Gene Details:

Functional Descriptions:

  • Microscopic observation revealed defects in the vascular system and reduced epidermal cell size and number in the NAL21 leaf blade.
  • Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of NAL21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs.
  • The NAL21 mutant showed aberrant auxin responses in which multiple auxin response factors (ARFs) harboring upstream open reading frames (uORFs) in their 5’-untranslated region (5’-UTR) were repressed at the translational level.
  • Ribosome-targeting antibiotics resistance assay and ribosome profiling showed a significant reduction in the free 40S ribosome subunit in the NAL21 mutant.
  • Map-based cloning revealed that NAL21 encodes a ribosomal small subunit protein RPS3A.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g10340.1
    GGAGATAGGGTTTCTCCTCGCCGCTTCCACCTCTCTCGCGCCGCCGCCGCCGCCGAGCGCCTGGAGCAGCGTCAGCCATGGCCGTAGGTAAGAACAAGCGCATCTCCAAGGGGAAGAAGGGATCCAAGAAGAAGACCGTCGATCCCTTTGCTAAGAAGGACTGGTATGATATCAAGGCCCCGTCGGTGTTCAATGTGCGGAACATCGGCAAGACCCTCGTGTCCAGGACACAGGGTACAAAGATTGCTTCTGAGGGCCTAAAGCATAGAGTGTTTGAGGTCTCCTTAGCTGATCTCCAGAACGATGAGGATCAGGCGTACAGGAAGATCAGACTTCGTGCTGAGGATGTGCAAGGGAAGAACGTGCTCACCAACTTTTGGGGCATGTCGTTTACTACCGATAAGCTCAGATCACTTGTTAAGAAGTGGCAGACACTGATTGAGGCTCATGTGGATGTCAAAACCACAGACGGCTACATGCTGCGTCTGTTCTGTATCGGCTTCACCAAGCGGCGGCCTAACCAGGTGAAGAGGACTTGCTATGCTCAAGCGAGTCAGATCAGACAGATTCGTCGCAAGATGGTTGAAATCATGGCCAACCAGGCTTCAAGCTGTGATTTGAAAGAGCTAGTTTCAAAGTTCATTCCTGAAGTTATTGGGAAGGAAATTGAGAAGGCGACATCCAGTATTTTCCCCCTTCAGAATGTGTTTGTCCGCAAAGTGAAGATTCTAAAGGCCCCGAAATTTGACCTGGGGAAGCTCATGGAGGTGCATGGTGACTATGCCAAGGAGGACATCGGTACGAAGCTTGATAGGCCTGCAGAAGATGAGGCCATGGCCGGGCAGGAGGTCGCTGCTGCCGAGTAGAGATTATCACTTATTTTGCTGCCCGTTTATTTAGACAACAAGTAAACAGCTATCTAGTTGTGGATCGTGAACATCCTAGCCTGTGTCAATGGCAGATATTCTGAATAGTTTGTTGGGATTACTTGCCAGCTGCATATTTGCTTTCTGATGAGAAATGAATGTTTTTGAGTCTATGCGTTTGCTTTGTTTGGTCTTGATGATGAAGTTAAGAATTTTGAAGTAATTGTTTGGTCTGGAGTAGTTTTGATCCTGGCAGTCTTTAATTTCTTTGCTTGCTTTTGCAACCTGATAAAAAGGCTGTACTCGCCATTGATCCGTGGGAAGGTATAAGCCGCGTAGCTGTGAGTTATCAGTTACCACAGAAATGTCGAAGTGCAATGCATGTAAAGTAAAGAACGCCAAATGTTTTTTTGGGCTGCATGATGATATATGGACCGTTGTCTTCTACAAATTCTACAAACCTGATCCACAAAATAATAAATCACCTCCATTCAATGGGAGAAAAAGGCTATGCATAACATTATTTTTCCCTTGATAAAGGCAGCATGCTTCATTCGATTGCATTTTTCCTACATCTTTCAAAATCTAAGAACGAACAAAATTGGTAAGGACGTCAACAGAAAGACGCTCACAGTTGCAAGTTTGCAACTAGATCCACATAATTTCTGATTTCTACAGAATGCGTTGCCACAGTTTGAGAAGTGAGAGGTAATTTCTACAAGGAATCCTGGGCTATTGCGTTCCTATTGCAGCATGATGGGGAGGTGCTCTGAGAAGTCCTGTATCGGCGATAACTCCTGGAGCTCGTAGGCTGCCTGGAAGCAGTCGGCCGCTTCAAGCAGTGATCCTTCAGATTTCAAGACGAGTCCAAGGCGCAGCCAGGCTTGATGGCTTGTGGGTTCCAAACGGAGGGCATTACGGAGGAACGTTCTTGCGATCGACAATGATTTCCCTCCAAGAATGGTTAGAATTCCTGCCATGCAAACCATGCTTGGGACATAGTCTGGATCGATGGAGAGGGAGAGCGAGAACGCCGTCAGGGCTTCCT
CDS Sequence
  • >LOC_Os03g10340.1
    ATGGCCGTAGGTAAGAACAAGCGCATCTCCAAGGGGAAGAAGGGATCCAAGAAGAAGACCGTCGATCCCTTTGCTAAGAAGGACTGGTATGATATCAAGGCCCCGTCGGTGTTCAATGTGCGGAACATCGGCAAGACCCTCGTGTCCAGGACACAGGGTACAAAGATTGCTTCTGAGGGCCTAAAGCATAGAGTGTTTGAGGTCTCCTTAGCTGATCTCCAGAACGATGAGGATCAGGCGTACAGGAAGATCAGACTTCGTGCTGAGGATGTGCAAGGGAAGAACGTGCTCACCAACTTTTGGGGCATGTCGTTTACTACCGATAAGCTCAGATCACTTGTTAAGAAGTGGCAGACACTGATTGAGGCTCATGTGGATGTCAAAACCACAGACGGCTACATGCTGCGTCTGTTCTGTATCGGCTTCACCAAGCGGCGGCCTAACCAGGTGAAGAGGACTTGCTATGCTCAAGCGAGTCAGATCAGACAGATTCGTCGCAAGATGGTTGAAATCATGGCCAACCAGGCTTCAAGCTGTGATTTGAAAGAGCTAGTTTCAAAGTTCATTCCTGAAGTTATTGGGAAGGAAATTGAGAAGGCGACATCCAGTATTTTCCCCCTTCAGAATGTGTTTGTCCGCAAAGTGAAGATTCTAAAGGCCCCGAAATTTGACCTGGGGAAGCTCATGGAGGTGCATGGTGACTATGCCAAGGAGGACATCGGTACGAAGCTTGATAGGCCTGCAGAAGATGAGGCCATGGCCGGGCAGGAGGTCGCTGCTGCCGAGTAG
Protein Sequence
  • >LOC_Os03g10340.1
    MAVGKNKRISKGKKGSKKKTVDPFAKKDWYDIKAPSVFNVRNIGKTLVSRTQGTKIASEGLKHRVFEVSLADLQNDEDQAYRKIRLRAEDVQGKNVLTNFWGMSFTTDKLRSLVKKWQTLIEAHVDVKTTDGYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIMANQASSCDLKELVSKFIPEVIGKEIEKATSSIFPLQNVFVRKVKILKAPKFDLGKLMEVHGDYAKEDIGTKLDRPAEDEAMAGQEVAAAE*