Information report for OsNCED3
Gene Details
|
|
Functional Descriptions
- The data suggest that, in addition to ABA biosynthesis, the monocot OsNCED3 gene may have additional functions in shaping leaf morphology and vascular bundle development in Arabidopsis.
- Ectopic expression of rice OsNCED3 in Arabidopsis increases ABA level and alters leaf morphology.
- Furthermore, overexpression of OsNCED3 in wild-type Arabidopsis plants results in increased accumulation of ABA, reduced relative water loss, delayed seed germination, and greater drought tolerance relative to that of wild-type.
- As a key gene in ABA biosynthesis, OsNCED3 was significantly induced in rice by water stress treatment and such induction preceded the rapid increase in ABA.
- The differences in endogenous ABA levels in Doongara and R31 correlated with differences in expression of the ABA biosynthetic genes encoding zeaxanthin epoxidase (OSZEP1) and 9-cis-epoxycarotenoid dioxygenase (OSNCED2, OsNCED3) in anthers.
- Overexpression of OsFbx352 led to a reduction in expression of genes responsible for ABA synthesis (OsNced2, OsNCED3) and an increase in expression of genes encoding ABA catabolism (OsAba-ox2, OsAba-ox3) in the presence of glucose.
- Transgenic Arabidopsis lines overexpressing the monocot OsNCED3 gene in a wild-type background result in a smaller and rounder leaf shape and midvein.
- Further analysis found that nced3 mutants contained lower ABA content compared with wild-type plants, overexpression of OsNCED3 in transgenic plants could enhance water stress tolerance, promote leaf senescence and increase ABA content.
- We conclude that OsNCED3 mediates seed dormancy, plant growth, abiotic stress tolerance, and leaf senescence by regulating ABA biosynthesis in rice; and may provide a new strategy for improving the quality of crop.
- Here, we report important functions of OsNCED3 in multi-abiotic stress tolerance in rice.
- The OsNCED3 is constitutively expressed in various tissues under normal condition, Its expression is highly induced by NaCl, PEG, and H2O2 stress, suggesting the roles for OsNCED3 in response to the multi-abiotic stress tolerance in rice.
- CRISPR/Cas9-edited OsNCED3 resulted in chilling sensitive of japonica rice ZH11, demonstrating the involvement of ABA pathway in chilling stress response.
- SiMYB19 overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene OsNCED3 and the ABA signal transduction pathway-related genes OsPK1 and OsABF2.
- This study shows that overexpression of OsNCED3 in rice improves resistance to BPH, and has potential merit in rice breeding programs.
- Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens.
- In rice, OsNCED3 was shown to promote ABA synthesis, and improve abiotic stress tolerance, but the function of OsNCED3 in regulating rice defense against the brown planthopper (Nilaparvata lugens; BPH) has been unclear.
- Rice lines overexpressing OsNCED3 (OE) were more resistant to BPH than the wild-type cv.
- Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2.
- Disruption of OsNCED3 by CRISPR/Cas9-mediated mutagenesis led to a lower ABA and higher gibberellic acid (GA) levels (thus a skewed ABA/GA ratio) in the embryo, promoting embryos growth and breaking seed dormancy before seed maturity and harvest, thus decreased seed dormancy and enhanced PHS in rice.
- ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice.
- Intriguingly, the overexpression of OsNCED3 resulted in increased grain size and weight, whereas the disruption of OsNCED3 function decreased grain size and weight.
- However, the overexpression of OsNCED3 enhanced PHS resistance by regulating proper ABA/GA ratio in the embryo.
- Nucleotide diversity analyses suggested that OsNCED3 may be selected during japonica populations adaptation of seed dormancy and germination.
- OsNCED3 encodes a chloroplast-localized abscisic acid (ABA) biosynthetic enzyme highly expressed in the embryo of developing seeds.
