Gene Details:

Functional Descriptions:

  • In Arabidopsis, ETHYLENE INSENSITIVE3 (EIN3), which is an essential transcription factor for ethylene signaling, is regulated at the post-transcriptional level, while transcriptional regulation of EIN3 or EIN3-LIKE (EIL) genes has not been well documented.
  • Furthermore, the expression of two genes for ethylene signal transduction, ETR2 and EIN3, increased in these RNAi transgenic plants, whereas the expression of ERF3 was suppressed.
  • ETHYLENE INSENSITIVE3 (EIN3) is a transcription factor involved in the ethylene signal transduction pathway in Arabidopsis.
  • To gain a better understanding of the ethylene signal transduction pathway in rice, six EIN3-like genes (designated OsEIL1-6) were identified.
  • OsEIL1, a rice homolog of the Arabidopsis EIN3 regulates the ethylene response as a positive component.
  • The transgenic rice plants with overexpression of OsEIL1 exhibit short root, coiled primary root and slightly short shoot phenotype and elevated response to exogenous ethylene.
  • OsEBP89, an ethylene responsive element binding protein (EREBP) and OsACO1, an ACC (1-aminocyclopropane-1-carboxylic acid) oxidase gene were enhanced in the OsEIL1 overexpressing transgenic plants.
  • These results indicate that OsEIL1 is involved in ethylene signal transduction pathway and acts as a positive regulator of ethylene response in rice.
  • Ethylene signaling components OsEIN2 and the downstream transcription factor OsEIL1 positively regulated disease resistance.
  • The activation of OsEIL1 on YUC8M transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development.
  • Here, we report that the transcriptional activation of OsEIL1 on the expression of YUC8/REIN7 and indole-3-pyruvic acid (IPA)-dependent auxin biosynthesis is required for ethylene-inhibited root elongation.
  • OsEIL1 protects rice growth under NH(4) (+) nutrition by regulating OsVTC1-3-dependent N-glycosylation and root NH(4) (+) efflux.
  • We show that OsEIL1 in rice plays a contrasting role to Arabidopsis-homologous ETHYLENE-INSENSITIVE3 (AtEIN3) and maintains rice growth under NH(4) (+) by stabilizing protein N-glycosylation and reducing root NH(4) (+) efflux.
  • Our work demonstrates an important link between excessive root NH(4) (+) efflux and OsVTC1-3-mediated protein N-glycosylation in rice grown under NH(4) (+) nutrition and identifies OsEIL1 as a direct genetic regulator of OsVTC1-3 expression.
  • The repressors OsIAA21/31 exhibit earlier degradation upon ethylene treatment than the activators OsIAA1/9 in a TIR1/AFB-dependent manner, allowing OsEIL1 activation by activators OsIAA1/9 for OsTAR2 expression and signal amplification.
