Information report for OsHAK21;qSE3
Gene Details
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Functional Descriptions
- Our analyses of subcellular localizations and expression patterns showed that OsHAK21 was localized in the plasma membrane, and expressed in xylem parenchyma and individual endodermal cells (putative passage cells).
- Here, we report a significant inducement of OsHAK21 expression by high salinity treatment, and provide genetic evidence of the involvement of OsHAK21 in rice salt tolerance.
- The potassium transporter OsHAK21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice.
- Disruption of OsHAK21 rendered plants sensitive to salt stress.
- These results demonstrate that OsHAK21 may mediate K(+) absorption by the plasma membrane, and play crucial roles in the maintenance of the Na(+) /K(+) homeostasis in rice under salt stress.
- Further functional characterizations of OsHAK21 in K(+) uptake-deficient yeast and Arabidopsis revealed that OsHAK21 possesses K(+) transporter activity.
- In this study, we mapped one major QTL qSE3 for seed germination and seedling establishment under salinity stress in rice.
- All of these seed physiological changes modulated by qSE3 might contribute to seed germination and seedling establishment under salinity stress.
- This study provides important insights into the roles of qSE3 in seed germination and seedling establishment under salinity stress and facilitates the practical use of qSE3 in rice breeding.
- Based on analysis of single-nucleotide polymorphism data of rice accessions, we identified a HAP3 haplotype of qSE3 that was positively correlated with seed germination under salinity stress.
- To understand the molecular basis of this QTL, we isolated qSE3 by map-based cloning and found that it encodes a K+ transporter gene, OsHAK21.
- The expression of qSE3 was significantly up-regulated by salinity stress in germinating seeds.
- Physiological analysis suggested that qSE3 significantly increased K+ and Na+ uptake in germinating seeds under salinity stress, resulting in increased abscisic acid (ABA) biosynthesis and activated ABA signaling responses.
- Furthermore, qSE3 significantly decreased the H2 O2 level in germinating seeds under salinity stress.
- Haplotype analysis revealed four key variations associated with salt tolerance, including an SNP (+1605 bp) within OsABCI7 exon, an SSR (-1231 bp) within OsHAK21 promoter, an indel site at OsNHX1 promoter (-822 bp), and an SNP (-1866 bp) within OsAKT2 promoter.
