Information report for OsEIL1;MHZ6
Gene Details
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Functional Descriptions
- In Arabidopsis, ETHYLENE INSENSITIVE3 (EIN3), which is an essential transcription factor for ethylene signaling, is regulated at the post-transcriptional level, while transcriptional regulation of EIN3 or EIN3-LIKE (EIL) genes has not been well documented.
- Furthermore, the expression of two genes for ethylene signal transduction, ETR2 and EIN3, increased in these RNAi transgenic plants, whereas the expression of ERF3 was suppressed.
- ETHYLENE INSENSITIVE3 (EIN3) is a transcription factor involved in the ethylene signal transduction pathway in Arabidopsis.
- To gain a better understanding of the ethylene signal transduction pathway in rice, six EIN3-like genes (designated OsEIL1-6) were identified.
- OsEIL1, a rice homolog of the Arabidopsis EIN3 regulates the ethylene response as a positive component.
- The transgenic rice plants with overexpression of OsEIL1 exhibit short root, coiled primary root and slightly short shoot phenotype and elevated response to exogenous ethylene.
- OsEBP89, an ethylene responsive element binding protein (EREBP) and OsACO1, an ACC (1-aminocyclopropane-1-carboxylic acid) oxidase gene were enhanced in the OsEIL1 overexpressing transgenic plants.
- These results indicate that OsEIL1 is involved in ethylene signal transduction pathway and acts as a positive regulator of ethylene response in rice.
- Ethylene signaling components OsEIN2 and the downstream transcription factor OsEIL1 positively regulated disease resistance.
- The activation of OsEIL1 on YUC8M transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development.
- Here, we report that the transcriptional activation of OsEIL1 on the expression of YUC8/REIN7 and indole-3-pyruvic acid (IPA)-dependent auxin biosynthesis is required for ethylene-inhibited root elongation.
- OsEIL1 protects rice growth under NH(4) (+) nutrition by regulating OsVTC1-3-dependent N-glycosylation and root NH(4) (+) efflux.
- We show that OsEIL1 in rice plays a contrasting role to Arabidopsis-homologous ETHYLENE-INSENSITIVE3 (AtEIN3) and maintains rice growth under NH(4) (+) by stabilizing protein N-glycosylation and reducing root NH(4) (+) efflux.
- Our work demonstrates an important link between excessive root NH(4) (+) efflux and OsVTC1-3-mediated protein N-glycosylation in rice grown under NH(4) (+) nutrition and identifies OsEIL1 as a direct genetic regulator of OsVTC1-3 expression.
- The repressors OsIAA21/31 exhibit earlier degradation upon ethylene treatment than the activators OsIAA1/9 in a TIR1/AFB-dependent manner, allowing OsEIL1 activation by activators OsIAA1/9 for OsTAR2 expression and signal amplification.
- Knock out of ethylene signaling genes OsEIL1 and OsEIN2 or exogenous application of 1-methylcyclopropene (ethylene inhibitor) and ethephon (ethylene analogues) eliminated the difference of blast resistance between the 70% and 90% relative humidity conditions, suggesting that the activation of ethylene signaling contributes to humidity-modulated basal resistance against M.
- Our results indicated that OsEBF2 can directly interact with OsEIL1 and positively regulate rice resistance to BPH.
