Information report for GORI;OsABT
Gene Details
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Functional Descriptions
- WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice.
- GORI encodes a seven-WD40-motif protein that interacts with an AP180 N-terminal homology (ANTH)-domain protein, which modulates clathrin-mediated endocytosis (CME), and regulates Rac6 activity in the apical plasma membrane of elongating pollen tubes.
- Loss of function of GORI or Rac6 reduces pollen germination and tube growth, thereby resulting in male sterility in rice.
- In contrast, overexpression of Rac6 increases pollen tube elongation, with this effect being rescued by GORI overexpression.
- Our findings demonstrated that the GORI protein is a positive regulator of pollen germination and tube growth, serving as a link between Rac6 activity regulation and ANTH-mediated endocytosis.
- Root ion concentrations indicated that OsABT overexpression lines could maintain lower Na(+) and higher K(+)/Na(+) ratios and upregulated expression of salt-related genes OsSOS1 and OsHAK5 compared with the wild-type (WT) Nipponbare plants.
- A transcriptome analysis revealed that the differentially expressed genes between OsABT overexpression lines and WT plants were enriched in plant hormone signal transduction, including ABA signaling pathway under salt stress.
- OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage.
- Thus, OsABT can improve the salt tolerance in rice seedling roots by inhibiting reactive oxygen species accumulation, thereby regulating the intracellular Na(+)/K(+) balance, ABA content, and ABA signaling pathway.
- Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2.
- The yeast two-hybrid and bimolecular fluorescence complementation analyses showed that OsABT interacted with the ABA receptor proteins OsPYL4, OsPYL10, and PP2C phosphatase OsABIL2.
Functional Keywords
- growth , pollen , sterility , plasma-membrane , male-sterility , root , stress , seedling , salt , tolerance , ABA , salt-tolerance , salt-stress , R-protein , abscisic-acid , signal-transduction , reactive-oxygen-species , phosphatase
Literature and News
- WD40-domain protein GORI is an integrative scaffold that is required for pollen tube growth in rice . DOI: 10.1080/15592324.2022.2082678 ; PMID: 35642508
- OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage . DOI: 10.3390/ijms231810656 ; PMID: 36142568
Gene Resources
- UniProt: Q10D64
