Gene Details:

Functional Descriptions:

  • Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice.
  • Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of OsPLS2.
  • This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence.
  • When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the OsPLS2 mutant sustained hypersensitive leaf senescence.
  • In consistence, light and ROS signal pathways in OsPLS2 were activated by down-regulation of phytochrome genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence.
  • Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice.
  • Shading and light filtration studies showed that the OsPLS2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light.
  • The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase.
  • Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g38990.1
    GATTACGCCGTGGACGAGACGAGTGAGAGGAGGAGAGCGGCCATGGCGGGGCGGAGTGGCGGCGGCGGCGGCGGGAGCTCCGGGAAGAGCGGGACGGGGAGGATGGTGTCGCTGCAGGAGTTCGTCTCGTCCATGGCGCCTCTCATAGACCTGGAGAAGGCGGCGGAGATATCGGCGGAGTCGGCGACGAGCTCGAAGACCCTCGAGAGGAGGGGTTGCGTCATGGCCAACCTCAAGTGCACCGACGCCCAGACGGGGCTGATGGGGAAGACGCTCCTGGAGTTCCAACCCAACAAGGGCGACGTGCTTCCACCTCACAAGTTTGGAACACACGATGTGGTTGCTCTGAAGCCAAATAAGGCAGATGCTGGATCTGCTGCTCTTGGGCAAGGTGTAGTTTATCGCTTGAAGGATTCTTCCATTACTGTTGCCTTTGATGACATTCCTGAAGATGGATTAAATAGCCCTCTCCGCCTTGAAAAGCTTGCAAATGAGGTGACATACCGCAGGATGAAGGATGCACTAGTTCAACTTAGTAAGGGCATTCAAACAGGCCCTTCAGCAAACCTTATTCCTGTTTTGTTTGGAGAAAACCCACCTATGAGTTCCAAGGATGTTGCGAAGTTCAGTCCATTTAACAAGAATTTGGATGAGTCACAGAAAGATGCTATCTCGAAGGCCCTCAGGTCAAGGGATGTTTTCTTGCTTCATGGACCTCCTGGCACTGGGAAGACAACAACTATCATTGAGATAATATTGCAGGAGGTGAAACGTGGATCAAAGATTCTTGCTTGTGCTGCTTCTAACATCGCCGTGGATAACATTGTTGAGCGACTCTCCCGATATAGAACCAAATTGGTGAGGTTAGGCCATCCTGCTCGATTACTACCCCAAGTGTTGGACAGTGCTCTTGACGCACAGGTATTGCGAGCAGATAACAGCAGCTTAGCAGGAGATATTCGCAAAGAAATGAAGGTGCTTAATAGCAAATTGCTGAAAGCTAAGGATAAGAATACAAAAAGGGACATCAGGAAAGAGCTCAGAACCCTTGCCAAAGAAGAGAGAAAACGACAACAGCTTGCTGTTGCTGATGTGATAAAAAATGCAGATGTTGTACTGTCGACTTTGACTGGTGCATCTTCCAAAAAGCTAGATGGTATTACATTTGATTTGGTCATTATTGATGAAGCTGCTCAGGCACTTGAGATGGCATGTTGGATAGCCTTATTGAAGGGTCCAAGATGTGTGCTTGCTGGAGACCATCTTCAACTTCCTCCGACTATCCAAAGTGCTGAGGCTGAGAAGAAGGGGATGGGAAAGACACTCTTTGAACGCCTTACTGAAGCTTATGGAGATCAAATTACATCCATGCTCACTATCCAGTACAGAATGCATGAGCTCATAATGAATTGGTCATCTAAAGAACTTTATAATAACAAGATCAAAGCTCATTCTAGTGTTGCTGATCATATGCTCTATGACATTGAAGAAGTGAAAAGATCTTCTTCCACAGAGCCGACTATCATACTAATTGACACTACTGGGTGTGACATGGAAGAAGTAAAAGATGAGGAGGAGAGCACTATGAATGAGGGTGAGGCTGCAGTATCCATTGCTCATGCCAAGCTGCTTGTTGAGAGTGGTGTCCGTGCATCTGATATTGGAATAATTACACCTTATGCTGCACAGGTAACCTGTCTGAAGATGATGAGAAACAAAGATACTAAACTGAAGGACTTGGAGATATCGACAGTTGATGGGTTCCAGGGTAGGGAGAAAGAAGCCATCATCATTTCAATGGTCAGATCCAACTCAAAGAAAGAGGTCGGATTCTTAAGTGATCATAGACGAATGAATGTTGCTGTAACACGAGCCAGAAGGCAGTGCTGCCTAGTGTGCGATGTAGAGACCGTAAGCAACGACAAATTCTTGAAACGCTTGGTTGAGTACTTTGAGGAGAACGGGGAGTACTTGAGTGCATCAGAATACCAGAGCTGATGACTTGGGGTGTTAACCCTTAACCGTGATAGCCCGTGCTTATCGCAAAGATAGTGAAGAGATATATCCAAACGATGCTTGTGATAGGAACATCGTGCACTTCAAACTGGTGCAATATGGCCAATGGAGATACTGTCATTCGACTTTTATAGATTGGAAACAGATCGCTGTGAGACTGAACAGATAATTAGCTCAGGAGTGGATCAATTTCGTCTTGACTGGCCCTGTTCTGGTCTCTTCTTCTGTGGCTTATGGAAATGAAACCTCAGTTATGTGTTTTTATATGTCCGTTCTTTCAGCTGATGTACAATGATAGAAGACTAATTTTATTCATCCATGTGCAAATCTACCATAGTTTTTTGCATGGAGTTTTGAAACTTGGGTCCACAAAAATAGTAATAATAGGATAGGAGGTTGACTGTACTGAACTTCGCATGATAATTAATCTGTATCTCATCCATCTTGCTTATTTTCACAATGTCGCCCAAAATACCAAA
CDS Sequence
  • >LOC_Os03g38990.1
    ATGGCGGGGCGGAGTGGCGGCGGCGGCGGCGGGAGCTCCGGGAAGAGCGGGACGGGGAGGATGGTGTCGCTGCAGGAGTTCGTCTCGTCCATGGCGCCTCTCATAGACCTGGAGAAGGCGGCGGAGATATCGGCGGAGTCGGCGACGAGCTCGAAGACCCTCGAGAGGAGGGGTTGCGTCATGGCCAACCTCAAGTGCACCGACGCCCAGACGGGGCTGATGGGGAAGACGCTCCTGGAGTTCCAACCCAACAAGGGCGACGTGCTTCCACCTCACAAGTTTGGAACACACGATGTGGTTGCTCTGAAGCCAAATAAGGCAGATGCTGGATCTGCTGCTCTTGGGCAAGGTGTAGTTTATCGCTTGAAGGATTCTTCCATTACTGTTGCCTTTGATGACATTCCTGAAGATGGATTAAATAGCCCTCTCCGCCTTGAAAAGCTTGCAAATGAGGTGACATACCGCAGGATGAAGGATGCACTAGTTCAACTTAGTAAGGGCATTCAAACAGGCCCTTCAGCAAACCTTATTCCTGTTTTGTTTGGAGAAAACCCACCTATGAGTTCCAAGGATGTTGCGAAGTTCAGTCCATTTAACAAGAATTTGGATGAGTCACAGAAAGATGCTATCTCGAAGGCCCTCAGGTCAAGGGATGTTTTCTTGCTTCATGGACCTCCTGGCACTGGGAAGACAACAACTATCATTGAGATAATATTGCAGGAGGTGAAACGTGGATCAAAGATTCTTGCTTGTGCTGCTTCTAACATCGCCGTGGATAACATTGTTGAGCGACTCTCCCGATATAGAACCAAATTGGTGAGGTTAGGCCATCCTGCTCGATTACTACCCCAAGTGTTGGACAGTGCTCTTGACGCACAGGTATTGCGAGCAGATAACAGCAGCTTAGCAGGAGATATTCGCAAAGAAATGAAGGTGCTTAATAGCAAATTGCTGAAAGCTAAGGATAAGAATACAAAAAGGGACATCAGGAAAGAGCTCAGAACCCTTGCCAAAGAAGAGAGAAAACGACAACAGCTTGCTGTTGCTGATGTGATAAAAAATGCAGATGTTGTACTGTCGACTTTGACTGGTGCATCTTCCAAAAAGCTAGATGGTATTACATTTGATTTGGTCATTATTGATGAAGCTGCTCAGGCACTTGAGATGGCATGTTGGATAGCCTTATTGAAGGGTCCAAGATGTGTGCTTGCTGGAGACCATCTTCAACTTCCTCCGACTATCCAAAGTGCTGAGGCTGAGAAGAAGGGGATGGGAAAGACACTCTTTGAACGCCTTACTGAAGCTTATGGAGATCAAATTACATCCATGCTCACTATCCAGTACAGAATGCATGAGCTCATAATGAATTGGTCATCTAAAGAACTTTATAATAACAAGATCAAAGCTCATTCTAGTGTTGCTGATCATATGCTCTATGACATTGAAGAAGTGAAAAGATCTTCTTCCACAGAGCCGACTATCATACTAATTGACACTACTGGGTGTGACATGGAAGAAGTAAAAGATGAGGAGGAGAGCACTATGAATGAGGGTGAGGCTGCAGTATCCATTGCTCATGCCAAGCTGCTTGTTGAGAGTGGTGTCCGTGCATCTGATATTGGAATAATTACACCTTATGCTGCACAGGTAACCTGTCTGAAGATGATGAGAAACAAAGATACTAAACTGAAGGACTTGGAGATATCGACAGTTGATGGGTTCCAGGGTAGGGAGAAAGAAGCCATCATCATTTCAATGGTCAGATCCAACTCAAAGAAAGAGGTCGGATTCTTAAGTGATCATAGACGAATGAATGTTGCTGTAACACGAGCCAGAAGGCAGTGCTGCCTAGTGTGCGATGTAGAGACCGTAAGCAACGACAAATTCTTGAAACGCTTGGTTGAGTACTTTGAGGAGAACGGGGAGTACTTGAGTGCATCAGAATACCAGAGCTGA
Protein Sequence
  • >LOC_Os03g38990.1
    MAGRSGGGGGGSSGKSGTGRMVSLQEFVSSMAPLIDLEKAAEISAESATSSKTLERRGCVMANLKCTDAQTGLMGKTLLEFQPNKGDVLPPHKFGTHDVVALKPNKADAGSAALGQGVVYRLKDSSITVAFDDIPEDGLNSPLRLEKLANEVTYRRMKDALVQLSKGIQTGPSANLIPVLFGENPPMSSKDVAKFSPFNKNLDESQKDAISKALRSRDVFLLHGPPGTGKTTTIIEIILQEVKRGSKILACAASNIAVDNIVERLSRYRTKLVRLGHPARLLPQVLDSALDAQVLRADNSSLAGDIRKEMKVLNSKLLKAKDKNTKRDIRKELRTLAKEERKRQQLAVADVIKNADVVLSTLTGASSKKLDGITFDLVIIDEAAQALEMACWIALLKGPRCVLAGDHLQLPPTIQSAEAEKKGMGKTLFERLTEAYGDQITSMLTIQYRMHELIMNWSSKELYNNKIKAHSSVADHMLYDIEEVKRSSSTEPTIILIDTTGCDMEEVKDEEESTMNEGEAAVSIAHAKLLVESGVRASDIGIITPYAAQVTCLKMMRNKDTKLKDLEISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDHRRMNVAVTRARRQCCLVCDVETVSNDKFLKRLVEYFEENGEYLSASEYQS*