Functional Keywords
- transcription-factor , ethylene , primary-root , shoot , root , resistance , disease , disease-resistance , seedling , auxin , root-elongation , auxin-biosynthesis , growth , blast , blast-resistance
Literature and News
- Identification and characterization of a novel water-deficit-suppressed gene OsARD encoding an aci-reductone-dioxygenase-like protein in rice . DOI: 10.1016/j.gene.2005.07.017 ; PMID: 16169685
- Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling . DOI: 10.1007/s00438-009-0483-1 ; PMID: 19798512
- OsEIL1, a rice homolog of the Arabidopsis EIN3 regulates the ethylene response as a positive component . DOI: 10.1007/s11103-005-6184-1 ; PMID: 16786297
- MAOHUZI6/ETHYLENE INSENSITIVE3-LIKE1 and ETHYLENE INSENSITIVE3-LIKE2 Regulate Ethylene Response of Roots and Coleoptiles and Negatively Affect Salt Tolerance in Rice . DOI: 10.1104/pp.15.00353 ; PMID: 25995326
- Activation of ethylene signaling pathways enhances disease resistance by regulating ROS and phytoalexin production in rice . DOI: 10.1111/tpj.13388 ; PMID: 27701783
- The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development . DOI: 10.1371/journal.pgen.1006955 ; PMID: 28829777
- OsEIL1 protects rice growth under NH4+ nutrition by regulating OsVTC1-3-dependent N-glycosylation and root NH4+ efflux . DOI: 10.1111/pce.14283 ; PMID: 35133011
- The OsEIL1-OsERF115-target gene regulatory module controls grain size and weight in rice . DOI: 10.1111/pbi.13825 ; PMID: 35403801
- Rice EIL1 interacts with OsIAAs to regulate auxin biosynthesis mediated by the tryptophan aminotransferase MHZ10/OsTAR2 during root ethylene responses . DOI: 10.1093/plcell/koac250 ; PMID: 35972379
- Dual impact of ambient humidity on the virulence of Magnaporthe oryzae and basal resistance in rice . DOI: 10.1111/pce.14452 ; PMID: 36175003
- The F-box protein OsEBF2 confers the resistance to the brown planthopper (Nilparvata lugens Stal) . DOI: 10.1016/j.plantsci.2022.111547 ; PMID: 36462682
Sequences
cDNA Sequence
- >LOC_Os03g20790.1
