Gene Details:
- MSU gene ID: LOC_Os03g11670
- RAPdb gene ID: Os03g0215900
- Gene Symbol: OsPPR676 OsATP4
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Disruption of OsPPR676 resulted in growth retardation of plants and partial sterility of pollens.
- In this study, OsPPR676 was confirmed to be an interacted protein with Osj10gBTF3, β-subunit of nascent polypeptide-associated complex (β-NAC), by bimolecular fluorescence complementation assays, indicating that both proteins are probably involved in the same regulatory pathway of pollen development.
- Compared with other chloroplast-rich tissues, OsPPR676 was only weakly expressed in anther, but in the Mei and YM stages of pollen development, its expression was relatively strong in the tapetum.
- OsATP4 mutants were chlorotic and had a plastid-ribosome deficiency when grown in the cold.
- Expression of the edited rps8 isoform in transgenic OsATP4 mutants complemented the cold-sensitive phenotype, indicating that a rps8 expression defect accounts for the cold sensitivity.
Function-related keywords:
- growth , pollen , sterility , development , tapetum , pollen-development , cold
Literature:
- A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice . DOI: 10.1038/s41598-017-10727-x ; PMID: 28904339
- The PPR-SMR Protein ATP4 Is Required for Editing the Chloroplast rps8 mRNA in Rice and Maize . DOI: 10.1104/pp.20.00849 ; PMID: 32928899
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os03g11670.1
AATCCACACAGAGAGAGCATCGCGCGGCATGGCTTCCCCTTCCTCCCTCCTCTCATGGCCTCACCGCGCAATCTCCCTCTCCTTCCAACCCAAGAACCCCTCTCCCTCTCCGGCCACCGCCCGTGTCTCCGTCCAAGACCCGCCGCCGCCTCCCTCCGACGCCAATCCGAGTCCGGGGAGGTCGAGCAATACGAGCAGGTACGTCTGGGTCAACCCCAACAGCCCGCGCGCCGCGGGGCTCGCCCGCGCGCGCGCCGGGTCGGGCCGACGCGCCCGCCTCGCCGCCGCGGCGGCCGCGCTTGCCGCCTGCGAGGCCGGGGAGGCGCCCGTCGCCGCGGCGCTGGAGGCCGCGTTCCCGGAACCCCCGTCCGAGCAGGACGCCGTCATCGTGCTCAACACGACCTCCGCCAGGCCCGCCGCCGTCGTGCTCGCGCTGTGGTGGTTCCTCCGGAACGCCGAGGTTCGCAAGGAGGTCATCCTCTACAACGTCGCGCTCAAGGCCCTGCGCAAGAGGCGGCGCTGGAGCGACGCCGAGGCGCTCTGGGAGGAGATGCTGCGCGAGGGCGTGCAGCCGGACAACGCCACCTTCTCGACCGTCATCAGCTGCGCGCGCGCCTGTGGCATGCCAGGCAAGGCCGTGGAGTGGTTCGAGAAGATGCCGGACTTCGGGTGCTCGCCGGACATGCTCACGTACTCGGCGGTGATCGACGCGTACGGCCGCGCCGGGGACGCCGAGACGGCCCTCCGCCTGTACGACCGCGCCCGCGCCGAGAAATGGCAGCTGGACCCCGTCATATGCGCCACGGTGATCAGGGTGCACTCGAGCAGCGGCAACTTCGACGGCGCACTCAACGTGTTCGAGGAAATGAAGGCGGCCGGCGTCAAGCCAAACCTGGTCGTGTACAACACGGTGCTGGACGCCATGGGCCGCGCGATGCGGCCGTGGGTGGTGAAGACCATCCACAGGGAGCTAGTCAGCCAGGAGGCGGTGCCCAACAAGGCGACGTACTGCTGCCTCCTCCATGCGTACACCAGGGCGCGCTACGGCGAGGACGCCATGGCCGTGTACCGGGTGATGAAGGACGAGGTGATGGACATCGACGTCGTGCTGTACAACATGCTCCTTTCGATGTGCGCCGACATCGGCTACGTCGAGGAGGCGGAGGAGATCTTCAGGGACATGAAGGCCTCCATGGATTCCCGGTCCAAGCCTGACAGCTGGAGCTACTCATCCATGGTGACCCTCTACTCCTGCACCGGCAATGTCGCGGGTGCGGAGGGCATCCTGAACGAGATGGTGGAGGCTGGCTTCAAGCCCAACATCTTCATCCTCACCTCGCTCATCCGTTGCTACGGCAAAGCGGGGCGCACCGACGACGTGGTCAGGTCATTCGCCATGCTCGAGGACCTCGGCATCACCCCGGACGACCGGTTCTGCGGCTGCCTCCTCACCGTGGCGGCCGGCACGCCGGCGGATGAGCTGGGCAAGGTGATCGGGTGCATCGACCGGAGCAGCGCGCAGCTAGGCGCCGTGGTGCGGCTCCTGGTGGACGCCGCGGCGCCCAGCGAGCCCCTGAGGGAGGCCGCCGGCGAGCTGCTGGGCGGCGCGCGCGGCGTGGTGAGGATGCCGTACTGCAACTGCCTGATGGACCTGGCGGTGAACCTGAGCCAGATGGAGAAGGCCTGCGCGCTGCTGGACGTCGCGCTGCGGCTGGGCATCTACAGCAACGTGCAGACGAGGACGCAGACGCAGTGGTCGCTGCACCTGAGGGGCCTCTCCGTCGGCGCCGCGCTGACGACGCTGCACGTCTGGATGAGCGACCTGTACGCGGCGCTGCAGGCCGGGGACGAGCTGCCGCCGCTGCTCGGGATCCACACGGGGCAGGGGAAGAACACGTACTCCTACAAGGGGCTCGCCACCGTGTTCGAGTCGCATCTCAAGGAGCTCGACGCGCCGTTCCACGAGGCGCCCGACAAGGCCGGCTGGTTCTTGACGACGAGCGTCGCGGCCAGGCACTGGCTGGAGACAAAGAAATCGGCAGAGTTAGTAGCGGTGTAAAACGCAACAATGGAGTAGGAATATATAGGATTTTTTTTTTGTTGGGCTTTTCGTAGTATCTCAATTTATGCAGGTGTTCTCAGGTTTTCTCCCTCCACAGATTTCTTCTAATGGTAGATGCTCAGACTTGCATATACTGTTGCCTCTGCTTCCATTGACAGAAGAACATCAGTCAAATTCCTCATTCAATTGAAAATAAAAAGCGCTGTTAACATTA
CDS Sequence
- >LOC_Os03g11670.1
ATGGCTTCCCCTTCCTCCCTCCTCTCATGGCCTCACCGCGCAATCTCCCTCTCCTTCCAACCCAAGAACCCCTCTCCCTCTCCGGCCACCGCCCGTGTCTCCGTCCAAGACCCGCCGCCGCCTCCCTCCGACGCCAATCCGAGTCCGGGGAGGTCGAGCAATACGAGCAGGTACGTCTGGGTCAACCCCAACAGCCCGCGCGCCGCGGGGCTCGCCCGCGCGCGCGCCGGGTCGGGCCGACGCGCCCGCCTCGCCGCCGCGGCGGCCGCGCTTGCCGCCTGCGAGGCCGGGGAGGCGCCCGTCGCCGCGGCGCTGGAGGCCGCGTTCCCGGAACCCCCGTCCGAGCAGGACGCCGTCATCGTGCTCAACACGACCTCCGCCAGGCCCGCCGCCGTCGTGCTCGCGCTGTGGTGGTTCCTCCGGAACGCCGAGGTTCGCAAGGAGGTCATCCTCTACAACGTCGCGCTCAAGGCCCTGCGCAAGAGGCGGCGCTGGAGCGACGCCGAGGCGCTCTGGGAGGAGATGCTGCGCGAGGGCGTGCAGCCGGACAACGCCACCTTCTCGACCGTCATCAGCTGCGCGCGCGCCTGTGGCATGCCAGGCAAGGCCGTGGAGTGGTTCGAGAAGATGCCGGACTTCGGGTGCTCGCCGGACATGCTCACGTACTCGGCGGTGATCGACGCGTACGGCCGCGCCGGGGACGCCGAGACGGCCCTCCGCCTGTACGACCGCGCCCGCGCCGAGAAATGGCAGCTGGACCCCGTCATATGCGCCACGGTGATCAGGGTGCACTCGAGCAGCGGCAACTTCGACGGCGCACTCAACGTGTTCGAGGAAATGAAGGCGGCCGGCGTCAAGCCAAACCTGGTCGTGTACAACACGGTGCTGGACGCCATGGGCCGCGCGATGCGGCCGTGGGTGGTGAAGACCATCCACAGGGAGCTAGTCAGCCAGGAGGCGGTGCCCAACAAGGCGACGTACTGCTGCCTCCTCCATGCGTACACCAGGGCGCGCTACGGCGAGGACGCCATGGCCGTGTACCGGGTGATGAAGGACGAGGTGATGGACATCGACGTCGTGCTGTACAACATGCTCCTTTCGATGTGCGCCGACATCGGCTACGTCGAGGAGGCGGAGGAGATCTTCAGGGACATGAAGGCCTCCATGGATTCCCGGTCCAAGCCTGACAGCTGGAGCTACTCATCCATGGTGACCCTCTACTCCTGCACCGGCAATGTCGCGGGTGCGGAGGGCATCCTGAACGAGATGGTGGAGGCTGGCTTCAAGCCCAACATCTTCATCCTCACCTCGCTCATCCGTTGCTACGGCAAAGCGGGGCGCACCGACGACGTGGTCAGGTCATTCGCCATGCTCGAGGACCTCGGCATCACCCCGGACGACCGGTTCTGCGGCTGCCTCCTCACCGTGGCGGCCGGCACGCCGGCGGATGAGCTGGGCAAGGTGATCGGGTGCATCGACCGGAGCAGCGCGCAGCTAGGCGCCGTGGTGCGGCTCCTGGTGGACGCCGCGGCGCCCAGCGAGCCCCTGAGGGAGGCCGCCGGCGAGCTGCTGGGCGGCGCGCGCGGCGTGGTGAGGATGCCGTACTGCAACTGCCTGATGGACCTGGCGGTGAACCTGAGCCAGATGGAGAAGGCCTGCGCGCTGCTGGACGTCGCGCTGCGGCTGGGCATCTACAGCAACGTGCAGACGAGGACGCAGACGCAGTGGTCGCTGCACCTGAGGGGCCTCTCCGTCGGCGCCGCGCTGACGACGCTGCACGTCTGGATGAGCGACCTGTACGCGGCGCTGCAGGCCGGGGACGAGCTGCCGCCGCTGCTCGGGATCCACACGGGGCAGGGGAAGAACACGTACTCCTACAAGGGGCTCGCCACCGTGTTCGAGTCGCATCTCAAGGAGCTCGACGCGCCGTTCCACGAGGCGCCCGACAAGGCCGGCTGGTTCTTGACGACGAGCGTCGCGGCCAGGCACTGGCTGGAGACAAAGAAATCGGCAGAGTTAGTAGCGGTGTAA
Protein Sequence
- >LOC_Os03g11670.1
MASPSSLLSWPHRAISLSFQPKNPSPSPATARVSVQDPPPPPSDANPSPGRSSNTSRYVWVNPNSPRAAGLARARAGSGRRARLAAAAAALAACEAGEAPVAAALEAAFPEPPSEQDAVIVLNTTSARPAAVVLALWWFLRNAEVRKEVILYNVALKALRKRRRWSDAEALWEEMLREGVQPDNATFSTVISCARACGMPGKAVEWFEKMPDFGCSPDMLTYSAVIDAYGRAGDAETALRLYDRARAEKWQLDPVICATVIRVHSSSGNFDGALNVFEEMKAAGVKPNLVVYNTVLDAMGRAMRPWVVKTIHRELVSQEAVPNKATYCCLLHAYTRARYGEDAMAVYRVMKDEVMDIDVVLYNMLLSMCADIGYVEEAEEIFRDMKASMDSRSKPDSWSYSSMVTLYSCTGNVAGAEGILNEMVEAGFKPNIFILTSLIRCYGKAGRTDDVVRSFAMLEDLGITPDDRFCGCLLTVAAGTPADELGKVIGCIDRSSAQLGAVVRLLVDAAAPSEPLREAAGELLGGARGVVRMPYCNCLMDLAVNLSQMEKACALLDVALRLGIYSNVQTRTQTQWSLHLRGLSVGAALTTLHVWMSDLYAALQAGDELPPLLGIHTGQGKNTYSYKGLATVFESHLKELDAPFHEAPDKAGWFLTTSVAARHWLETKKSAELVAV*