Information report for OsBBS1;OsRLCK109
Gene Details
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Functional Descriptions
- A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.).
- Transgenic LOC_Os03g24930 knockout plants generated by a CRISPR/Cas9 strategy exhibited similar early leaf senescence phenotypes as did the bbs1 mutant, which confirmed that LOC_Os03g24930 was the OsBBS1 gene.
- The OsBBS1 gene was cloned by a map-based cloning strategy, and a guanine (G) insertion was found in the first exon of LOC_Os03g24930.
- The expression of OsBBS1 could be greatly induced by salt stress, and the bbs1 mutant exhibited hypersensitivity to salt stress.
- Chloroplast degradation was observed in LMM24 leaves, with decreased expression of photosynthesis-related genes and increased expression of the senescence-induced STAYGREEN (SGR) gene and other senescence-associated genes.
- The LMM24 mutant displayed dark brown lesions in leaves and growth retardation that were not observed in wild-type ZH8015.
- Furthermore, LMM24 exhibited enhanced resistance to rice blast fungus Magnaporthe oryzae (M.
- LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice.
- Our data demonstrate that LMM24 regulates cell death and defense responses in rice.
- The results of histochemical staining and TUNEL assays showed enhanced ROS accumulation and cell death in LMM24.
- The LMM24 gene was identified by MutMap, and LMM24 was confirmed as a receptor-like cytoplasmic kinase 109 by amino acid sequence analysis.
- We identified a new rice lesion mimic mutant LMM24 from a mutant pool of indica rice cultivar "ZhongHui8015".
Functional Keywords
- leaf , leaf-senescence , early-leaf-senescence , senescence , map-based-cloning , salt , salt-stress , stress , chloroplast , growth , resistance , defense , defense-response , blast , magnaporthe-oryzae , cell-death , Kinase , lesion , lesion-mimic
Literature and News
- A guanine insert in OsBBS1 leads to early leaf senescence and salt stress sensitivity in rice (Oryza sativa L.) . DOI: 10.1007/s00299-018-2280-y ; PMID: 29572657
- LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice . DOI: 10.3390/ijms20133243 ; PMID: 31269643
Gene Resources
- UniProt: Q10KZ7
- EMBL: AK066529, AP014959, DP000009
- AlphaFoldDB: Q10KZ7
- EnsemblPlants: Os03t0364400-01, Os03t0364400-02
- Gramene: Os03t0364400-01, Os03t0364400-02
- KEGG: dosa:Os03g0364400, osa:4332906
- Orthologous matrix: KPFTANC
- InterPro: IPR000719, IPR001245, IPR008271
Homologs
- Saccharum spontaneum: Sspon.01G0039560-1B
- Setaria italica: Seita.9G394900
- Setaria viridis: SEVIR_9G397400v2
- Triticum aestivum: Traes_4DL_6D841BCF3
- Zea mays: Zm00001d029258
Sequences
cDNA Sequence
- >LOC_Os03g24930.1
GGCTCGGATCGGAGCAGTTGGCTGCAGCCGCAGTTTGTTGGAGCGGTTGGCCCTCGCACTCGCACTACCCCCCGCCCTTTTTCTCCCGCCATATTTCTACTTCTCCCGGCCCACCCCCCCTCAGCTCGCCCTCCTCCCGCCCCCCACCTGCTCGATCTCCACTCCGTCTCCATCTCCATCCGATCCAAGATGCCGCCGAGGCAGTGGAGGCCGATGCTGGCGTCGGCGACCAAGTGCTGCGCGGCGGAGGACGCGGTGGTGGCGGTGGATGGCAGCGGCGGCGGGGGCGGCCTCGCGCGGTGCAGGCCGGCGCGGTCCGAGTTCTCACGGAGGCTGGCGTCGTTCCGGAAGCTGTCGTCGATGACCAACAGCCCGGCGTCGTCCGTCGCGGGGGCGGCGGAGGGGGGGAAAGACGACGGCGAGGAGGGGGGCGGCGGCGGTGGCGGCGTGTCCGGCCCGCTGCAGCTCTACTCCTTCAGCTTCTCCGAGCTCCGGTCCATCACGCACGACTTCTCCAGCAGCTACCTGCTCGGGGAGGGCGGCTTCGGCGCCGTCCACAAGGGCTTCGTCGACGCCGGCATGCGCCCCGGCCTCCTGCCCCAGCCCGTCGCCGTCAAGCAGCTCGACATCGCCGGCCTCCAGGGCCACCGGGAGTGGCTGGCGGAGGTGATCTTCTTGGGGCAGTTCCGGCACCCGCACCTGGTGAAGCTGCTGGGCTACTGCTGCGAGGACGAGGAGCGCCTCCTCGTCTACGAGTTCATGCCGCGCGGCAGCCTCGAGAACCACCTCTTCAAGAGGATATCGGCGACGGTGCCGTGGGGCACGCGTCTGAAGATCGCCATCGGCGCCGCCAAGGGCCTCGCCTTCCTCCACGGCGCCTCCACCCCCGTCATCTACCGCGACTTCAAGGCCTCCAACATCCTCCTCGACTCGGAGTTCACGGCGAAGCTGTCGGACTTCGGGCTGGCGAAGATGGGGCCGGAGGGTTCGGAGACTCACGTGACGACGCGGGTGATGGGCACGCACGGGTACGCGGCGCCGGAGTACGTGATGACGGGGCACCTCAACATCAAGAGCGACGTGTACAGCTACGGCGTCGTGCTCCTGGAGCTCCTGACGGGGCGGCGCGCCATGGAGCATGTCAGGGGGAGGAGCCTCCACGCCGACCAGGTGGTCAAGATCGTCGACTGGACCCGCCCCTACCTCGGCAGCAGCCGCCGCCTCCGCTGCATCATGGACCCGCGCCTCGCCGGCCACTACTCCGTCAAGGCCGCCCGCGCCGTCGCCCACCTCGCCGTCCAGTGCACCAGCCCGCAGCCGCGCGACCGCCCGCGCATGGCCGCCGTCGTCGACGCCCTCGAGCGCCTGCAGGGCTTCAAGGACATGGCCGTCACCGTCGGCCTCTGGCCCACCAACGCCCCCGTCGCCGGCAGGAACGCCATCTCCGCCAAGATCCGCGCCGAGGTCAGAGGCGCCGGCTCCGGCGGCGGCGCGGCCTCGCGGCGGCGGAGCGCCTCCGCCAAGCTGCCCTGATCGCCCGCGTGCGTTCGTGGGCGCGCCACGTCATCGCGAGTACGTATTTGTATACGTGTACGTGTGTGTATATACTTTAGCTGCTGTATAAGATGGCACGGCCGATGTATTGTACGTGGTGATCATCGAGCTTGATTGATTTTTTTTTAGCCATATGGATTATGGATTATTGCGGAATTAATACTGATCTGTATCACCATATGTACGTACTTAGTAGGAGTATTTAGTTTTAGGTGCCATGATGCTGGTAGATCATGCATGCATGTATGATGAATTGTACTTTCATCCACCGATTGCTGATTGCAAGTTCGTGCATCAGTAATCGGTCATTTCGATATTATCTTTTTTTTTCCTTGAAGA - >LOC_Os03g24930.2
GGCTCGGATCGGAGCAGTTGGCTGCAGCCGCAGTTTGTTGGAGCGGTTGGCCCTCGCACTCGCACTACCCCCCGCCCTTTTTCTCCCGCCATATTTCTACTTCTCCCGGCCCACCCCCCCTCAGCTCGCCCTCCTCCCGCCCCCCACCTGCTCGATCTCCACTCCGTCTCCATCTCCATCCGATCCAAGATGCCGCCGAGGCAGTGGAGGCCGATGCTGGCGTCGGCGACCAAGTGCTGCGCGGCGGAGGACGCGGTGGTGGCGGTGGATGGCAGCGGCGGCGGGGGCGGCCTCGCGCGGTGCAGGCCGGCGCGGTCCGAGTTCTCACGGAGGCTGGCGTCGTTCCGGAAGCTGTCGTCGATGACCAACAGCCCGGCGTCGTCCGTCGCGGGGGCGGCGGAGGGGGGGAAAGACGACGGCGAGGAGGGGGGCGGCGGCGGTGGCGGCGTGTCCGGCCCGCTGCAGCTCTACTCCTTCAGCTTCTCCGAGCTCCGGTCCATCACGCACGACTTCTCCAGCAGCTACCTGCTCGGGGAGGGCGGCTTCGGCGCCGTCCACAAGGGCTTCGTCGACGCCGGCATGCGCCCCGGCCTCCTGCCCCAGCCCGTCGCCGTCAAGCAGCTCGACATCGCCGGCCTCCAGGGCCACCGGGAGTGGCTGGCGGAGGTGATCTTCTTGGGGCAGTTCCGGCACCCGCACCTGGTGAAGCTGCTGGGCTACTGCTGCGAGGACGAGGAGCGCCTCCTCGTCTACGAGTTCATGCCGCGCGGCAGCCTCGAGAACCACCTCTTCAAGAGGATATCGGCGACGGTGCCGTGGGGCACGCGTCTGAAGATCGCCATCGGCGCCGCCAAGGGCCTCGCCTTCCTCCACGGCGCCTCCACCCCCGTCATCTACCGCGACTTCAAGGCCTCCAACATCCTCCTCGACTCGGAGTTCACGGCGAAGCTGTCGGACTTCGGGCTGGCGAAGATGGGGCCGGAGGGTTCGGAGACTCACGTGACGACGCGGGTGATGGGCACGCACGGGTACGCGGCGCCGGAGTACGTGATGACGGGGCACCTCAACATCAAGAGCGACGTGTACAGCTACGGCGTCGTGCTCCTGGAGCTCCTGACGGGGCGGCGCGCCATGGAGCATGTCAGGGGGAGGAGCCTCCACGCCGACCAGGTGGTCAAGATCGTCGACTGGACCCGCCCCTACCTCGGCAGCAGCCGCCGCCTCCGCTGCATCATGGACCCGCGCCTCGCCGGCCACTACTCCGTCAAGGCCGCCCGCGCCGTCGCCCACCTCGCCGTCCAGTGCACCAGCCCGCAGCCGCGCGACCGCCCGCGCATGGCCGCCGTCGTCGACGCCCTCGAGCGCCTGCAGGGCTTCAAGGACATGGCCGTCACCGTCGGCCTCTGGCCCACCAACGCCCCCGTCGCCGGCAGGAACGCCATCTCCGCCAAGATCCGCGCCGAGGTCAGAGGCGCCGGCTCCGGCGGCGGCGCGGCCTCGCGGCGGCGGAGCGCCTCCGCCAAGCTGCCCTGATCGCCCGCGTGCGTTCGTGGGCGCGCCACGTCATCGCGAGTACGTATTTGTATACGTGTACGTGTGTGTATATACTTTAGCTGCTGTATAAGATGGCACGGCCGATGTATTGTACGTGGTGATCATCGAGCTTGATTGATTTTTTTTTAGCCATATGGATTATGGATTATTGCGGAATTAATACTGATCTGTATCACCATATGTACGTACTTAGTAGGAGTATTTAGTTTTAGGTGCCATGATGCTGGTAGATCATGCATGCATGTATGATGAATTGTACTTTCATCCACCGATTGCTGATTGCAAGTTCGTGCATCAGTAATCGGTCATTTCGATATTATCTTTTTTTTTCCTTGAAGA
CDS Sequence
- >LOC_Os03g24930.1
ATGCCGCCGAGGCAGTGGAGGCCGATGCTGGCGTCGGCGACCAAGTGCTGCGCGGCGGAGGACGCGGTGGTGGCGGTGGATGGCAGCGGCGGCGGGGGCGGCCTCGCGCGGTGCAGGCCGGCGCGGTCCGAGTTCTCACGGAGGCTGGCGTCGTTCCGGAAGCTGTCGTCGATGACCAACAGCCCGGCGTCGTCCGTCGCGGGGGCGGCGGAGGGGGGGAAAGACGACGGCGAGGAGGGGGGCGGCGGCGGTGGCGGCGTGTCCGGCCCGCTGCAGCTCTACTCCTTCAGCTTCTCCGAGCTCCGGTCCATCACGCACGACTTCTCCAGCAGCTACCTGCTCGGGGAGGGCGGCTTCGGCGCCGTCCACAAGGGCTTCGTCGACGCCGGCATGCGCCCCGGCCTCCTGCCCCAGCCCGTCGCCGTCAAGCAGCTCGACATCGCCGGCCTCCAGGGCCACCGGGAGTGGCTGGCGGAGGTGATCTTCTTGGGGCAGTTCCGGCACCCGCACCTGGTGAAGCTGCTGGGCTACTGCTGCGAGGACGAGGAGCGCCTCCTCGTCTACGAGTTCATGCCGCGCGGCAGCCTCGAGAACCACCTCTTCAAGAGGATATCGGCGACGGTGCCGTGGGGCACGCGTCTGAAGATCGCCATCGGCGCCGCCAAGGGCCTCGCCTTCCTCCACGGCGCCTCCACCCCCGTCATCTACCGCGACTTCAAGGCCTCCAACATCCTCCTCGACTCGGAGTTCACGGCGAAGCTGTCGGACTTCGGGCTGGCGAAGATGGGGCCGGAGGGTTCGGAGACTCACGTGACGACGCGGGTGATGGGCACGCACGGGTACGCGGCGCCGGAGTACGTGATGACGGGGCACCTCAACATCAAGAGCGACGTGTACAGCTACGGCGTCGTGCTCCTGGAGCTCCTGACGGGGCGGCGCGCCATGGAGCATGTCAGGGGGAGGAGCCTCCACGCCGACCAGGTGGTCAAGATCGTCGACTGGACCCGCCCCTACCTCGGCAGCAGCCGCCGCCTCCGCTGCATCATGGACCCGCGCCTCGCCGGCCACTACTCCGTCAAGGCCGCCCGCGCCGTCGCCCACCTCGCCGTCCAGTGCACCAGCCCGCAGCCGCGCGACCGCCCGCGCATGGCCGCCGTCGTCGACGCCCTCGAGCGCCTGCAGGGCTTCAAGGACATGGCCGTCACCGTCGGCCTCTGGCCCACCAACGCCCCCGTCGCCGGCAGGAACGCCATCTCCGCCAAGATCCGCGCCGAGGTCAGAGGCGCCGGCTCCGGCGGCGGCGCGGCCTCGCGGCGGCGGAGCGCCTCCGCCAAGCTGCCCTGA - >LOC_Os03g24930.2