ATGGGAGGTGGTCTGGTGATGGACCAGGGCATGATGTTCCCCGGCGTGCACAACTTCGTGGATCTCCTGCAGCAGAACGGCGGCGACAAGAACCTCGGCTTCGGCGCGCTCGTGCCGCAGACGTCGTCGGGGGAGCAGTGCGTGATGGGGGAGGGCGACCTCGTGGACCCGCCGCCGGAGAGCTTCCCGGACGCCGGTGAGGACGACAGCGACGACGACGTGGAGGACATCGAGGAGCTGGAGCGCCGCATGTGGCGCGACCGCATGAAGCTGAAGCGGCTCAAGGAGCTGCAGCTGAGCCGGGGCAAGGACCCCGCGGGCGGCGTCGTGGGCGACCCGTCCAAGCCGCGGCAGTCGCAGGAGCAGGCGCGGCGGAAGAAGATGTCGCGCGCGCAGGACGGCATCCTCAAGTACATGCTCAAGATGATGGAGGTGTGCCGCGCGCAGGGGTTCGTGTACGGGATCATCCCGGAGAAGGGCAAGCCGGTGAGCGGCGCCTCCGACAACCTCCGCGGCTGGTGGAAGGAGAAGGTCCGCTTCGACCGCAACGGCCCCGCCGCCATCGCCAAGTACCAGGCCGACAACGCCGTCCCGGGCTTCGAGAGCGAGCTCGCCTCCGGCACCGGGAGCCCGCACTCGCTGCAGGAGCTGCAGGACACCACCCTCGGGTCGCTGCTCTCGGCGCTCATGCAGCACTGCGACCCTCCGCAGCGGCGGTACCCGCTCGAGAAGGGCGTCCCTCCGCCGTGGTGGCCCACCGGCGACGAGGAGTGGTGGCCGGAGCTCGGCATCCCCAAGGACCAGGGCCCGCCTCCGTACAAGAAGCCCCATGACCTCAAGAAGGCCTGGAAGGTCAGCGTGCTCACCGCTGTCATCAAGCACATGTCGCCGGACATCGAGAAGATCCGCCGGCTGGTCCGGCAGTCCAAGTGCCTCCAGGACAAGATGACCGCCAAGGAGATCTCCACCTGGCTGGCCGTCGTCAAGCAGGAAGAGGAGCTGTACCTGAAGCTGAACCCCGGTGCCCGCCCTCCGGCACCTACCGGCGGCATCACCAGCGCCATATCGTTCAACGCCAGCTCAAGTGAGTACGACGTCGACGTCGTCGACGACTGCAAGGGCGACGAGGCCGGCAACCAGAAGGCTGTTGTTGTCGCCGACCCGACCGCGTTCAACCTCGGCGCGGCTATGCTGAACGACAAGTTCCTCATGCCGGCGTCCATGAAGGAGGAGGCCACCGATGTCGAGTTCATCCAGAAGAGGAGCGCGTCTGGCGCGGAGCCTGAGCTGATGCTGAACAACCGTGTCTACACCTGCCACAATGTCCAGTGCCCGCATAGCGACTATGGATACGGGTTCCTTGACCGGAACGCGCGCAACAGCCACCAATACACTTGCAAGTACAATGATCCACTCCAGCAGAGCACGGAGAACAAGCCATCGCCACCGGCCATCTTCCCGGCAACCTACAACACGCCGAACCAGGCTCTGAACAATCTGGATTTCGGCCTGCCCATGGATGGCCAGAGGTCAATTACAGAGCTGATGAACATGTACGACAACAACTTCGTGGCCAACAAGAACCTTAGCAACGACAATGCCACGATCATGGAGAGGCCTAATGCAGTCAACCCAAGGATACAGATTGAAGAAGGCTTTTTTGGACAGGGAAGTGGCATCGGCGGCAGCAACGGAGGTGTGTTCGAAGATGTCAATGGCATGATGCAGCAACCGCAGCAGACCACCCCGGCACAGCAGCAGTTCTTCATCCGCGACGATACTCCATTCGGTAACCAGATGGGCGACATCAATGGCGCATCGGAGTTCAGGTTCGGCTCTGGTTTCAACATGTCAGGTGCCGTCGAATACCCCGGCGCAATGCAGGGCCAGCAGAAGAATGACGGCTCGAATTGGTACTACTGA
CDS Sequence
- >LOC_Os03g20790.1
ATGGGAGGTGGTCTGGTGATGGACCAGGGCATGATGTTCCCCGGCGTGCACAACTTCGTGGATCTCCTGCAGCAGAACGGCGGCGACAAGAACCTCGGCTTCGGCGCGCTCGTGCCGCAGACGTCGTCGGGGGAGCAGTGCGTGATGGGGGAGGGCGACCTCGTGGACCCGCCGCCGGAGAGCTTCCCGGACGCCGGTGAGGACGACAGCGACGACGACGTGGAGGACATCGAGGAGCTGGAGCGCCGCATGTGGCGCGACCGCATGAAGCTGAAGCGGCTCAAGGAGCTGCAGCTGAGCCGGGGCAAGGACCCCGCGGGCGGCGTCGTGGGCGACCCGTCCAAGCCGCGGCAGTCGCAGGAGCAGGCGCGGCGGAAGAAGATGTCGCGCGCGCAGGACGGCATCCTCAAGTACATGCTCAAGATGATGGAGGTGTGCCGCGCGCAGGGGTTCGTGTACGGGATCATCCCGGAGAAGGGCAAGCCGGTGAGCGGCGCCTCCGACAACCTCCGCGGCTGGTGGAAGGAGAAGGTCCGCTTCGACCGCAACGGCCCCGCCGCCATCGCCAAGTACCAGGCCGACAACGCCGTCCCGGGCTTCGAGAGCGAGCTCGCCTCCGGCACCGGGAGCCCGCACTCGCTGCAGGAGCTGCAGGACACCACCCTCGGGTCGCTGCTCTCGGCGCTCATGCAGCACTGCGACCCTCCGCAGCGGCGGTACCCGCTCGAGAAGGGCGTCCCTCCGCCGTGGTGGCCCACCGGCGACGAGGAGTGGTGGCCGGAGCTCGGCATCCCCAAGGACCAGGGCCCGCCTCCGTACAAGAAGCCCCATGACCTCAAGAAGGCCTGGAAGGTCAGCGTGCTCACCGCTGTCATCAAGCACATGTCGCCGGACATCGAGAAGATCCGCCGGCTGGTCCGGCAGTCCAAGTGCCTCCAGGACAAGATGACCGCCAAGGAGATCTCCACCTGGCTGGCCGTCGTCAAGCAGGAAGAGGAGCTGTACCTGAAGCTGAACCCCGGTGCCCGCCCTCCGGCACCTACCGGCGGCATCACCAGCGCCATATCGTTCAACGCCAGCTCAAGTGAGTACGACGTCGACGTCGTCGACGACTGCAAGGGCGACGAGGCCGGCAACCAGAAGGCTGTTGTTGTCGCCGACCCGACCGCGTTCAACCTCGGCGCGGCTATGCTGAACGACAAGTTCCTCATGCCGGCGTCCATGAAGGAGGAGGCCACCGATGTCGAGTTCATCCAGAAGAGGAGCGCGTCTGGCGCGGAGCCTGAGCTGATGCTGAACAACCGTGTCTACACCTGCCACAATGTCCAGTGCCCGCATAGCGACTATGGATACGGGTTCCTTGACCGGAACGCGCGCAACAGCCACCAATACACTTGCAAGTACAATGATCCACTCCAGCAGAGCACGGAGAACAAGCCATCGCCACCGGCCATCTTCCCGGCAACCTACAACACGCCGAACCAGGCTCTGAACAATCTGGATTTCGGCCTGCCCATGGATGGCCAGAGGTCAATTACAGAGCTGATGAACATGTACGACAACAACTTCGTGGCCAACAAGAACCTTAGCAACGACAATGCCACGATCATGGAGAGGCCTAATGCAGTCAACCCAAGGATACAGATTGAAGAAGGCTTTTTTGGACAGGGAAGTGGCATCGGCGGCAGCAACGGAGGTGTGTTCGAAGATGTCAATGGCATGATGCAGCAACCGCAGCAGACCACCCCGGCACAGCAGCAGTTCTTCATCCGCGACGATACTCCATTCGGTAACCAGATGGGCGACATCAATGGCGCATCGGAGTTCAGGTTCGGCTCTGGTTTCAACATGTCAGGTGCCGTCGAATACCCCGGCGCAATGCAGGGCCAGCAGAAGAATGACGGCTCGAATTGGTACTACTGA
Protein Sequence
- >LOC_Os03g20790.1
MGGGLVMDQGMMFPGVHNFVDLLQQNGGDKNLGFGALVPQTSSGEQCVMGEGDLVDPPPESFPDAGEDDSDDDVEDIEELERRMWRDRMKLKRLKELQLSRGKDPAGGVVGDPSKPRQSQEQARRKKMSRAQDGILKYMLKMMEVCRAQGFVYGIIPEKGKPVSGASDNLRGWWKEKVRFDRNGPAAIAKYQADNAVPGFESELASGTGSPHSLQELQDTTLGSLLSALMQHCDPPQRRYPLEKGVPPPWWPTGDEEWWPELGIPKDQGPPPYKKPHDLKKAWKVSVLTAVIKHMSPDIEKIRRLVRQSKCLQDKMTAKEISTWLAVVKQEEELYLKLNPGARPPAPTGGITSAISFNASSSEYDVDVVDDCKGDEAGNQKAVVVADPTAFNLGAAMLNDKFLMPASMKEEATDVEFIQKRSASGAEPELMLNNRVYTCHNVQCPHSDYGYGFLDRNARNSHQYTCKYNDPLQQSTENKPSPPAIFPATYNTPNQALNNLDFGLPMDGQRSITELMNMYDNNFVANKNLSNDNATIMERPNAVNPRIQIEEGFFGQGSGIGGSNGGVFEDVNGMMQQPQQTTPAQQQFFIRDDTPFGNQMGDINGASEFRFGSGFNMSGAVEYPGAMQGQQKNDGSNWYY*