.
  • Thus, our study suggests that OsbHLH148 acts on an initial response of jasmonate-regulated gene expression toward drought tolerance, constituting the OsbHLH148-OsJAZ-OsCOI1 signaling module in rice.
  • OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice.
  • Transgenic over-expression of OsbHLH148 in rice confers plant tolerance to drought stress.
  • Yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase assays showed that OsWRKY76 and OsbHLH148 bound directly to the OsDREB1E promoter and activated OsDREB1E expression in response to drought stress.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g53020.1
    GGAAACCATCGCCAACCATCACATCTGATCGAGAACAAGAAGAAGAAGAAGAAGAAGCAAGAACAGCAAGAGGAAGAGATGCAAATGGAGTCGTACTACGGCGCGTTCCACGCCGACGAGGCCGCGTTCTTCTTCCCCCACCACGTGCCGGCGTCGCCGGAGCTGCCGTTCGGCCTCATTGCGTCGCCGGAGCCGGAGCCGGAGCCGGAGCAGGCGGCGGCGGAGGCGAGGCAGAGCGCGTTCCAGGAGTACGGCGGCGCGGTGCACGCCGGAGCGCCGGCGGCGGCGGGGGCTGTCACCACCGGTGGGACGAACATCCACCGGAGGGTGATGGACGTGCTGGGCAGGATGGGCGGCGGCGGCGGCGGGGGGGAGAAGGGGGAGGGTGAGGAGATGGAGGAGGAGGAGGAGGTGCCGCAGCGGCGGCGGCGCGGGCAGGGCGCCGACGTGGAGAGCAGCCGCGGTTTCCGCCACATGATGCGCGAGCGCCAGCGCCGCGAGAAGCTCAGCCAGAGCTACGCCGACCTCTACGCCATGGTCTCCTCTCGCTCCAAGGGGGACAAGAACTCGATCGTGCAGTCGGCGGCGATCTACATCCACGAGCTGAAGGTCGCGAGGGACCAGCTGCAGAGGAGGAACGAGGAGCTGAAGGCCCAGATCATGGGCCACGACGAGCAGCAGCCGTGCGTCACGGTGCAGTTCGAGGTCGACGAGCCGTCGTCGTCGATCGACTCCATGATCGCCGCGCTCCGGCGGCTCAAGGGCATGAGCGTCAAGGCGCGCGGGATCCGGTCGAGCATGTCCGGGAACAGGCTGTGGACGGAGATGAACGTCGAGACCACGATTGCGGCTTGTGAAGTGGAAAAGGCAGTGGAAGAGGCTCTCAAGGAAGTAGAGAGGAACCAGCCTGACAGCGATGCCCCATTTCCTGGAAGCAAAGGCTGGACACAGACATCTCATGTGCAAAATGTGTTTTGATCGGTTTTGAAACATGATCCTGCTGGGACCCCCATTTGTTCATTTTTTTAAAAGCTTGTGATGGAGCAAGTGGAAAAAAAATCTCCATATCTCCTTGTCAATGTACCTTTTTTTTGTCCATGTAGATACAATCATACAAATAATAGCTTCCTCTCTTTTCTACAAGGAAAAAAGAAAGAGAAGAGTACAAAAAAGATTTAAGCAGCTTGTTTATCAGTTAAAAAAGAAAAGGGAATCAGATTTGTCATATCTCAGTCTCTTCCTGTTCCAATCACAAAGGGATCAATTTCACATCCCACATAAACTCA
CDS Sequence
  • >LOC_Os03g53020.1
    ATGCAAATGGAGTCGTACTACGGCGCGTTCCACGCCGACGAGGCCGCGTTCTTCTTCCCCCACCACGTGCCGGCGTCGCCGGAGCTGCCGTTCGGCCTCATTGCGTCGCCGGAGCCGGAGCCGGAGCCGGAGCAGGCGGCGGCGGAGGCGAGGCAGAGCGCGTTCCAGGAGTACGGCGGCGCGGTGCACGCCGGAGCGCCGGCGGCGGCGGGGGCTGTCACCACCGGTGGGACGAACATCCACCGGAGGGTGATGGACGTGCTGGGCAGGATGGGCGGCGGCGGCGGCGGGGGGGAGAAGGGGGAGGGTGAGGAGATGGAGGAGGAGGAGGAGGTGCCGCAGCGGCGGCGGCGCGGGCAGGGCGCCGACGTGGAGAGCAGCCGCGGTTTCCGCCACATGATGCGCGAGCGCCAGCGCCGCGAGAAGCTCAGCCAGAGCTACGCCGACCTCTACGCCATGGTCTCCTCTCGCTCCAAGGGGGACAAGAACTCGATCGTGCAGTCGGCGGCGATCTACATCCACGAGCTGAAGGTCGCGAGGGACCAGCTGCAGAGGAGGAACGAGGAGCTGAAGGCCCAGATCATGGGCCACGACGAGCAGCAGCCGTGCGTCACGGTGCAGTTCGAGGTCGACGAGCCGTCGTCGTCGATCGACTCCATGATCGCCGCGCTCCGGCGGCTCAAGGGCATGAGCGTCAAGGCGCGCGGGATCCGGTCGAGCATGTCCGGGAACAGGCTGTGGACGGAGATGAACGTCGAGACCACGATTGCGGCTTGTGAAGTGGAAAAGGCAGTGGAAGAGGCTCTCAAGGAAGTAGAGAGGAACCAGCCTGACAGCGATGCCCCATTTCCTGGAAGCAAAGGCTGGACACAGACATCTCATGTGCAAAATGTGTTTTGA
Protein Sequence
  • >LOC_Os03g53020.1
    MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPEQAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGEGEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSKGDKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSIDSMIAALRRLKGMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQPDSDAPFPGSKGWTQTSHVQNVF*