Information report for OsNAP
Gene Details
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Functional Descriptions
- OsNAP-overexpressing transgenic plants displayed an accelerated leaf senescence phenotype at the grain-filling stage, which might be caused by the elevated JA levels and the increased expression of the JA biosynthesis-related genes LOX2 and AOC1, and showed enhanced tolerance ability to MeJA treatment at the seedling stage.
- Analysis of the OsNAP transcript levels in rice showed that this gene was significantly induced by ABA and abiotic stresses, including high salinity, drought and low temperature.
- The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway.
- RESULTS: In this study, we reported a member of the NAC transcription factor family named OsNAP whose expression is associated with leaf senescence, and investigated its preliminary function during the process of leaf senescence.
- The results of qRT-PCR showed that the OsNAP transcripts were accumulated gradually in response to leaf senescence and treatment with methyl jasmonic acid (MeJA).
- Nevertheless, the leaf senescence process was delayed in OsNAP RNAi transgenic plants with a dramatic drop in JA levels and with decreased expression levels of the JA biosynthesis-related genes AOS2, AOC1 and OPR7.
- CONCLUSIONS: These results suggest that OsNAP acts as a positive regulator of leaf senescence and this regulation may occur via the JA pathway.
- Rice plants overexpressing OsNAP did not show growth retardation, but showed a significantly reduced rate of water loss, enhanced tolerance to high salinity, drought and low temperature at the vegetative stage, and improved yield under drought stress at the flowering stage.
- Our data suggest that OsNAP functions as a transcriptional activator that plays a role in mediating abiotic stress responses in rice.
- OsNAP is a member of the NAC transcription factor family; it is localized in the nucleus, and shows transcriptional activator activity in yeast.
- Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59).
- OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice.
- Additionally, reduced OsNAP expression leads to delayed leaf senescence and an extended grain-filling period, resulting in a 6.3% and 10.3% increase in the grain yield of two independent representative RNAi lines, respectively.
- Thus, fine-tuning OsNAP expression should be a useful strategy for improving rice yield in the future.
