GLN1;2;GS1;2

Gene Details

MSU gene ID: LOC_Os03g12290
RAPdb gene ID: Os03g0223400
Gene Symbol: GLN1 2 GS1 2 OsGS1 2
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

The results indicate that GS1;1 is important for normal growth and grain filling in rice; GS1;2 and GS1;3 were not able to compensate for GS1;1 function.
In both the genotypes, both increase and decline in seed protein contents matched with the expressions levels of OsAMT1;1, OsGS1;1 and OsGS1;2 in the flag leaves during grain filling stage indicating that high nitrogen nutrition in KN3119 probably causes the repression of these genes which might be important during grain filling.
By contrast, OsGLN1;2 was abundantly expressed in the same cell layers under nitrogen-sufficient conditions, replenishing the loss of OsGLN1;1 following ammonium treatment.
OsGS1;1 was expressed in all organs tested with higher expression in leaf blades, while OsGS1;2, and OsGS1;3 were expressed mainly in roots and spikelets, respectively.
Among the three isoenzymes of the cytosolic GS1 gene family in rice, OsGLN1;1 and OsGLN1;2 were abundantly expressed in roots.
Low-affinity forms of GS1 comparable to those in Arabidopsis (GLN1;2 and GLN1;3) were absent in rice roots.
The OsGLN1;1 and OsGLN1;2 transcripts showed reciprocal responses to ammonium supply in the surface cell layers of roots.
The high-capacity Gln synthetic activities of OsGLN1;1 and OsGLN1;2 facilitate active ammonium assimilation in specific cell types in rice roots.
Although a small gene family for both GS and GOGAT is present in rice, ammonium-dependent and cell type-specific expression suggest that cytosolic GS1;2 and plastidic NADH-GOGAT1 are responsible for the primary assimilation of ammonium ions in the roots.
Although a high content of strigolactone in rice roots is known to reduce active tiller number, the reduction of outgrowth of axillary buds observed in the GS1;2 mutants was independent of the level of strigolactone.
Transcriptomic analysis using a 5 mm basal region of rice shoots showed that the GS1;2 mutants accumulated reduced amounts of mRNAs for carbon and nitrogen metabolism, including C1 unit transfer in lignin synthesis.
The lack of GS1;2 resulted in reduced accumulation of lignin.
Thus metabolic disorder caused by the lack of GS1;2 resulted in a severe reduction in the outgrowth of axillary buds and lignin deposition.
Thus, organic N assimilated by OsGS1;1 affects a broad range of metabolites and transcripts involved in maintaining metabolic homeostasis and plastid development in rice roots, whereas OsGS1;2 has a more specific role, affecting mainly amino acid homeostasis but not C metabolism.
We observed (i) abnormal sugar and organic N accumulation and (ii) significant upregulation of genes associated with photosynthesis and chlorophyll biosynthesis in the roots of Osgs1;1 but not OsGS1;2 knockout mutants.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os03g12290.1
TGGAAAACTGACCCACTATATTTTGCTCCTCCTTTTAGTTGTAAACATCCTTCTGGAAATACATATGTGCACAAGCACAGTGCTCACATAATTCATGAGTTGGACACCAGATGAAAAAAAACCCCAAGAAAATAATAAGTGGAGTAAACTTCCATCCAACTTAAGTTACCACGGTCCACGGTTAATTCCTATTGACTACGAACAAATTGTTTTACTATGTTAACCATGTTTTTTTAGACAATAGTGTCAAATTATCATATTACGTATGAATTTATGATTACACCTCTTCTTTCTCCCTGAAAATACTGCAGCATCCTCTCGATTTGAGATGATTACTTCAGTTTCTTGCCACACCAGAGGTAATCCATTCCCTTGATTTTTGTTAAAAGATAAAGATAATATAGACGCTATCTTTTTAAACGGCCAATATCATTATTAATCAAATAACTGAAAAAAAAATGTCTCTGAATGATACTTGCCCCTACTTCGCTATCCACAGTTGCGATTTGCGACTTGCAAGCACACCGTAGCTAACTTCTCTCCTTCTGGGTATAAATATCCCTCTCCTCTCCCTGTTTCTCCTCATCCCTGCTTCCTCTTCTACACCTCATTTTCCGCTTGCATCTTGCTCATTCAGATCTCTTCTGCTTTGAGCAATGGCCAACCTCACCGACCTCGTTAACCTCAACCTCAGCGACTGCAGCGACAAGATCATCGCCGAGTACATCTGGGTTGGAGGATCGGGCATAGACCTCAGGAGCAAAGCGAGGACTGTGAAAGGCCCCATCACCGATGTGAGCCAGCTGCCGAAGTGGAACTACGACGGCTCCAGCACCGGGCAGGCTCCCGGCGAGGACAGCGAAGTGATCCTCTACCCTCAAGCCATTTTCAAGGACCCGTTCAGGAGGGGCGACAACATCCTTGTGATGTGCGACTGCTACACGCCACAAGGTGAGCCAATCCCCACTAACAAGAGGCACAGTGCCGCCAAGATCTTCAGCCACCCTGATGTTGTTGCTGAGGTGCCATGGTACGGTATTGAGCAGGAGTACACACTCCTTCAAAAGGATGTGAACTGGCCCCTTGGCTGGCCAGTTGGTGGCTTCCCTGGCCCACAGGGACCATACTACTGCGCTGCCGGTGCCGAAAAGGCGTTCGGCCGCGACATCGTGGACGCCCACTACAAGGCCTGCATCTACGCCGGGATCAACATCAGTGGCATCAACGGGGAAGTCATGCCCGGCCAGTGGGAGTTCCAAGTTGGCCCGTCAGTTGGCATCGCCGCTGCTGACCAAGTGTGGGTTGCCCGCTACATCCTCGAGAGGGTCACAGAGGTGGCCGGAGTCGTGCTCTCCCTTGACCCGAAGCCGATCCCGGGTGACTGGAATGGCGCTGGTGCCCACACCAACTTCAGCACCAAGTCGATGAGGGAGCCGGGAGGCTACGAGGTGATCAAGAAGGCGATCGACAAGCTCGCGCTGAGGCACAAGGAGCACATCGCCGCCTACGGCGAGGGCAACGAGCGCCGCCTCACCGGCCGCCACGAGACCGCCGACATCAACACCTTCAAATGGGGCGTGGCGAACCGCGGCGCGTCCATCCGCGTGGGGCGCGACACGGAGAAGGAGGGCAAGGGGTACTTCGAGGACAGGAGGCCGGCGTCCAACATGGACCCATACGTCGTCACCGGCATGATCGCCGAGACCACGCTGCTGTGGAAGCAGAACTAAGCCGTCCGGCGGGCCTCTCCCGTGCATTTCTGCGCCCAAAAAAAACAAAAAAAAGAAACGTACGCGTGCGTGCATCAGCCGTGATGTGTCAACGTTGGATTTCGGTCTCGGTCTATCCTTGGCGTCGACAAGGCCCTTTGAATTTTATTACTAGTCTGTCCTGTTCACCGAGTGTCATTTTGTGTACTCTGAATAATAATGCTTTGTAAAACGGATTTGCTTTTTCCGTAGGGACGGATGTTCTTGATTTTCCTATATCGTGATTTATATATATGGAAAACCGCTGTCTTTTTTTGTTCGATCA

