Information report for OsIRO3;OsOsbHLH063
Gene Details
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Functional Descriptions
- RESULTS: Transcript abundance of one nuclear located basic helix-loop-helix family transcription factor, OsIRO3, is up-regulated from 25- to 90-fold under Fe deficiency in both root and shoot respectively.
- CONCLUSION: A novel Fe regulated bHLH transcription factor (OsIRO3) that plays an important role for Fe homeostasis in rice was identified.
- Furthermore, the Fe concentration in shoots of transgenic rice plants over-expressing OsIRO3 was less than that in wild-type plants.
- OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage.
- Knockout of OsIRO3 resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development.
- OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice.
- However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear.
- Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice.
- Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the OsNAS3 expression.
- A Model to Incorporate the bHLH Transcription Factor OsIRO3 within the Rice Iron Homeostasis Regulatory Network.
- We place our results in the context of the existing literature and generate a model describing the role of OsIRO3 in rice Fe homeostasis and reinforce the essential function of OsIRO3 in the rice Fe deficiency response.
- Further analysis revealed that OsIRO3 directly regulated the expression of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2 (OsIRO2), which encodes a positive regulator of the Fe uptake system.
- Loss-of-function of OsIRO3 caused upregulation of Fe deficiency-associated genes in the root.
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2.
- Further investigation demonstrated that OsIRO3 interacted with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1(OsPRI1) and OsPRI2, and.
- This work sheds light on the molecular mechanism by which OsIRO3 modulates Fe homeostasis in rice.
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2.
Functional Keywords
- root , transcription-factor , homeostasis , shoot , leaf , growth , development , vegetative , root-development , iron , Fe , iron-homeostasis , Fe-homeostasis
Literature and News
- Identification of a novel iron regulated basic helix-loop-helix protein involved in Fe homeostasis in Oryza sativa . DOI: 10.1186/1471-2229-10-166 ; PMID: 20699001
- OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice . DOI: 10.3390/plants9091095 ; PMID: 32854449