Functional Keywords
- xylem , salinity , salt , salt-tolerance , salt-stress , tolerance , stress , homeostasis , transporter , plasma-membrane , potassium , xylem-parenchyma , seedling , seed , map-based-cloning , seed-germination , ABA , salinity-stress , breeding , abscisic-acid
Literature and News
- The potassium transporter OsHAK21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice . DOI: 10.1111/pce.12586 ; PMID: 26046379
- A quantitative trait locus, qSE3, promotes seed germination and seedling establishment under salinity stress in rice . DOI: 10.1111/tpj.14181 ; PMID: 30537381
- Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress . DOI: 10.1186/s12870-020-02820-7 ; PMID: 33422012
- Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances . DOI: 10.3389/fpls.2023.1095929 ; PMID: 37008489
Gene Resources
- UniProt: Q75G84
- EMBL: AC146468, AP008209, AP014959
- AlphaFoldDB: Q75G84
- EnsemblPlants: Os03t0576200-01
- Gramene: Os03t0576200-01
- KEGG: dosa:Os03g0576200
- Orthologous matrix: DHDSWGT
- InterPro: IPR003855, IPR053951, IPR053952
- PANTHER: PTHR30540, PTHR30540:SF28
- eggNOG: ENOG502QPSA
Sequences
cDNA Sequence
- >LOC_Os03g37930.1
ATGGATCCAGGAGTTGAGAAGAAGAAGCAGCAGATGGAGCTCGTCGACGTCGAGAGCGGCGGCCTTCCCGTCGAGCGTCAGGACTCGCTGTTCCGGGAGGCGGTGAGAGCTGAGCACGCCGGCGCCGCTCACTGGGACGAACAGGATAGCTGGGGGAGGACGATGAGTCTGGCGTTCCAGTGCGTGGGGATCCTGTACGGCGACATTGGGACGTCGTCGTTGTACGTCTACTCCAGCACTTTTGAGCACGGCATTGGGCACCCCGATGACGTCGTCGGGGTGCTCTCCCTCATCGTCTACAGCTTCATGCTCTTCACCGTCATCAAGATCGTCTTCGTCGCCCTGCATGCCAACGACCATGGTGACGGTGGGACATTTGCACTCTACTCGTTAATTTCACGGCACGCGAAGGTGAGCCTGATCCCTAACCATCAGGCGGAAGATGAGCTGATCTCCGGGTATAGCAGTAGCGGGAAGCCATCGGCGACTCTGAGGAGAGCTCACTGGTTGAAACAGCTGCTCGAGGCGAGCAAGGCGGCCAAGATTTCTCTCTTCCTGCTCACCATCCTTGCGATCGCCATGGTCATCAGCGACGCTGTGCTAACGCCTCCCATTTCTGTACTGTCAGCCGTAGGTGGGTTGAGAGAGAAGGTACCTCATCTTACAACAGATCAAATAGTGTGGATAACAGTGGCAATTTTGGTAGTCTTGTTTGCAATCCAGCGCTACGGGACTGATAAAGTTGGCTACTCTTTTGCACCAATCATTCTCTTGTGGCTGCTCTTGATTGGAGCAACTGGTCTCTATAACCTGATCAAGCATGACATCAGTGTCTTGAGGGCCTTCAACCCAAAATACATCATCGACTATTTCCGTAGGAACAAGAAGGAAGGATGGGTCTCGCTTGGAAGTATTCTCCTTTGCTTCACAGGATCAGAAGCACTTTTTGCTAATCTAGGATACTTCAGCATCAGATCAATCCAGCTGAGCTTTAGTTTTGCCTTACTTCCATCGGTATTACTCACGTACATCGGACAAGCAGCTTTCCTGAGTAAGAACCCGAAGAATGTTGCCAATACCTTCTTTGCCGCTACTCCAATTTCTTTGTTCTGGCCTACGTTCATCATGGCGATAGCTGCATCCATAATAGGAAGCCAAGCAATGATATCTTGTGCCTTTGCAACTGTTTCTCATTTACAATCACTTAGTTGCTTCCCAAGGGTCAAGATATTGCACACATCAAAGCGATTTCCGGGCCAATTGTACATCCCTGGAGTAAACTTCTTGTTATGTGTGGCTGCTTGCGTTGTGACTGTTAGCTTCAAAACAACTGTTATTATAGGAAAGGCGCACGAAATTTGTGTCATCCTTGTGATGATCATCACAACATTACTAATGACAATAGTTATGCTCTTGGTATGGAAGATCAACATCTTGTGGGTTGCTCTGTTTTTTATCACATTCACATCAACGGAGGCTGTCTACCTATCGTCAGTGCTATACAAGTTCACACATGGACCATATGTGCCTGTGGCTATGTCGGTAGTTCTAATGGTGGTGATGATCGTGTGGCACTACGTGCATGTGAAAAGATACAAGTATGAATTGGAACACACAGTATCCACCGACAAGGTGAAAGAAATGCTGGAGAGCCATGATCTGAAGAGGGTCCGTGGCGTAGCCCTGTTCTACACTGAACTAGTCCAAGGCATCCCGCCCATATTCCCGCACCTCATCGAGAAGATCCCAACGATTCATTCTGTTCTTGTCTTCATCTCTATCAAGCATTTGCCAGTACCCCATGTTGACACCTCGGAGCGATTCCTCTTCCGTCAAGTAGAGCTAAAGGACTACAAAGTGTTTCGATGTGTGGCACGATATGGATATCGTGATTCCCTCGAGGAGGCCAAGGACTTTGTTGTTACCCTTCTTGAGAACCTTCAGGATTATATAAGGGATGTTAATCTCTACACCGATGAGCCACACACCATTAGTGCCCATAGTTCTTGCAACCATAGCTTTTCTCGGGAGAAGCCTTCTGGACGGTACGCGGTACATGCCGAGGATATGCTTACACCAATTGAATCCTTCTCTGAGATCACAGCTCTATCTAACTATGGAAGCGACCGTCTACCACACTTCAAGGCAAGTAAGATGAATATGGAGGAGTTGGCGAAGATTGAGCAAGAGCAGATGTTCATTGAGAAGGAGATGGAAAAAGGTGTTGTATACATACTTGGAGAAACCGAGGTGGTTGTAAGGCCTCACTCATCGCTTCTTAAGAAGATAGTAGTGAACTACGTCTACTCTTTCTTAAGAAAGAACTTCGTGCAAGGGCAGAAGATGCTATTTATTCCCCATAGGCAATTGCTTAAAGTAGGGATCTCATATGAGATTTAGGTTGTAAGAAATGGAAATATACATGATATGGTGTTGGTTGGTGTCAAGATGAATGTATAAATATTTGTCTTTGTAAGGTAATATTAAAACTGTAACATGTAGTCAGATGGTTAAATGATAAGCTATGATGCTGCATAGGAACGAGTTGTTCCATTGTGGCTAGCTTTTGAGGTTAGGGGATTTTCTTGTACTGCACACTACTTCAAATGTTTACTTGACATACAA
CDS Sequence
- >LOC_Os03g37930.1
ATGGATCCAGGAGTTGAGAAGAAGAAGCAGCAGATGGAGCTCGTCGACGTCGAGAGCGGCGGCCTTCCCGTCGAGCGTCAGGACTCGCTGTTCCGGGAGGCGGTGAGAGCTGAGCACGCCGGCGCCGCTCACTGGGACGAACAGGATAGCTGGGGGAGGACGATGAGTCTGGCGTTCCAGTGCGTGGGGATCCTGTACGGCGACATTGGGACGTCGTCGTTGTACGTCTACTCCAGCACTTTTGAGCACGGCATTGGGCACCCCGATGACGTCGTCGGGGTGCTCTCCCTCATCGTCTACAGCTTCATGCTCTTCACCGTCATCAAGATCGTCTTCGTCGCCCTGCATGCCAACGACCATGGTGACGGTGGGACATTTGCACTCTACTCGTTAATTTCACGGCACGCGAAGGTGAGCCTGATCCCTAACCATCAGGCGGAAGATGAGCTGATCTCCGGGTATAGCAGTAGCGGGAAGCCATCGGCGACTCTGAGGAGAGCTCACTGGTTGAAACAGCTGCTCGAGGCGAGCAAGGCGGCCAAGATTTCTCTCTTCCTGCTCACCATCCTTGCGATCGCCATGGTCATCAGCGACGCTGTGCTAACGCCTCCCATTTCTGTACTGTCAGCCGTAGGTGGGTTGAGAGAGAAGGTACCTCATCTTACAACAGATCAAATAGTGTGGATAACAGTGGCAATTTTGGTAGTCTTGTTTGCAATCCAGCGCTACGGGACTGATAAAGTTGGCTACTCTTTTGCACCAATCATTCTCTTGTGGCTGCTCTTGATTGGAGCAACTGGTCTCTATAACCTGATCAAGCATGACATCAGTGTCTTGAGGGCCTTCAACCCAAAATACATCATCGACTATTTCCGTAGGAACAAGAAGGAAGGATGGGTCTCGCTTGGAAGTATTCTCCTTTGCTTCACAGGATCAGAAGCACTTTTTGCTAATCTAGGATACTTCAGCATCAGATCAATCCAGCTGAGCTTTAGTTTTGCCTTACTTCCATCGGTATTACTCACGTACATCGGACAAGCAGCTTTCCTGAGTAAGAACCCGAAGAATGTTGCCAATACCTTCTTTGCCGCTACTCCAATTTCTTTGTTCTGGCCTACGTTCATCATGGCGATAGCTGCATCCATAATAGGAAGCCAAGCAATGATATCTTGTGCCTTTGCAACTGTTTCTCATTTACAATCACTTAGTTGCTTCCCAAGGGTCAAGATATTGCACACATCAAAGCGATTTCCGGGCCAATTGTACATCCCTGGAGTAAACTTCTTGTTATGTGTGGCTGCTTGCGTTGTGACTGTTAGCTTCAAAACAACTGTTATTATAGGAAAGGCGCACGAAATTTGTGTCATCCTTGTGATGATCATCACAACATTACTAATGACAATAGTTATGCTCTTGGTATGGAAGATCAACATCTTGTGGGTTGCTCTGTTTTTTATCACATTCACATCAACGGAGGCTGTCTACCTATCGTCAGTGCTATACAAGTTCACACATGGACCATATGTGCCTGTGGCTATGTCGGTAGTTCTAATGGTGGTGATGATCGTGTGGCACTACGTGCATGTGAAAAGATACAAGTATGAATTGGAACACACAGTATCCACCGACAAGGTGAAAGAAATGCTGGAGAGCCATGATCTGAAGAGGGTCCGTGGCGTAGCCCTGTTCTACACTGAACTAGTCCAAGGCATCCCGCCCATATTCCCGCACCTCATCGAGAAGATCCCAACGATTCATTCTGTTCTTGTCTTCATCTCTATCAAGCATTTGCCAGTACCCCATGTTGACACCTCGGAGCGATTCCTCTTCCGTCAAGTAGAGCTAAAGGACTACAAAGTGTTTCGATGTGTGGCACGATATGGATATCGTGATTCCCTCGAGGAGGCCAAGGACTTTGTTGTTACCCTTCTTGAGAACCTTCAGGATTATATAAGGGATGTTAATCTCTACACCGATGAGCCACACACCATTAGTGCCCATAGTTCTTGCAACCATAGCTTTTCTCGGGAGAAGCCTTCTGGACGGTACGCGGTACATGCCGAGGATATGCTTACACCAATTGAATCCTTCTCTGAGATCACAGCTCTATCTAACTATGGAAGCGACCGTCTACCACACTTCAAGGCAAGTAAGATGAATATGGAGGAGTTGGCGAAGATTGAGCAAGAGCAGATGTTCATTGAGAAGGAGATGGAAAAAGGTGTTGTATACATACTTGGAGAAACCGAGGTGGTTGTAAGGCCTCACTCATCGCTTCTTAAGAAGATAGTAGTGAACTACGTCTACTCTTTCTTAAGAAAGAACTTCGTGCAAGGGCAGAAGATGCTATTTATTCCCCATAGGCAATTGCTTAAAGTAGGGATCTCATATGAGATTTAG
Protein Sequence
- >LOC_Os03g37930.1
MDPGVEKKKQQMELVDVESGGLPVERQDSLFREAVRAEHAGAAHWDEQDSWGRTMSLAFQCVGILYGDIGTSSLYVYSSTFEHGIGHPDDVVGVLSLIVYSFMLFTVIKIVFVALHANDHGDGGTFALYSLISRHAKVSLIPNHQAEDELISGYSSSGKPSATLRRAHWLKQLLEASKAAKISLFLLTILAIAMVISDAVLTPPISVLSAVGGLREKVPHLTTDQIVWITVAILVVLFAIQRYGTDKVGYSFAPIILLWLLLIGATGLYNLIKHDISVLRAFNPKYIIDYFRRNKKEGWVSLGSILLCFTGSEALFANLGYFSIRSIQLSFSFALLPSVLLTYIGQAAFLSKNPKNVANTFFAATPISLFWPTFIMAIAASIIGSQAMISCAFATVSHLQSLSCFPRVKILHTSKRFPGQLYIPGVNFLLCVAACVVTVSFKTTVIIGKAHEICVILVMIITTLLMTIVMLLVWKINILWVALFFITFTSTEAVYLSSVLYKFTHGPYVPVAMSVVLMVVMIVWHYVHVKRYKYELEHTVSTDKVKEMLESHDLKRVRGVALFYTELVQGIPPIFPHLIEKIPTIHSVLVFISIKHLPVPHVDTSERFLFRQVELKDYKVFRCVARYGYRDSLEEAKDFVVTLLENLQDYIRDVNLYTDEPHTISAHSSCNHSFSREKPSGRYAVHAEDMLTPIESFSEITALSNYGSDRLPHFKASKMNMEELAKIEQEQMFIEKEMEKGVVYILGETEVVVRPHSSLLKKIVVNYVYSFLRKNFVQGQKMLFIPHRQLLKVGISYEI*