Gene Details:

Functional Descriptions:

  • The upregulation of OsGRF6 results in the coordinated activation of several immediate downstream biological clades, including auxin (IAA) biosynthesis, auxin response factors, and branch and spikelet development-related transcription factors.
  • When rice seedlings were subjected to chilling stress, the repressive effect of OsGRF6 on OsGA2ox1 was released by cold-induced SLR1, which activated OsGA2ox1 expression to decrease the active GA levels, enhancing chilling tolerance.
  • OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice.
  • These results suggest that OsGRF6 is an important regulator in the balance between growth and chilling tolerance in rice.
  • After cold treatment and recovery, the survival rates of OsGRF6-overexpression lines and an OsGRF6 mutant and its complementary lines indicated that OsGRF6 is a negative regulator of chilling tolerance in rice.
  • The yeast one-hybrid, qRT-PCR, and transactivation assays showed that both SLR1 and OsGRF6 can bind to the promoter of the active GA catabolic gene OsGA2ox1, where SLR1 promoted and OsGRF6 suppressed OsGA2ox1 expression.
  • At normal temperature, OsGRF6 was responsible for maintaining active GA levels by inhibiting OsGA2ox1.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g51970.1
    GTTGGCTTGCACTGCCTAGCTTGTTGCTCATCTCCTTGGTGTATCTGGCTGCAGGATTGAGCTCAGTGAGCTTGAGCAGCAGCATGCAGGGTGCAATGGCCAGGGTGAGGGGTCCCTTCACGCCGTCTCAGTGGATCGAGCTGGAGCACCAGGCGCTGATATACAAGTACTTGGCTGCGAATAGCCCTGTACCACACAGCCTCCTCATCCCCATCAGGAGGAGCCTCACATCGCCCTACTCACCTGCCTACTTTGGCTCAAGCACATTGGGATGGGGATCTTTCCAGCTGGGCTACTCCGGCAGCGCGGATCCGGAGCCCGGCCGGTGCCGCCGGACGGACGGCAAGAAATGGCGGTGCTCGAGGGATGCGGTCGCCGACCAGAAGTACTGTGAGCGACACATGAACCGGGGACGCCACCGTTCAAGAAAGCATGTGGAAGGCCAGCCTGGCCATGCCGCGAAAGCGATGCCCGCGGCGGTGGCAGCAGCCGCTGCCTCTGCTACCCAGCCTAGTGCTCCGGCCGCCCACAGTGGCGGAGCTGTTGCTGGCCTCGCTATCAACCATCAGCACCAGCAAATGAAGAACTACGCTGCCAACACTGCCAATCCTTGCTCTCTGCAATATAGCAGGGATCTGGCAAACAAGCATAATGAGAGTGAACAAGTGCAAGACTCAGACAGTCTCTCGATGCTGACTTCCATTAGCACGAGAAATACGGGCAGCCTGTTTCCGTTCTCAAAACAACATAATCCTTTTGAAGTGTCCAACTCAAGGCCAGATTTTGGCCTAGTATCACCTGATTCACTGATGAGTTCTCCTCATAGCTCCTTGGAGAACGTCAATTTGCTCACTTCGCAGAGTCTGAATGAACAACAGAGTTCAGTTTCCCTTCAACACTTTGTGGACTGGCCAAGGACACCTGCACAAGGAGCTCTCGCATGGCCTGATGCTGAAGACATGCAAGCTCAGAGAAGCCAGCTCTCAATATCTGCTCCAATGGCGTCTTCTGACCTGTCATCAGCCTCAACATCTCCCATCCATGAGAAGCTGATGTTGTCACCACTTAAACTGAGCCGTGAATATAGTCCTATTGGTCTCGGTTTTGCAGCAAATAGAGATGAGGTTAACCAGGGAGAAGCAAACTGGATGCCTATGTTCCGTGATTCTTTGATGGGCGGACCATTGGGAGAGGTTTTAACCAAGAATAACAACATGGAAGCAAGGAATTGCCTATCGGAGTCTCTGAATCTTTTAAATGATGGCTGGGATTCAAGCTCAGGGTTTGATTCATCCCCAGTTGGTGTTCTGCAGAAGACCACCTTTGGATCAGTATCCAGTAGCACCGGAAGCAGTCCTAGACTGGAGAATCATAGTGTTTATGATGGCAACAGTAACCTGCGGGATGATCTCGGTTCAGTTGTTGTAAATCATCCGAGCATCCGCCTGGTGTGAGCTTCTTGATCAACAAAAAATGCAACAAGATGACAGGCATGAGGAAACGATTCAATCAGTCTTCACCTTGTTACATATCGTTATGACGTCTTCAAGTGTGAGCATTTACCTCGGTGCTTTGTCATTCAGACTAATCTTGTGTTTTTGGTATTTCTGCTCTGTTATTTTTCCTTTGCTTTGTAACCTTCACCTTGGATCTTGACGTGATATGACGTGTGGTATGGATGGATTTGGTACTCCATTGTGGCATATCATCTTAAAATATCCCATGCTGTGACGTCATAATCGCGGATGAATGTAATCCGATCCTTGTCTTGCTTTCTGGTAGTATTCAATGGATTGTTTGTGAGCTGATAT
CDS Sequence
  • >LOC_Os03g51970.1
    ATGCAGGGTGCAATGGCCAGGGTGAGGGGTCCCTTCACGCCGTCTCAGTGGATCGAGCTGGAGCACCAGGCGCTGATATACAAGTACTTGGCTGCGAATAGCCCTGTACCACACAGCCTCCTCATCCCCATCAGGAGGAGCCTCACATCGCCCTACTCACCTGCCTACTTTGGCTCAAGCACATTGGGATGGGGATCTTTCCAGCTGGGCTACTCCGGCAGCGCGGATCCGGAGCCCGGCCGGTGCCGCCGGACGGACGGCAAGAAATGGCGGTGCTCGAGGGATGCGGTCGCCGACCAGAAGTACTGTGAGCGACACATGAACCGGGGACGCCACCGTTCAAGAAAGCATGTGGAAGGCCAGCCTGGCCATGCCGCGAAAGCGATGCCCGCGGCGGTGGCAGCAGCCGCTGCCTCTGCTACCCAGCCTAGTGCTCCGGCCGCCCACAGTGGCGGAGCTGTTGCTGGCCTCGCTATCAACCATCAGCACCAGCAAATGAAGAACTACGCTGCCAACACTGCCAATCCTTGCTCTCTGCAATATAGCAGGGATCTGGCAAACAAGCATAATGAGAGTGAACAAGTGCAAGACTCAGACAGTCTCTCGATGCTGACTTCCATTAGCACGAGAAATACGGGCAGCCTGTTTCCGTTCTCAAAACAACATAATCCTTTTGAAGTGTCCAACTCAAGGCCAGATTTTGGCCTAGTATCACCTGATTCACTGATGAGTTCTCCTCATAGCTCCTTGGAGAACGTCAATTTGCTCACTTCGCAGAGTCTGAATGAACAACAGAGTTCAGTTTCCCTTCAACACTTTGTGGACTGGCCAAGGACACCTGCACAAGGAGCTCTCGCATGGCCTGATGCTGAAGACATGCAAGCTCAGAGAAGCCAGCTCTCAATATCTGCTCCAATGGCGTCTTCTGACCTGTCATCAGCCTCAACATCTCCCATCCATGAGAAGCTGATGTTGTCACCACTTAAACTGAGCCGTGAATATAGTCCTATTGGTCTCGGTTTTGCAGCAAATAGAGATGAGGTTAACCAGGGAGAAGCAAACTGGATGCCTATGTTCCGTGATTCTTTGATGGGCGGACCATTGGGAGAGGTTTTAACCAAGAATAACAACATGGAAGCAAGGAATTGCCTATCGGAGTCTCTGAATCTTTTAAATGATGGCTGGGATTCAAGCTCAGGGTTTGATTCATCCCCAGTTGGTGTTCTGCAGAAGACCACCTTTGGATCAGTATCCAGTAGCACCGGAAGCAGTCCTAGACTGGAGAATCATAGTGTTTATGATGGCAACAGTAACCTGCGGGATGATCTCGGTTCAGTTGTTGTAAATCATCCGAGCATCCGCCTGGTGTGA
Protein Sequence
  • >LOC_Os03g51970.1
    MQGAMARVRGPFTPSQWIELEHQALIYKYLAANSPVPHSLLIPIRRSLTSPYSPAYFGSSTLGWGSFQLGYSGSADPEPGRCRRTDGKKWRCSRDAVADQKYCERHMNRGRHRSRKHVEGQPGHAAKAMPAAVAAAAASATQPSAPAAHSGGAVAGLAINHQHQQMKNYAANTANPCSLQYSRDLANKHNESEQVQDSDSLSMLTSISTRNTGSLFPFSKQHNPFEVSNSRPDFGLVSPDSLMSSPHSSLENVNLLTSQSLNEQQSSVSLQHFVDWPRTPAQGALAWPDAEDMQAQRSQLSISAPMASSDLSSASTSPIHEKLMLSPLKLSREYSPIGLGFAANRDEVNQGEANWMPMFRDSLMGGPLGEVLTKNNNMEARNCLSESLNLLNDGWDSSSGFDSSPVGVLQKTTFGSVSSSTGSSPRLENHSVYDGNSNLRDDLGSVVVNHPSIRLV*