Functional Keywords
- grain , ABA , senescence , ja , yield , flower , abiotic-stress , transcription-factor , leaf , salinity , drought , seedling , jasmonic , temperature , growth , jasmonic-acid , vegetative , abscisic-acid , leaf-senescence , abscisic-acid-biosynthesis , grain-filling
Literature and News
- The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway . DOI: 10.1093/pcp/pct204 ; PMID: 24399239
- Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence . DOI: 10.1186/1471-2229-13-132 ; PMID: 24028154
- OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice . DOI: 10.1073/pnas.1321568111 ; PMID: 24951508
Gene Resources
- UniProt: A0A0P0VX00
- EMBL: AC126222
- AlphaFoldDB: A0A0P0VX00
- EnsemblPlants: Os03t0327800-01
- Gramene: Os03t0327800-01
- KEGG: osa:4332717
- Orthologous matrix: GHPVMNH
- InterPro: IPR003441, IPR036093
- PANTHER: PTHR31719, PTHR31719:SF201
- SUPFAM: SSF101941
- PROSITE: PS51005
- Gene3D: 2.170.150.80
- OrthoDB: A0A0P0VX00
- SWISS-MODEL: A0A0P0VX00
Homologs
- Brachypodium distachyon: Bradi1g63600.1.p
- Hordeum vulgare: MLOC_65101.1, HvNAC005
- Panicum virgatum: Pavir.9NG600600.1.p, Pavir.9KG092600.1.p
- Setaria italica: Seita.9G417600.1.p
- Setaria viridis: Sevir.9G421600.1.p
- Triticum aestivum: Traes_4DL_F0BE36C9D.1, Traes_4AS_DBDA5E9C6.1
Sequences
cDNA Sequence
- >LOC_Os03g21060.1
GCGCGCATAAAAGGAGCCCCTCCCCTCCCGTCCCCAAAAATTCGAGCCCTCCTCCATCTCTCGCCTCCTCTTGATACAAGCTCGACCCTGATAGATCTCATAGATCATAGCTGAGAGGGGATCGAGTGGCTTGATCTTGGAAGTGAGCAGCTATAGCTAGATAGGTTTTGCTTGTGCAGGGAGGTGTGTGTTGTGTGTGGGTGACTGCCGGAGACGATGGTTCTGTCGAACCCGGCGATGCTGCCGCCGGGGTTCCGGTTCCACCCGACGGACGAGGAGCTGATCGTGCACTACCTCCGCAACCGGGCCGCCTCCTCGCCGTGCCCCGTCTCCATCATCGCCGACGTCGACATCTACAAGTTCGATCCGTGGGACCTCCCATCCAAGGAGAATTACGGGGACAGGGAGTGGTACTTCTTCAGCCCGAGGGACCGCAAGTACCCGAACGGGATCCGGCCGAACCGCGCCGCGGGCTCCGGCTACTGGAAGGCCACCGGCACCGACAAGCCCATCCACAGCAGCGGCGGCGCGGCGACCAACGAGAGCGTCGGCGTCAAGAAGGCGCTCGTCTTCTACAAGGGCCGCCCGCCCAAGGGCACCAAGACCAACTGGATCATGCACGAGTACCGCCTCGCCGCCGCAGACGCCCACGCCGCCAACACCTACCGCCCCATGAAGTTCCGCAACACCTCCATGAGGCTGGATGACTGGGTGCTGTGCCGGATCTACAAGAAGAGCAGCCACGCGTCGCCGCTGGCCGTGCCGCCGCTCTCCGACCACGAGCAGGACGAGCCGTGCGCCCTGGAGGAGAACGCGCCGCTGTACGCGCCGTCGTCGTCGAGCGCCGCATCCATGATCTTGCAGGGCGCTGCCGCCGGCGCCTTCCCGTCGCTGCACGCCGCCGCCGCCGCTACGCAGAGGACGGCGATGCAGAAGATCCCTTCCATTTCCGACCTGCTCAACGAGTACTCGCTGTCGCAGCTCTTCGACGACGGCGGCGCCGCCGCCGCCGCCCCCCTGCAGGAGATGGCGAGGCAGCCCGACCACCACCACCACCAACAACAACAACACGCCCTCTTTGGCCACCCCGTCATGAACCATTTCATCGCGAACAACAGCATGGTTCAGCTCGCGCACCTGGACCCGTCCTCCTCTGCGGCCGCCTCGACGTCGGCAGGCGCCGTCGTCGAGCCGCCGGCCGTCACCGGGAAGCGCAAGAGATCATCGGACGGAGGCGAGCCGACGATCCAGGCGCTGCCTCCTGCTGCCGCGGCGGCCAAGAAGCCGAACGGTTCATGCGTTGGTGCAACTTTCCAAATAGGCAGCGCCTTGCAGGGATCGTCACTGGGACTGAGCCACCAGATGCTGCTCCACTCTAACATGGGGATGAACTGATAGCTCGTTCTTCGTACATAATACCACAGCCTGATTAAACACTGAACATAATTGCACATGGCGAATTGATGATTTGAACAGTGAGGTCTATTTCGAAAGCAAATAGGTCCAAAACGATAGATTTAAATAGTAAATATATTGGGAAAGAAATTGTACTTAATTTGTACTTGTGTGGACTAGCTAGGTCGTCCGATCATGCATAGCTATATAGTTTGTGGTGTGTATACATTTTTTCTCATGGTACTATACTACTGTGTACTCAAGCTAGCTCAGTACTAAAGATTAATTAAGCTAGATAGGGGTTGATAAGACTTTGAGATAGACCCACTAGAAGTCTGGAACATGAGATCAAGGAGAATGATGTCAAGATCATAAAGCCGGATTTATAGCTCATATTCATGTAAATCAATCAATAAGTGATTCTTTAATGGCAGCCACTCATTTTTTTCAA
CDS Sequence
- >LOC_Os03g21060.1
ATGGTTCTGTCGAACCCGGCGATGCTGCCGCCGGGGTTCCGGTTCCACCCGACGGACGAGGAGCTGATCGTGCACTACCTCCGCAACCGGGCCGCCTCCTCGCCGTGCCCCGTCTCCATCATCGCCGACGTCGACATCTACAAGTTCGATCCGTGGGACCTCCCATCCAAGGAGAATTACGGGGACAGGGAGTGGTACTTCTTCAGCCCGAGGGACCGCAAGTACCCGAACGGGATCCGGCCGAACCGCGCCGCGGGCTCCGGCTACTGGAAGGCCACCGGCACCGACAAGCCCATCCACAGCAGCGGCGGCGCGGCGACCAACGAGAGCGTCGGCGTCAAGAAGGCGCTCGTCTTCTACAAGGGCCGCCCGCCCAAGGGCACCAAGACCAACTGGATCATGCACGAGTACCGCCTCGCCGCCGCAGACGCCCACGCCGCCAACACCTACCGCCCCATGAAGTTCCGCAACACCTCCATGAGGCTGGATGACTGGGTGCTGTGCCGGATCTACAAGAAGAGCAGCCACGCGTCGCCGCTGGCCGTGCCGCCGCTCTCCGACCACGAGCAGGACGAGCCGTGCGCCCTGGAGGAGAACGCGCCGCTGTACGCGCCGTCGTCGTCGAGCGCCGCATCCATGATCTTGCAGGGCGCTGCCGCCGGCGCCTTCCCGTCGCTGCACGCCGCCGCCGCCGCTACGCAGAGGACGGCGATGCAGAAGATCCCTTCCATTTCCGACCTGCTCAACGAGTACTCGCTGTCGCAGCTCTTCGACGACGGCGGCGCCGCCGCCGCCGCCCCCCTGCAGGAGATGGCGAGGCAGCCCGACCACCACCACCACCAACAACAACAACACGCCCTCTTTGGCCACCCCGTCATGAACCATTTCATCGCGAACAACAGCATGGTTCAGCTCGCGCACCTGGACCCGTCCTCCTCTGCGGCCGCCTCGACGTCGGCAGGCGCCGTCGTCGAGCCGCCGGCCGTCACCGGGAAGCGCAAGAGATCATCGGACGGAGGCGAGCCGACGATCCAGGCGCTGCCTCCTGCTGCCGCGGCGGCCAAGAAGCCGAACGGTTCATGCGTTGGTGCAACTTTCCAAATAGGCAGCGCCTTGCAGGGATCGTCACTGGGACTGAGCCACCAGATGCTGCTCCACTCTAACATGGGGATGAACTGA
Protein Sequence
- >LOC_Os03g21060.1
MVLSNPAMLPPGFRFHPTDEELIVHYLRNRAASSPCPVSIIADVDIYKFDPWDLPSKENYGDREWYFFSPRDRKYPNGIRPNRAAGSGYWKATGTDKPIHSSGGAATNESVGVKKALVFYKGRPPKGTKTNWIMHEYRLAAADAHAANTYRPMKFRNTSMRLDDWVLCRIYKKSSHASPLAVPPLSDHEQDEPCALEENAPLYAPSSSSAASMILQGAAAGAFPSLHAAAAATQRTAMQKIPSISDLLNEYSLSQLFDDGGAAAAAPLQEMARQPDHHHHQQQQHALFGHPVMNHFIANNSMVQLAHLDPSSSAAASTSAGAVVEPPAVTGKRKRSSDGGEPTIQALPPAAAAAKKPNGSCVGATFQIGSALQGSSLGLSHQMLLHSNMGMN*