- A Model to Incorporate the bHLH Transcription Factor OsIRO3 within the Rice Iron Homeostasis Regulatory Network . DOI: 10.3390/ijms23031635 ; PMID: 35163555
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2 . DOI: 10.1111/tpj.15864 ; PMID: 35689518
Gene Resources
- UniProt: Q10KL8
- EMBL: AC084762, AK061515, AK068704
- AlphaFoldDB: Q10KL8
- EnsemblPlants: Os03t0379300-01, Os03t0379300-02
- Gramene: Os03t0379300-01, Os03t0379300-02
- KEGG: dosa:Os03g0379300
- Orthologous matrix: KELTMPH
- InterPro: IPR011598, IPR036638
- PANTHER: PTHR47075, PTHR47075:SF9
- SUPFAM: SSF47459
- PROSITE: PS50888
- Gene3D: 4.10.280.10
- OrthoDB: Q10KL8
- SWISS-MODEL: Q10KL8
- eggNOG: ENOG502RYQ6
Sequences
cDNA Sequence
- >LOC_Os03g26210.3
TGTTATTGCTCCCTTCGCGGCTCTCCTTCTTCTTCTTCAATCTTCGCCCTCCTCCTCCTCCTCGTACGCGCCCACGCGGTTCTCTTTCTGCCCCCTCGATCGATTTGAGGGAACGGAAGGGGGAGGTTTTTGGAGGAGGCCATGGTGCCGTCGGAGAGGGGGGACGTCGCCACCGCCATCAGACCCGCCGCTGCCGACAAGCTCGTCCATGGGCCTATCTCTGACAAGAAGTGCCGGAAGAAGGTCCCGAGGAAGGTTCACAAATCTGAGAGGGAGAAGCTTAAACGCGGGCATCTCAATGATCTGTTCGGCGAGCTGGGTAATATGCTAGAAGCGGATAGGCAGAGTAATGGGAAGGCATGCATACTAACTGACACCACCCGGATCTTAAGAGATCTGCTTTCTCAAGTAAAATCTCTCCGGCAAGAAAACAGCACCCTCCAGAATGAATCTAATTATGTTACGATGGAAAGGAATGAGTTGCAGGATGAAAATGGTGCGCTACGCAGCGAAATCTCTGACCTCCAAAACGAGCTGAGGATGCGTGCGACAGGGAGTCCAGGTTGGGGCCATGGCGCGACTGGATCACCTCTCCCCGTTCCTCCCTCTCCAGGCACGGTTTTCCCATCGCAGCAGCCAATGCAGCCATCTCCCATGACAACAAGCACAGTGTTCCCCTTGCAGCAGCCACTGCCACAACCAACAGTCATTGAGCCCTCTGCAAGACAGCCACTGGAACTGAAGCTCTTCCTAGAAGCACCCCCCGCCGAAGACCCTGAACCATCAGAAGACCAGGAGGCTCCGAACAATGTGGCACGACCGCAGCCGAGGTACCCAACAGAGGCATCTTCTTGGCCTATTAGCCTGGGACTTCCAAGAATGGAAGATGAGCAAATGTAGTGGTAGCACGATAGATGATAGCACAGGTAGAGATTGAGAGGGTCTGTTTTTAATGCATGCAGGAATTCCACACGAAAACACTTCTGTCCTTGCATGAAAACTGGAAAATTTCCTGCATTCCAAGTAAGACCTGAGTAGATCAGCTCCAACCAATCAGAAACTCACTTTGAGCCTAGAGGATATACTACTGAATGATGTGCATGTAAATATTTCAATCTCCCTTTTGTTTCTGTAAACTCTAGTGAGAATAGAACCCGTTTTATCCCCTTGCAAATATTGATGTCCAATTAAAATGCATTTATGCGATAATTGCAATCGAAGGAAAAGTTATGAAAG - >LOC_Os03g26210.1
TGTTATTGCTCCCTTCGCGGCTCTCCTTCTTCTTCTTCAATCTTCGCCCTCCTCCTCCTCCTCGTACGCGCCCACGCGGTTCTCTTTCTGCCCCCTCGATCGATTTGAGGGAACGGAAGGGGGAGGTTTTTGGAGGAGGCCATGGTGCCGTCGGAGAGGGGGGACGTCGCCACCGCCATCAGACCCGCCGCTGCCGACAAGCTCGTCCATGGGCCTATCTCTGACAAGAAGTGCCGGAAGAAGGTCCCGAGGAAGGTTCACAAATCTGAGAGGGAGAAGCTTAAACGCGGGCATCTCAATGATCTGTTCGGCGAGCTGGGTAATATGCTAGAAGCGGATAGGCAGAGTAATGGGAAGGCATGCATACTAACTGACACCACCCGGATCTTAAGAGATCTGCTTTCTCAAGTAAAATCTCTCCGGCAAGAAAACAGCACCCTCCAGAATGAATCTAATTATGTTACGATGGAAAGGAATGAGTTGCAGGATGAAAATGGTGCGCTACGCAGCGAAATCTCTGACCTCCAAAACGAGCTGAGGATGCGTGCGACAGGGAGTCCAGGTTGGGGCCATGGCGCGACTGGATCACCTCTCCCCGTTCCTCCCTCTCCAGGCACGGTTTTCCCATCGCAGCAGCCAATGCAGCCATCTCCCATGACAACAAGCACAGTGTTCCCCTTGCAGCAGCCACTGCCACAACCAACAGTCATTGAGCCCTCTGCAAGACAGCCACTGGAACTGAAGCTCTTCCTAGAAGCACCCCCCGCCGAAGACCCTGAACCATCAGAAGACCAGGAGGCTCCGAACAATGTGGCACGACCGCAGCCGAGGTACCCAACAGAGGCATCTTCTTGGCCTATTAGCCTGGGACTTCCAAGAATGGAAGATGAGCAAATGTAGTGGTAGCACGATAGATGATAGCACAGGTAGAGATTGAGAGGGTCTGTTTTTAATGCATGCAGGAATTCCACACGAAAACACTTCTGTCCTTGCATGAAAACTGGAAAATTTCCTGCATTCCAAGTAAGACCTGAGTAGATCAGCTCCAACCAATCAGAAACTCACTTTGAGCCTAGAGGATATACTACTGAATGATGTGCATGTAAATATTTCAATCTCCCTTTTGTTTCTGTAAACTCTAGTGAGAATAGAACCCGTTTTATCCCCTTGCAAATATTGATGTCCAATTAAAATGCATTTATGCGATAATTGCAATCGAAGGAAAAGTTATGAAAG - >LOC_Os03g26210.2
TGTTATTGCTCCCTTCGCGGCTCTCCTTCTTCTTCTTCAATCTTCGCCCTCCTCCTCCTCCTCGTACGCGCCCACGCGGTTCTCTTTCTGCCCCCTCGATCGATTTGAGGGAACGGAAGGGGGAGGTTTTTGGAGGAGGCCATGGTGCCGTCGGAGAGGGGGGACGTCGCCACCGCCATCAGACCCGCCGCTGCCGACAAGCTCGTCCATGGGCCTATCTCTGACAAGAAGTGCCGGAAGAAGGTCCCGAGGAAGGTTCACAAATCTGAGAGGGAGAAGCTTAAACGCGGGCATCTCAATGATCTGTTCGGCGAGCTGGGTAATATGCTAGAAGCGGATAGGCAGAGTAATGGGAAGGCATGCATACTAACTGACACCACCCGGATCTTAAGAGATCTGCTTTCTCAAGTAAAATCTCTCCGGCAAGAAAACAGCACCCTCCAGAATGAATCTAATTATGTTACGATGGAAAGGAATGAGTTGCAGGATGAAAATGGTGCGCTACGCAGCGAAATCTCTGACCTCCAAAACGAGCTGAGGATGCGTGCGACAGGGAGTCCAGGTTGGGGCCATGGCGCGACTGGATCACCTCTCCCCGTTCCTCCCTCTCCAGGCACGGTTTTCCCATCGCAGCAGCCAATGCAGCCATCTCCCATGACAACAAGCACAGTGTTCCCCTTGCAGCAGCCACTGCCACAACCAACAGTCATTGAGCCCTCTGCAAGACAGCCACTGGAACTGAAGCTCTTCCTAGAAGCACCCCCCGCCGAAGACCCTGAACCATCAGAAGACCAGGAGGCTCCGAACAATGTGGCACGACCGCAGCCGAGGTACCCAACAGAGGCATCTTCTTGGCCTATTAGCCTGGGACTTCCAAGAATGGAAGATGAGCAAATGTAGTGGTAGCACGATAGATGATAGCACAGGTAGAGATTGAGAGGGTCTGTTTTTAATGCATGCAGGAATTCCACACGAAAACACTTCTGTCCTTGCATGAAAACTGGAAAATTTCCTGCATTCCAAGTAAGACCTGAGTAGATCAGCTCCAACCAATCAGAAACTCACTTTGAGCCTAGAGGATATACTACTGAATGATGTGCATGTAAATATTTCAATCTCCCTTTTGTTTCTGTAAACTCTAGTGAGAATAGAACCCGTTTTATCCCCTTGCAAATATTGATGTCCAATTAAAATGCATTTATGCGATAATTGCAATCGAAGGAAAAGTTATGAAAG
CDS Sequence
- >LOC_Os03g26210.3
ATGGTGCCGTCGGAGAGGGGGGACGTCGCCACCGCCATCAGACCCGCCGCTGCCGACAAGCTCGTCCATGGGCCTATCTCTGACAAGAAGTGCCGGAAGAAGGTCCCGAGGAAGGTTCACAAATCTGAGAGGGAGAAGCTTAAACGCGGGCATCTCAATGATCTGTTCGGCGAGCTGGGTAATATGCTAGAAGCGGATAGGCAGAGTAATGGGAAGGCATGCATACTAACTGACACCACCCGGATCTTAAGAGATCTGCTTTCTCAAGTAAAATCTCTCCGGCAAGAAAACAGCACCCTCCAGAATGAATCTAATTATGTTACGATGGAAAGGAATGAGTTGCAGGATGAAAATGGTGCGCTACGCAGCGAAATCTCTGACCTCCAAAACGAGCTGAGGATGCGTGCGACAGGGAGTCCAGGTTGGGGCCATGGCGCGACTGGATCACCTCTCCCCGTTCCTCCCTCTCCAGGCACGGTTTTCCCATCGCAGCAGCCAATGCAGCCATCTCCCATGACAACAAGCACAGTGTTCCCCTTGCAGCAGCCACTGCCACAACCAACAGTCATTGAGCCCTCTGCAAGACAGCCACTGGAACTGAAGCTCTTCCTAGAAGCACCCCCCGCCGAAGACCCTGAACCATCAGAAGACCAGGAGGCTCCGAACAATGTGGCACGACCGCAGCCGAGGTACCCAACAGAGGCATCTTCTTGGCCTATTAGCCTGGGACTTCCAAGAATGGAAGATGAGCAAATGTAG - >LOC_Os03g26210.1