ATGCCGCCGAGGCAGTGGAGGCCGATGCTGGCGTCGGCGACCAAGTGCTGCGCGGCGGAGGACGCGGTGGTGGCGGTGGATGGCAGCGGCGGCGGGGGCGGCCTCGCGCGGTGCAGGCCGGCGCGGTCCGAGTTCTCACGGAGGCTGGCGTCGTTCCGGAAGCTGTCGTCGATGACCAACAGCCCGGCGTCGTCCGTCGCGGGGGCGGCGGAGGGGGGGAAAGACGACGGCGAGGAGGGGGGCGGCGGCGGTGGCGGCGTGTCCGGCCCGCTGCAGCTCTACTCCTTCAGCTTCTCCGAGCTCCGGTCCATCACGCACGACTTCTCCAGCAGCTACCTGCTCGGGGAGGGCGGCTTCGGCGCCGTCCACAAGGGCTTCGTCGACGCCGGCATGCGCCCCGGCCTCCTGCCCCAGCCCGTCGCCGTCAAGCAGCTCGACATCGCCGGCCTCCAGGGCCACCGGGAGTGGCTGGCGGAGGTGATCTTCTTGGGGCAGTTCCGGCACCCGCACCTGGTGAAGCTGCTGGGCTACTGCTGCGAGGACGAGGAGCGCCTCCTCGTCTACGAGTTCATGCCGCGCGGCAGCCTCGAGAACCACCTCTTCAAGAGGATATCGGCGACGGTGCCGTGGGGCACGCGTCTGAAGATCGCCATCGGCGCCGCCAAGGGCCTCGCCTTCCTCCACGGCGCCTCCACCCCCGTCATCTACCGCGACTTCAAGGCCTCCAACATCCTCCTCGACTCGGAGTTCACGGCGAAGCTGTCGGACTTCGGGCTGGCGAAGATGGGGCCGGAGGGTTCGGAGACTCACGTGACGACGCGGGTGATGGGCACGCACGGGTACGCGGCGCCGGAGTACGTGATGACGGGGCACCTCAACATCAAGAGCGACGTGTACAGCTACGGCGTCGTGCTCCTGGAGCTCCTGACGGGGCGGCGCGCCATGGAGCATGTCAGGGGGAGGAGCCTCCACGCCGACCAGGTGGTCAAGATCGTCGACTGGACCCGCCCCTACCTCGGCAGCAGCCGCCGCCTCCGCTGCATCATGGACCCGCGCCTCGCCGGCCACTACTCCGTCAAGGCCGCCCGCGCCGTCGCCCACCTCGCCGTCCAGTGCACCAGCCCGCAGCCGCGCGACCGCCCGCGCATGGCCGCCGTCGTCGACGCCCTCGAGCGCCTGCAGGGCTTCAAGGACATGGCCGTCACCGTCGGCCTCTGGCCCACCAACGCCCCCGTCGCCGGCAGGAACGCCATCTCCGCCAAGATCCGCGCCGAGGTCAGAGGCGCCGGCTCCGGCGGCGGCGCGGCCTCGCGGCGGCGGAGCGCCTCCGCCAAGCTGCCCTGA
Protein Sequence
- >LOC_Os03g24930.1
MPPRQWRPMLASATKCCAAEDAVVAVDGSGGGGGLARCRPARSEFSRRLASFRKLSSMTNSPASSVAGAAEGGKDDGEEGGGGGGGVSGPLQLYSFSFSELRSITHDFSSSYLLGEGGFGAVHKGFVDAGMRPGLLPQPVAVKQLDIAGLQGHREWLAEVIFLGQFRHPHLVKLLGYCCEDEERLLVYEFMPRGSLENHLFKRISATVPWGTRLKIAIGAAKGLAFLHGASTPVIYRDFKASNILLDSEFTAKLSDFGLAKMGPEGSETHVTTRVMGTHGYAAPEYVMTGHLNIKSDVYSYGVVLLELLTGRRAMEHVRGRSLHADQVVKIVDWTRPYLGSSRRLRCIMDPRLAGHYSVKAARAVAHLAVQCTSPQPRDRPRMAAVVDALERLQGFKDMAVTVGLWPTNAPVAGRNAISAKIRAEVRGAGSGGGAASRRRSASAKLP* - >LOC_Os03g24930.2
MPPRQWRPMLASATKCCAAEDAVVAVDGSGGGGGLARCRPARSEFSRRLASFRKLSSMTNSPASSVAGAAEGGKDDGEEGGGGGGGVSGPLQLYSFSFSELRSITHDFSSSYLLGEGGFGAVHKGFVDAGMRPGLLPQPVAVKQLDIAGLQGHREWLAEVIFLGQFRHPHLVKLLGYCCEDEERLLVYEFMPRGSLENHLFKRISATVPWGTRLKIAIGAAKGLAFLHGASTPVIYRDFKASNILLDSEFTAKLSDFGLAKMGPEGSETHVTTRVMGTHGYAAPEYVMTGHLNIKSDVYSYGVVLLELLTGRRAMEHVRGRSLHADQVVKIVDWTRPYLGSSRRLRCIMDPRLAGHYSVKAARAVAHLAVQCTSPQPRDRPRMAAVVDALERLQGFKDMAVTVGLWPTNAPVAGRNAISAKIRAEVRGAGSGGGAASRRRSASAKLP*