Gene Details:

Functional Descriptions:

  • Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity.
  • OsSPK1 contributes to signaling by two disease-resistance genes, Pit and Pia, against the rice blast fungus Magnaporthe oryzae and facilitates OsRac1 activation in vitro and in vivo.
  • Overall, we conclude that OsSPK1 is a direct and key signaling target of Pit-mediated immunity.
  • OsSPK1 activates the small GTPase OsRac1, which in turn transduces the signal to the transcription factor RAC IMMUNITY1 (RAI1).
  • We found that in blast resistance mediated by the NLR PIRICULARIA ORYZAE RESISTANCE IN DIGU 3 (PID3), the guanine nucleotide exchange factor OsSPK1 works downstream of PID3.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g21080.1
    TTTCTTTTGTTATGATCTTCTATCAATTATTGAGCCACGCCAAGTTTTTGAGCTGGTTTCATTGTATATGGACAAGTTTGCAGGAGTTTGCCAGTCAATTCTCCATGATTGCAAATTGACATTCCTTCAGATAATATGTGACCATGATCTTTTTGTTGAGATGCCTGGTCGTGACCCCTCTGATAGAAATTATCTCTCGTCAGTACTTATTCAAGAGATTTTTCTCACCCTTGACCATGATGATCTATCTCAGCGAGCGAAAGCAGCCCGTATTTTGGTTGTTCTAATATGCAAGCATGAATTTGATGCACGATATCAAAAAAGTGAAGACAAGCTGTATATTGCTCAGCTGTATTTTTCTCTTATAGGACAGATTCTAGATGAGATGCCTGTATTCTATAATCTAAATGCTGTTGAAAAGCGTGAGGTGCTGGTAGTCATTTTGCAAATCATAAGGAACTTGGACGACATGACTCTGATCAAAGCATGGCAACAGAGCATTGCTAGAACCAGATTGTTCTTCAAGCTACTTGAAGAATGCATTACCCATTTTGAGCACAACAAAACAGGAGACAGCTTGTTACTAGGCTCAAGTTCACGGAGCCCTGATGCTGAGCGCCCTGCATCTCCAAAGTACTCCGATCGATTATCCCCATCAGTTAATGCATATTTGTCTGAGGCATCACGCCATGAAATAAGGCCTCAGGGAACACCAGAAAATGGATACATGTGGAACAGGGTTAGTCCTCAACTGAGCTCTCCTAACCAGCCCTATTCATTGAGAGAAGCACTTGCTCAAGCGCAGTCTTCAAGGATTGGATCAACCGCGAGGGCACTGAGAGAATCTCTACATCCAGTGCTGAGACAAAAGTTGGAACTGTGGGAAGAAAATCTCAGTACTGCTGTGAGCCTGGAAGTGTTGGGAATAATTGATAAGTTTTCAGTCGCTGCAGCTTCTCGTAGCATCACTACTGATTACGCAAAGCTAGATTGTGTAACATCTGTATTAATGGGTCTTTTATCAAGGAGCCAGCCCTTAGCTTTTTGGAAAGCTTTCCTTCCTGTGGTGTATAATATATTTAACCTCCATGGCGCAACACTGATGGCAAGGGAGAATGATCGCTTCTTGAAGCAAATTGCTTTTCATCTTTTGCGCCTTGCTGTTTTCCGGAATGATTCTATTAGAAAAAGGGCTGTTGTTGGGTTGCAGATTCTTGTTAGGAACTCGTTTAATTATTTTAAGAACACCACAAGGTTGAGGGTGATGTTGACAATCACTTTGTCAGAACTTATGTCTGATGTGCAAGTAACTCAGATGAAATCTGATGGCTCTCTTGAGGAAAGTGGTGAAACTCGGTGCCTTAGGAAATCACTAGAGGAAATGGCTGATGTACGAAGTAAGGATCTCTTGAAAGATTGTGGCCTCCCTGTTAATGCACTGGAGGCAGCGCCTGAAGGGTCGACTGATAATAGGTGGTCTTGGGTAGAAGTTAAACATCTATCCAAATGTCTAGTCCAGGCCCTTGATGCTGGTCTTGAACATGCCCTCTTGGGCTCTGAAATGACTCTGGACAGGTACGCTGCTGCTGAGGGTTTCTATAAGCTAGCAATGGCCTACGCGCCTGTGCCAGATCTTCATATAATGTGGTTACTGCATCTATGTGATGCACATCAGGAGATGCAATCATGGGCTGAAGCTGCACAATGTGCAGTTGCTGTAGCTGGTGTTATCATGCAGGCACTTGTTGGAAGGAATGATGCGGTGTGGAGTAAGGAGCATGTTGCTTCCCTATGTAAGATTTGCCCTATTGTGAACACTGATGTTAGCTCAGAAGCATCTGCAGCAGAAGTTGAGGGATATGGTGCATCCAAGCTTACTGTGGATTCAGCTGTCAAGTATCTTCAGCTGGCAAACAAACTTTTTGCACAAGCCGAACTCTATCACTTTTGTGCTAGTATTCAGGAACTCATCATTCCTGTGTATAAAAGCAGAAGGGCATATGGGCATCTGGCTAAGTGTCATACATCACTCAAGGATATCTATGAGTCAATTCTTGAACAGGAGGCTAGTCCTATACCATTCATTGATGCTACCTACTACCGGGTAGGGTTTTATGGGGAGCGGTTTGGCAAACTCAACAAAAAGGAGTATGTGTTCAGAGAGCCAAGAGATGTTCGTCTTGGTGACATAATGGAGAAGCTTAGTCATATCTATGAGGCTAAAATGGATGGCAATCACACCTTACACATCATTCCGGATTCGAGACAAGTCAACGCTGATGAGTTGCAACCTGGTGTCTGCTATCTGCAGATAACTGCTGTTGATCCTGTAATGGAGGATGAGGACCTAGGGAGTAGAAGGGAAAGGATTTTCTCTTTATCTACTGGTACTGTCCGGGCCCGTGTGTTCGACCGTTTTCTTTTTGATACACCATTCACAAAGAATGGAAAAACACAAGGTGGCCTTGAAGATCAGTGGAAGAGGCGCACAGTACTCCAGACAGAGGGTTCCTTTCCTGCTTTGGTGAATCGTCTGTTAGTGATCAAATCAGAATCTCTAGAGTTTTCTCCTGTTGAGAATGCGATTGGAATGATCGAGACAAGGACAGCTGCCTTGCGAAATGAACTAGAAGAACCACGCAGTTCGGAAGGTGACCAACTCCCAAGACTCCAAAGCCTGCAGAGGATACTCCAAGGAAGTGTGGCTGTTCAGGTAAATAGTGGTGTATTAAGTGTGTGCACTGCATTCTTATCTGGTGAGCCCGCAACTAGGCTCCGATCTCAAGAACTGCAGCAGCTCATCGCTGCATTGCTGGAATTCATGGCCGTCTGCAAGCGCGCAATCCGTGTGCATTTTAGATTGATAGGGGAAGAAGACCAGGAATTCCACACTCAACTAGTCAATGGCTTTCAGTCGCTCACAGCTGAGCTTTCACACTATATTCCTGCAATTCTTTCAGAGCTATAACTGTATTCTAGCAAGCTGGCAAGTAGGTAGCATTTGAATGGTTCATTCTTTCAGTTGGGGCTCCCCACCTTGTCCATAATTCCGTTAGTTTGGTTGTATAGTTCTGGAAAACTAACTAGGCTTTTCAATTCTGTTCTGTTTGCAATGCCGTGCCACGATTTTGGGGGTGTAAAGCTTGTGTAATCCTTAGTGTGTTGTATCTCTATTACCTTGTTTATGCGGTTTGTAGACTAATAGCAAGCTATATAGCAGTAACTATTCGAGTGGACAGTTGTGTTCATCTGGAGTGATATATAATGGCTATGGTTCTGGGTGTTCTTTCCTTCATAA
CDS Sequence
  • >LOC_Os03g21080.1
    ATGGACAAGTTTGCAGGAGTTTGCCAGTCAATTCTCCATGATTGCAAATTGACATTCCTTCAGATAATATGTGACCATGATCTTTTTGTTGAGATGCCTGGTCGTGACCCCTCTGATAGAAATTATCTCTCGTCAGTACTTATTCAAGAGATTTTTCTCACCCTTGACCATGATGATCTATCTCAGCGAGCGAAAGCAGCCCGTATTTTGGTTGTTCTAATATGCAAGCATGAATTTGATGCACGATATCAAAAAAGTGAAGACAAGCTGTATATTGCTCAGCTGTATTTTTCTCTTATAGGACAGATTCTAGATGAGATGCCTGTATTCTATAATCTAAATGCTGTTGAAAAGCGTGAGGTGCTGGTAGTCATTTTGCAAATCATAAGGAACTTGGACGACATGACTCTGATCAAAGCATGGCAACAGAGCATTGCTAGAACCAGATTGTTCTTCAAGCTACTTGAAGAATGCATTACCCATTTTGAGCACAACAAAACAGGAGACAGCTTGTTACTAGGCTCAAGTTCACGGAGCCCTGATGCTGAGCGCCCTGCATCTCCAAAGTACTCCGATCGATTATCCCCATCAGTTAATGCATATTTGTCTGAGGCATCACGCCATGAAATAAGGCCTCAGGGAACACCAGAAAATGGATACATGTGGAACAGGGTTAGTCCTCAACTGAGCTCTCCTAACCAGCCCTATTCATTGAGAGAAGCACTTGCTCAAGCGCAGTCTTCAAGGATTGGATCAACCGCGAGGGCACTGAGAGAATCTCTACATCCAGTGCTGAGACAAAAGTTGGAACTGTGGGAAGAAAATCTCAGTACTGCTGTGAGCCTGGAAGTGTTGGGAATAATTGATAAGTTTTCAGTCGCTGCAGCTTCTCGTAGCATCACTACTGATTACGCAAAGCTAGATTGTGTAACATCTGTATTAATGGGTCTTTTATCAAGGAGCCAGCCCTTAGCTTTTTGGAAAGCTTTCCTTCCTGTGGTGTATAATATATTTAACCTCCATGGCGCAACACTGATGGCAAGGGAGAATGATCGCTTCTTGAAGCAAATTGCTTTTCATCTTTTGCGCCTTGCTGTTTTCCGGAATGATTCTATTAGAAAAAGGGCTGTTGTTGGGTTGCAGATTCTTGTTAGGAACTCGTTTAATTATTTTAAGAACACCACAAGGTTGAGGGTGATGTTGACAATCACTTTGTCAGAACTTATGTCTGATGTGCAAGTAACTCAGATGAAATCTGATGGCTCTCTTGAGGAAAGTGGTGAAACTCGGTGCCTTAGGAAATCACTAGAGGAAATGGCTGATGTACGAAGTAAGGATCTCTTGAAAGATTGTGGCCTCCCTGTTAATGCACTGGAGGCAGCGCCTGAAGGGTCGACTGATAATAGGTGGTCTTGGGTAGAAGTTAAACATCTATCCAAATGTCTAGTCCAGGCCCTTGATGCTGGTCTTGAACATGCCCTCTTGGGCTCTGAAATGACTCTGGACAGGTACGCTGCTGCTGAGGGTTTCTATAAGCTAGCAATGGCCTACGCGCCTGTGCCAGATCTTCATATAATGTGGTTACTGCATCTATGTGATGCACATCAGGAGATGCAATCATGGGCTGAAGCTGCACAATGTGCAGTTGCTGTAGCTGGTGTTATCATGCAGGCACTTGTTGGAAGGAATGATGCGGTGTGGAGTAAGGAGCATGTTGCTTCCCTATGTAAGATTTGCCCTATTGTGAACACTGATGTTAGCTCAGAAGCATCTGCAGCAGAAGTTGAGGGATATGGTGCATCCAAGCTTACTGTGGATTCAGCTGTCAAGTATCTTCAGCTGGCAAACAAACTTTTTGCACAAGCCGAACTCTATCACTTTTGTGCTAGTATTCAGGAACTCATCATTCCTGTGTATAAAAGCAGAAGGGCATATGGGCATCTGGCTAAGTGTCATACATCACTCAAGGATATCTATGAGTCAATTCTTGAACAGGAGGCTAGTCCTATACCATTCATTGATGCTACCTACTACCGGGTAGGGTTTTATGGGGAGCGGTTTGGCAAACTCAACAAAAAGGAGTATGTGTTCAGAGAGCCAAGAGATGTTCGTCTTGGTGACATAATGGAGAAGCTTAGTCATATCTATGAGGCTAAAATGGATGGCAATCACACCTTACACATCATTCCGGATTCGAGACAAGTCAACGCTGATGAGTTGCAACCTGGTGTCTGCTATCTGCAGATAACTGCTGTTGATCCTGTAATGGAGGATGAGGACCTAGGGAGTAGAAGGGAAAGGATTTTCTCTTTATCTACTGGTACTGTCCGGGCCCGTGTGTTCGACCGTTTTCTTTTTGATACACCATTCACAAAGAATGGAAAAACACAAGGTGGCCTTGAAGATCAGTGGAAGAGGCGCACAGTACTCCAGACAGAGGGTTCCTTTCCTGCTTTGGTGAATCGTCTGTTAGTGATCAAATCAGAATCTCTAGAGTTTTCTCCTGTTGAGAATGCGATTGGAATGATCGAGACAAGGACAGCTGCCTTGCGAAATGAACTAGAAGAACCACGCAGTTCGGAAGGTGACCAACTCCCAAGACTCCAAAGCCTGCAGAGGATACTCCAAGGAAGTGTGGCTGTTCAGGTAAATAGTGGTGTATTAAGTGTGTGCACTGCATTCTTATCTGGTGAGCCCGCAACTAGGCTCCGATCTCAAGAACTGCAGCAGCTCATCGCTGCATTGCTGGAATTCATGGCCGTCTGCAAGCGCGCAATCCGTGTGCATTTTAGATTGATAGGGGAAGAAGACCAGGAATTCCACACTCAACTAGTCAATGGCTTTCAGTCGCTCACAGCTGAGCTTTCACACTATATTCCTGCAATTCTTTCAGAGCTATAA
Protein Sequence
  • >LOC_Os03g21080.1
    MDKFAGVCQSILHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQEIFLTLDHDDLSQRAKAARILVVLICKHEFDARYQKSEDKLYIAQLYFSLIGQILDEMPVFYNLNAVEKREVLVVILQIIRNLDDMTLIKAWQQSIARTRLFFKLLEECITHFEHNKTGDSLLLGSSSRSPDAERPASPKYSDRLSPSVNAYLSEASRHEIRPQGTPENGYMWNRVSPQLSSPNQPYSLREALAQAQSSRIGSTARALRESLHPVLRQKLELWEENLSTAVSLEVLGIIDKFSVAAASRSITTDYAKLDCVTSVLMGLLSRSQPLAFWKAFLPVVYNIFNLHGATLMARENDRFLKQIAFHLLRLAVFRNDSIRKRAVVGLQILVRNSFNYFKNTTRLRVMLTITLSELMSDVQVTQMKSDGSLEESGETRCLRKSLEEMADVRSKDLLKDCGLPVNALEAAPEGSTDNRWSWVEVKHLSKCLVQALDAGLEHALLGSEMTLDRYAAAEGFYKLAMAYAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVGRNDAVWSKEHVASLCKICPIVNTDVSSEASAAEVEGYGASKLTVDSAVKYLQLANKLFAQAELYHFCASIQELIIPVYKSRRAYGHLAKCHTSLKDIYESILEQEASPIPFIDATYYRVGFYGERFGKLNKKEYVFREPRDVRLGDIMEKLSHIYEAKMDGNHTLHIIPDSRQVNADELQPGVCYLQITAVDPVMEDEDLGSRRERIFSLSTGTVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVIKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL*