Information report for OsGRAS19;OsSCL7
Gene Details
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Functional Descriptions
- OsGRAS19 may be a novel component involved in the brassinosteroid signaling pathway in rice.
- Novel OsGRAS19 mutant, D26, positively regulates grain shape in rice (Oryza sativa).
- Gene D26 is a novel site mutation of OsGRAS19 and involved in the brassinosteroid (BR) signalling pathway.
- We characterized the function of OsSCL7 in rice disease resistance.
- oryzae and pathogen-associated molecular pattern treatments, and knocking out OsSCL7 resulted in decreased disease resistance of rice to M.
- In contrast, overexpression of OsSCL7 could improve rice disease resistance to M.
- The 14-3-3 protein GF14c positively regulates immunity by modulating the protein homoeostasis of the GRAS protein OsSCL7 in rice.
- Taken together, these findings uncover the important roles of OsSCL7 and GF14c in plant immunity and a potential mechanism by which plants fine-tune immunity by regulating the protein stability of a GRAS protein via a 14-3-3 protein.
- OsSCL7 was mainly localized in the nucleus and showed transcriptional activity.
- Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation.
- Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size.
- CONCLUSION: WG3 functions directly in regulating grain size and BR signaling in rice.
- Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice.
Functional Keywords
- brassinosteroid , grain , Brassinosteroid , resistance , disease , disease-resistance , immunity , nucleus , grain-size , BR , BR-signaling , dwarf , cell-proliferation
Literature and News
- OsGRAS19 may be a novel component involved in the brassinosteroid signaling pathway in rice . DOI: 10.1093/mp/sst027 ; PMID: 23389891
- Novel OsGRAS19 mutant, D26, positively regulates grain shape in rice (Oryza sativa) . DOI: 10.1071/FP18266 ; PMID: 31146805
- The 14-3-3 protein GF14c positively regulates immunity by modulating the protein homoeostasis of the GRAS protein OsSCL7 in rice . DOI: 10.1111/pce.14278 ; PMID: 35129212
- Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice . DOI: 10.1186/s12284-022-00601-4 ; PMID: 36326916
Gene Resources
- UniProt: Q53K16
- EMBL: AC146936, AK064022, AK101603
- AlphaFoldDB: Q53K16
- EnsemblPlants: Os03t0723000-01, Os03t0723000-02
- Gramene: Os03t0723000-01, Os03t0723000-02
- KEGG: dosa:Os03g0723000
- Orthologous matrix: EPNMTRD
Homologs
- Brachypodium distachyon: Bradi1g10330.1.p
- Panicum virgatum: Pavir.9NG126900.1.p
- Setaria italica: Seita.9G112200.1.p
