Gene Details:

Functional Descriptions:

  • Map-based cloning revealed that HSA1 encodes a putative OsFLN2.
  • The HSA1 mutant harbors a recessive mutation in a gene encoding fructokinase-like protein2 (FLN2); the mutation causes a premature stop codon and results in a severe albino phenotype, with defects in early chloroplast development.
  • The color of HSA1 mutant plants gradually changed from albino to green at later stages of development at various temperatures and chloroplast biogenesis was strongly delayed at high temperature (32<U+202F>C).
  • HSA1 localizes to the chloroplast and regulates chloroplast development.
  • These results demonstrated that HSA1 plays important roles in chloroplast development at early stages, and functions in protecting chloroplasts under heat stress at later stages in rice.
  • GRA117 encodes PfkB-type fructokinase-like 2, which is subcellularly localized in chloroplasts and is widely expressed in various rice tissues, particularly at high levels in leaf tissues.
  • RNA-Seq analysis revealed that GRA117 plays a significant role in photosynthetic carbon fixation, carbon metabolism, and chloroplast ribosome-related pathways.
  • Our study supports that GRA117 promotes the Calvin-Benson cycle by regulating chloroplast development, ultimately leading to enhanced carbon assimilation in rice.
  • GRA117 is crucial in the process of carbon assimilation in rice as it regulates the development of chloroplasts, which in turn facilitates the Calvin-Benson cycle.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g40550.1
    CTGCTCAGCTCTGAGCTTCTTCGTTTGAGGGATTTTCTTCTCCCGGGAGGCGGAAGAGGCGTAGGCGAGGATTCTTGTAGGAATCCTCTCCTCTCCATTGATGCACCGAATGGCTTCTCTTCTTCTCCCCCCGCAGTTTCTTTGCTCCCTGCCTTGTAGTACCAACTCAATCAGGAGCCATTTACATTATAAGCCCCACTTCTTGGGAAACATTATGACTAAGCCTAAAGCTAAAATGAGGCTGCTCAACCGAAACGTAAGTTCCATGGCAAAGAAGAGCTCTCAAGATGTAGCAGAAGGTTCAAGTGATGATGAGAGTGACGGCGAGACATCAAAAACCAAGAAAAGAGCTCCGAGACGTGGAAGAAAGAAAGCCACCATACAAGCATCAGAAGGGGAAACACAAGAAGGTCAAGTGAGCACTGAAGAAGATGAATCCCCTGAAGGGACTAAGAAAATAAAAAGGAGGGGCCGCAAGAAAGCTGCAACTACTGCAAGCTCATCGGAAGAGAAGGACAAAGCAAAAGAACCAAAGAAGAGGGGCAGAAGAAAAGTTAAGACTGTAGAGGAATTGAGTGACAATGAAGGGGAAGATCTGGGTGAAGATCTAGTGCCCTCCAATGACAGGCAGGAGAAAATTTCAGCAAATGACCTAGAAAGTAAAATAGCAGCATTGCTATTAGAAGATACTGATGATAATGATATTAACAATTTAATCCCTCTTGTGTGCTGCTTTGGGCCTGCTAAGTACTCATTTATTCCTTCTGGAAGACCTGCTAATAGGCTGATAGATCATGAAATTCATGAGGGAATGAAAGACATGTTCTGGTCTCCAGATCAATTTGTAAGGGCACCAGGAGGATCATCATCCAATGTTGCTCTTGCTTTAGCAGCTTCTGGTGGCCGGGTTGAATTCATGGGAAAACTAGGCGATGATGATTATGGTCAAAGTACATTATATCACTTGAACGTCAATGGAGTTCAAACTCGGGCAATTAAAATGGACCCTTCAGCGTTTACTGCCATGTCCTTGATGAAGGTCACAGGTAGGGGTAGCTTGAAAATGAGCTGTGCTAAACCTTGTGCAGAGGATTGTTTTGTCCAAACTGATATCAACCCAGCTGTTTTAAAAGAGGCTAAGATGTTTTACTACAATTCTTCAGCTTTGCTTGAGCCCACGACACGATCATCATTGTCGAAAGCAATTGAGGTTTCCAAGAAATTTGGTGGTGTAACATTCTTTGATCTCAATCTTCCACTGCCATTATGGTCGTCCAGTAAGGAGACCAAGTCACTTGTCAAGGAAGCATGGGAAGCTGCTGATATTATTGAAATCACTAAGCAGGAACTTGAGTTCTTGTGTGGCATTAAACCATCTGAGAAATTTGGTACGAAGGATAATGATAAATCCAAATTCACTCACTACAGCCCAGAAGTTGTTACGAAATTGTGGCATGAAAATCTCAAGGTCCTTTTTGTGACAAACGGCACTTCTAAGATTCATTATTACACAAAAGAGCATGATGGCTGGGTTCGTGGCACAGAAGATGCACCAATTACTCCTTTCACCGGTGACATGTCACAATCAGGTGACGCCATTGTTGCAGCTCTGATGAAGATGCTGGCAATTAACCCTCACCTGGTCACTGACAAGGATTACTTGCATACTGCAATGAAACATGCTATTACATGTGGTGTCATTGACCAGTGGTTACTTGCACGAGAACGAGGGTTCCTTCCCAGAGAAAGAGCAGATCCAACCAGTGAACAGTTTGGAGTGAGATTCGTCACAGAGAAGGAATACCGTACACTTCCTGATTCCATACATACAGAGGATTCATCAGAGAGTGAGCTTTTATATGTGGAGTGAACAAAGAAAATGCAGAGGCATGTAATTTTCAATAGAGGGAAAAACCTACAGACATCTGGTGATTTTTCTTGAAGCACCATTTATGGGAACTGCTGCTCTTAACTGGAGTTGGTATTTAGAAGAACTAGTTGTAACCTGCAGGCTCTAGGTTTATTTTTTAACACAGGGTAATAGCCTCAAGATAAACTAACACATCTGTATTGTCAAATTTTGGGTTAAGATTTTGTTGTAAATGTGCATGATTATGGGTGTCAGAAATTTTTCCTAGAACAGATGGATGCTAATTATATATTTCACTGCAACTCCACTATTCTACC
CDS Sequence
  • >LOC_Os03g40550.1
    ATGCACCGAATGGCTTCTCTTCTTCTCCCCCCGCAGTTTCTTTGCTCCCTGCCTTGTAGTACCAACTCAATCAGGAGCCATTTACATTATAAGCCCCACTTCTTGGGAAACATTATGACTAAGCCTAAAGCTAAAATGAGGCTGCTCAACCGAAACGTAAGTTCCATGGCAAAGAAGAGCTCTCAAGATGTAGCAGAAGGTTCAAGTGATGATGAGAGTGACGGCGAGACATCAAAAACCAAGAAAAGAGCTCCGAGACGTGGAAGAAAGAAAGCCACCATACAAGCATCAGAAGGGGAAACACAAGAAGGTCAAGTGAGCACTGAAGAAGATGAATCCCCTGAAGGGACTAAGAAAATAAAAAGGAGGGGCCGCAAGAAAGCTGCAACTACTGCAAGCTCATCGGAAGAGAAGGACAAAGCAAAAGAACCAAAGAAGAGGGGCAGAAGAAAAGTTAAGACTGTAGAGGAATTGAGTGACAATGAAGGGGAAGATCTGGGTGAAGATCTAGTGCCCTCCAATGACAGGCAGGAGAAAATTTCAGCAAATGACCTAGAAAGTAAAATAGCAGCATTGCTATTAGAAGATACTGATGATAATGATATTAACAATTTAATCCCTCTTGTGTGCTGCTTTGGGCCTGCTAAGTACTCATTTATTCCTTCTGGAAGACCTGCTAATAGGCTGATAGATCATGAAATTCATGAGGGAATGAAAGACATGTTCTGGTCTCCAGATCAATTTGTAAGGGCACCAGGAGGATCATCATCCAATGTTGCTCTTGCTTTAGCAGCTTCTGGTGGCCGGGTTGAATTCATGGGAAAACTAGGCGATGATGATTATGGTCAAAGTACATTATATCACTTGAACGTCAATGGAGTTCAAACTCGGGCAATTAAAATGGACCCTTCAGCGTTTACTGCCATGTCCTTGATGAAGGTCACAGGTAGGGGTAGCTTGAAAATGAGCTGTGCTAAACCTTGTGCAGAGGATTGTTTTGTCCAAACTGATATCAACCCAGCTGTTTTAAAAGAGGCTAAGATGTTTTACTACAATTCTTCAGCTTTGCTTGAGCCCACGACACGATCATCATTGTCGAAAGCAATTGAGGTTTCCAAGAAATTTGGTGGTGTAACATTCTTTGATCTCAATCTTCCACTGCCATTATGGTCGTCCAGTAAGGAGACCAAGTCACTTGTCAAGGAAGCATGGGAAGCTGCTGATATTATTGAAATCACTAAGCAGGAACTTGAGTTCTTGTGTGGCATTAAACCATCTGAGAAATTTGGTACGAAGGATAATGATAAATCCAAATTCACTCACTACAGCCCAGAAGTTGTTACGAAATTGTGGCATGAAAATCTCAAGGTCCTTTTTGTGACAAACGGCACTTCTAAGATTCATTATTACACAAAAGAGCATGATGGCTGGGTTCGTGGCACAGAAGATGCACCAATTACTCCTTTCACCGGTGACATGTCACAATCAGGTGACGCCATTGTTGCAGCTCTGATGAAGATGCTGGCAATTAACCCTCACCTGGTCACTGACAAGGATTACTTGCATACTGCAATGAAACATGCTATTACATGTGGTGTCATTGACCAGTGGTTACTTGCACGAGAACGAGGGTTCCTTCCCAGAGAAAGAGCAGATCCAACCAGTGAACAGTTTGGAGTGAGATTCGTCACAGAGAAGGAATACCGTACACTTCCTGATTCCATACATACAGAGGATTCATCAGAGAGTGAGCTTTTATATGTGGAGTGA
Protein Sequence
  • >LOC_Os03g40550.1
    MHRMASLLLPPQFLCSLPCSTNSIRSHLHYKPHFLGNIMTKPKAKMRLLNRNVSSMAKKSSQDVAEGSSDDESDGETSKTKKRAPRRGRKKATIQASEGETQEGQVSTEEDESPEGTKKIKRRGRKKAATTASSSEEKDKAKEPKKRGRRKVKTVEELSDNEGEDLGEDLVPSNDRQEKISANDLESKIAALLLEDTDDNDINNLIPLVCCFGPAKYSFIPSGRPANRLIDHEIHEGMKDMFWSPDQFVRAPGGSSSNVALALAASGGRVEFMGKLGDDDYGQSTLYHLNVNGVQTRAIKMDPSAFTAMSLMKVTGRGSLKMSCAKPCAEDCFVQTDINPAVLKEAKMFYYNSSALLEPTTRSSLSKAIEVSKKFGGVTFFDLNLPLPLWSSSKETKSLVKEAWEAADIIEITKQELEFLCGIKPSEKFGTKDNDKSKFTHYSPEVVTKLWHENLKVLFVTNGTSKIHYYTKEHDGWVRGTEDAPITPFTGDMSQSGDAIVAALMKMLAINPHLVTDKDYLHTAMKHAITCGVIDQWLLARERGFLPRERADPTSEQFGVRFVTEKEYRTLPDSIHTEDSSESELLYVE*