Gene Details:

Functional Descriptions:

  • The OsPUB24 T-DNA knock-out mutant and Ubi:RNAi-OsPUB24 knock-down rice plants displayed enhanced seedling growth, increased lamina joint bending, and hypersensitivity to brassinolide (BL).
  • OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in rice BR response.
  • Here, we report that a rice U-box E3 ubiquitin (Ub) ligase OsPUB24 acts as a negative regulator in the BR signaling pathway via 26S proteasome-dependent degradation of OsBZR1.
  • Our findings suggest that OsPUB24 participates in OsBZR1 turnover and the regulatory networks of OsPUB24, OsSK22, and OsBZR1 are crucial for fine-tuning the BR response in rice.
  • In addition, the stability of OsPUB24 was downregulated by BL and bikinin, an inhibitor of OsSK22 (Oryza sativa Shaggy/GSK3-like kinase 22).
  • OsSK22, the homolog of Arabidopsis BIN2 (BRASSINOSTEROID INSENSITIVE2) protein kinase, phosphorylated OsPUB24 and elevated the cellular stability of OsPUB24.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g45420.1
    GGGTTTGTTCTGGCTCGCACACAGCTCAGCAGCTAACTAGTCCAATGGAGCAGCTAGCAGGTGAGAAAATAAAAGCCAAGAACAAGTCGACGTGGCCGCCTTTCGTTACCCACCAACCCAACCTCGTTCTACGGTACGCCACGCCTCCCCTCCCCTCCTCCGCCGCCGCCGCCTCAATTAGACCGAAGCTCTAAACTCCGGCGAGCCCTCGCCGCCGCCTGCGTGGAGGGGAGGTCGGGAGGAGGGGGTTGATGGCGGGCGAGGGCGTGGAGATGTCGGAGGAGGAAGGCGCGTTCGAGGCGTTCGTGTGCCCGCTGACGAAGCAGGTGATGCGCGACCCGGTGACGATCGAGACCGGGCAGACGTTCGAGCGGGAGGCGATCCTCAAGTGGTTCCGCGAGTGCCGGGACAACGGCCGCCGCCCGACGTGCCCGCTCACGCAGCGCGAGCTCCGCGACACGGAGGTGTCGCCCAGCGTGGCGCTCCGCAGCGTCATCCACGAGTGGCGGGCGCGCAACGAGGAGAAGGACCTCGACCGCGCCTGCGCCTCCCTCGTCGGCGGCTTCGCCGGCCACGCCGGCGACGAGGAGGAGGAGGAGTCCGCGCTCCGTGCCCTGGTGCACGTCTCCCAGATATGCCAGCGGAGCGCCGCCAGCAAGGACCTCGTCCGCCGCCGCGGCGTCCTCCGCGCCGTCGCCGAGATGCTCAAGAGCGGCAGCCGACGGCTCCGCCTCAAGTCCCTCCAGGTCCTCCGCGTCCTCGTCGAAGACAACGACGATAACAAGGAGGAGTTGGGGAAGGGCGACACGATACGCACCATCATCAAGTTCTTGTCGAACGAGCATGTCCAGGAGCGGGAGCTGGCGGTGTCGCTGCTGCACGAGCTGTCCGGGCACGAGCCGACGTGCGAGCGGATCGGCGCGGTGTACGGCGCCATCCTGCTGCTGGTCGGGATGGGGAGCAGCAAGTCGGAGAGCGCCGTCGCCGTCGACAAGGCGGAGAGCACGCTCCGCAACCTCGACCGCTTCGACGCCAACGTCAAGCAGATGGCCGACAACGGCAGGCTGCAGCCGCTCCTCACCAGGCTGCTCCGCGGCGAGCCCGACACGCGGGTCGCCATGGCCGACTACCTCGGCGAGCTCGCCCTGGCGAACGACGACAAGGCCGCGGTGGCCGAGCAGGCCGGCCCGCTGCTGGTGGGGATGCTCCGGACCGGCGCCACGCCGGCGAAGGAGGCCACGCTCAAGGCGCTCAGGGAGATCTCATCCAGCGAGGCGAGCGCCAAGCTGCTGCTGCAGCGCGCCGGCGTCCTGCCCCCGCTCGTCAACGATGTCCTCTTCTCCACGGGACACCTACCGATGAAGCTCAAGGAGCTCGCCGCCACCATCCTCGCCAACCTCGTCGCCTCAGGCGCCGATTTCCGTTCCATCCCACTCGACGACGACGAAGACGACGACGGCGGCGGCGGCGGACGTGGGCGCCGCAGGACGCTCCTGTCGGAGGACGTCGTGCATAGCCAGCTCCACCTCATCAGCAACACCGGCCCCGCCATCGGATGCAGACTCCTCAGCGTCCTCGCCGGGTTGACCAGCTCCCGCGCGACGGTGGCCGACGTCGTCGCGGCGGTCAAGAGCTCCGGCGCCACAATCAGCCTCATCCAGTTCATCGAGGCAGCGCACCGGGACATCCGCGTCGAGTCCCTCAAGCTCCTGCGCAACCTCGCCCCCTACATGGGCGCCGAGCTCGCCGACGCCCTGGGCGGAAGCCTGAGCAGCCTCCTCCGGGCCATCTCCTCCGACGGCGGCGGCGTCACGGAGGAGCAGGCGGCTGCCGTGGGGTTGCTCGGCGACCTCCCGGAGGGTGACTCCAGCCTGACCAGGCAGCTGTTCGACCTGGGCGCGTTCAGGGCGCTGGCCCCGAAGCTGGCGGAGCTCCGGCGGGGCACCATCCGCGGCGGGAACCGGTACGTGACGCCGCTGACGGAGGGCGTGGTGAAGGTGATGTACCGGGTGACGTGCGCGCTGGAGGAGGACGCGGAGTACGTGGAGTTCGCGCGGGAGGCCGGCCTGGCGCCGCTGTTCGTCGAGCTGCTCCACACCAACGGCATGGACACGGTGCAGCTCTACTCGGCAATGGCGCTGGAGAAGCTGTCGCTGCAGTCGAGCCACCTGACGGCCATCCCGGCGCCGCCGTCGCCACCGGCGGGGTTCGGGTGCGCGTGCTTGGGGAGGAGACCCGCGGCGGCGGCGGTGCCCGCGGGGGTGTGCCGCGTGCACGGCGGGTTCTGCTCGCTGCGGGAGACGTTCTGCCTGGCGCAGGCGGACGGCGGGAAGGCGGTGGAGCGGCTGGTGGCGTGCCTGGACCACCTGGACGGGAGGGTGGTGGAGGCGGCGCTGGCGGCGCTGTCGACGCTGGTGTGCGACGGCGTGGACGCGAGGGAAGGGGTGGTGGTGCTGGGGGAAGCCGACGGGCTGCGCCCGGTGGTGGACATCATGGTGGAGAGCCGCACGGAGGCGCTGCAGCGGCGGGCGGTGTGGGCGGTGGAGCGCATCCTGCGGGTGGAGGAGATCGCCGGGGAGGTGGCCGCCGACCAGACGGTGGCGTCGGCGCTCGTCGAGGCGTACCGCAACGGCGACCCGCGCACCCGGCAGACGGCGGAGCGCGCGCTCCGCCACCTCGACCGGATCCCCAACTTCTCCGCCGCCTTCCAATCCAAACGCTCATAGCCTCTCCACAGATCTGCCATTGCCAATGTGCAACTGCAACTCTGCAAGGCTCTCCCTTCGCTGATTATCCCATTCCCCTCTTTCATTTCACTTGCTCTTTTTCCCATTCCCCTCTCACTTCACTTGCTCTTTTGCTGGATCGGATTGCTGATTTTTTTTTCTTTTTTTTGGTTTGTTTACAGCAGCATGATGCTTGTATAGCATAATCGTCTTAATTCTTTCAAAGCAAAATGGATGAAACAAAATTGGTTGCACCTCGAGTGCATTTTTCTGTGAGAATTAATCGAGACTGGGTTTGTAAATACACTAATGTTTCCAAGAGCAATCACAACAATCTCAGTCAAAGAGCTCCACCATCCAAACAGTCCATGACATTCTACTGTAAAACGCAACGCATCCACAGTACATATGTTCTGTTTGTTAAAAGTTAGCCGCCCATTTCTGTAATAAAATGTTTCGCTACAGATCTATTTGTGAAGGGAAAAAAATCAGCACGGGCTAAAGTACGATAACTCCACGTCTCCACCTCGTTCGACTGAACGCGATACTCAAGTCTCTCAGTCACCCAAGCTACAAGCCTACTACAACAAGTCTACAACACTCGTGCATCGAGGGGCTCACATAAATTACAAGATGCTATACGCTCCTCCAGACCATATGTACAGTTAAGATTAGCAGTCACTAGGCAGCAAAATCTCAAGGAAGCTTGTACTTGTGTATTCCATAAGTTTTC
CDS Sequence
  • >LOC_Os03g45420.1
    ATGGCGGGCGAGGGCGTGGAGATGTCGGAGGAGGAAGGCGCGTTCGAGGCGTTCGTGTGCCCGCTGACGAAGCAGGTGATGCGCGACCCGGTGACGATCGAGACCGGGCAGACGTTCGAGCGGGAGGCGATCCTCAAGTGGTTCCGCGAGTGCCGGGACAACGGCCGCCGCCCGACGTGCCCGCTCACGCAGCGCGAGCTCCGCGACACGGAGGTGTCGCCCAGCGTGGCGCTCCGCAGCGTCATCCACGAGTGGCGGGCGCGCAACGAGGAGAAGGACCTCGACCGCGCCTGCGCCTCCCTCGTCGGCGGCTTCGCCGGCCACGCCGGCGACGAGGAGGAGGAGGAGTCCGCGCTCCGTGCCCTGGTGCACGTCTCCCAGATATGCCAGCGGAGCGCCGCCAGCAAGGACCTCGTCCGCCGCCGCGGCGTCCTCCGCGCCGTCGCCGAGATGCTCAAGAGCGGCAGCCGACGGCTCCGCCTCAAGTCCCTCCAGGTCCTCCGCGTCCTCGTCGAAGACAACGACGATAACAAGGAGGAGTTGGGGAAGGGCGACACGATACGCACCATCATCAAGTTCTTGTCGAACGAGCATGTCCAGGAGCGGGAGCTGGCGGTGTCGCTGCTGCACGAGCTGTCCGGGCACGAGCCGACGTGCGAGCGGATCGGCGCGGTGTACGGCGCCATCCTGCTGCTGGTCGGGATGGGGAGCAGCAAGTCGGAGAGCGCCGTCGCCGTCGACAAGGCGGAGAGCACGCTCCGCAACCTCGACCGCTTCGACGCCAACGTCAAGCAGATGGCCGACAACGGCAGGCTGCAGCCGCTCCTCACCAGGCTGCTCCGCGGCGAGCCCGACACGCGGGTCGCCATGGCCGACTACCTCGGCGAGCTCGCCCTGGCGAACGACGACAAGGCCGCGGTGGCCGAGCAGGCCGGCCCGCTGCTGGTGGGGATGCTCCGGACCGGCGCCACGCCGGCGAAGGAGGCCACGCTCAAGGCGCTCAGGGAGATCTCATCCAGCGAGGCGAGCGCCAAGCTGCTGCTGCAGCGCGCCGGCGTCCTGCCCCCGCTCGTCAACGATGTCCTCTTCTCCACGGGACACCTACCGATGAAGCTCAAGGAGCTCGCCGCCACCATCCTCGCCAACCTCGTCGCCTCAGGCGCCGATTTCCGTTCCATCCCACTCGACGACGACGAAGACGACGACGGCGGCGGCGGCGGACGTGGGCGCCGCAGGACGCTCCTGTCGGAGGACGTCGTGCATAGCCAGCTCCACCTCATCAGCAACACCGGCCCCGCCATCGGATGCAGACTCCTCAGCGTCCTCGCCGGGTTGACCAGCTCCCGCGCGACGGTGGCCGACGTCGTCGCGGCGGTCAAGAGCTCCGGCGCCACAATCAGCCTCATCCAGTTCATCGAGGCAGCGCACCGGGACATCCGCGTCGAGTCCCTCAAGCTCCTGCGCAACCTCGCCCCCTACATGGGCGCCGAGCTCGCCGACGCCCTGGGCGGAAGCCTGAGCAGCCTCCTCCGGGCCATCTCCTCCGACGGCGGCGGCGTCACGGAGGAGCAGGCGGCTGCCGTGGGGTTGCTCGGCGACCTCCCGGAGGGTGACTCCAGCCTGACCAGGCAGCTGTTCGACCTGGGCGCGTTCAGGGCGCTGGCCCCGAAGCTGGCGGAGCTCCGGCGGGGCACCATCCGCGGCGGGAACCGGTACGTGACGCCGCTGACGGAGGGCGTGGTGAAGGTGATGTACCGGGTGACGTGCGCGCTGGAGGAGGACGCGGAGTACGTGGAGTTCGCGCGGGAGGCCGGCCTGGCGCCGCTGTTCGTCGAGCTGCTCCACACCAACGGCATGGACACGGTGCAGCTCTACTCGGCAATGGCGCTGGAGAAGCTGTCGCTGCAGTCGAGCCACCTGACGGCCATCCCGGCGCCGCCGTCGCCACCGGCGGGGTTCGGGTGCGCGTGCTTGGGGAGGAGACCCGCGGCGGCGGCGGTGCCCGCGGGGGTGTGCCGCGTGCACGGCGGGTTCTGCTCGCTGCGGGAGACGTTCTGCCTGGCGCAGGCGGACGGCGGGAAGGCGGTGGAGCGGCTGGTGGCGTGCCTGGACCACCTGGACGGGAGGGTGGTGGAGGCGGCGCTGGCGGCGCTGTCGACGCTGGTGTGCGACGGCGTGGACGCGAGGGAAGGGGTGGTGGTGCTGGGGGAAGCCGACGGGCTGCGCCCGGTGGTGGACATCATGGTGGAGAGCCGCACGGAGGCGCTGCAGCGGCGGGCGGTGTGGGCGGTGGAGCGCATCCTGCGGGTGGAGGAGATCGCCGGGGAGGTGGCCGCCGACCAGACGGTGGCGTCGGCGCTCGTCGAGGCGTACCGCAACGGCGACCCGCGCACCCGGCAGACGGCGGAGCGCGCGCTCCGCCACCTCGACCGGATCCCCAACTTCTCCGCCGCCTTCCAATCCAAACGCTCATAG
Protein Sequence
  • >LOC_Os03g45420.1
    MAGEGVEMSEEEGAFEAFVCPLTKQVMRDPVTIETGQTFEREAILKWFRECRDNGRRPTCPLTQRELRDTEVSPSVALRSVIHEWRARNEEKDLDRACASLVGGFAGHAGDEEEEESALRALVHVSQICQRSAASKDLVRRRGVLRAVAEMLKSGSRRLRLKSLQVLRVLVEDNDDNKEELGKGDTIRTIIKFLSNEHVQERELAVSLLHELSGHEPTCERIGAVYGAILLLVGMGSSKSESAVAVDKAESTLRNLDRFDANVKQMADNGRLQPLLTRLLRGEPDTRVAMADYLGELALANDDKAAVAEQAGPLLVGMLRTGATPAKEATLKALREISSSEASAKLLLQRAGVLPPLVNDVLFSTGHLPMKLKELAATILANLVASGADFRSIPLDDDEDDDGGGGGRGRRRTLLSEDVVHSQLHLISNTGPAIGCRLLSVLAGLTSSRATVADVVAAVKSSGATISLIQFIEAAHRDIRVESLKLLRNLAPYMGAELADALGGSLSSLLRAISSDGGGVTEEQAAAVGLLGDLPEGDSSLTRQLFDLGAFRALAPKLAELRRGTIRGGNRYVTPLTEGVVKVMYRVTCALEEDAEYVEFAREAGLAPLFVELLHTNGMDTVQLYSAMALEKLSLQSSHLTAIPAPPSPPAGFGCACLGRRPAAAAVPAGVCRVHGGFCSLRETFCLAQADGGKAVERLVACLDHLDGRVVEAALAALSTLVCDGVDAREGVVVLGEADGLRPVVDIMVESRTEALQRRAVWAVERILRVEEIAGEVAADQTVASALVEAYRNGDPRTRQTAERALRHLDRIPNFSAAFQSKRS*