CDS Sequence

>LOC_Os03g12290.1
ATGGCCAACCTCACCGACCTCGTTAACCTCAACCTCAGCGACTGCAGCGACAAGATCATCGCCGAGTACATCTGGGTTGGAGGATCGGGCATAGACCTCAGGAGCAAAGCGAGGACTGTGAAAGGCCCCATCACCGATGTGAGCCAGCTGCCGAAGTGGAACTACGACGGCTCCAGCACCGGGCAGGCTCCCGGCGAGGACAGCGAAGTGATCCTCTACCCTCAAGCCATTTTCAAGGACCCGTTCAGGAGGGGCGACAACATCCTTGTGATGTGCGACTGCTACACGCCACAAGGTGAGCCAATCCCCACTAACAAGAGGCACAGTGCCGCCAAGATCTTCAGCCACCCTGATGTTGTTGCTGAGGTGCCATGGTACGGTATTGAGCAGGAGTACACACTCCTTCAAAAGGATGTGAACTGGCCCCTTGGCTGGCCAGTTGGTGGCTTCCCTGGCCCACAGGGACCATACTACTGCGCTGCCGGTGCCGAAAAGGCGTTCGGCCGCGACATCGTGGACGCCCACTACAAGGCCTGCATCTACGCCGGGATCAACATCAGTGGCATCAACGGGGAAGTCATGCCCGGCCAGTGGGAGTTCCAAGTTGGCCCGTCAGTTGGCATCGCCGCTGCTGACCAAGTGTGGGTTGCCCGCTACATCCTCGAGAGGGTCACAGAGGTGGCCGGAGTCGTGCTCTCCCTTGACCCGAAGCCGATCCCGGGTGACTGGAATGGCGCTGGTGCCCACACCAACTTCAGCACCAAGTCGATGAGGGAGCCGGGAGGCTACGAGGTGATCAAGAAGGCGATCGACAAGCTCGCGCTGAGGCACAAGGAGCACATCGCCGCCTACGGCGAGGGCAACGAGCGCCGCCTCACCGGCCGCCACGAGACCGCCGACATCAACACCTTCAAATGGGGCGTGGCGAACCGCGGCGCGTCCATCCGCGTGGGGCGCGACACGGAGAAGGAGGGCAAGGGGTACTTCGAGGACAGGAGGCCGGCGTCCAACATGGACCCATACGTCGTCACCGGCATGATCGCCGAGACCACGCTGCTGTGGAAGCAGAACTAA

Protein Sequence

>LOC_Os03g12290.1
MANLTDLVNLNLSDCSDKIIAEYIWVGGSGIDLRSKARTVKGPITDVSQLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGDNILVMCDCYTPQGEPIPTNKRHSAAKIFSHPDVVAEVPWYGIEQEYTLLQKDVNWPLGWPVGGFPGPQGPYYCAAGAEKAFGRDIVDAHYKACIYAGINISGINGEVMPGQWEFQVGPSVGIAAADQVWVARYILERVTEVAGVVLSLDPKPIPGDWNGAGAHTNFSTKSMREPGGYEVIKKAIDKLALRHKEHIAAYGEGNERRLTGRHETADINTFKWGVANRGASIRVGRDTEKEGKGYFEDRRPASNMDPYVVTGMIAETTLLWKQN*