Gene Details:

Functional Descriptions:

  • The knock down or knock out of OsUCL8 also increases grain yield, while the overexpression of OsUCL8 results in an opposite phenotype.
  • Further studies revealed that the cleavage of OsUCL8 by miR408 affects copper homeostasis in the plant cell, which in turn affects the abundance of plastocyanin proteins and photosynthesis in rice.
  • Spatial and temporal expression analyses showed that OsUCL8 was highly expressed in pistils, young panicles, developing seeds and inflorescence meristem, and was nearly complementary to that of OsmiR408.
  • Interestingly, the OsUCL8 protein was localized to the cytoplasm, distinctive from a majority of phytocyanins which localize to the plasma membrane.
  • The overexpression of OsUCL8 led to a striking irregularity in pollen tube growth and pollination and thus affected the seed setting rate in rice; many pollen tubes appeared to lose the ability to grow directly into the style.
  • The rice plantacyanin family member OsUCL8 plays an important role in pollen tube formation and growth and, in turn, regulates fertility and the seed setting rate.
  • We further demonstrated that OsUCL8 mainly affects pollen intine formation.
  • The addition of Vitamin B1 (VB1) significantly contributed to the germination of OXUCL8 pollen grains, suggesting that OsUCL8 could be associated with VB1 production.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g50140.1
    GGCATTGGCAACCAGCCAGCCTCCAGCTACACACTGACACTGCACTCCAGCCTCCGTAGGAGTACTTCAGCTCACTATAGAATGGCAACGGCAATGGCATCGTTACCTCCAGGCCCCAGCTAGTGCAGAGATTTCTCTTCCTTTGCCCAGTTTCCAGGCAGAAGCATCAGGCCAAGAGCTCAAGACCCAACCAGTGTAGTGGTTGCAGTCAGTGAGTGAGTGAGTGACCAGTGAGATAGTGGCATCGATGGCTCGGGGAAGAGGCAGTGCAATGCGAGGCGCCGTCGCCGTCGCGTTTCTCGCCGTCGTCGTGAGCTGCATCTTCCTCTCCGGCTGCGGCGTCGCCGACGCCGCCACCTACTACGTCGGCGACAGCCTCGGCTGGTCCCTCGGCAGCGGGAGCTGGCCCAGCGGCAAGAAGTTCCACGCCGGCGACATCCTCGTGTTCAGGTACTTGCCGTGGATGCACAACGTGGTGGCCGTCGACGAAGACGGGTACGCCGACTGCAACCCGCCGCCGTTCTCGAGGTACTACACCTCCGGCTCCGACAGCGTCAGGCTCGCCAGGGGGGACAACTTCTTCGTCTGCACCCGCTACGGCCACTGCAACCTCGGCATGAAGATGGTCGTCACCGCCGTGTGACTGAGGGAGAAACATGTCAAGACTGTTTCGTACGTCTCGATCGAGCGAAAATGCTGTGAAATGTCACACTCTCTCTATCTTGTTCCATCCGGTTCGTTCTCAGTTTTTAGCATTTTGGATGATTGATTAGAGGATTCTCTAGCTGTTCTATGCCGGTGTGACACGACTCGGTTTGCCAATTGTGTGTGCATTGTTGACGCTGACAAGATGCGTTTGTTCTGAAATCTGAAGAGAAGTCCCTGTCTTTGCCACTGATGATGATATCTACTGCAAATTCTTTAGCGAAGATA
CDS Sequence
  • >LOC_Os03g50140.1
    ATGGCTCGGGGAAGAGGCAGTGCAATGCGAGGCGCCGTCGCCGTCGCGTTTCTCGCCGTCGTCGTGAGCTGCATCTTCCTCTCCGGCTGCGGCGTCGCCGACGCCGCCACCTACTACGTCGGCGACAGCCTCGGCTGGTCCCTCGGCAGCGGGAGCTGGCCCAGCGGCAAGAAGTTCCACGCCGGCGACATCCTCGTGTTCAGGTACTTGCCGTGGATGCACAACGTGGTGGCCGTCGACGAAGACGGGTACGCCGACTGCAACCCGCCGCCGTTCTCGAGGTACTACACCTCCGGCTCCGACAGCGTCAGGCTCGCCAGGGGGGACAACTTCTTCGTCTGCACCCGCTACGGCCACTGCAACCTCGGCATGAAGATGGTCGTCACCGCCGTGTGA
Protein Sequence
  • >LOC_Os03g50140.1
    MARGRGSAMRGAVAVAFLAVVVSCIFLSGCGVADAATYYVGDSLGWSLGSGSWPSGKKFHAGDILVFRYLPWMHNVVAVDEDGYADCNPPPFSRYYTSGSDSVRLARGDNFFVCTRYGHCNLGMKMVVTAV*