Gene Details:

Functional Descriptions:

  • Our analyses of subcellular localizations and expression patterns showed that OsHAK21 was localized in the plasma membrane, and expressed in xylem parenchyma and individual endodermal cells (putative passage cells).
  • Here, we report a significant inducement of OsHAK21 expression by high salinity treatment, and provide genetic evidence of the involvement of OsHAK21 in rice salt tolerance.
  • The potassium transporter OsHAK21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice.
  • Disruption of OsHAK21 rendered plants sensitive to salt stress.
  • These results demonstrate that OsHAK21 may mediate K(+) absorption by the plasma membrane, and play crucial roles in the maintenance of the Na(+) /K(+) homeostasis in rice under salt stress.
  • Further functional characterizations of OsHAK21 in K(+) uptake-deficient yeast and Arabidopsis revealed that OsHAK21 possesses K(+) transporter activity.
  • In this study, we mapped one major QTL qSE3 for seed germination and seedling establishment under salinity stress in rice.
  • All of these seed physiological changes modulated by qSE3 might contribute to seed germination and seedling establishment under salinity stress.
  • This study provides important insights into the roles of qSE3 in seed germination and seedling establishment under salinity stress and facilitates the practical use of qSE3 in rice breeding.
  • Based on analysis of single-nucleotide polymorphism data of rice accessions, we identified a HAP3 haplotype of qSE3 that was positively correlated with seed germination under salinity stress.
  • To understand the molecular basis of this QTL, we isolated qSE3 by map-based cloning and found that it encodes a K+ transporter gene, OsHAK21.
  • The expression of qSE3 was significantly up-regulated by salinity stress in germinating seeds.
  • Physiological analysis suggested that qSE3 significantly increased K+ and Na+ uptake in germinating seeds under salinity stress, resulting in increased abscisic acid (ABA) biosynthesis and activated ABA signaling responses.
  • Furthermore, qSE3 significantly decreased the H2 O2 level in germinating seeds under salinity stress.
  • Haplotype analysis revealed four key variations associated with salt tolerance, including an SNP (+1605 bp) within OsABCI7 exon, an SSR (-1231 bp) within OsHAK21 promoter, an indel site at OsNHX1 promoter (-822 bp), and an SNP (-1866 bp) within OsAKT2 promoter.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g37930.1
