Gene Details:

Functional Descriptions:

  • WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice.
  • GORI encodes a seven-WD40-motif protein that interacts with an AP180 N-terminal homology (ANTH)-domain protein, which modulates clathrin-mediated endocytosis (CME), and regulates Rac6 activity in the apical plasma membrane of elongating pollen tubes.
  • Loss of function of GORI or Rac6 reduces pollen germination and tube growth, thereby resulting in male sterility in rice.
  • In contrast, overexpression of Rac6 increases pollen tube elongation, with this effect being rescued by GORI overexpression.
  • Our findings demonstrated that the GORI protein is a positive regulator of pollen germination and tube growth, serving as a link between Rac6 activity regulation and ANTH-mediated endocytosis.
  • Root ion concentrations indicated that OsABT overexpression lines could maintain lower Na(+) and higher K(+)/Na(+) ratios and upregulated expression of salt-related genes OsSOS1 and OsHAK5 compared with the wild-type (WT) Nipponbare plants.
  • A transcriptome analysis revealed that the differentially expressed genes between OsABT overexpression lines and WT plants were enriched in plant hormone signal transduction, including ABA signaling pathway under salt stress.
  • OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage.
  • Thus, OsABT can improve the salt tolerance in rice seedling roots by inhibiting reactive oxygen species accumulation, thereby regulating the intracellular Na(+)/K(+) balance, ABA content, and ABA signaling pathway.
  • Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2.
  • The yeast two-hybrid and bimolecular fluorescence complementation analyses showed that OsABT interacted with the ABA receptor proteins OsPYL4, OsPYL10, and PP2C phosphatase OsABIL2.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g52870.1
    GCAGTTCTCTCTTCTTCCCCCCCTCATCTCCGCCGCCTCGCCGCGAACGGGACGCCGGCCAAGAGCTCGAAGCTGATGAGAGATAGCGACGGCGAAGGGGCCGGCGGCGGCCTCCCGCGCTCGCACCCGTCGAACCTGCCGCTGCCGGCGCCGCACTCCGACCCAAACCTCCAGTTCTCCGGGACGGACGATGACTTCTCCAACCGTCACAGCAGCTCGTCGGCGACGGGCGGCGCGAGCCCCGGGTACTACTCCGACTACCCGTCCAGCTTCAGCGGCGAGTGCTCGCCGTACAACATGTCGCCCTGGAACCAGACCATGGCGTCGCCGTGGTCGCACCACAGCGACGCGTCCATGGCCGGGCTCGGCGGCGCGCCCGCCATGGCGCCCGGGACCAGCCTCATCGGCTCGCTGGTGAGGGAGGAAGGGCACATCTACTCGCTCGCCGCCAAGACCGACACCCTGTACACCGGCTCCGACAGCAAGAACATCCGCGTGTGGCGCAAGCAGAAGGATTCCGGCGGGTTCAAGTCGTCGAGCGGCCTCGTCAAGGCCATCGTCATCTCCGGCGAGCGCATCTTCACCGGGCACCAGGACGGCAAGATCAGGGTGTGGAAGGTGTCGCCCAAGAACGGCCTGCACAAGCGCGTCGGCAGCCTGCCCCGGCTGCGCGACTTCCTCCGCGGCTCGCTCAACCCGTCCAACTACGTCGAGGTCCGCAAGAACCGGACGGCGCTCTGGATCCGCCACAGCGACGCCGTGTCGTGCCTGAGCCCGACGGACTCGGCGCAGGGCCTGCTCTACTCCGGCTCGTGGGACCGGACCTTCAAGGTGTGGCGGATCAACGACTCCAAGTGCCTCGAGTCCGTGGTGGCGCACGACGACAACGTGAACGCCATCGTGGCAGCGTTCGACGGGCTGGTGTTCACCGGGTCGGCGGACGGGACGGTGAAGGTGTGGAAGAGGGAGCTGCAGGGGAAAGGGACCAAGCACGTCGCGGTGCAGACGCTGCTGAAGCAGGAGCACGCGGTGAACGCGCTCGCCGTGAGCGCCGTCGCGCCGGTGCTCTACTGCGGCTCCTCCGACGGGCTCGTCAACTTCTGGGAAGGGGAGCGCCACCTGGTCCACGGCGGCGTGCTGCGCGGGCACAAGAAGGCCGTGTTCTGCCTCGCCGCCGCGGGCTCCCTCCTCCTCAGCGGCTCCGCCGACAACACCATCTACGTGTGGCGGCGCGACGGCGGCGTCCACTCCTGCCTCTCCGTGCTCACCGGCCACACCGAGCCGATCAGGTGCCTCGCCATCGTCGAGGACAACAAGGACAACGCCGCCGTGCCCGTCGACGCCGTGGACAGCAGCTTCGCGTCGGGCTCGTCCACGCGGTGGATTGTGTACAGCGGCAGCCTCGACAAGTCGATCAAGGTGTGGCGCGTCGCCGAGGACGCGCCTGACGCGCTGCTCCGTGGCCCCGGTGGCGGCGACGCGCCGCAGATGTTCGACCGGTACCCCGGCGACCCATTCGGCGCCAGCAGCTCGTCGTTCCGCTAGAACACGCGCCCGATCAACCAATGCACCATTTTGATCAGTTCCAAGAAGAAATTTTCACCTTACAATTGGGCAGATCAACGTCATGAACCTCTCTGATGGCTAACGGTTTGAGCGAATTGAAGGAAATTGCTGGGTTTTGTTTGTTACACGTCCTATATATACTGTGATGAAGACAAGTATGAAATGAATAGCATCAACATCATTTCTGGCCATGAACATCTGAAGCTGCAACGGCAAGTGGTAGAAATGAAGAGCGTATATAGGTAAGTTTGCTTTCAGAAAGTGGCCGGGGGGAATTTTTGATACATGCTCATATATCTGATGTGTACTAGTTGAAGCCGCGACTTGGTATTCATCTTAAGGATCGATTGAAATCAAGATTAATTGGGAACCAATAAAAGGGGATAATTTCAAACTGTTTTTATAGGTTGACGTTAGAGGATTGTTAATTACCCTCTTTGAAGATTAGCACCAAAAAAGTTCATCGGGTGCTTATTCATATGTAGTATTCCCAGTTTATTGTGATGTTCAATGCAAAACTTATGCTCAGTTATGTTTCGATTTGGTTTATCCTTTTTATACATACTTCACACTTCTACTTGTAACTTCATGATGGAGAATGCATGATTCCCTAATGAACAGTCTGTCAGATTTAACA
CDS Sequence
  • >LOC_Os03g52870.1
    ATGAGAGATAGCGACGGCGAAGGGGCCGGCGGCGGCCTCCCGCGCTCGCACCCGTCGAACCTGCCGCTGCCGGCGCCGCACTCCGACCCAAACCTCCAGTTCTCCGGGACGGACGATGACTTCTCCAACCGTCACAGCAGCTCGTCGGCGACGGGCGGCGCGAGCCCCGGGTACTACTCCGACTACCCGTCCAGCTTCAGCGGCGAGTGCTCGCCGTACAACATGTCGCCCTGGAACCAGACCATGGCGTCGCCGTGGTCGCACCACAGCGACGCGTCCATGGCCGGGCTCGGCGGCGCGCCCGCCATGGCGCCCGGGACCAGCCTCATCGGCTCGCTGGTGAGGGAGGAAGGGCACATCTACTCGCTCGCCGCCAAGACCGACACCCTGTACACCGGCTCCGACAGCAAGAACATCCGCGTGTGGCGCAAGCAGAAGGATTCCGGCGGGTTCAAGTCGTCGAGCGGCCTCGTCAAGGCCATCGTCATCTCCGGCGAGCGCATCTTCACCGGGCACCAGGACGGCAAGATCAGGGTGTGGAAGGTGTCGCCCAAGAACGGCCTGCACAAGCGCGTCGGCAGCCTGCCCCGGCTGCGCGACTTCCTCCGCGGCTCGCTCAACCCGTCCAACTACGTCGAGGTCCGCAAGAACCGGACGGCGCTCTGGATCCGCCACAGCGACGCCGTGTCGTGCCTGAGCCCGACGGACTCGGCGCAGGGCCTGCTCTACTCCGGCTCGTGGGACCGGACCTTCAAGGTGTGGCGGATCAACGACTCCAAGTGCCTCGAGTCCGTGGTGGCGCACGACGACAACGTGAACGCCATCGTGGCAGCGTTCGACGGGCTGGTGTTCACCGGGTCGGCGGACGGGACGGTGAAGGTGTGGAAGAGGGAGCTGCAGGGGAAAGGGACCAAGCACGTCGCGGTGCAGACGCTGCTGAAGCAGGAGCACGCGGTGAACGCGCTCGCCGTGAGCGCCGTCGCGCCGGTGCTCTACTGCGGCTCCTCCGACGGGCTCGTCAACTTCTGGGAAGGGGAGCGCCACCTGGTCCACGGCGGCGTGCTGCGCGGGCACAAGAAGGCCGTGTTCTGCCTCGCCGCCGCGGGCTCCCTCCTCCTCAGCGGCTCCGCCGACAACACCATCTACGTGTGGCGGCGCGACGGCGGCGTCCACTCCTGCCTCTCCGTGCTCACCGGCCACACCGAGCCGATCAGGTGCCTCGCCATCGTCGAGGACAACAAGGACAACGCCGCCGTGCCCGTCGACGCCGTGGACAGCAGCTTCGCGTCGGGCTCGTCCACGCGGTGGATTGTGTACAGCGGCAGCCTCGACAAGTCGATCAAGGTGTGGCGCGTCGCCGAGGACGCGCCTGACGCGCTGCTCCGTGGCCCCGGTGGCGGCGACGCGCCGCAGATGTTCGACCGGTACCCCGGCGACCCATTCGGCGCCAGCAGCTCGTCGTTCCGCTAG
Protein Sequence
  • >LOC_Os03g52870.1
    MRDSDGEGAGGGLPRSHPSNLPLPAPHSDPNLQFSGTDDDFSNRHSSSSATGGASPGYYSDYPSSFSGECSPYNMSPWNQTMASPWSHHSDASMAGLGGAPAMAPGTSLIGSLVREEGHIYSLAAKTDTLYTGSDSKNIRVWRKQKDSGGFKSSSGLVKAIVISGERIFTGHQDGKIRVWKVSPKNGLHKRVGSLPRLRDFLRGSLNPSNYVEVRKNRTALWIRHSDAVSCLSPTDSAQGLLYSGSWDRTFKVWRINDSKCLESVVAHDDNVNAIVAAFDGLVFTGSADGTVKVWKRELQGKGTKHVAVQTLLKQEHAVNALAVSAVAPVLYCGSSDGLVNFWEGERHLVHGGVLRGHKKAVFCLAAAGSLLLSGSADNTIYVWRRDGGVHSCLSVLTGHTEPIRCLAIVEDNKDNAAVPVDAVDSSFASGSSTRWIVYSGSLDKSIKVWRVAEDAPDALLRGPGGGDAPQMFDRYPGDPFGASSSSFR*