- EMBL: AK111764, AP014959, DP000009
- AlphaFoldDB: Q10D64
- EnsemblPlants: Os03t0738700-01
- Gramene: Os03t0738700-01
- KEGG: dosa:Os03g0738700
- Orthologous matrix: NTIFVWR
- InterPro: IPR001680, IPR015943, IPR020472
- PANTHER: PTHR22844, PTHR22844:SF336
- SUPFAM: SSF50978
- PROSITE: PS50082, PS50294
- Gene3D: 2.130.10.10
- OrthoDB: Q10D64
- SWISS-MODEL: Q10D64
- Conserved Domain Database: cd00200
- eggNOG: KOG4155
Homologs
- Arabidopsis thaliana: AT2G26490, AT3G18950, AT3G49660
- Brassica napus: BnaA06G0270700ZS, BnaC03G0562300ZS
- Brassica oleracea: Bo3g109400
- Brassica rapa: Bra019610
- Nicotiana attenuata: A4A49_08451
- Triticum aestivum: TaWD40-4B.1
Sequences
cDNA Sequence
- >LOC_Os03g52870.1
GCAGTTCTCTCTTCTTCCCCCCCTCATCTCCGCCGCCTCGCCGCGAACGGGACGCCGGCCAAGAGCTCGAAGCTGATGAGAGATAGCGACGGCGAAGGGGCCGGCGGCGGCCTCCCGCGCTCGCACCCGTCGAACCTGCCGCTGCCGGCGCCGCACTCCGACCCAAACCTCCAGTTCTCCGGGACGGACGATGACTTCTCCAACCGTCACAGCAGCTCGTCGGCGACGGGCGGCGCGAGCCCCGGGTACTACTCCGACTACCCGTCCAGCTTCAGCGGCGAGTGCTCGCCGTACAACATGTCGCCCTGGAACCAGACCATGGCGTCGCCGTGGTCGCACCACAGCGACGCGTCCATGGCCGGGCTCGGCGGCGCGCCCGCCATGGCGCCCGGGACCAGCCTCATCGGCTCGCTGGTGAGGGAGGAAGGGCACATCTACTCGCTCGCCGCCAAGACCGACACCCTGTACACCGGCTCCGACAGCAAGAACATCCGCGTGTGGCGCAAGCAGAAGGATTCCGGCGGGTTCAAGTCGTCGAGCGGCCTCGTCAAGGCCATCGTCATCTCCGGCGAGCGCATCTTCACCGGGCACCAGGACGGCAAGATCAGGGTGTGGAAGGTGTCGCCCAAGAACGGCCTGCACAAGCGCGTCGGCAGCCTGCCCCGGCTGCGCGACTTCCTCCGCGGCTCGCTCAACCCGTCCAACTACGTCGAGGTCCGCAAGAACCGGACGGCGCTCTGGATCCGCCACAGCGACGCCGTGTCGTGCCTGAGCCCGACGGACTCGGCGCAGGGCCTGCTCTACTCCGGCTCGTGGGACCGGACCTTCAAGGTGTGGCGGATCAACGACTCCAAGTGCCTCGAGTCCGTGGTGGCGCACGACGACAACGTGAACGCCATCGTGGCAGCGTTCGACGGGCTGGTGTTCACCGGGTCGGCGGACGGGACGGTGAAGGTGTGGAAGAGGGAGCTGCAGGGGAAAGGGACCAAGCACGTCGCGGTGCAGACGCTGCTGAAGCAGGAGCACGCGGTGAACGCGCTCGCCGTGAGCGCCGTCGCGCCGGTGCTCTACTGCGGCTCCTCCGACGGGCTCGTCAACTTCTGGGAAGGGGAGCGCCACCTGGTCCACGGCGGCGTGCTGCGCGGGCACAAGAAGGCCGTGTTCTGCCTCGCCGCCGCGGGCTCCCTCCTCCTCAGCGGCTCCGCCGACAACACCATCTACGTGTGGCGGCGCGACGGCGGCGTCCACTCCTGCCTCTCCGTGCTCACCGGCCACACCGAGCCGATCAGGTGCCTCGCCATCGTCGAGGACAACAAGGACAACGCCGCCGTGCCCGTCGACGCCGTGGACAGCAGCTTCGCGTCGGGCTCGTCCACGCGGTGGATTGTGTACAGCGGCAGCCTCGACAAGTCGATCAAGGTGTGGCGCGTCGCCGAGGACGCGCCTGACGCGCTGCTCCGTGGCCCCGGTGGCGGCGACGCGCCGCAGATGTTCGACCGGTACCCCGGCGACCCATTCGGCGCCAGCAGCTCGTCGTTCCGCTAGAACACGCGCCCGATCAACCAATGCACCATTTTGATCAGTTCCAAGAAGAAATTTTCACCTTACAATTGGGCAGATCAACGTCATGAACCTCTCTGATGGCTAACGGTTTGAGCGAATTGAAGGAAATTGCTGGGTTTTGTTTGTTACACGTCCTATATATACTGTGATGAAGACAAGTATGAAATGAATAGCATCAACATCATTTCTGGCCATGAACATCTGAAGCTGCAACGGCAAGTGGTAGAAATGAAGAGCGTATATAGGTAAGTTTGCTTTCAGAAAGTGGCCGGGGGGAATTTTTGATACATGCTCATATATCTGATGTGTACTAGTTGAAGCCGCGACTTGGTATTCATCTTAAGGATCGATTGAAATCAAGATTAATTGGGAACCAATAAAAGGGGATAATTTCAAACTGTTTTTATAGGTTGACGTTAGAGGATTGTTAATTACCCTCTTTGAAGATTAGCACCAAAAAAGTTCATCGGGTGCTTATTCATATGTAGTATTCCCAGTTTATTGTGATGTTCAATGCAAAACTTATGCTCAGTTATGTTTCGATTTGGTTTATCCTTTTTATACATACTTCACACTTCTACTTGTAACTTCATGATGGAGAATGCATGATTCCCTAATGAACAGTCTGTCAGATTTAACA
CDS Sequence
- >LOC_Os03g52870.1
ATGAGAGATAGCGACGGCGAAGGGGCCGGCGGCGGCCTCCCGCGCTCGCACCCGTCGAACCTGCCGCTGCCGGCGCCGCACTCCGACCCAAACCTCCAGTTCTCCGGGACGGACGATGACTTCTCCAACCGTCACAGCAGCTCGTCGGCGACGGGCGGCGCGAGCCCCGGGTACTACTCCGACTACCCGTCCAGCTTCAGCGGCGAGTGCTCGCCGTACAACATGTCGCCCTGGAACCAGACCATGGCGTCGCCGTGGTCGCACCACAGCGACGCGTCCATGGCCGGGCTCGGCGGCGCGCCCGCCATGGCGCCCGGGACCAGCCTCATCGGCTCGCTGGTGAGGGAGGAAGGGCACATCTACTCGCTCGCCGCCAAGACCGACACCCTGTACACCGGCTCCGACAGCAAGAACATCCGCGTGTGGCGCAAGCAGAAGGATTCCGGCGGGTTCAAGTCGTCGAGCGGCCTCGTCAAGGCCATCGTCATCTCCGGCGAGCGCATCTTCACCGGGCACCAGGACGGCAAGATCAGGGTGTGGAAGGTGTCGCCCAAGAACGGCCTGCACAAGCGCGTCGGCAGCCTGCCCCGGCTGCGCGACTTCCTCCGCGGCTCGCTCAACCCGTCCAACTACGTCGAGGTCCGCAAGAACCGGACGGCGCTCTGGATCCGCCACAGCGACGCCGTGTCGTGCCTGAGCCCGACGGACTCGGCGCAGGGCCTGCTCTACTCCGGCTCGTGGGACCGGACCTTCAAGGTGTGGCGGATCAACGACTCCAAGTGCCTCGAGTCCGTGGTGGCGCACGACGACAACGTGAACGCCATCGTGGCAGCGTTCGACGGGCTGGTGTTCACCGGGTCGGCGGACGGGACGGTGAAGGTGTGGAAGAGGGAGCTGCAGGGGAAAGGGACCAAGCACGTCGCGGTGCAGACGCTGCTGAAGCAGGAGCACGCGGTGAACGCGCTCGCCGTGAGCGCCGTCGCGCCGGTGCTCTACTGCGGCTCCTCCGACGGGCTCGTCAACTTCTGGGAAGGGGAGCGCCACCTGGTCCACGGCGGCGTGCTGCGCGGGCACAAGAAGGCCGTGTTCTGCCTCGCCGCCGCGGGCTCCCTCCTCCTCAGCGGCTCCGCCGACAACACCATCTACGTGTGGCGGCGCGACGGCGGCGTCCACTCCTGCCTCTCCGTGCTCACCGGCCACACCGAGCCGATCAGGTGCCTCGCCATCGTCGAGGACAACAAGGACAACGCCGCCGTGCCCGTCGACGCCGTGGACAGCAGCTTCGCGTCGGGCTCGTCCACGCGGTGGATTGTGTACAGCGGCAGCCTCGACAAGTCGATCAAGGTGTGGCGCGTCGCCGAGGACGCGCCTGACGCGCTGCTCCGTGGCCCCGGTGGCGGCGACGCGCCGCAGATGTTCGACCGGTACCCCGGCGACCCATTCGGCGCCAGCAGCTCGTCGTTCCGCTAG
Protein Sequence
- >LOC_Os03g52870.1
MRDSDGEGAGGGLPRSHPSNLPLPAPHSDPNLQFSGTDDDFSNRHSSSSATGGASPGYYSDYPSSFSGECSPYNMSPWNQTMASPWSHHSDASMAGLGGAPAMAPGTSLIGSLVREEGHIYSLAAKTDTLYTGSDSKNIRVWRKQKDSGGFKSSSGLVKAIVISGERIFTGHQDGKIRVWKVSPKNGLHKRVGSLPRLRDFLRGSLNPSNYVEVRKNRTALWIRHSDAVSCLSPTDSAQGLLYSGSWDRTFKVWRINDSKCLESVVAHDDNVNAIVAAFDGLVFTGSADGTVKVWKRELQGKGTKHVAVQTLLKQEHAVNALAVSAVAPVLYCGSSDGLVNFWEGERHLVHGGVLRGHKKAVFCLAAAGSLLLSGSADNTIYVWRRDGGVHSCLSVLTGHTEPIRCLAIVEDNKDNAAVPVDAVDSSFASGSSTRWIVYSGSLDKSIKVWRVAEDAPDALLRGPGGGDAPQMFDRYPGDPFGASSSSFR*