Gene Details:

Functional Descriptions:

  • Furthermore, we have characterized a pathogen-inducible rice OsAOS2 gene (which encodes allene oxide synthase, a key enzyme in the JA biosynthetic pathway) and examined the role of endogenous JA in rice defense response through transgenic manipulation of the JA biosynthesis.
  • Sequence analysis indicated that OsAOS2 contains four common domains of the cytochrome P450 enzyme, but does not have the signal peptide for chloroplast targeting.
  • The basal level of OsAOS2 expression is very low in leaves but relatively high in the sheath, culm, and flower of rice plants.
  • We cloned the two rice AOS genes and observed that the transcript level of both OsAOS1 and OsAOS2 was enhanced by mechanical wounding, the infestation of the striped stem borer (SSB) (Chilo suppressalis) or brown planthopper (BPH) (Niaparvata lugens), and treatment with JA; however, OsAOS1 responded more rapidly to SSB infestation and JA treatment than did OsAOS2.
  • The antisense expression of OsAOS1 (as-aos1) or OsAOS2 (as-aos2) decreased levels of SSB- or BPH-induced JA, which, in turn, reduced the production of SSB-induced trypsin protease inhibitor (TrypPI) and volatiles as well as the resistance of rice to SSB.
  • These results indicate that OsAOS1 and OsAOS2 are both involved in herbivore-induced JA biosynthesis and play a vital role in determining the resistance of rice to chewing and phloem-feeding herbivores.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g12500.1
    ACGTCCAATTTCTTTCTCCAAGAAAGAACGCCGAACAAGCGCCGACGAAAAACGGTTCCTCGTTATTTCTCTCCAACGCGCGCGCACGGGCTATTTCTACCCCCGCCGCCGAGTAGTTAGGCGTCCAAAGTTTCGGGAGTTCATCGTGAACGGTGATCGATCCAAAGAACAACAACTCGGAGCAAAGCTAGCTAACGAAGTTAGTTCCAAACAAAGCTAGCTTATCGCGATTAGCTAGCTAGAAGAGAGTTAGCTAGGCGCCATGGAGCTAGGCGTGCCACTGCCACGACGGCCCGTGCCCGGTAGCTACGGCGTGCCGTTCGTCTCGGCGGTGCGCGACCGCCTCGATTTCTACTACTTGCAGGGGCAGGACAAGTACTTCGAGTCGCGCGCCGAGAGGTACGGCTCCACCGTCGTCCGCATCAACGTCCCGCCTGGCCCATTCATGGCGCGCGACCCCCGCGTGGTGGCGCTCCTCGACGCCAAGAGCTTCCCCGTCCTCTTCGACGTCGCCAAGGTCGAGAAGCGGGACGTGTTCACCGGCACGTTCATGCCGTCCACCTCCCTCACCGGCGGCTACCGCGTCTGCGCCTACCTCGACCCGTCCGAGCCCAACCACGCCAAGATCAAGCAGCTGCTCCTCTCCCTCCTGGTCTCTCGCAAGGACGCCTTCGTCCCGGTCTTCCGCTCCAACTTCGGCGCGCTCCTCGACACCGTCGAGTCGCAGCTCGCGAGCGGCGGCGGCAAGTCCGACTTCACCGCCCTCAACGATGCCACCTCCTTCGAGTTCATCGGCGAGGCGTACTTCGGCGTGCGTCCCTCCGCGTCGAGCTCCCTCGGCACCGGCGGGCCGACCAAGGCCGCCCTGTGGCTCCTATGGCAGCTCGCCCCGCTCACCACGCTCGGCCTGCCCATGATCATCGAGGATCCGCTCCTCCACACGCTGCCGCTGCCACCCTTCCTCATCAGCTCCGACTACAAGGCGCTGTACGCGTACTTCGCCGCCGCGGCGTCGCAGGCGCTCGACGCCGCCGAGGGCCTTGGCCTGTCGCGGGAGGAGGCCTGCCACAACCTGCTGTTCGCGACGGTGTTCAACAGCTACGGCGGCTTCAAGCTGCTGCTCCCGCAGATCCTGTCGCGCGTCGCGCAGGCCGGCGAGAAGCTCCACGAGAGGCTCGCCGCGGAGATACGGAGCGCGGTGGCCGACGCCGGCGGCAACGTGACGCTGGCCGCTCTGGAGAAGATGGAGCTGACCAGGTCGGTGGTGTGGGAGGCGCTGCGGCTGGACCCGCCGGTCAGGTTCCAGTACGGGCGCGCCAAGGCCGACCTGGAGATCGAGAGCCACGACGCGTCGTTCGCGATCAAGAAGGGGGAGATGCTGTTCGGCTACCAGCCGTGCGCCACCAGGGACCCGCGGGTGTTCGGCGCCACGGCGAGGGAGTTCGTCGGCGACCGGTTCGTCGGCGAGGAGGGGAGGAAGCTGCTGCAATACGTGTACTGGTCGAATGGGCGAGAGACGGAGAACCCTAGCGTTGACAACAAGCAGTGCCCCGGCAAGAACCTGGTGGTGCTCGTCGGAAGGCTGTTGCTCGTCGAGCTCTTCCTCCGGTACGACACCTTCACCGCCGAGGCCGGCAAAAAGGTGGTCATCACCGGGGTCACCAAAGCTTCAACCTCCGCCGTCAATCGTACTGCTTAAGCCGGCCATCACTTCAGGCGTGGGTCGTCGATCATCCACTCAACCAGCTCCGTCCTACGCATATGCAATAAGAAATACTCGTACCTGAATTCTTGCATGGCTAAACACTACTAATAAGTGTATGTATTCTGTTTATTTGTTCGATTCCATATTTGTAATTTTGTTGTTATTGTTTGCTGTTGAATTACGATTGTGTATTTTACAGGGCTGACAGTGTCTTTAAACACCTCTAATATGTATCGGATCAATCTGCCCTAGTCATGTCTAATTTGTGAAGATCCACTTTCTTTAATTATTCAATCATGCATTTTTTTTTCTTCTTT
CDS Sequence
  • >LOC_Os03g12500.1
    ATGGAGCTAGGCGTGCCACTGCCACGACGGCCCGTGCCCGGTAGCTACGGCGTGCCGTTCGTCTCGGCGGTGCGCGACCGCCTCGATTTCTACTACTTGCAGGGGCAGGACAAGTACTTCGAGTCGCGCGCCGAGAGGTACGGCTCCACCGTCGTCCGCATCAACGTCCCGCCTGGCCCATTCATGGCGCGCGACCCCCGCGTGGTGGCGCTCCTCGACGCCAAGAGCTTCCCCGTCCTCTTCGACGTCGCCAAGGTCGAGAAGCGGGACGTGTTCACCGGCACGTTCATGCCGTCCACCTCCCTCACCGGCGGCTACCGCGTCTGCGCCTACCTCGACCCGTCCGAGCCCAACCACGCCAAGATCAAGCAGCTGCTCCTCTCCCTCCTGGTCTCTCGCAAGGACGCCTTCGTCCCGGTCTTCCGCTCCAACTTCGGCGCGCTCCTCGACACCGTCGAGTCGCAGCTCGCGAGCGGCGGCGGCAAGTCCGACTTCACCGCCCTCAACGATGCCACCTCCTTCGAGTTCATCGGCGAGGCGTACTTCGGCGTGCGTCCCTCCGCGTCGAGCTCCCTCGGCACCGGCGGGCCGACCAAGGCCGCCCTGTGGCTCCTATGGCAGCTCGCCCCGCTCACCACGCTCGGCCTGCCCATGATCATCGAGGATCCGCTCCTCCACACGCTGCCGCTGCCACCCTTCCTCATCAGCTCCGACTACAAGGCGCTGTACGCGTACTTCGCCGCCGCGGCGTCGCAGGCGCTCGACGCCGCCGAGGGCCTTGGCCTGTCGCGGGAGGAGGCCTGCCACAACCTGCTGTTCGCGACGGTGTTCAACAGCTACGGCGGCTTCAAGCTGCTGCTCCCGCAGATCCTGTCGCGCGTCGCGCAGGCCGGCGAGAAGCTCCACGAGAGGCTCGCCGCGGAGATACGGAGCGCGGTGGCCGACGCCGGCGGCAACGTGACGCTGGCCGCTCTGGAGAAGATGGAGCTGACCAGGTCGGTGGTGTGGGAGGCGCTGCGGCTGGACCCGCCGGTCAGGTTCCAGTACGGGCGCGCCAAGGCCGACCTGGAGATCGAGAGCCACGACGCGTCGTTCGCGATCAAGAAGGGGGAGATGCTGTTCGGCTACCAGCCGTGCGCCACCAGGGACCCGCGGGTGTTCGGCGCCACGGCGAGGGAGTTCGTCGGCGACCGGTTCGTCGGCGAGGAGGGGAGGAAGCTGCTGCAATACGTGTACTGGTCGAATGGGCGAGAGACGGAGAACCCTAGCGTTGACAACAAGCAGTGCCCCGGCAAGAACCTGGTGGTGCTCGTCGGAAGGCTGTTGCTCGTCGAGCTCTTCCTCCGGTACGACACCTTCACCGCCGAGGCCGGCAAAAAGGTGGTCATCACCGGGGTCACCAAAGCTTCAACCTCCGCCGTCAATCGTACTGCTTAA
Protein Sequence
  • >LOC_Os03g12500.1
    MELGVPLPRRPVPGSYGVPFVSAVRDRLDFYYLQGQDKYFESRAERYGSTVVRINVPPGPFMARDPRVVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLLLSLLVSRKDAFVPVFRSNFGALLDTVESQLASGGGKSDFTALNDATSFEFIGEAYFGVRPSASSSLGTGGPTKAALWLLWQLAPLTTLGLPMIIEDPLLHTLPLPPFLISSDYKALYAYFAAAASQALDAAEGLGLSREEACHNLLFATVFNSYGGFKLLLPQILSRVAQAGEKLHERLAAEIRSAVADAGGNVTLAALEKMELTRSVVWEALRLDPPVRFQYGRAKADLEIESHDASFAIKKGEMLFGYQPCATRDPRVFGATAREFVGDRFVGEEGRKLLQYVYWSNGRETENPSVDNKQCPGKNLVVLVGRLLLVELFLRYDTFTAEAGKKVVITGVTKASTSAVNRTA*