Gene Details:

Functional Descriptions:

  • RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1.
  • This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions.
  • The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content.
  • In spite of being a Strategy II plant, however, rice (Oryza sativa) contains the previously identified Fe2+ transporter OsIRT1.
  • Real-time PCR analysis revealed that OsIRT1 and OsIRT2 are expressed predominantly in roots, and these transporters are induced by low-Fe conditions.
  • Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice.
  • Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe and Cu‐deficiency.
  • Yeast mutants expressing OsIRT1 and OsIRT2, which encode the rice Fe2+ transporter, became more sensitive to Cd, suggesting that Cd was absorbed by OsIRT1 and OsIRT2.
  • Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters OsIRT1 and OsIRT2 in rice.
  • A yeast Fe3+ chelate-reductase gene refre1/372, selected for better performance at high pH, was fused to the promoter of the Fe-regulated transporter, OsIRT1, and introduced into rice plants.
  • We generated transgenic rice plants over-expressing OsIRT1 to evaluate its functional roles in metal homeostasis.
  • OsIRT1 promoter-GUS analysis revealed that OsIRT1 is expressed in the epidermis and exodermis of the elongating zone and in the inner layer of the cortex of the mature zone of Fe-deficient roots.
  • Four distinct genes, OsZIP4, OsZIP5, OsZIP6, and OsZIP7 that exhibit sequence similarity to the rice ferrous ion transporter, OsIRT1, were isolated.
  • This demonstrates that OsIRT1 can be used for enhancing micronutrient levels in rice grains.
  • When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron‐uptake mutant.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g46470.1
    AGCTCAAGCAAGCAGCGCGCCTGAGTACGTAGCAAAATCCAGTGTGGTGTACGAACGAAAATGGCGACGCCGCGGACACTGGTGCCCATTCTGCCGCCCGTCGCGGCGCTCCTCCTCCTCCTCGTCGCCGCCTCCTCCATCCCCATCCTCGCCGCCGCGCAGCCGGCGGACGCGTGCGGCGGCGCACCGGATCAGGCGGCGGCGGACGGCGCGTGCCACGACGTGCCGAGGGCGCTGCGGCTGAAGCTGATCGCCATCCCGACCATCCTCGTGTCGAGCGTCGTCGGCGTGTGCCTGCCGCTCCTCTCCCGCTCCGTGCCGGCGCTCCGCCCCGACGGCGGCCTCTTCGCCGTCGTCAAGGCGTTCGCGTCGGGCGTCATCCTCGCCACGGGCTACATGCACGTGCTCCCGGACGCCTTCAACAACCTCACCTCGCCGTGCCTGCCCAGGAAGCCGTGGTCGGAGTTCCCGTTCGCGGCGTTCGTCGCCATGCTCGCCGCCGTGTCCACGCTCATGGCCGACTCGCTCATGCTCACCTACTACAACCGCAGCAAGCCCCGGCCGTCTAGCGGCGGCGACGTCGCCGCCGTCGCCGACCACGGCGAGAGCCCCGACCAGGGCCACCGGCACGGACACGGACACGGCCATGGGCATGGCATGGCGGTGGCCAAGCCCGACGACGTCGAGGCCACTCAGGTGCAGCTGCGCCGGAACCGCGTCGTCGTTCAGGTCCTCGAGATAGGCATCGTGGTGCACTCGGTGGTGATCGGCCTCGGCATGGGGGCGTCGCAGAACGTGTGCACCATCCGGCCGCTGGTGGCGGCGATGTGCTTCCACCAGATGTTCGAGGGGATGGGCCTCGGCGGCTGCATCCTGCAGGCGGAGTACGGCCGCCGGATGAGGTCGGTGCTCGTCTTCTTCTTCTCCACCACGACGCCGTTCGGCATCGCCCTCGGCCTCGCCCTGACCAGGGTGTACAGGGACAACAGCCCGACGGCGCTCATCGTCGTCGGCCTCCTCAACGCCGCCTCCGCGGGGCTGCTCCACTACATGGCGCTGGTGGAGCTCCTCGCCGCCGACTTCATGGGGCCCAAGCTGCAGGGCAACGTCCGCCTCCAGCTCGCCGCCTTCCTCGCCGTCCTCCTCGGCGCCGGCGGCATGTCCGTCATGGCCAAGTGGGCGTGAGCTGACGATGCATCACACTCTGCGGCATCATGCAATTCGCTGCATTGTTAGATTGAGCTTGGTCAGACTCGATCATCAGCTGCAGACATGTTGTACAGTGACTGTGACTTCTCCATGTTGTACTGTAATTCAGGTTTTCAGTGTTGTAGCGTGGTTGTTCAGGTTTTGTTCTTCGTGACTGATGATGTACTTCAATACGAGCATCTACATATTTTCATGATTATTACATTATCAAGCCAAAGAACTATGGTAAGATGGCAAATTTCACACAATATGACAATGTCAACAGGAATGGATGAAAATATGGAATCATGAAAAATTTC
CDS Sequence
  • >LOC_Os03g46470.1
    ATGGCGACGCCGCGGACACTGGTGCCCATTCTGCCGCCCGTCGCGGCGCTCCTCCTCCTCCTCGTCGCCGCCTCCTCCATCCCCATCCTCGCCGCCGCGCAGCCGGCGGACGCGTGCGGCGGCGCACCGGATCAGGCGGCGGCGGACGGCGCGTGCCACGACGTGCCGAGGGCGCTGCGGCTGAAGCTGATCGCCATCCCGACCATCCTCGTGTCGAGCGTCGTCGGCGTGTGCCTGCCGCTCCTCTCCCGCTCCGTGCCGGCGCTCCGCCCCGACGGCGGCCTCTTCGCCGTCGTCAAGGCGTTCGCGTCGGGCGTCATCCTCGCCACGGGCTACATGCACGTGCTCCCGGACGCCTTCAACAACCTCACCTCGCCGTGCCTGCCCAGGAAGCCGTGGTCGGAGTTCCCGTTCGCGGCGTTCGTCGCCATGCTCGCCGCCGTGTCCACGCTCATGGCCGACTCGCTCATGCTCACCTACTACAACCGCAGCAAGCCCCGGCCGTCTAGCGGCGGCGACGTCGCCGCCGTCGCCGACCACGGCGAGAGCCCCGACCAGGGCCACCGGCACGGACACGGACACGGCCATGGGCATGGCATGGCGGTGGCCAAGCCCGACGACGTCGAGGCCACTCAGGTGCAGCTGCGCCGGAACCGCGTCGTCGTTCAGGTCCTCGAGATAGGCATCGTGGTGCACTCGGTGGTGATCGGCCTCGGCATGGGGGCGTCGCAGAACGTGTGCACCATCCGGCCGCTGGTGGCGGCGATGTGCTTCCACCAGATGTTCGAGGGGATGGGCCTCGGCGGCTGCATCCTGCAGGCGGAGTACGGCCGCCGGATGAGGTCGGTGCTCGTCTTCTTCTTCTCCACCACGACGCCGTTCGGCATCGCCCTCGGCCTCGCCCTGACCAGGGTGTACAGGGACAACAGCCCGACGGCGCTCATCGTCGTCGGCCTCCTCAACGCCGCCTCCGCGGGGCTGCTCCACTACATGGCGCTGGTGGAGCTCCTCGCCGCCGACTTCATGGGGCCCAAGCTGCAGGGCAACGTCCGCCTCCAGCTCGCCGCCTTCCTCGCCGTCCTCCTCGGCGCCGGCGGCATGTCCGTCATGGCCAAGTGGGCGTGA
Protein Sequence
  • >LOC_Os03g46470.1
    MATPRTLVPILPPVAALLLLLVAASSIPILAAAQPADACGGAPDQAAADGACHDVPRALRLKLIAIPTILVSSVVGVCLPLLSRSVPALRPDGGLFAVVKAFASGVILATGYMHVLPDAFNNLTSPCLPRKPWSEFPFAAFVAMLAAVSTLMADSLMLTYYNRSKPRPSSGGDVAAVADHGESPDQGHRHGHGHGHGHGMAVAKPDDVEATQVQLRRNRVVVQVLEIGIVVHSVVIGLGMGASQNVCTIRPLVAAMCFHQMFEGMGLGGCILQAEYGRRMRSVLVFFFSTTTPFGIALGLALTRVYRDNSPTALIVVGLLNAASAGLLHYMALVELLAADFMGPKLQGNVRLQLAAFLAVLLGAGGMSVMAKWA*