Information report for NAL21
Gene Details
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Functional Descriptions
- Microscopic observation revealed defects in the vascular system and reduced epidermal cell size and number in the NAL21 leaf blade.
- Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of NAL21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs.
- The NAL21 mutant showed aberrant auxin responses in which multiple auxin response factors (ARFs) harboring upstream open reading frames (uORFs) in their 5’-untranslated region (5’-UTR) were repressed at the translational level.
- Ribosome-targeting antibiotics resistance assay and ribosome profiling showed a significant reduction in the free 40S ribosome subunit in the NAL21 mutant.
- Map-based cloning revealed that NAL21 encodes a ribosomal small subunit protein RPS3A.
Functional Keywords
Literature and News
Gene Resources
- UniProt: P49397
- EMBL: AC115687, AK069251, AK104243
- AlphaFoldDB: P49397
- EnsemblPlants: Os03t0200500-01
- Gramene: Os03t0200500-01
- KEGG: dosa:Os03g0200500
- Orthologous matrix: MHNDESD
Sequences
cDNA Sequence
- >LOC_Os03g10340.1
GGAGATAGGGTTTCTCCTCGCCGCTTCCACCTCTCTCGCGCCGCCGCCGCCGCCGAGCGCCTGGAGCAGCGTCAGCCATGGCCGTAGGTAAGAACAAGCGCATCTCCAAGGGGAAGAAGGGATCCAAGAAGAAGACCGTCGATCCCTTTGCTAAGAAGGACTGGTATGATATCAAGGCCCCGTCGGTGTTCAATGTGCGGAACATCGGCAAGACCCTCGTGTCCAGGACACAGGGTACAAAGATTGCTTCTGAGGGCCTAAAGCATAGAGTGTTTGAGGTCTCCTTAGCTGATCTCCAGAACGATGAGGATCAGGCGTACAGGAAGATCAGACTTCGTGCTGAGGATGTGCAAGGGAAGAACGTGCTCACCAACTTTTGGGGCATGTCGTTTACTACCGATAAGCTCAGATCACTTGTTAAGAAGTGGCAGACACTGATTGAGGCTCATGTGGATGTCAAAACCACAGACGGCTACATGCTGCGTCTGTTCTGTATCGGCTTCACCAAGCGGCGGCCTAACCAGGTGAAGAGGACTTGCTATGCTCAAGCGAGTCAGATCAGACAGATTCGTCGCAAGATGGTTGAAATCATGGCCAACCAGGCTTCAAGCTGTGATTTGAAAGAGCTAGTTTCAAAGTTCATTCCTGAAGTTATTGGGAAGGAAATTGAGAAGGCGACATCCAGTATTTTCCCCCTTCAGAATGTGTTTGTCCGCAAAGTGAAGATTCTAAAGGCCCCGAAATTTGACCTGGGGAAGCTCATGGAGGTGCATGGTGACTATGCCAAGGAGGACATCGGTACGAAGCTTGATAGGCCTGCAGAAGATGAGGCCATGGCCGGGCAGGAGGTCGCTGCTGCCGAGTAGAGATTATCACTTATTTTGCTGCCCGTTTATTTAGACAACAAGTAAACAGCTATCTAGTTGTGGATCGTGAACATCCTAGCCTGTGTCAATGGCAGATATTCTGAATAGTTTGTTGGGATTACTTGCCAGCTGCATATTTGCTTTCTGATGAGAAATGAATGTTTTTGAGTCTATGCGTTTGCTTTGTTTGGTCTTGATGATGAAGTTAAGAATTTTGAAGTAATTGTTTGGTCTGGAGTAGTTTTGATCCTGGCAGTCTTTAATTTCTTTGCTTGCTTTTGCAACCTGATAAAAAGGCTGTACTCGCCATTGATCCGTGGGAAGGTATAAGCCGCGTAGCTGTGAGTTATCAGTTACCACAGAAATGTCGAAGTGCAATGCATGTAAAGTAAAGAACGCCAAATGTTTTTTTGGGCTGCATGATGATATATGGACCGTTGTCTTCTACAAATTCTACAAACCTGATCCACAAAATAATAAATCACCTCCATTCAATGGGAGAAAAAGGCTATGCATAACATTATTTTTCCCTTGATAAAGGCAGCATGCTTCATTCGATTGCATTTTTCCTACATCTTTCAAAATCTAAGAACGAACAAAATTGGTAAGGACGTCAACAGAAAGACGCTCACAGTTGCAAGTTTGCAACTAGATCCACATAATTTCTGATTTCTACAGAATGCGTTGCCACAGTTTGAGAAGTGAGAGGTAATTTCTACAAGGAATCCTGGGCTATTGCGTTCCTATTGCAGCATGATGGGGAGGTGCTCTGAGAAGTCCTGTATCGGCGATAACTCCTGGAGCTCGTAGGCTGCCTGGAAGCAGTCGGCCGCTTCAAGCAGTGATCCTTCAGATTTCAAGACGAGTCCAAGGCGCAGCCAGGCTTGATGGCTTGTGGGTTCCAAACGGAGGGCATTACGGAGGAACGTTCTTGCGATCGACAATGATTTCCCTCCAAGAATGGTTAGAATTCCTGCCATGCAAACCATGCTTGGGACATAGTCTGGATCGATGGAGAGGGAGAGCGAGAACGCCGTCAGGGCTTCCT
CDS Sequence
- >LOC_Os03g10340.1
ATGGCCGTAGGTAAGAACAAGCGCATCTCCAAGGGGAAGAAGGGATCCAAGAAGAAGACCGTCGATCCCTTTGCTAAGAAGGACTGGTATGATATCAAGGCCCCGTCGGTGTTCAATGTGCGGAACATCGGCAAGACCCTCGTGTCCAGGACACAGGGTACAAAGATTGCTTCTGAGGGCCTAAAGCATAGAGTGTTTGAGGTCTCCTTAGCTGATCTCCAGAACGATGAGGATCAGGCGTACAGGAAGATCAGACTTCGTGCTGAGGATGTGCAAGGGAAGAACGTGCTCACCAACTTTTGGGGCATGTCGTTTACTACCGATAAGCTCAGATCACTTGTTAAGAAGTGGCAGACACTGATTGAGGCTCATGTGGATGTCAAAACCACAGACGGCTACATGCTGCGTCTGTTCTGTATCGGCTTCACCAAGCGGCGGCCTAACCAGGTGAAGAGGACTTGCTATGCTCAAGCGAGTCAGATCAGACAGATTCGTCGCAAGATGGTTGAAATCATGGCCAACCAGGCTTCAAGCTGTGATTTGAAAGAGCTAGTTTCAAAGTTCATTCCTGAAGTTATTGGGAAGGAAATTGAGAAGGCGACATCCAGTATTTTCCCCCTTCAGAATGTGTTTGTCCGCAAAGTGAAGATTCTAAAGGCCCCGAAATTTGACCTGGGGAAGCTCATGGAGGTGCATGGTGACTATGCCAAGGAGGACATCGGTACGAAGCTTGATAGGCCTGCAGAAGATGAGGCCATGGCCGGGCAGGAGGTCGCTGCTGCCGAGTAG
Protein Sequence
- >LOC_Os03g10340.1
MAVGKNKRISKGKKGSKKKTVDPFAKKDWYDIKAPSVFNVRNIGKTLVSRTQGTKIASEGLKHRVFEVSLADLQNDEDQAYRKIRLRAEDVQGKNVLTNFWGMSFTTDKLRSLVKKWQTLIEAHVDVKTTDGYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIMANQASSCDLKELVSKFIPEVIGKEIEKATSSIFPLQNVFVRKVKILKAPKFDLGKLMEVHGDYAKEDIGTKLDRPAEDEAMAGQEVAAAE*