Functional Keywords
- ABA , seed , anther , drought-tolerance , vascular-bundle , leaf , leaf-shape , seed-germination , drought , leaf-senescence , senescence , tolerance , abiotic-stress , stress , biotic-stress , quality , stress-tolerance , plant-growth , ABA-biosynthesis , chilling , stress-response , chilling-stress , abscisic-acid , signal-transduction , resistance , defense , brown-planthopper , breeding , resistant , growth , development , grain , grain-size , dormancy , embryo , seed-dormancy
Literature and News
- Ectopic expression of rice OsNCED3 in Arabidopsis increases ABA level and alters leaf morphology . DOI: 10.1016/j.plantsci.2009.09.014
- Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L . DOI: 10.1007/s00425-013-1853-9 ; PMID: 23397192
- ABA controls HO accumulation through the induction of OsCATB in rice leaves under water stress . DOI: 10.1093/pcp/pcr028 ; PMID: 21398647
- The rice ERF transcription factor OsERF922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance . DOI: 10.1093/jxb/ers079 ; PMID: 22442415
- A rice F-box gene, OsFbx352, is involved in glucose-delayed seed germination in rice . DOI: 10.1093/jxb/ers206 ; PMID: 22859682
- ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice . DOI: 10.1093/pcp/pcm100 ; PMID: 17693452
- Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8’-hydroxylase in rice . DOI: 10.1093/pcp/pcm003 ; PMID: 17205969
- Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis . DOI: 10.1093/pcp/pcp022 ; PMID: 19208695
- 9- cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice . DOI: 10.3389/fpls.2018.00162 ; PMID: 29559982
- The Wild Rice Locus CTS-12 Mediates ABA-Dependent Stomatal Opening Modulation to Limit Water Loss Under Severe Chilling Stress . DOI: 10.3389/fpls.2020.575699 ; PMID: 33193516
- SiMYB19 from Foxtail Millet (Setaria italica) Confers Transgenic Rice Tolerance to High Salt Stress in the Field . DOI: 10.3390/ijms23020756 ; PMID: 35054940
- Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens . DOI: 10.1186/s12864-022-08846-5 ; PMID: 36064309
- OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage . DOI: 10.3390/ijms231810656 ; PMID: 36142568
- ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice . DOI: 10.1111/pce.14480 ; PMID: 36319615
Gene Resources
- UniProt: Q5MBR5
- EMBL: AP008209, AP014959, AY838899
- AlphaFoldDB: Q5MBR5
- EnsemblPlants: Os03t0645900-01
- Gramene: Os03t0645900-01
- KEGG: dosa:Os03g0645900
Sequences
cDNA Sequence
- >LOC_Os03g44380.1
