Information report for OsAOS2;AOS
Gene Details
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Functional Descriptions
- Furthermore, we have characterized a pathogen-inducible rice OsAOS2 gene (which encodes allene oxide synthase, a key enzyme in the JA biosynthetic pathway) and examined the role of endogenous JA in rice defense response through transgenic manipulation of the JA biosynthesis.
- Sequence analysis indicated that OsAOS2 contains four common domains of the cytochrome P450 enzyme, but does not have the signal peptide for chloroplast targeting.
- The basal level of OsAOS2 expression is very low in leaves but relatively high in the sheath, culm, and flower of rice plants.
- We cloned the two rice AOS genes and observed that the transcript level of both OsAOS1 and OsAOS2 was enhanced by mechanical wounding, the infestation of the striped stem borer (SSB) (Chilo suppressalis) or brown planthopper (BPH) (Niaparvata lugens), and treatment with JA; however, OsAOS1 responded more rapidly to SSB infestation and JA treatment than did OsAOS2.
- The antisense expression of OsAOS1 (as-aos1) or OsAOS2 (as-aos2) decreased levels of SSB- or BPH-induced JA, which, in turn, reduced the production of SSB-induced trypsin protease inhibitor (TrypPI) and volatiles as well as the resistance of rice to SSB.
- These results indicate that OsAOS1 and OsAOS2 are both involved in herbivore-induced JA biosynthesis and play a vital role in determining the resistance of rice to chewing and phloem-feeding herbivores.
Functional Keywords
- ja , defense , chloroplast , defense-response , culm , flower , sheath , stem , resistance , JA , brown-planthopper , JA-biosynthesis
Literature and News
- Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice . DOI: 10.1007/s00425-012-1698-7 ; PMID: 22798060
- Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection . DOI: 10.1094/MPMI-19-1127 ; PMID: 17022177
- Phytochrome-mediated transcriptional up-regulation of ALLENE OXIDE SYNTHASE in rice seedlings . DOI: 10.1093/pcp/pch025 ; PMID: 14988482
- Application of jasmonic acid at the stage of visible brown necrotic spots in Magnaporthe oryzae infection as a novel and environment-friendly control strategy for rice blast disease . DOI: 10.1007/s00709-020-01591-0 ; PMID: 33417037
- Both Allene Oxide Synthases Genes Are Involved in the Biosynthesis of Herbivore-Induced Jasmonic Acid and Herbivore Resistance in Rice . DOI: 10.3390/plants10030442 ; PMID: 33652695
- OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes . DOI: 10.1104/pp.112.205385 ; PMID: 23296688
- Jasmonic acid contributes to rice resistance against Magnaporthe oryzae . DOI: 10.1186/s12870-022-03948-4 ; PMID: 36539712
Gene Resources
- UniProt: Q7XYS3
- EMBL: AC107226, AK061758, AP008209
- AlphaFoldDB: Q7XYS3
- EnsemblPlants: Os03t0225900-01, Os03t0225900-02
- Gramene: Os03t0225900-01, Os03t0225900-02
- KEGG: dosa:Os03g0225900
- Orthologous matrix: VANKQCP
- InterPro: IPR001128, IPR036396
- PANTHER: PTHR24286, PTHR24286:SF302
- SUPFAM: SSF48264
- Gene3D: 1.10.630.10
- OrthoDB: Q7XYS3
- SWISS-MODEL: Q7XYS3
- Conserved Domain Database: cd11071
- eggNOG: ENOG502QQNS
Sequences
cDNA Sequence
- >LOC_Os03g12500.1
ACGTCCAATTTCTTTCTCCAAGAAAGAACGCCGAACAAGCGCCGACGAAAAACGGTTCCTCGTTATTTCTCTCCAACGCGCGCGCACGGGCTATTTCTACCCCCGCCGCCGAGTAGTTAGGCGTCCAAAGTTTCGGGAGTTCATCGTGAACGGTGATCGATCCAAAGAACAACAACTCGGAGCAAAGCTAGCTAACGAAGTTAGTTCCAAACAAAGCTAGCTTATCGCGATTAGCTAGCTAGAAGAGAGTTAGCTAGGCGCCATGGAGCTAGGCGTGCCACTGCCACGACGGCCCGTGCCCGGTAGCTACGGCGTGCCGTTCGTCTCGGCGGTGCGCGACCGCCTCGATTTCTACTACTTGCAGGGGCAGGACAAGTACTTCGAGTCGCGCGCCGAGAGGTACGGCTCCACCGTCGTCCGCATCAACGTCCCGCCTGGCCCATTCATGGCGCGCGACCCCCGCGTGGTGGCGCTCCTCGACGCCAAGAGCTTCCCCGTCCTCTTCGACGTCGCCAAGGTCGAGAAGCGGGACGTGTTCACCGGCACGTTCATGCCGTCCACCTCCCTCACCGGCGGCTACCGCGTCTGCGCCTACCTCGACCCGTCCGAGCCCAACCACGCCAAGATCAAGCAGCTGCTCCTCTCCCTCCTGGTCTCTCGCAAGGACGCCTTCGTCCCGGTCTTCCGCTCCAACTTCGGCGCGCTCCTCGACACCGTCGAGTCGCAGCTCGCGAGCGGCGGCGGCAAGTCCGACTTCACCGCCCTCAACGATGCCACCTCCTTCGAGTTCATCGGCGAGGCGTACTTCGGCGTGCGTCCCTCCGCGTCGAGCTCCCTCGGCACCGGCGGGCCGACCAAGGCCGCCCTGTGGCTCCTATGGCAGCTCGCCCCGCTCACCACGCTCGGCCTGCCCATGATCATCGAGGATCCGCTCCTCCACACGCTGCCGCTGCCACCCTTCCTCATCAGCTCCGACTACAAGGCGCTGTACGCGTACTTCGCCGCCGCGGCGTCGCAGGCGCTCGACGCCGCCGAGGGCCTTGGCCTGTCGCGGGAGGAGGCCTGCCACAACCTGCTGTTCGCGACGGTGTTCAACAGCTACGGCGGCTTCAAGCTGCTGCTCCCGCAGATCCTGTCGCGCGTCGCGCAGGCCGGCGAGAAGCTCCACGAGAGGCTCGCCGCGGAGATACGGAGCGCGGTGGCCGACGCCGGCGGCAACGTGACGCTGGCCGCTCTGGAGAAGATGGAGCTGACCAGGTCGGTGGTGTGGGAGGCGCTGCGGCTGGACCCGCCGGTCAGGTTCCAGTACGGGCGCGCCAAGGCCGACCTGGAGATCGAGAGCCACGACGCGTCGTTCGCGATCAAGAAGGGGGAGATGCTGTTCGGCTACCAGCCGTGCGCCACCAGGGACCCGCGGGTGTTCGGCGCCACGGCGAGGGAGTTCGTCGGCGACCGGTTCGTCGGCGAGGAGGGGAGGAAGCTGCTGCAATACGTGTACTGGTCGAATGGGCGAGAGACGGAGAACCCTAGCGTTGACAACAAGCAGTGCCCCGGCAAGAACCTGGTGGTGCTCGTCGGAAGGCTGTTGCTCGTCGAGCTCTTCCTCCGGTACGACACCTTCACCGCCGAGGCCGGCAAAAAGGTGGTCATCACCGGGGTCACCAAAGCTTCAACCTCCGCCGTCAATCGTACTGCTTAAGCCGGCCATCACTTCAGGCGTGGGTCGTCGATCATCCACTCAACCAGCTCCGTCCTACGCATATGCAATAAGAAATACTCGTACCTGAATTCTTGCATGGCTAAACACTACTAATAAGTGTATGTATTCTGTTTATTTGTTCGATTCCATATTTGTAATTTTGTTGTTATTGTTTGCTGTTGAATTACGATTGTGTATTTTACAGGGCTGACAGTGTCTTTAAACACCTCTAATATGTATCGGATCAATCTGCCCTAGTCATGTCTAATTTGTGAAGATCCACTTTCTTTAATTATTCAATCATGCATTTTTTTTTCTTCTTT
CDS Sequence
- >LOC_Os03g12500.1
ATGGAGCTAGGCGTGCCACTGCCACGACGGCCCGTGCCCGGTAGCTACGGCGTGCCGTTCGTCTCGGCGGTGCGCGACCGCCTCGATTTCTACTACTTGCAGGGGCAGGACAAGTACTTCGAGTCGCGCGCCGAGAGGTACGGCTCCACCGTCGTCCGCATCAACGTCCCGCCTGGCCCATTCATGGCGCGCGACCCCCGCGTGGTGGCGCTCCTCGACGCCAAGAGCTTCCCCGTCCTCTTCGACGTCGCCAAGGTCGAGAAGCGGGACGTGTTCACCGGCACGTTCATGCCGTCCACCTCCCTCACCGGCGGCTACCGCGTCTGCGCCTACCTCGACCCGTCCGAGCCCAACCACGCCAAGATCAAGCAGCTGCTCCTCTCCCTCCTGGTCTCTCGCAAGGACGCCTTCGTCCCGGTCTTCCGCTCCAACTTCGGCGCGCTCCTCGACACCGTCGAGTCGCAGCTCGCGAGCGGCGGCGGCAAGTCCGACTTCACCGCCCTCAACGATGCCACCTCCTTCGAGTTCATCGGCGAGGCGTACTTCGGCGTGCGTCCCTCCGCGTCGAGCTCCCTCGGCACCGGCGGGCCGACCAAGGCCGCCCTGTGGCTCCTATGGCAGCTCGCCCCGCTCACCACGCTCGGCCTGCCCATGATCATCGAGGATCCGCTCCTCCACACGCTGCCGCTGCCACCCTTCCTCATCAGCTCCGACTACAAGGCGCTGTACGCGTACTTCGCCGCCGCGGCGTCGCAGGCGCTCGACGCCGCCGAGGGCCTTGGCCTGTCGCGGGAGGAGGCCTGCCACAACCTGCTGTTCGCGACGGTGTTCAACAGCTACGGCGGCTTCAAGCTGCTGCTCCCGCAGATCCTGTCGCGCGTCGCGCAGGCCGGCGAGAAGCTCCACGAGAGGCTCGCCGCGGAGATACGGAGCGCGGTGGCCGACGCCGGCGGCAACGTGACGCTGGCCGCTCTGGAGAAGATGGAGCTGACCAGGTCGGTGGTGTGGGAGGCGCTGCGGCTGGACCCGCCGGTCAGGTTCCAGTACGGGCGCGCCAAGGCCGACCTGGAGATCGAGAGCCACGACGCGTCGTTCGCGATCAAGAAGGGGGAGATGCTGTTCGGCTACCAGCCGTGCGCCACCAGGGACCCGCGGGTGTTCGGCGCCACGGCGAGGGAGTTCGTCGGCGACCGGTTCGTCGGCGAGGAGGGGAGGAAGCTGCTGCAATACGTGTACTGGTCGAATGGGCGAGAGACGGAGAACCCTAGCGTTGACAACAAGCAGTGCCCCGGCAAGAACCTGGTGGTGCTCGTCGGAAGGCTGTTGCTCGTCGAGCTCTTCCTCCGGTACGACACCTTCACCGCCGAGGCCGGCAAAAAGGTGGTCATCACCGGGGTCACCAAAGCTTCAACCTCCGCCGTCAATCGTACTGCTTAA
Protein Sequence
- >LOC_Os03g12500.1
MELGVPLPRRPVPGSYGVPFVSAVRDRLDFYYLQGQDKYFESRAERYGSTVVRINVPPGPFMARDPRVVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLLLSLLVSRKDAFVPVFRSNFGALLDTVESQLASGGGKSDFTALNDATSFEFIGEAYFGVRPSASSSLGTGGPTKAALWLLWQLAPLTTLGLPMIIEDPLLHTLPLPPFLISSDYKALYAYFAAAASQALDAAEGLGLSREEACHNLLFATVFNSYGGFKLLLPQILSRVAQAGEKLHERLAAEIRSAVADAGGNVTLAALEKMELTRSVVWEALRLDPPVRFQYGRAKADLEIESHDASFAIKKGEMLFGYQPCATRDPRVFGATAREFVGDRFVGEEGRKLLQYVYWSNGRETENPSVDNKQCPGKNLVVLVGRLLLVELFLRYDTFTAEAGKKVVITGVTKASTSAVNRTA*