Gene Details:

Functional Descriptions:

  • To address this issue, we have identified a rice E3-ubiquitin ligase, OsHOS1, and characterized its role in the modulation of the cold stress response.
  • Taken together our results confirm the importance of OsHOS1, and thus of the proteasome, in the modulation of the cold stress signalling in rice.
  • Isolation and characterization of rice (Oryza sativa L.) E3-ubiquitin ligase OsHOS1 gene in the modulation of cold stress response.
  • The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors.
  • The morphological analysis of the roots in transgenic RNAi::OsHOS1 (RNA interference) and wild type (WT) plants, exposed to a mechanical barrier, revealed that the OsHOS1 silencing plants keep a straight root in contrast to WT that exhibit root curling.
  • Using the yeast two-hybrid system and BiFC assays we showed that OsHOS1 interacts with two ERF transcription factors (TFs), OsEREBP1 and OsEREBP2, known to regulate OsRMC gene expression.
  • We showed that OsHOS1 is involved in the regulation of root curling after mechanosensing and that RNAi::OsHOS1 plants failed to exhibit the root curling phenotype observed in WT.
  • Altogether, our results highlight the role of the proteasome modulating plant responses to mechanical stimuli and suggest that OsHOS1 is a hub integrating environmental and hormonal signaling into plant growth and development.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g52700.1
    CCGAAATCCCTAAACCCTATTCCCCACTATCGAGGGCCGCCCGCCTCTCACGCCGCCGTCGTCGTCAACCTCACCGGCCGCCGCCGCCGCCGTCGCGCGTCCTCCCCCATCCCCGCGCGGGCGCCGCAGCGTCCTCCTCCTAGATATCCACCACCTCCGCCGCCCGCCCGCTCCCTCGCCTTCTCCCCCGACTGCTGCTGTTCTTCAAGAGGTTACCATAGCCTGTGATTTGCTGGAGGCCGCGGCCGTCGGCGGAGCAGCCCGACGGCGCAGCGGCGCTGGGAGGTCGACGGAGCAACGGCGAGGTCGCCCAGAGCTCTGCCCGCGTCGACCAATCGGAGATGGAGCAGAATGCTTTGGAGCAGCTGGCATCCATTGATCTCATTGAACTATGCAAGGAGGCTAGAATTGAGCATTGCCGTGCAACTAGGGACTTGAGTAGCTGTGGCCGCTATGTGCAGCATGTGCTTAACTCATGCGGGCATGCATCCTTATGTGCTGAGTGCAGCCAAAGATGTGATGTCTGCCCAATTTGCAGGAGCCCAATACCAGACACTGGGAATAGGGTTCGACTACGCCTTTACTACAAGTGCCTGGAAGCTGGCCTTATCTCTAAGCAGCATGATGAAAGGTTTCAGGAAAAGGAAGACCATAGTGATCCTGTTAACTTGGACGTCCAACGGCTACATTCCTTGTTTGATGTTGCCCTTCAAAACAATTTGGCCTCCTTGATCTGCCACTATACCACAGATGTTTGTTTGGATGAGAATGCTGTGTCAAGTGATCCACTTCTGGCTTTTCTTTTGGATGAGGTGGTCATCAAGGAGTGGTGCAAGAAGGCTAAGTTTGCTGTGCAGTTGGCAGGAATTTCCAGTGTTGTTGAAGTTATGATCACATCTTTTAGTGAAGCAGTTTCAGCTCATGTTAATGACCTTCATCAACTTATGGAAAGCACGCTGAAGGCGAAACAGCATTTGGAGGCCATGATGTGGTGCATCAGGCATAAATTTCTTGAGGACATACCCTCAAGGCACACTAACTTAGCATCTTGGAGTTCTGATGTGATTAAAAGAAAGGCAGATGCAAAAGAACGGAAATGGCCTGAATTCTCTGATAAATCATCTGCGTACAATGAAGCCAACCAGGGGATACTTTTCATTGAGCAGGCACTACAGAACCTTGGGATTCAAGAAAGCAATAGTGGTAGCGAGGAAGGAGTGGAAATTGTCTGTTTGCAGAGTGAACAGTCATCATCTATGTTCTGTTCCACAATTGATCAGTTCAGTGTTGATAAATACCCATTTAAGGACTTGCGAGAGGCAGTTGATGTACTCTTCCTGCATGGATCATCTGATATGGTGATTGCAAAGCAAGCGATTTTCTTGTATTATCTATTTGATCGACACTGGACTAGACCAGATTCAGAATGGAGGTATCTTGTGGATGATTTTGCTGCCACCTTTGGGATTTCTAGAAGAACATTGCTTGAATGTCTGGTATTTTGTCTTTTGGATGACCATTCTTCACATGCTTTGGAGGAGGCATGCTCTCTACTTCCCAAGATTTCTTGCAAGGAAACACATCCGAAGATAGCACAAGTTCTTCTGGAGCGTCATAAACCTGATGTGGCACTGGTTGTGCTAAAGTGCACAGGGCGTGATACCTTCTCTTCTGCTGCTAACATCGAGAAAGATGACACAGAATCTCTTAGTGAGGCAGTGACTGCAGTCCGTGTAAGAATTGAGTATGGCCATCTAACAGAAGCATTTATGTACCACAGGAGCTACTGCTCTAAGGTAAAAGAGCAAAGGTCTGCAGATATGTCACATGTTGAAGATGCTAACAGTTACAAAAGCTCTTGGATGTACCATGTTGAGGTCATGATGACCGAGTTTTGTAATATCTGCATCGAGAGGAACTTTGTTGATAGAATGATTGATCTGCCTTGGGATTCTGAGGAAGGGAAACATCTTCATAAGTCGCTCCTTGACTGTGCTCGTGAAATGCCCATGGAGCCATGTGGTAGTCTACTTGTTGTTTTCTACCTCCAGCGTTATCGGTATTTGGAAGCATATGAGGTCGATCGTAGTCTTCAAAGCTTTGAGCAGAATGCATTGGAAACTGCTAGTGAAGAAAAAGCATCCAAAATCAGAACAATTGCTCAGTGGAGACAAAGTTTGGTTACTAAATGCATTGAGATGCTTCCTGAGGCTGAGAGGGAGGATATGAGAACCATTGGCAGTGCAGAGAGAAACCAATTTGCTGTACAAACTATGCAAAACTCTTCTCCAGCTAATCACATAGTCAAATCACCAAATCCTGTCATTGCATTTAGCTTATCGGCCACCCCTATTCCTCAGAAGAAGTCAAGCCCTCTCCAATCAAGAAATATCAATGTGTTGAATGATTCTGTTGGCCTTAACAGCAGTGCCCGTTCAGAGTTTGGCAGAAAGGTTCCATCTATCCTCCAATGCAGACCAGTTCCCCTGAGTTCACCTATATCCAATGTGAGATCAACTGCAGGGGGTCTATTCCCCTCCATGGGACAGAATGGTGAAGGTCCATATTTAAAGGGGACTAAAGAACTCAGTTTTACGAAAGGAGAGTCTGGTTTAAAAAAGGGAACTAGACCTGCTGGTTATGATTCACTCCCTATGTATTTCAACATGGGTTCTGTTGATACACCTATGAAGGAGTACCGATCAAGTTTGTTGAAGACTGAGGTTAACAAAACCACTCCTTTTCAAGTAAAAGATTCTGTCGGAAAGGGGGAGTTTGACTTCGGTTCACGTGCTGAGAAACCTTTCATCTTAAGTGGCACAGGTGCTGGTCAAAATGGTCACTCAAAGATATCCGATAATGCTGGTTTTCATGAGGTTCACATCCAGAAAACAAAAGTACCTCCTAAAGAGAATGTTCTCAGCTTTGGTAAGAAATCTTCAGTTGATGAAGCACCACCTGGGAAAGGTGTGTCAAGGTGGAGATCCGATGAGTCTAGTGAAGACGAAGATGATAAAAGAACAAGTGGGTATATGGAGAGTGGAGCTTCACTTGCTACTCGAAGACGAGCCAGATTTTCTAGAAGATGATATATTCCATCTGGTCCAGGTGTTTTGATCGCGCGCAGGGGACATCTTGTCGAATTCTTTTCCAGACATAATACTTACACCCCAAATGTAACTGCGATTTGTTTGAGGAAAGGTGCAACTTACCCAATGTCTTGGGGGAAAAATTGTGGCAACTATATTGCAAAATTTTGGTGTGCCATGGCCGCAATGATGTATTGTAACTAAAGTAAATCGATATGTGTGTTGCATCAAAATAGTTTGGCATTTAAAGCTCACAAGTTCTTTATGTTT
CDS Sequence
  • >LOC_Os03g52700.1
    ATGGAGCAGAATGCTTTGGAGCAGCTGGCATCCATTGATCTCATTGAACTATGCAAGGAGGCTAGAATTGAGCATTGCCGTGCAACTAGGGACTTGAGTAGCTGTGGCCGCTATGTGCAGCATGTGCTTAACTCATGCGGGCATGCATCCTTATGTGCTGAGTGCAGCCAAAGATGTGATGTCTGCCCAATTTGCAGGAGCCCAATACCAGACACTGGGAATAGGGTTCGACTACGCCTTTACTACAAGTGCCTGGAAGCTGGCCTTATCTCTAAGCAGCATGATGAAAGGTTTCAGGAAAAGGAAGACCATAGTGATCCTGTTAACTTGGACGTCCAACGGCTACATTCCTTGTTTGATGTTGCCCTTCAAAACAATTTGGCCTCCTTGATCTGCCACTATACCACAGATGTTTGTTTGGATGAGAATGCTGTGTCAAGTGATCCACTTCTGGCTTTTCTTTTGGATGAGGTGGTCATCAAGGAGTGGTGCAAGAAGGCTAAGTTTGCTGTGCAGTTGGCAGGAATTTCCAGTGTTGTTGAAGTTATGATCACATCTTTTAGTGAAGCAGTTTCAGCTCATGTTAATGACCTTCATCAACTTATGGAAAGCACGCTGAAGGCGAAACAGCATTTGGAGGCCATGATGTGGTGCATCAGGCATAAATTTCTTGAGGACATACCCTCAAGGCACACTAACTTAGCATCTTGGAGTTCTGATGTGATTAAAAGAAAGGCAGATGCAAAAGAACGGAAATGGCCTGAATTCTCTGATAAATCATCTGCGTACAATGAAGCCAACCAGGGGATACTTTTCATTGAGCAGGCACTACAGAACCTTGGGATTCAAGAAAGCAATAGTGGTAGCGAGGAAGGAGTGGAAATTGTCTGTTTGCAGAGTGAACAGTCATCATCTATGTTCTGTTCCACAATTGATCAGTTCAGTGTTGATAAATACCCATTTAAGGACTTGCGAGAGGCAGTTGATGTACTCTTCCTGCATGGATCATCTGATATGGTGATTGCAAAGCAAGCGATTTTCTTGTATTATCTATTTGATCGACACTGGACTAGACCAGATTCAGAATGGAGGTATCTTGTGGATGATTTTGCTGCCACCTTTGGGATTTCTAGAAGAACATTGCTTGAATGTCTGGTATTTTGTCTTTTGGATGACCATTCTTCACATGCTTTGGAGGAGGCATGCTCTCTACTTCCCAAGATTTCTTGCAAGGAAACACATCCGAAGATAGCACAAGTTCTTCTGGAGCGTCATAAACCTGATGTGGCACTGGTTGTGCTAAAGTGCACAGGGCGTGATACCTTCTCTTCTGCTGCTAACATCGAGAAAGATGACACAGAATCTCTTAGTGAGGCAGTGACTGCAGTCCGTGTAAGAATTGAGTATGGCCATCTAACAGAAGCATTTATGTACCACAGGAGCTACTGCTCTAAGGTAAAAGAGCAAAGGTCTGCAGATATGTCACATGTTGAAGATGCTAACAGTTACAAAAGCTCTTGGATGTACCATGTTGAGGTCATGATGACCGAGTTTTGTAATATCTGCATCGAGAGGAACTTTGTTGATAGAATGATTGATCTGCCTTGGGATTCTGAGGAAGGGAAACATCTTCATAAGTCGCTCCTTGACTGTGCTCGTGAAATGCCCATGGAGCCATGTGGTAGTCTACTTGTTGTTTTCTACCTCCAGCGTTATCGGTATTTGGAAGCATATGAGGTCGATCGTAGTCTTCAAAGCTTTGAGCAGAATGCATTGGAAACTGCTAGTGAAGAAAAAGCATCCAAAATCAGAACAATTGCTCAGTGGAGACAAAGTTTGGTTACTAAATGCATTGAGATGCTTCCTGAGGCTGAGAGGGAGGATATGAGAACCATTGGCAGTGCAGAGAGAAACCAATTTGCTGTACAAACTATGCAAAACTCTTCTCCAGCTAATCACATAGTCAAATCACCAAATCCTGTCATTGCATTTAGCTTATCGGCCACCCCTATTCCTCAGAAGAAGTCAAGCCCTCTCCAATCAAGAAATATCAATGTGTTGAATGATTCTGTTGGCCTTAACAGCAGTGCCCGTTCAGAGTTTGGCAGAAAGGTTCCATCTATCCTCCAATGCAGACCAGTTCCCCTGAGTTCACCTATATCCAATGTGAGATCAACTGCAGGGGGTCTATTCCCCTCCATGGGACAGAATGGTGAAGGTCCATATTTAAAGGGGACTAAAGAACTCAGTTTTACGAAAGGAGAGTCTGGTTTAAAAAAGGGAACTAGACCTGCTGGTTATGATTCACTCCCTATGTATTTCAACATGGGTTCTGTTGATACACCTATGAAGGAGTACCGATCAAGTTTGTTGAAGACTGAGGTTAACAAAACCACTCCTTTTCAAGTAAAAGATTCTGTCGGAAAGGGGGAGTTTGACTTCGGTTCACGTGCTGAGAAACCTTTCATCTTAAGTGGCACAGGTGCTGGTCAAAATGGTCACTCAAAGATATCCGATAATGCTGGTTTTCATGAGGTTCACATCCAGAAAACAAAAGTACCTCCTAAAGAGAATGTTCTCAGCTTTGGTAAGAAATCTTCAGTTGATGAAGCACCACCTGGGAAAGGTGTGTCAAGGTGGAGATCCGATGAGTCTAGTGAAGACGAAGATGATAAAAGAACAAGTGGGTATATGGAGAGTGGAGCTTCACTTGCTACTCGAAGACGAGCCAGATTTTCTAGAAGATGA
Protein Sequence
  • >LOC_Os03g52700.1
    MEQNALEQLASIDLIELCKEARIEHCRATRDLSSCGRYVQHVLNSCGHASLCAECSQRCDVCPICRSPIPDTGNRVRLRLYYKCLEAGLISKQHDERFQEKEDHSDPVNLDVQRLHSLFDVALQNNLASLICHYTTDVCLDENAVSSDPLLAFLLDEVVIKEWCKKAKFAVQLAGISSVVEVMITSFSEAVSAHVNDLHQLMESTLKAKQHLEAMMWCIRHKFLEDIPSRHTNLASWSSDVIKRKADAKERKWPEFSDKSSAYNEANQGILFIEQALQNLGIQESNSGSEEGVEIVCLQSEQSSSMFCSTIDQFSVDKYPFKDLREAVDVLFLHGSSDMVIAKQAIFLYYLFDRHWTRPDSEWRYLVDDFAATFGISRRTLLECLVFCLLDDHSSHALEEACSLLPKISCKETHPKIAQVLLERHKPDVALVVLKCTGRDTFSSAANIEKDDTESLSEAVTAVRVRIEYGHLTEAFMYHRSYCSKVKEQRSADMSHVEDANSYKSSWMYHVEVMMTEFCNICIERNFVDRMIDLPWDSEEGKHLHKSLLDCAREMPMEPCGSLLVVFYLQRYRYLEAYEVDRSLQSFEQNALETASEEKASKIRTIAQWRQSLVTKCIEMLPEAEREDMRTIGSAERNQFAVQTMQNSSPANHIVKSPNPVIAFSLSATPIPQKKSSPLQSRNINVLNDSVGLNSSARSEFGRKVPSILQCRPVPLSSPISNVRSTAGGLFPSMGQNGEGPYLKGTKELSFTKGESGLKKGTRPAGYDSLPMYFNMGSVDTPMKEYRSSLLKTEVNKTTPFQVKDSVGKGEFDFGSRAEKPFILSGTGAGQNGHSKISDNAGFHEVHIQKTKVPPKENVLSFGKKSSVDEAPPGKGVSRWRSDESSEDEDDKRTSGYMESGASLATRRRARFSRR*