- Setaria viridis: Sevir.9G110700.1.p
- Triticum aestivum: Traes_4BS_DCA6E2BE0.2
- Zea mays: GRMZM2G133169_P01, GRMZM2G104342_P01
Sequences
cDNA Sequence
- >LOC_Os03g51330.1
GTTCATCACTCCCTCCCCATTTTACCGTTGAGGTCGGCGACCTCCGCGGACGGTACGGCGGGACACTAGCCCGTCCCGAGGGGCAATCCCGTAATTTTCGCGCAGTACAGTGCCTCCCAGCGCAGTGGCTTCGCCCCACGCCCTACTGTTTTAAATCCCCCTGCCGCTCTCCTCCGCCGTCTTCTTCCCCCCCTTCCTCTCCACCACCCCCGATTCGTCGTCCACCACCGTTCGACGAGTTTCTCGCAGATCGGGCGGGGGCGCGCCGGGATGGCGTACATGTGCGCGGACAGCGGCAACCTCATGGCCATCGCGCAGCAGGTGATCCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGAGGCACCACCACCACCACCATCTGCCGCCGCCGCCGCCGCCGCAGTCGATGGCGCCGCACCACCACCAGCAGAAGCACCACCACCACCACCAGCAGATGCCGGCGATGCCGCAGGCGCCGCCCTCGTCGCACGGCCAGATCCCGGGCCAGCTCGCGTACGGTGGCGGCGCGGCGTGGCCCGCCGGGGAGCACTTCTTCGCGGACGCGTTCGGGGCGTCCGCCGGCGACGCGGTGTTCTCCGACCTCGCGGCGGCCGCGGACTTCGACTCTGATGGGTGGATGGAGAGCCTTATTGGGGACGCCCCCTTCCAGGACTCCGACCTGGAACGCCTCATCTTCACCACCCCGCCGCCGCCCGTGCCGTCCCCACCCCCCACGCATGCCGCCGCCACCGCCACCGCCACCGCCGCCACCGCCGCTCCTCGACCTGAGGCTGCACCGGCTTTGCTCCCCCAGCCTGCTGCTGCGACCCCGGTGGCGTGTTCGTCTCCGAGTCCGAGTTCCGCGGACGCCTCCTGCTCCGCTCCCATCCTCCAGTCCCTCCTGTCCTGCTCCCGCGCGGCGGCGACCGACCCTGGCCTCGCCGCCGCGGAGCTCGCCAGCGTCCGCGCCGCCGCGACTGACGCCGGGGACCCATCTGAGCGCTTGGCCTTCTACTTCGCTGACGCGCTTAGTCGCCGCCTTGCGTGCGGCACCGGAGCTCCGCCCTCCGCGGAGCCGGACGCGCGGTTCGCCTCCGACGAGCTCACGCTCTGCTACAAGACGCTCAACGACGCCTGCCCCTACTCCAAGTTCGCGCACCTCACCGCCAACCAGGCCATCCTGGAGGCCACAGGCGCGGCGACCAAGATCCACATAGTTGACTTCGGGATCGTGCAGGGCATCCAGTGGGCGGCGCTGCTTCAGGCGCTCGCCACCCGGCCCGAGGGGAAGCCAACGCGGATACGGATCACCGGCGTCCCCTCGCCGCTCCTGGGGCCGCAGCCCGCGGCGTCCCTCGCCGCCACCAATACCCGTCTCCGTGACTTCGCCAAGCTCCTCGGGGTGGACTTCGAGTTCGTCCCATTGCTCCGGCCTGTGCACGAGCTCAACAAGTCGGATTTCTTGGTTGAACCTGACGAGGCCGTGGCCGTCAATTTCATGCTGCAGCTGTACCACCTTCTTGGCGACTCGGACGAGCTAGTGAGAAGAGTGCTTCGTCTTGCCAAATCGCTCAGTCCAGCAGTGGTCACACTTGGAGAGTACGAGGTCAGCCTGAACCGAGCTGGGTTCGTCGACCGGTTCGCCAATGCGTTGAGCTATTACCGGTCATTGTTCGAGTCGCTGGATGTGGCAATGACCCGGGACTCACCGGAGAGGGTGAGGGTGGAGCGTTGGATGTTCGGGGAGCGAATTCAGAGGGCCGTCGGGCCGGAGGAAGGCGCAGACCGGACGGAGAGGATGGCCGGCAGCAGCGAGTGGCAGACCCTGATGGAGTGGTGCGGCTTCGAGCCCGTGCCGCTCAGCAACTATGCCAGGAGCCAGGCTGATCTTTTGCTCTGGAACTATGATTCCAAGTACAAGTATTCACTTGTTGAGTTGCCACCGGCGTTCTTGTCCTTGGCATGGGAGAAACGGCCATTGCTTACTGTGTCTGCTTGGAGGTGAGGGTAGTTTTTTTGGGAGTATTTGGGGGAATGTGTGGCGTTGTGATAGAACCTGGGAGGAAATAGTTAGGAGAAATCATTTTGCAGCTTTTGTTGTAGTTTCCTCCAAAACTGTGTTTAGGATGATTGGTATAATTGGGAAATTTTAGCTGAATTATCTTGCTCGGTGTTTGTGCTGCTGAATGTGTGCATGATATTAACTGGTTAGAGAAAAATTAGTAGTAGTTGTACAGAATTTGGTAACAGGTTTTAGCTGTGGTATATTGCTTGCTCATGTTACCAGGATGAGAAATATAAATCTGGATGCAATTGTGCATTTTAAGTAACAGAATCCAACCACAAGCAATATTTAAGTAATTTTCAGCTCCTGATATGATACTTTTTTGAACATCTTGTATATTCATAATCCAACATTTCAGTCCATTTATTTCTTTTGAAAGCATCTGATTGTATCAAAGTGATCTCTGTAGTTATGCATGTACTCCTGGTTTGTCCCTTCAGTAATGAAGTGTTGTCTGGAACTGGAAAGACCTCTTCGACT