    ATGGATCCAGGAGTTGAGAAGAAGAAGCAGCAGATGGAGCTCGTCGACGTCGAGAGCGGCGGCCTTCCCGTCGAGCGTCAGGACTCGCTGTTCCGGGAGGCGGTGAGAGCTGAGCACGCCGGCGCCGCTCACTGGGACGAACAGGATAGCTGGGGGAGGACGATGAGTCTGGCGTTCCAGTGCGTGGGGATCCTGTACGGCGACATTGGGACGTCGTCGTTGTACGTCTACTCCAGCACTTTTGAGCACGGCATTGGGCACCCCGATGACGTCGTCGGGGTGCTCTCCCTCATCGTCTACAGCTTCATGCTCTTCACCGTCATCAAGATCGTCTTCGTCGCCCTGCATGCCAACGACCATGGTGACGGTGGGACATTTGCACTCTACTCGTTAATTTCACGGCACGCGAAGGTGAGCCTGATCCCTAACCATCAGGCGGAAGATGAGCTGATCTCCGGGTATAGCAGTAGCGGGAAGCCATCGGCGACTCTGAGGAGAGCTCACTGGTTGAAACAGCTGCTCGAGGCGAGCAAGGCGGCCAAGATTTCTCTCTTCCTGCTCACCATCCTTGCGATCGCCATGGTCATCAGCGACGCTGTGCTAACGCCTCCCATTTCTGTACTGTCAGCCGTAGGTGGGTTGAGAGAGAAGGTACCTCATCTTACAACAGATCAAATAGTGTGGATAACAGTGGCAATTTTGGTAGTCTTGTTTGCAATCCAGCGCTACGGGACTGATAAAGTTGGCTACTCTTTTGCACCAATCATTCTCTTGTGGCTGCTCTTGATTGGAGCAACTGGTCTCTATAACCTGATCAAGCATGACATCAGTGTCTTGAGGGCCTTCAACCCAAAATACATCATCGACTATTTCCGTAGGAACAAGAAGGAAGGATGGGTCTCGCTTGGAAGTATTCTCCTTTGCTTCACAGGATCAGAAGCACTTTTTGCTAATCTAGGATACTTCAGCATCAGATCAATCCAGCTGAGCTTTAGTTTTGCCTTACTTCCATCGGTATTACTCACGTACATCGGACAAGCAGCTTTCCTGAGTAAGAACCCGAAGAATGTTGCCAATACCTTCTTTGCCGCTACTCCAATTTCTTTGTTCTGGCCTACGTTCATCATGGCGATAGCTGCATCCATAATAGGAAGCCAAGCAATGATATCTTGTGCCTTTGCAACTGTTTCTCATTTACAATCACTTAGTTGCTTCCCAAGGGTCAAGATATTGCACACATCAAAGCGATTTCCGGGCCAATTGTACATCCCTGGAGTAAACTTCTTGTTATGTGTGGCTGCTTGCGTTGTGACTGTTAGCTTCAAAACAACTGTTATTATAGGAAAGGCGCACGAAATTTGTGTCATCCTTGTGATGATCATCACAACATTACTAATGACAATAGTTATGCTCTTGGTATGGAAGATCAACATCTTGTGGGTTGCTCTGTTTTTTATCACATTCACATCAACGGAGGCTGTCTACCTATCGTCAGTGCTATACAAGTTCACACATGGACCATATGTGCCTGTGGCTATGTCGGTAGTTCTAATGGTGGTGATGATCGTGTGGCACTACGTGCATGTGAAAAGATACAAGTATGAATTGGAACACACAGTATCCACCGACAAGGTGAAAGAAATGCTGGAGAGCCATGATCTGAAGAGGGTCCGTGGCGTAGCCCTGTTCTACACTGAACTAGTCCAAGGCATCCCGCCCATATTCCCGCACCTCATCGAGAAGATCCCAACGATTCATTCTGTTCTTGTCTTCATCTCTATCAAGCATTTGCCAGTACCCCATGTTGACACCTCGGAGCGATTCCTCTTCCGTCAAGTAGAGCTAAAGGACTACAAAGTGTTTCGATGTGTGGCACGATATGGATATCGTGATTCCCTCGAGGAGGCCAAGGACTTTGTTGTTACCCTTCTTGAGAACCTTCAGGATTATATAAGGGATGTTAATCTCTACACCGATGAGCCACACACCATTAGTGCCCATAGTTCTTGCAACCATAGCTTTTCTCGGGAGAAGCCTTCTGGACGGTACGCGGTACATGCCGAGGATATGCTTACACCAATTGAATCCTTCTCTGAGATCACAGCTCTATCTAACTATGGAAGCGACCGTCTACCACACTTCAAGGCAAGTAAGATGAATATGGAGGAGTTGGCGAAGATTGAGCAAGAGCAGATGTTCATTGAGAAGGAGATGGAAAAAGGTGTTGTATACATACTTGGAGAAACCGAGGTGGTTGTAAGGCCTCACTCATCGCTTCTTAAGAAGATAGTAGTGAACTACGTCTACTCTTTCTTAAGAAAGAACTTCGTGCAAGGGCAGAAGATGCTATTTATTCCCCATAGGCAATTGCTTAAAGTAGGGATCTCATATGAGATTTAGGTTGTAAGAAATGGAAATATACATGATATGGTGTTGGTTGGTGTCAAGATGAATGTATAAATATTTGTCTTTGTAAGGTAATATTAAAACTGTAACATGTAGTCAGATGGTTAAATGATAAGCTATGATGCTGCATAGGAACGAGTTGTTCCATTGTGGCTAGCTTTTGAGGTTAGGGGATTTTCTTGTACTGCACACTACTTCAAATGTTTACTTGACATACAA
CDS Sequence
  • >LOC_Os03g37930.1
    ATGGATCCAGGAGTTGAGAAGAAGAAGCAGCAGATGGAGCTCGTCGACGTCGAGAGCGGCGGCCTTCCCGTCGAGCGTCAGGACTCGCTGTTCCGGGAGGCGGTGAGAGCTGAGCACGCCGGCGCCGCTCACTGGGACGAACAGGATAGCTGGGGGAGGACGATGAGTCTGGCGTTCCAGTGCGTGGGGATCCTGTACGGCGACATTGGGACGTCGTCGTTGTACGTCTACTCCAGCACTTTTGAGCACGGCATTGGGCACCCCGATGACGTCGTCGGGGTGCTCTCCCTCATCGTCTACAGCTTCATGCTCTTCACCGTCATCAAGATCGTCTTCGTCGCCCTGCATGCCAACGACCATGGTGACGGTGGGACATTTGCACTCTACTCGTTAATTTCACGGCACGCGAAGGTGAGCCTGATCCCTAACCATCAGGCGGAAGATGAGCTGATCTCCGGGTATAGCAGTAGCGGGAAGCCATCGGCGACTCTGAGGAGAGCTCACTGGTTGAAACAGCTGCTCGAGGCGAGCAAGGCGGCCAAGATTTCTCTCTTCCTGCTCACCATCCTTGCGATCGCCATGGTCATCAGCGACGCTGTGCTAACGCCTCCCATTTCTGTACTGTCAGCCGTAGGTGGGTTGAGAGAGAAGGTACCTCATCTTACAACAGATCAAATAGTGTGGATAACAGTGGCAATTTTGGTAGTCTTGTTTGCAATCCAGCGCTACGGGACTGATAAAGTTGGCTACTCTTTTGCACCAATCATTCTCTTGTGGCTGCTCTTGATTGGAGCAACTGGTCTCTATAACCTGATCAAGCATGACATCAGTGTCTTGAGGGCCTTCAACCCAAAATACATCATCGACTATTTCCGTAGGAACAAGAAGGAAGGATGGGTCTCGCTTGGAAGTATTCTCCTTTGCTTCACAGGATCAGAAGCACTTTTTGCTAATCTAGGATACTTCAGCATCAGATCAATCCAGCTGAGCTTTAGTTTTGCCTTACTTCCATCGGTATTACTCACGTACATCGGACAAGCAGCTTTCCTGAGTAAGAACCCGAAGAATGTTGCCAATACCTTCTTTGCCGCTACTCCAATTTCTTTGTTCTGGCCTACGTTCATCATGGCGATAGCTGCATCCATAATAGGAAGCCAAGCAATGATATCTTGTGCCTTTGCAACTGTTTCTCATTTACAATCACTTAGTTGCTTCCCAAGGGTCAAGATATTGCACACATCAAAGCGATTTCCGGGCCAATTGTACATCCCTGGAGTAAACTTCTTGTTATGTGTGGCTGCTTGCGTTGTGACTGTTAGCTTCAAAACAACTGTTATTATAGGAAAGGCGCACGAAATTTGTGTCATCCTTGTGATGATCATCACAACATTACTAATGACAATAGTTATGCTCTTGGTATGGAAGATCAACATCTTGTGGGTTGCTCTGTTTTTTATCACATTCACATCAACGGAGGCTGTCTACCTATCGTCAGTGCTATACAAGTTCACACATGGACCATATGTGCCTGTGGCTATGTCGGTAGTTCTAATGGTGGTGATGATCGTGTGGCACTACGTGCATGTGAAAAGATACAAGTATGAATTGGAACACACAGTATCCACCGACAAGGTGAAAGAAATGCTGGAGAGCCATGATCTGAAGAGGGTCCGTGGCGTAGCCCTGTTCTACACTGAACTAGTCCAAGGCATCCCGCCCATATTCCCGCACCTCATCGAGAAGATCCCAACGATTCATTCTGTTCTTGTCTTCATCTCTATCAAGCATTTGCCAGTACCCCATGTTGACACCTCGGAGCGATTCCTCTTCCGTCAAGTAGAGCTAAAGGACTACAAAGTGTTTCGATGTGTGGCACGATATGGATATCGTGATTCCCTCGAGGAGGCCAAGGACTTTGTTGTTACCCTTCTTGAGAACCTTCAGGATTATATAAGGGATGTTAATCTCTACACCGATGAGCCACACACCATTAGTGCCCATAGTTCTTGCAACCATAGCTTTTCTCGGGAGAAGCCTTCTGGACGGTACGCGGTACATGCCGAGGATATGCTTACACCAATTGAATCCTTCTCTGAGATCACAGCTCTATCTAACTATGGAAGCGACCGTCTACCACACTTCAAGGCAAGTAAGATGAATATGGAGGAGTTGGCGAAGATTGAGCAAGAGCAGATGTTCATTGAGAAGGAGATGGAAAAAGGTGTTGTATACATACTTGGAGAAACCGAGGTGGTTGTAAGGCCTCACTCATCGCTTCTTAAGAAGATAGTAGTGAACTACGTCTACTCTTTCTTAAGAAAGAACTTCGTGCAAGGGCAGAAGATGCTATTTATTCCCCATAGGCAATTGCTTAAAGTAGGGATCTCATATGAGATTTAG
Protein Sequence
  • >LOC_Os03g37930.1
    MDPGVEKKKQQMELVDVESGGLPVERQDSLFREAVRAEHAGAAHWDEQDSWGRTMSLAFQCVGILYGDIGTSSLYVYSSTFEHGIGHPDDVVGVLSLIVYSFMLFTVIKIVFVALHANDHGDGGTFALYSLISRHAKVSLIPNHQAEDELISGYSSSGKPSATLRRAHWLKQLLEASKAAKISLFLLTILAIAMVISDAVLTPPISVLSAVGGLREKVPHLTTDQIVWITVAILVVLFAIQRYGTDKVGYSFAPIILLWLLLIGATGLYNLIKHDISVLRAFNPKYIIDYFRRNKKEGWVSLGSILLCFTGSEALFANLGYFSIRSIQLSFSFALLPSVLLTYIGQAAFLSKNPKNVANTFFAATPISLFWPTFIMAIAASIIGSQAMISCAFATVSHLQSLSCFPRVKILHTSKRFPGQLYIPGVNFLLCVAACVVTVSFKTTVIIGKAHEICVILVMIITTLLMTIVMLLVWKINILWVALFFITFTSTEAVYLSSVLYKFTHGPYVPVAMSVVLMVVMIVWHYVHVKRYKYELEHTVSTDKVKEMLESHDLKRVRGVALFYTELVQGIPPIFPHLIEKIPTIHSVLVFISIKHLPVPHVDTSERFLFRQVELKDYKVFRCVARYGYRDSLEEAKDFVVTLLENLQDYIRDVNLYTDEPHTISAHSSCNHSFSREKPSGRYAVHAEDMLTPIESFSEITALSNYGSDRLPHFKASKMNMEELAKIEQEQMFIEKEMEKGVVYILGETEVVVRPHSSLLKKIVVNYVYSFLRKNFVQGQKMLFIPHRQLLKVGISYEI*