ATGGTGCCGTCGGAGAGGGGGGACGTCGCCACCGCCATCAGACCCGCCGCTGCCGACAAGCTCGTCCATGGGCCTATCTCTGACAAGAAGTGCCGGAAGAAGGTCCCGAGGAAGGTTCACAAATCTGAGAGGGAGAAGCTTAAACGCGGGCATCTCAATGATCTGTTCGGCGAGCTGGGTAATATGCTAGAAGCGGATAGGCAGAGTAATGGGAAGGCATGCATACTAACTGACACCACCCGGATCTTAAGAGATCTGCTTTCTCAAGTAAAATCTCTCCGGCAAGAAAACAGCACCCTCCAGAATGAATCTAATTATGTTACGATGGAAAGGAATGAGTTGCAGGATGAAAATGGTGCGCTACGCAGCGAAATCTCTGACCTCCAAAACGAGCTGAGGATGCGTGCGACAGGGAGTCCAGGTTGGGGCCATGGCGCGACTGGATCACCTCTCCCCGTTCCTCCCTCTCCAGGCACGGTTTTCCCATCGCAGCAGCCAATGCAGCCATCTCCCATGACAACAAGCACAGTGTTCCCCTTGCAGCAGCCACTGCCACAACCAACAGTCATTGAGCCCTCTGCAAGACAGCCACTGGAACTGAAGCTCTTCCTAGAAGCACCCCCCGCCGAAGACCCTGAACCATCAGAAGACCAGGAGGCTCCGAACAATGTGGCACGACCGCAGCCGAGGTACCCAACAGAGGCATCTTCTTGGCCTATTAGCCTGGGACTTCCAAGAATGGAAGATGAGCAAATGTAG - >LOC_Os03g26210.2
ATGGTGCCGTCGGAGAGGGGGGACGTCGCCACCGCCATCAGACCCGCCGCTGCCGACAAGCTCGTCCATGGGCCTATCTCTGACAAGAAGTGCCGGAAGAAGGTCCCGAGGAAGGTTCACAAATCTGAGAGGGAGAAGCTTAAACGCGGGCATCTCAATGATCTGTTCGGCGAGCTGGGTAATATGCTAGAAGCGGATAGGCAGAGTAATGGGAAGGCATGCATACTAACTGACACCACCCGGATCTTAAGAGATCTGCTTTCTCAAGTAAAATCTCTCCGGCAAGAAAACAGCACCCTCCAGAATGAATCTAATTATGTTACGATGGAAAGGAATGAGTTGCAGGATGAAAATGGTGCGCTACGCAGCGAAATCTCTGACCTCCAAAACGAGCTGAGGATGCGTGCGACAGGGAGTCCAGGTTGGGGCCATGGCGCGACTGGATCACCTCTCCCCGTTCCTCCCTCTCCAGGCACGGTTTTCCCATCGCAGCAGCCAATGCAGCCATCTCCCATGACAACAAGCACAGTGTTCCCCTTGCAGCAGCCACTGCCACAACCAACAGTCATTGAGCCCTCTGCAAGACAGCCACTGGAACTGAAGCTCTTCCTAGAAGCACCCCCCGCCGAAGACCCTGAACCATCAGAAGACCAGGAGGCTCCGAACAATGTGGCACGACCGCAGCCGAGGTACCCAACAGAGGCATCTTCTTGGCCTATTAGCCTGGGACTTCCAAGAATGGAAGATGAGCAAATGTAG
Protein Sequence
- >LOC_Os03g26210.3
MVPSERGDVATAIRPAAADKLVHGPISDKKCRKKVPRKVHKSEREKLKRGHLNDLFGELGNMLEADRQSNGKACILTDTTRILRDLLSQVKSLRQENSTLQNESNYVTMERNELQDENGALRSEISDLQNELRMRATGSPGWGHGATGSPLPVPPSPGTVFPSQQPMQPSPMTTSTVFPLQQPLPQPTVIEPSARQPLELKLFLEAPPAEDPEPSEDQEAPNNVARPQPRYPTEASSWPISLGLPRMEDEQM* - >LOC_Os03g26210.1
MVPSERGDVATAIRPAAADKLVHGPISDKKCRKKVPRKVHKSEREKLKRGHLNDLFGELGNMLEADRQSNGKACILTDTTRILRDLLSQVKSLRQENSTLQNESNYVTMERNELQDENGALRSEISDLQNELRMRATGSPGWGHGATGSPLPVPPSPGTVFPSQQPMQPSPMTTSTVFPLQQPLPQPTVIEPSARQPLELKLFLEAPPAEDPEPSEDQEAPNNVARPQPRYPTEASSWPISLGLPRMEDEQM* - >LOC_Os03g26210.2
MVPSERGDVATAIRPAAADKLVHGPISDKKCRKKVPRKVHKSEREKLKRGHLNDLFGELGNMLEADRQSNGKACILTDTTRILRDLLSQVKSLRQENSTLQNESNYVTMERNELQDENGALRSEISDLQNELRMRATGSPGWGHGATGSPLPVPPSPGTVFPSQQPMQPSPMTTSTVFPLQQPLPQPTVIEPSARQPLELKLFLEAPPAEDPEPSEDQEAPNNVARPQPRYPTEASSWPISLGLPRMEDEQM*