  • Knock out of ethylene signaling genes OsEIL1 and OsEIN2 or exogenous application of 1-methylcyclopropene (ethylene inhibitor) and ethephon (ethylene analogues) eliminated the difference of blast resistance between the 70% and 90% relative humidity conditions, suggesting that the activation of ethylene signaling contributes to humidity-modulated basal resistance against M.
  • Our results indicated that OsEBF2 can directly interact with OsEIL1 and positively regulate rice resistance to BPH.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g20790.1
    ATGGGAGGTGGTCTGGTGATGGACCAGGGCATGATGTTCCCCGGCGTGCACAACTTCGTGGATCTCCTGCAGCAGAACGGCGGCGACAAGAACCTCGGCTTCGGCGCGCTCGTGCCGCAGACGTCGTCGGGGGAGCAGTGCGTGATGGGGGAGGGCGACCTCGTGGACCCGCCGCCGGAGAGCTTCCCGGACGCCGGTGAGGACGACAGCGACGACGACGTGGAGGACATCGAGGAGCTGGAGCGCCGCATGTGGCGCGACCGCATGAAGCTGAAGCGGCTCAAGGAGCTGCAGCTGAGCCGGGGCAAGGACCCCGCGGGCGGCGTCGTGGGCGACCCGTCCAAGCCGCGGCAGTCGCAGGAGCAGGCGCGGCGGAAGAAGATGTCGCGCGCGCAGGACGGCATCCTCAAGTACATGCTCAAGATGATGGAGGTGTGCCGCGCGCAGGGGTTCGTGTACGGGATCATCCCGGAGAAGGGCAAGCCGGTGAGCGGCGCCTCCGACAACCTCCGCGGCTGGTGGAAGGAGAAGGTCCGCTTCGACCGCAACGGCCCCGCCGCCATCGCCAAGTACCAGGCCGACAACGCCGTCCCGGGCTTCGAGAGCGAGCTCGCCTCCGGCACCGGGAGCCCGCACTCGCTGCAGGAGCTGCAGGACACCACCCTCGGGTCGCTGCTCTCGGCGCTCATGCAGCACTGCGACCCTCCGCAGCGGCGGTACCCGCTCGAGAAGGGCGTCCCTCCGCCGTGGTGGCCCACCGGCGACGAGGAGTGGTGGCCGGAGCTCGGCATCCCCAAGGACCAGGGCCCGCCTCCGTACAAGAAGCCCCATGACCTCAAGAAGGCCTGGAAGGTCAGCGTGCTCACCGCTGTCATCAAGCACATGTCGCCGGACATCGAGAAGATCCGCCGGCTGGTCCGGCAGTCCAAGTGCCTCCAGGACAAGATGACCGCCAAGGAGATCTCCACCTGGCTGGCCGTCGTCAAGCAGGAAGAGGAGCTGTACCTGAAGCTGAACCCCGGTGCCCGCCCTCCGGCACCTACCGGCGGCATCACCAGCGCCATATCGTTCAACGCCAGCTCAAGTGAGTACGACGTCGACGTCGTCGACGACTGCAAGGGCGACGAGGCCGGCAACCAGAAGGCTGTTGTTGTCGCCGACCCGACCGCGTTCAACCTCGGCGCGGCTATGCTGAACGACAAGTTCCTCATGCCGGCGTCCATGAAGGAGGAGGCCACCGATGTCGAGTTCATCCAGAAGAGGAGCGCGTCTGGCGCGGAGCCTGAGCTGATGCTGAACAACCGTGTCTACACCTGCCACAATGTCCAGTGCCCGCATAGCGACTATGGATACGGGTTCCTTGACCGGAACGCGCGCAACAGCCACCAATACACTTGCAAGTACAATGATCCACTCCAGCAGAGCACGGAGAACAAGCCATCGCCACCGGCCATCTTCCCGGCAACCTACAACACGCCGAACCAGGCTCTGAACAATCTGGATTTCGGCCTGCCCATGGATGGCCAGAGGTCAATTACAGAGCTGATGAACATGTACGACAACAACTTCGTGGCCAACAAGAACCTTAGCAACGACAATGCCACGATCATGGAGAGGCCTAATGCAGTCAACCCAAGGATACAGATTGAAGAAGGCTTTTTTGGACAGGGAAGTGGCATCGGCGGCAGCAACGGAGGTGTGTTCGAAGATGTCAATGGCATGATGCAGCAACCGCAGCAGACCACCCCGGCACAGCAGCAGTTCTTCATCCGCGACGATACTCCATTCGGTAACCAGATGGGCGACATCAATGGCGCATCGGAGTTCAGGTTCGGCTCTGGTTTCAACATGTCAGGTGCCGTCGAATACCCCGGCGCAATGCAGGGCCAGCAGAAGAATGACGGCTCGAATTGGTACTACTGA
CDS Sequence
  • >LOC_Os03g20790.1