CCACCCCCCCCCCCCCCCCCCTCCAACTTGCCAATGCGCCTCTCCCTACCTCCTCCTCCGTCTCCCCTGCTCTCCTCTGCTCCGTTAACTCTATATATACGCTCACTGGCTACCTCCCCTCCCAAACCATCCAAACCGACGAGCTCTCTTCCTCTCTCTCCAGCTCGGTCTCACACACACTCTCTCTCTCTCGGCAGAAACACACCACGATCGCGGCCAAAACCCACGCGATTGTAGCTCAGATTGTTGTGCGATCGACGCGATATGGCGACGATCACGACGCCAGGATATGCTCACATACAGCGGCAGCACGGCAGGTGCTCGACGACGGCGGGAAGGCGTGGGGCGTCCAATTCGGTGAGATTCTCCGCGCGCGCGGTTAGCTCCGTGCCGCACGCGGCGGCGGCGTCATCGGCGCCGGCGTTCCTGCCGGTGCCGTTCGTGCCTGGGGCCGACGCACCGTCGCCGTCGGGGAAGAGTGCCATTGGCGTCCCGAAGGCGCCGAGGAAGGGGGAGGAGGGGAAGAGGCTCAACTTCTTCCAGCGCGCCGCGGCGATGGCGCTCGACGCGTTCGAGGAGGGGTTTGTGGCGAATGTCCTCGAGCGCCCGCACGGGCTGCCGAGCACGGCCGACCCCGCGGTGCAGATCGCCGGCAACTTCGCGCCGGTCGGTGAGACGCCGCCCGCGCGCGCGCTGCCGGTGTCGGGGCGCATCCCGCCCTTCATCAACGGCGTCTACGCGCGCAACGGCGCCAACCCGCACTTCGACCCCGTCGCCGGGCACCACCTGTTCGACGGCGATGGCATGGTGCACGCCGTCAGGATACGCAACGGCGCCGCCGAGTCGTACGCGTGCCGGTTCACGGAGACCGCGCGGCTGCGGCAGGAGCGCGCGATGGGGCGGCCCATGTTCCCCAAGGCCATTGGGGAGCTCCATGGCCACTCCGGCATCGCGCGCCTTGCTCTGTTCTACGCGCGCGCCGCCTGCGGCCTCCTCGACCCGTCACACGGCACCGGCGTCGCCAACGCCGGCCTCATCTACTTCAACGGCAGGCTCCTCGCCATGTCGGAGGACGACCTCCCCTACCAGGTGCGCGTCACCGCCGACGGCGACCTCGAGACCGTCGGCCGCTACGACTTCGACGGGCAGCTCGGCTGCGCCATGATCGCGCACCCCAAGCTCGACCCGGCCACCGGAGAGCTCCACGCGCTCAGCTACGACGTGATCAAGAAGCCGTACCTCAAGTACTTCTACTTCGCGCCCGACGGCACCAAGTCGGCCGACGTCGAGATCCCGCTCGACCAGCCCACCATGATCCACGACTTCGCCATCACCGAGAACTACGTGGTGGTACCCGACCACCAGGTGGTGTTCAAGCTCCAGGAGATGCTCCGCGGCGGCTCGCCCGTGGTGCTCGACAAGGAGAAGACGTCGCGGTTCGGGGTGCTCCCCAAGCACGCCGCGGACGCGTCGGAGATGGTGTGGGTGGACGTCCCGGACTGCTTCTGCTTCCACCTCTGGAACGCGTGGGAGGAGGCGGACACCGACGAGGTGGTGGTGATCGGCTCGTGCATGACCCCCGCCGACTCCATCTTCAACGAGTCCGACGACCGCCTCGAGAGCGTCCTCACCGAGATCCGCCTCAACACCCGCACCGGCGAGTCGACGCGGCGCGCCATCCTGCCGCCGTCGAGCCAGGTCAACCTCGAGGTGGGCATGGTCAACCGCAACCTCCTCGGCCGCAAGACGCGGTACGCCTACCTCGCCGTGGCCGAGCCGTGGCCCAAGGTGTCGGGCTTCGCCAAGGTGGACCTCGCCACGGGTGAGCTCACCAAGTTCGAGTACGGCGAGGGCCGGTTCGGCGGCGAGCCCTGCTTCGTCCCCATGGACGCCGCCGCCGCCACGCCCCGCGGCGAGGACGACGGCTACATCCTGTCCTTCGTCCACGACGAGCGCGCCGGGACCTCCGAGCTCCTCGTCGTCAATGCCGCCGACATGCGCCTTGAGGCCACCGTGCAGCTGCCGTCCCGCGTGCCGTACGGCTTCCACGGCACGTTCATCACCGGCGACGAGCTCACCACCCAGGCCTGATCCATCGATCGAACACCTCCACGTTTCTTGGGGGGGGGAGGAAGTGACCAGAGGGAGCCTGACCGATAGGTCCCCGGATTCCACCCCCCCCCCCCCCCTTCCCTTCCAGATACAGTTACAGTTACAGTTACAGTTAGTATAGTAGTTAGCCTCGGTCTTCCAATTTTTAGCTCACTCGCGTCAGCTGAGTCCACACTAGTCCAATAGAGAGAGAGATTATTAGTCCAAGATAGAGAGAGAGAGGGCCCAGCTCGTAGCTTTTGTGTTGGGTGCTCGCTTTGCTTCCAGTGAGCAACCAAGAGGTCCAGAGCTCAGCCGGTGCCTATCTACCAGATTACTAGTAGTATATATGTTTTTTTTTTCATCTTTTTCTTCCCTTTTTGTTTGGATTAGACAGGGATATGCCCCCTGCCCCGGGTTGTCGTCGTCACCACTGTGCAGAGTTTGGGATTCTTGCTCCCCACCCAGTTGCTGCTCCTGCTGCTGAGGTGTTGTTTTGGCTTATGCCACCCTCCCGAGTGTGTACATTTGTTCATAGCTTGTATAATAAAATCTGCCTCTCCCATTGGTTTCCATGTCA
CDS Sequence