CDS Sequence
- >LOC_Os03g51330.1
ATGGCGTACATGTGCGCGGACAGCGGCAACCTCATGGCCATCGCGCAGCAGGTGATCCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGAGGCACCACCACCACCACCATCTGCCGCCGCCGCCGCCGCCGCAGTCGATGGCGCCGCACCACCACCAGCAGAAGCACCACCACCACCACCAGCAGATGCCGGCGATGCCGCAGGCGCCGCCCTCGTCGCACGGCCAGATCCCGGGCCAGCTCGCGTACGGTGGCGGCGCGGCGTGGCCCGCCGGGGAGCACTTCTTCGCGGACGCGTTCGGGGCGTCCGCCGGCGACGCGGTGTTCTCCGACCTCGCGGCGGCCGCGGACTTCGACTCTGATGGGTGGATGGAGAGCCTTATTGGGGACGCCCCCTTCCAGGACTCCGACCTGGAACGCCTCATCTTCACCACCCCGCCGCCGCCCGTGCCGTCCCCACCCCCCACGCATGCCGCCGCCACCGCCACCGCCACCGCCGCCACCGCCGCTCCTCGACCTGAGGCTGCACCGGCTTTGCTCCCCCAGCCTGCTGCTGCGACCCCGGTGGCGTGTTCGTCTCCGAGTCCGAGTTCCGCGGACGCCTCCTGCTCCGCTCCCATCCTCCAGTCCCTCCTGTCCTGCTCCCGCGCGGCGGCGACCGACCCTGGCCTCGCCGCCGCGGAGCTCGCCAGCGTCCGCGCCGCCGCGACTGACGCCGGGGACCCATCTGAGCGCTTGGCCTTCTACTTCGCTGACGCGCTTAGTCGCCGCCTTGCGTGCGGCACCGGAGCTCCGCCCTCCGCGGAGCCGGACGCGCGGTTCGCCTCCGACGAGCTCACGCTCTGCTACAAGACGCTCAACGACGCCTGCCCCTACTCCAAGTTCGCGCACCTCACCGCCAACCAGGCCATCCTGGAGGCCACAGGCGCGGCGACCAAGATCCACATAGTTGACTTCGGGATCGTGCAGGGCATCCAGTGGGCGGCGCTGCTTCAGGCGCTCGCCACCCGGCCCGAGGGGAAGCCAACGCGGATACGGATCACCGGCGTCCCCTCGCCGCTCCTGGGGCCGCAGCCCGCGGCGTCCCTCGCCGCCACCAATACCCGTCTCCGTGACTTCGCCAAGCTCCTCGGGGTGGACTTCGAGTTCGTCCCATTGCTCCGGCCTGTGCACGAGCTCAACAAGTCGGATTTCTTGGTTGAACCTGACGAGGCCGTGGCCGTCAATTTCATGCTGCAGCTGTACCACCTTCTTGGCGACTCGGACGAGCTAGTGAGAAGAGTGCTTCGTCTTGCCAAATCGCTCAGTCCAGCAGTGGTCACACTTGGAGAGTACGAGGTCAGCCTGAACCGAGCTGGGTTCGTCGACCGGTTCGCCAATGCGTTGAGCTATTACCGGTCATTGTTCGAGTCGCTGGATGTGGCAATGACCCGGGACTCACCGGAGAGGGTGAGGGTGGAGCGTTGGATGTTCGGGGAGCGAATTCAGAGGGCCGTCGGGCCGGAGGAAGGCGCAGACCGGACGGAGAGGATGGCCGGCAGCAGCGAGTGGCAGACCCTGATGGAGTGGTGCGGCTTCGAGCCCGTGCCGCTCAGCAACTATGCCAGGAGCCAGGCTGATCTTTTGCTCTGGAACTATGATTCCAAGTACAAGTATTCACTTGTTGAGTTGCCACCGGCGTTCTTGTCCTTGGCATGGGAGAAACGGCCATTGCTTACTGTGTCTGCTTGGAGGTGA
Protein Sequence
- >LOC_Os03g51330.1
MAYMCADSGNLMAIAQQVIQQQQQQQQQQQRHHHHHHLPPPPPPQSMAPHHHQQKHHHHHQQMPAMPQAPPSSHGQIPGQLAYGGGAAWPAGEHFFADAFGASAGDAVFSDLAAAADFDSDGWMESLIGDAPFQDSDLERLIFTTPPPPVPSPPPTHAAATATATAATAAPRPEAAPALLPQPAAATPVACSSPSPSSADASCSAPILQSLLSCSRAAATDPGLAAAELASVRAAATDAGDPSERLAFYFADALSRRLACGTGAPPSAEPDARFASDELTLCYKTLNDACPYSKFAHLTANQAILEATGAATKIHIVDFGIVQGIQWAALLQALATRPEGKPTRIRITGVPSPLLGPQPAASLAATNTRLRDFAKLLGVDFEFVPLLRPVHELNKSDFLVEPDEAVAVNFMLQLYHLLGDSDELVRRVLRLAKSLSPAVVTLGEYEVSLNRAGFVDRFANALSYYRSLFESLDVAMTRDSPERVRVERWMFGERIQRAVGPEEGADRTERMAGSSEWQTLMEWCGFEPVPLSNYARSQADLLLWNYDSKYKYSLVELPPAFLSLAWEKRPLLTVSAWR*