    ATGGGAGGTGGTCTGGTGATGGACCAGGGCATGATGTTCCCCGGCGTGCACAACTTCGTGGATCTCCTGCAGCAGAACGGCGGCGACAAGAACCTCGGCTTCGGCGCGCTCGTGCCGCAGACGTCGTCGGGGGAGCAGTGCGTGATGGGGGAGGGCGACCTCGTGGACCCGCCGCCGGAGAGCTTCCCGGACGCCGGTGAGGACGACAGCGACGACGACGTGGAGGACATCGAGGAGCTGGAGCGCCGCATGTGGCGCGACCGCATGAAGCTGAAGCGGCTCAAGGAGCTGCAGCTGAGCCGGGGCAAGGACCCCGCGGGCGGCGTCGTGGGCGACCCGTCCAAGCCGCGGCAGTCGCAGGAGCAGGCGCGGCGGAAGAAGATGTCGCGCGCGCAGGACGGCATCCTCAAGTACATGCTCAAGATGATGGAGGTGTGCCGCGCGCAGGGGTTCGTGTACGGGATCATCCCGGAGAAGGGCAAGCCGGTGAGCGGCGCCTCCGACAACCTCCGCGGCTGGTGGAAGGAGAAGGTCCGCTTCGACCGCAACGGCCCCGCCGCCATCGCCAAGTACCAGGCCGACAACGCCGTCCCGGGCTTCGAGAGCGAGCTCGCCTCCGGCACCGGGAGCCCGCACTCGCTGCAGGAGCTGCAGGACACCACCCTCGGGTCGCTGCTCTCGGCGCTCATGCAGCACTGCGACCCTCCGCAGCGGCGGTACCCGCTCGAGAAGGGCGTCCCTCCGCCGTGGTGGCCCACCGGCGACGAGGAGTGGTGGCCGGAGCTCGGCATCCCCAAGGACCAGGGCCCGCCTCCGTACAAGAAGCCCCATGACCTCAAGAAGGCCTGGAAGGTCAGCGTGCTCACCGCTGTCATCAAGCACATGTCGCCGGACATCGAGAAGATCCGCCGGCTGGTCCGGCAGTCCAAGTGCCTCCAGGACAAGATGACCGCCAAGGAGATCTCCACCTGGCTGGCCGTCGTCAAGCAGGAAGAGGAGCTGTACCTGAAGCTGAACCCCGGTGCCCGCCCTCCGGCACCTACCGGCGGCATCACCAGCGCCATATCGTTCAACGCCAGCTCAAGTGAGTACGACGTCGACGTCGTCGACGACTGCAAGGGCGACGAGGCCGGCAACCAGAAGGCTGTTGTTGTCGCCGACCCGACCGCGTTCAACCTCGGCGCGGCTATGCTGAACGACAAGTTCCTCATGCCGGCGTCCATGAAGGAGGAGGCCACCGATGTCGAGTTCATCCAGAAGAGGAGCGCGTCTGGCGCGGAGCCTGAGCTGATGCTGAACAACCGTGTCTACACCTGCCACAATGTCCAGTGCCCGCATAGCGACTATGGATACGGGTTCCTTGACCGGAACGCGCGCAACAGCCACCAATACACTTGCAAGTACAATGATCCACTCCAGCAGAGCACGGAGAACAAGCCATCGCCACCGGCCATCTTCCCGGCAACCTACAACACGCCGAACCAGGCTCTGAACAATCTGGATTTCGGCCTGCCCATGGATGGCCAGAGGTCAATTACAGAGCTGATGAACATGTACGACAACAACTTCGTGGCCAACAAGAACCTTAGCAACGACAATGCCACGATCATGGAGAGGCCTAATGCAGTCAACCCAAGGATACAGATTGAAGAAGGCTTTTTTGGACAGGGAAGTGGCATCGGCGGCAGCAACGGAGGTGTGTTCGAAGATGTCAATGGCATGATGCAGCAACCGCAGCAGACCACCCCGGCACAGCAGCAGTTCTTCATCCGCGACGATACTCCATTCGGTAACCAGATGGGCGACATCAATGGCGCATCGGAGTTCAGGTTCGGCTCTGGTTTCAACATGTCAGGTGCCGTCGAATACCCCGGCGCAATGCAGGGCCAGCAGAAGAATGACGGCTCGAATTGGTACTACTGA
Protein Sequence
  • >LOC_Os03g20790.1
    MGGGLVMDQGMMFPGVHNFVDLLQQNGGDKNLGFGALVPQTSSGEQCVMGEGDLVDPPPESFPDAGEDDSDDDVEDIEELERRMWRDRMKLKRLKELQLSRGKDPAGGVVGDPSKPRQSQEQARRKKMSRAQDGILKYMLKMMEVCRAQGFVYGIIPEKGKPVSGASDNLRGWWKEKVRFDRNGPAAIAKYQADNAVPGFESELASGTGSPHSLQELQDTTLGSLLSALMQHCDPPQRRYPLEKGVPPPWWPTGDEEWWPELGIPKDQGPPPYKKPHDLKKAWKVSVLTAVIKHMSPDIEKIRRLVRQSKCLQDKMTAKEISTWLAVVKQEEELYLKLNPGARPPAPTGGITSAISFNASSSEYDVDVVDDCKGDEAGNQKAVVVADPTAFNLGAAMLNDKFLMPASMKEEATDVEFIQKRSASGAEPELMLNNRVYTCHNVQCPHSDYGYGFLDRNARNSHQYTCKYNDPLQQSTENKPSPPAIFPATYNTPNQALNNLDFGLPMDGQRSITELMNMYDNNFVANKNLSNDNATIMERPNAVNPRIQIEEGFFGQGSGIGGSNGGVFEDVNGMMQQPQQTTPAQQQFFIRDDTPFGNQMGDINGASEFRFGSGFNMSGAVEYPGAMQGQQKNDGSNWYY*