- >LOC_Os03g44380.1
ATGGCGACGATCACGACGCCAGGATATGCTCACATACAGCGGCAGCACGGCAGGTGCTCGACGACGGCGGGAAGGCGTGGGGCGTCCAATTCGGTGAGATTCTCCGCGCGCGCGGTTAGCTCCGTGCCGCACGCGGCGGCGGCGTCATCGGCGCCGGCGTTCCTGCCGGTGCCGTTCGTGCCTGGGGCCGACGCACCGTCGCCGTCGGGGAAGAGTGCCATTGGCGTCCCGAAGGCGCCGAGGAAGGGGGAGGAGGGGAAGAGGCTCAACTTCTTCCAGCGCGCCGCGGCGATGGCGCTCGACGCGTTCGAGGAGGGGTTTGTGGCGAATGTCCTCGAGCGCCCGCACGGGCTGCCGAGCACGGCCGACCCCGCGGTGCAGATCGCCGGCAACTTCGCGCCGGTCGGTGAGACGCCGCCCGCGCGCGCGCTGCCGGTGTCGGGGCGCATCCCGCCCTTCATCAACGGCGTCTACGCGCGCAACGGCGCCAACCCGCACTTCGACCCCGTCGCCGGGCACCACCTGTTCGACGGCGATGGCATGGTGCACGCCGTCAGGATACGCAACGGCGCCGCCGAGTCGTACGCGTGCCGGTTCACGGAGACCGCGCGGCTGCGGCAGGAGCGCGCGATGGGGCGGCCCATGTTCCCCAAGGCCATTGGGGAGCTCCATGGCCACTCCGGCATCGCGCGCCTTGCTCTGTTCTACGCGCGCGCCGCCTGCGGCCTCCTCGACCCGTCACACGGCACCGGCGTCGCCAACGCCGGCCTCATCTACTTCAACGGCAGGCTCCTCGCCATGTCGGAGGACGACCTCCCCTACCAGGTGCGCGTCACCGCCGACGGCGACCTCGAGACCGTCGGCCGCTACGACTTCGACGGGCAGCTCGGCTGCGCCATGATCGCGCACCCCAAGCTCGACCCGGCCACCGGAGAGCTCCACGCGCTCAGCTACGACGTGATCAAGAAGCCGTACCTCAAGTACTTCTACTTCGCGCCCGACGGCACCAAGTCGGCCGACGTCGAGATCCCGCTCGACCAGCCCACCATGATCCACGACTTCGCCATCACCGAGAACTACGTGGTGGTACCCGACCACCAGGTGGTGTTCAAGCTCCAGGAGATGCTCCGCGGCGGCTCGCCCGTGGTGCTCGACAAGGAGAAGACGTCGCGGTTCGGGGTGCTCCCCAAGCACGCCGCGGACGCGTCGGAGATGGTGTGGGTGGACGTCCCGGACTGCTTCTGCTTCCACCTCTGGAACGCGTGGGAGGAGGCGGACACCGACGAGGTGGTGGTGATCGGCTCGTGCATGACCCCCGCCGACTCCATCTTCAACGAGTCCGACGACCGCCTCGAGAGCGTCCTCACCGAGATCCGCCTCAACACCCGCACCGGCGAGTCGACGCGGCGCGCCATCCTGCCGCCGTCGAGCCAGGTCAACCTCGAGGTGGGCATGGTCAACCGCAACCTCCTCGGCCGCAAGACGCGGTACGCCTACCTCGCCGTGGCCGAGCCGTGGCCCAAGGTGTCGGGCTTCGCCAAGGTGGACCTCGCCACGGGTGAGCTCACCAAGTTCGAGTACGGCGAGGGCCGGTTCGGCGGCGAGCCCTGCTTCGTCCCCATGGACGCCGCCGCCGCCACGCCCCGCGGCGAGGACGACGGCTACATCCTGTCCTTCGTCCACGACGAGCGCGCCGGGACCTCCGAGCTCCTCGTCGTCAATGCCGCCGACATGCGCCTTGAGGCCACCGTGCAGCTGCCGTCCCGCGTGCCGTACGGCTTCCACGGCACGTTCATCACCGGCGACGAGCTCACCACCCAGGCCTGA
Protein Sequence
- >LOC_Os03g44380.1
MATITTPGYAHIQRQHGRCSTTAGRRGASNSVRFSARAVSSVPHAAAASSAPAFLPVPFVPGADAPSPSGKSAIGVPKAPRKGEEGKRLNFFQRAAAMALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGETPPARALPVSGRIPPFINGVYARNGANPHFDPVAGHHLFDGDGMVHAVRIRNGAAESYACRFTETARLRQERAMGRPMFPKAIGELHGHSGIARLALFYARAACGLLDPSHGTGVANAGLIYFNGRLLAMSEDDLPYQVRVTADGDLETVGRYDFDGQLGCAMIAHPKLDPATGELHALSYDVIKKPYLKYFYFAPDGTKSADVEIPLDQPTMIHDFAITENYVVVPDHQVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKHAADASEMVWVDVPDCFCFHLWNAWEEADTDEVVVIGSCMTPADSIFNESDDRLESVLTEIRLNTRTGESTRRAILPPSSQVNLEVGMVNRNLLGRKTRYAYLAVAEPWPKVSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMDAAAATPRGEDDGYILSFVHDERAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFITGDELTTQA*