Information report for OsRAF;OsLG3
Gene Details
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Functional Descriptions
- OsRAF is an ethylene responsive and root abundant factor gene of rice.
- Here, we report OsRAF (a Root Abundant Factor gene in Oryza sativa), a new member of the rice ERF group, expressed more abundantly in roots than in other organs of rice at the transcriptional level.
- OsLG3 contributing to rice grain length and yield was mined by Ho-LAMap.
- Phylogenetic analysis showed that OsLG3 might have much potential value for improvement of grain length in japonica breeding.
- The results demonstrated that Ho-LAMap is a potential approach for gene discovery and OsLG3 is a promising gene to be utilized in genomic assisted breeding for rice cultivar improvement.
- Here, we report that the ERF family transcription factor OsLG3 positively regulates drought tolerance in rice.
- In our previous work, we found that OsLG3 has a positive effect on rice grain length without affecting grain quality.
- Collectively, our findings reveal that natural variation in OsLG3 contributes to rice drought tolerance and that the elite allele of OsLG3 is a promising genetic resource for the development of drought-tolerant rice varieties.
- Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging.
- Phylogenetic analysis indicated that the tolerant allele of OsLG3 may improve drought tolerance in cultivated japonica rice.
- Introgression lines and complementation transgenic lines containing the elite allele of OsLG3IRAT109 showed increased drought tolerance, demonstrating that natural variation in OsLG3 contributes to drought tolerance in rice.
- By performing candidate gene association analysis, we found that natural variation in the promoter of OsLG3 is associated with tolerance to osmotic stress in germinating rice seeds.
- Overexpression of OsLG3 significantly improved the tolerance of rice plants to simulated drought, whereas suppression of OsLG3 resulted in greater susceptibility.
- Further investigation suggested that OsLG3 plays a positive role in drought-stress tolerance in rice by inducing reactive oxygen species scavenging.
- Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice.
- Compared with wild type, transgenic lines overexpressing OsSTAP1 exhibited increased tolerance to salt stress with higher SOD, POD, and CAT activities, and lower Na+/K+ ratios in the shoots.
- Conclusion: This study suggests that OsSTAP1 functions as an AP2/ERF transcriptional activator, and plays a positive role in salt tolerance by decreasing the Na+/K+ ratio and maintaining cellular redox homeostasis.
- OsSTAP1 is localized to the nucleus and acts as a transcriptional activator in plant cells.
Functional Keywords
- ethylene , root , grain , grain-length , yield , breeding , transcription-factor , development , drought , tolerance , drought-tolerance , stress , quality , stress-tolerance , reactive-oxygen-species , grain-quality , salt , salt-tolerance , salt-stress , homeostasis , nucleus , transcriptional-activator , redox-homeostasis
Literature and News
- OsRAF is an ethylene responsive and root abundant factor gene of rice . DOI: 10.1007/s10725-007-9228-5
- OsLG3 contributing to rice grain length and yield was mined by Ho-LAMap . DOI: 10.1186/s12915-017-0365-7 ; PMID: 28385155
- Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging . DOI: 10.1104/pp.17.01492 ; PMID: 30068540
- Bin-based genome-wide association studies reveal superior alleles for improvement of appearance quality using a 4-way MAGIC population in rice . DOI: 10.1016/j.jare.2020.08.001 ; PMID: 33364055
- Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice . DOI: 10.1186/s12284-020-00405-4 ; PMID: 32705427
Gene Resources
- UniProt: Q10QU5
- EMBL: AK060527, AK101949, AP014959
- AlphaFoldDB: Q10QU5
- EnsemblPlants: Os03t0183000-01, Os03t0183000-02
- Gramene: Os03t0183000-01, Os03t0183000-02
- KEGG: osa:4331845
- Orthologous matrix: MFPYESM
- InterPro: IPR001471, IPR016177, IPR036955
- PANTHER: PTHR31190, PTHR31190:SF194
- SUPFAM: SSF54171
- PROSITE: PS51032
- Gene3D: 3.30.730.10
- OrthoDB: Q10QU5
- SWISS-MODEL: Q10QU5
- Conserved Domain Database: cd00018
- eggNOG: ENOG502QV26
Sequences
cDNA Sequence
- >LOC_Os03g08470.3
GGTTACAAGAACAAAGGCATTCGCAACACACACTTGAAGAAAAAAAACACGACGAACACGTTAAAAAAAGGTCGAAGAGAAGTGGGAGACCCAAACCGCGAACAATGTGTGGAGGCGCCATCCTCGCCGAGTTCATCCCGGCGCCGTCGCGCGCCGCGGCGGCGACCAAGCGGGTGACCGCCAGCCACCTGTGGCCGGCCGGCTCCAAGAACGCCGCCCGCGGCAAGAGCAAGAGCAAGAGGCAGCAGAGGAGCTTCGCCGACGTCGACGACTTCGAGGCCGCCTTCGAGCAGTTCGACGATGACTCCGACTTCGACGACGCGGAGGAAGAAGACGAAGGACACTTCGTGTTCGCGTCCAAATCTCGTGTCGTCGCCGGGCACGACGGGCGCGCGGCGGCGAGGGCGGCGAGCAAGAAGAAGCGGGGGCGGCACTTCCGAGGCATCCGGCAGCGGCCATGGGGGAAGTGGGCGGCGGAGATCCGCGACCCGCACAAGGGCACGCGCGTCTGGCTCGGCACGTTCAACACCCCGGAGGAGGCCGCACGCGCCTACGACGTCGAGGCGCGCCGCCTCCGCGGCAGCAAGGCCAAGGTCAACTTCCCCGCCACGCCCGCCGCCGCGCGCCCACGCCGCGGCAACACGAGAGCCACCGCCGTGCCACCGCCGGCGACAGCACCCGCCGCCGCCCCGCCGCGCGGACTGAAGCGAGAATTCTCGCCGCCTGCTGAGACCGCGCTACCTTTCTTCACCAACGGCTTCGTCGACCTGACGACCGCCGCGGCGCCGCCACCGGCCATGATGATGACGAGCTCCTTCACCGACAGCGTCGCCACGTCGGAGTCCGGCGGGAGCCCCGCCAAGAAGGCGAGGTCCGACGACGTCGACTCGTCCGAGGGCAGCGTCGGCGGCGGCAGCGACACGCTGGGTTTCACCGACGAGCTGGAGTTCGACCCGTTCATGCTGTTCCAGCTCCCCTACTCCGACGGCTACGAGTCCATCGACAGCCTCTTCGCCGCCGGCGACGCCAACAGCGCGAACACCGACATGAACGCCGGCGTCAACCTGTGGAGCTTCGACGACTTCCCAATCGACGGCGCCCTTTTCTGATGTACTCCTCCATTGATGCAGTTTGTGCACTCAATTATACCATGCCATTTCTTGGTTCAGGTTAATCACGTCGTCGTTGATGAATGTTTAACCGGAGGATGATGGGGTGCAGCTGATATCATGGCTTGCTTGATTACCGAATTATATTGCGGTGTTTGTGTTTGTCAATTAGATTCTGAATCTGTACTACTGCCGATCTTATGATCAAAACTATATATTAAGTTTATGTACTCTTAATTTGTGGGCTACTTTTACGGGGTCTTCGTACTGCTGGTCAAATAGTCCTATGAAATCGATCATGTCGGCAATATCGTCGTCAATTATCGTGGTCGTGGTTGTTAATGATGTCAAATAAGTCTATGAAAACCTGGTCCTCTTGGTGTTTATGTACCACTGATCGATCCATCATTTGATCTCTGTCATGTTGAGATGGATCGTGTAAGAATATCATAATTTGCTGGATTCAGTCCAGTCGTAATGTATTTTGGTTTGTACAACTGCAATTTTGCTTTGTTTGGATTATAACTGCTGAAAAAATAGAGGGAGAGAAAGACAGGTTTTTTTTTA - >LOC_Os03g08470.2
GGTTACAAGAACAAAGGCATTCGCAACACACACTTGAAGAAAAAAAACACGACGAACACGTTAAAAAAAGGTCGAAGAGAAGTGGGAGACCCAAACCGCGAACAATGTGTGGAGGCGCCATCCTCGCCGAGTTCATCCCGGCGCCGTCGCGCGCCGCGGCGGCGACCAAGCGGGTGACCGCCAGCCACCTGTGGCCGGCCGGCTCCAAGAACGCCGCCCGCGGCAAGAGCAAGAGCAAGAGGCAGCAGAGGAGCTTCGCCGACGTCGACGACTTCGAGGCCGCCTTCGAGCAGTTCGACGATGACTCCGACTTCGACGACGCGGAGGAAGAAGACGAAGGACACTTCGTGTTCGCGTCCAAATCTCGTGTCGTCGCCGGGCACGACGGGCGCGCGGCGGCGAGGGCGGCGAGCAAGAAGAAGCGGGGGCGGCACTTCCGAGGCATCCGGCAGCGGCCATGGGGGAAGTGGGCGGCGGAGATCCGCGACCCGCACAAGGGCACGCGCGTCTGGCTCGGCACGTTCAACACCCCGGAGGAGGCCGCACGCGCCTACGACGTCGAGGCGCGCCGCCTCCGCGGCAGCAAGGCCAAGGTCAACTTCCCCGCCACGCCCGCCGCCGCGCGCCCACGCCGCGGCAACACGAGAGCCACCGCCGTGCCACCGCCGGCGACAGCACCCGCCGCCGCCCCGCCGCGCGGACTGAAGCGAGAATTCTCGCCGCCTGCTGAGACCGCGCTACCTTTCTTCACCAACGGCTTCGTCGACCTGACGACCGCCGCGGCGCCGCCACCGGCCATGATGATGACGAGCTCCTTCACCGACAGCGTCGCCACGTCGGAGTCCGGCGGGAGCCCCGCCAAGAAGGCGAGGTCCGACGACGTCGACTCGTCCGAGGGCAGCGTCGGCGGCGGCAGCGACACGCTGGGTTTCACCGACGAGCTGGAGTTCGACCCGTTCATGCTGTTCCAGCTCCCCTACTCCGACGGCTACGAGTCCATCGACAGCCTCTTCGCCGCCGGCGACGCCAACAGCGCGAACACCGACATGAACGCCGGCGTCAACCTGTGGAGCTTCGACGACTTCCCAATCGACGGCGCCCTTTTCTGATGTACTCCTCCATTGATGCAGTTTGTGCACTCAATTATACCATGCCATTTCTTGGTTCAGGTTAATCACGTCGTCGTTGATGAATGTTTAACCGGAGGATGATGGGGTGCAGCTGATATCATGGCTTGCTTGATTACCGAATTATATTGCGGTGTTTGTGTTTGTCAATTAGATTCTGAATCTGTACTACTGCCGATCTTATGATCAAAACTATATATTAAGTTTATGTACTCTTAATTTGTGGGCTACTTTTACGGGGTCTTCGTACTGCTGGTCAAATAGTCCTATGAAATCGATCATGTCGGCAATATCGTCGTCAATTATCGTGGTCGTGGTTGTTAATGATGTCAAATAAGTCTATGAAAACCTGGTCCTCTTGGTGTTTATGTACCACTGATCGATCCATCATTTGATCTCTGTCATGTTGAGATGGATCGTGTAAGAATATCATAATTTGCTGGATTCAGTCCAGTCGTAATGTATTTTGGTTTGTACAACTGCAATTTTGCTTTGTTTGGATTATAACTGCTGAAAAAATAGAGGGAGAGAAAGACAGGTTTTTTTTTA - >LOC_Os03g08470.1
GGTTACAAGAACAAAGGCATTCGCAACACACACTTGAAGAAAAAAAACACGACGAACACGTTAAAAAAAGGTCGAAGAGAAGTGGGAGACCCAAACCGCGAACAATGTGTGGAGGCGCCATCCTCGCCGAGTTCATCCCGGCGCCGTCGCGCGCCGCGGCGGCGACCAAGCGGGTGACCGCCAGCCACCTGTGGCCGGCCGGCTCCAAGAACGCCGCCCGCGGCAAGAGCAAGAGCAAGAGGCAGCAGAGGAGCTTCGCCGACGTCGACGACTTCGAGGCCGCCTTCGAGCAGTTCGACGATGACTCCGACTTCGACGACGCGGAGGAAGAAGACGAAGGACACTTCGTGTTCGCGTCCAAATCTCGTGTCGTCGCCGGGCACGACGGGCGCGCGGCGGCGAGGGCGGCGAGCAAGAAGAAGCGGGGGCGGCACTTCCGAGGCATCCGGCAGCGGCCATGGGGGAAGTGGGCGGCGGAGATCCGCGACCCGCACAAGGGCACGCGCGTCTGGCTCGGCACGTTCAACACCCCGGAGGAGGCCGCACGCGCCTACGACGTCGAGGCGCGCCGCCTCCGCGGCAGCAAGGCCAAGGTCAACTTCCCCGCCACGCCCGCCGCCGCGCGCCCACGCCGCGGCAACACGAGAGCCACCGCCGTGCCACCGCCGGCGACAGCACCCGCCGCCGCCCCGCCGCGCGGACTGAAGCGAGAATTCTCGCCGCCTGCTGAGACCGCGCTACCTTTCTTCACCAACGGCTTCGTCGACCTGACGACCGCCGCGGCGCCGCCACCGGCCATGATGATGACGAGCTCCTTCACCGACAGCGTCGCCACGTCGGAGTCCGGCGGGAGCCCCGCCAAGAAGGCGAGGTCCGACGACGTCGACTCGTCCGAGGGCAGCGTCGGCGGCGGCAGCGACACGCTGGGTTTCACCGACGAGCTGGAGTTCGACCCGTTCATGCTGTTCCAGCTCCCCTACTCCGACGGCTACGAGTCCATCGACAGCCTCTTCGCCGCCGGCGACGCCAACAGCGCGAACACCGACATGAACGCCGGCGTCAACCTGTGGAGCTTCGACGACTTCCCAATCGACGGCGCCCTTTTCTGATGTACTCCTCCATTGATGCAGTTTGTGCACTCAATTATACCATGCCATTTCTTGGTTCAGGTTAATCACGTCGTCGTTGATGAATGTTTAACCGGAGGATGATGGGGTGCAGCTGATATCATGGCTTGCTTGATTACCGAATTATATTGCGGTGTTTGTGTTTGTCAATTAGATTCTGAATCTGTACTACTGCCGATCTTATGATCAAAACTATATATTAAGTTTATGTACTCTTAATTTGTGGGCTACTTTTACGGGGTCTTCGTACTGCTGGTCAAATAGTCCTATGAAATCGATCATGTCGGCAATATCGTCGTCAATTATCGTGGTCGTGGTTGTTAATGATGTCAAATAAGTCTATGAAAACCTGGTCCTCTTGGTGTTTATGTACCACTGATCGATCCATCATTTGATCTCTGTCATGTTGAGATGGATCGTGTAAGAATATCATAATTTGCTGGATTCAGTCCAGTCGTAATGTATTTTGGTTTGTACAACTGCAATTTTGCTTTGTTTGGATTATAACTGCTGAAAAAATAGAGGGAGAGAAAGACAGGTTTTTTTTTA
CDS Sequence
- >LOC_Os03g08470.3
ATGTGTGGAGGCGCCATCCTCGCCGAGTTCATCCCGGCGCCGTCGCGCGCCGCGGCGGCGACCAAGCGGGTGACCGCCAGCCACCTGTGGCCGGCCGGCTCCAAGAACGCCGCCCGCGGCAAGAGCAAGAGCAAGAGGCAGCAGAGGAGCTTCGCCGACGTCGACGACTTCGAGGCCGCCTTCGAGCAGTTCGACGATGACTCCGACTTCGACGACGCGGAGGAAGAAGACGAAGGACACTTCGTGTTCGCGTCCAAATCTCGTGTCGTCGCCGGGCACGACGGGCGCGCGGCGGCGAGGGCGGCGAGCAAGAAGAAGCGGGGGCGGCACTTCCGAGGCATCCGGCAGCGGCCATGGGGGAAGTGGGCGGCGGAGATCCGCGACCCGCACAAGGGCACGCGCGTCTGGCTCGGCACGTTCAACACCCCGGAGGAGGCCGCACGCGCCTACGACGTCGAGGCGCGCCGCCTCCGCGGCAGCAAGGCCAAGGTCAACTTCCCCGCCACGCCCGCCGCCGCGCGCCCACGCCGCGGCAACACGAGAGCCACCGCCGTGCCACCGCCGGCGACAGCACCCGCCGCCGCCCCGCCGCGCGGACTGAAGCGAGAATTCTCGCCGCCTGCTGAGACCGCGCTACCTTTCTTCACCAACGGCTTCGTCGACCTGACGACCGCCGCGGCGCCGCCACCGGCCATGATGATGACGAGCTCCTTCACCGACAGCGTCGCCACGTCGGAGTCCGGCGGGAGCCCCGCCAAGAAGGCGAGGTCCGACGACGTCGACTCGTCCGAGGGCAGCGTCGGCGGCGGCAGCGACACGCTGGGTTTCACCGACGAGCTGGAGTTCGACCCGTTCATGCTGTTCCAGCTCCCCTACTCCGACGGCTACGAGTCCATCGACAGCCTCTTCGCCGCCGGCGACGCCAACAGCGCGAACACCGACATGAACGCCGGCGTCAACCTGTGGAGCTTCGACGACTTCCCAATCGACGGCGCCCTTTTCTGA - >LOC_Os03g08470.2
ATGTGTGGAGGCGCCATCCTCGCCGAGTTCATCCCGGCGCCGTCGCGCGCCGCGGCGGCGACCAAGCGGGTGACCGCCAGCCACCTGTGGCCGGCCGGCTCCAAGAACGCCGCCCGCGGCAAGAGCAAGAGCAAGAGGCAGCAGAGGAGCTTCGCCGACGTCGACGACTTCGAGGCCGCCTTCGAGCAGTTCGACGATGACTCCGACTTCGACGACGCGGAGGAAGAAGACGAAGGACACTTCGTGTTCGCGTCCAAATCTCGTGTCGTCGCCGGGCACGACGGGCGCGCGGCGGCGAGGGCGGCGAGCAAGAAGAAGCGGGGGCGGCACTTCCGAGGCATCCGGCAGCGGCCATGGGGGAAGTGGGCGGCGGAGATCCGCGACCCGCACAAGGGCACGCGCGTCTGGCTCGGCACGTTCAACACCCCGGAGGAGGCCGCACGCGCCTACGACGTCGAGGCGCGCCGCCTCCGCGGCAGCAAGGCCAAGGTCAACTTCCCCGCCACGCCCGCCGCCGCGCGCCCACGCCGCGGCAACACGAGAGCCACCGCCGTGCCACCGCCGGCGACAGCACCCGCCGCCGCCCCGCCGCGCGGACTGAAGCGAGAATTCTCGCCGCCTGCTGAGACCGCGCTACCTTTCTTCACCAACGGCTTCGTCGACCTGACGACCGCCGCGGCGCCGCCACCGGCCATGATGATGACGAGCTCCTTCACCGACAGCGTCGCCACGTCGGAGTCCGGCGGGAGCCCCGCCAAGAAGGCGAGGTCCGACGACGTCGACTCGTCCGAGGGCAGCGTCGGCGGCGGCAGCGACACGCTGGGTTTCACCGACGAGCTGGAGTTCGACCCGTTCATGCTGTTCCAGCTCCCCTACTCCGACGGCTACGAGTCCATCGACAGCCTCTTCGCCGCCGGCGACGCCAACAGCGCGAACACCGACATGAACGCCGGCGTCAACCTGTGGAGCTTCGACGACTTCCCAATCGACGGCGCCCTTTTCTGA - >LOC_Os03g08470.1
ATGTGTGGAGGCGCCATCCTCGCCGAGTTCATCCCGGCGCCGTCGCGCGCCGCGGCGGCGACCAAGCGGGTGACCGCCAGCCACCTGTGGCCGGCCGGCTCCAAGAACGCCGCCCGCGGCAAGAGCAAGAGCAAGAGGCAGCAGAGGAGCTTCGCCGACGTCGACGACTTCGAGGCCGCCTTCGAGCAGTTCGACGATGACTCCGACTTCGACGACGCGGAGGAAGAAGACGAAGGACACTTCGTGTTCGCGTCCAAATCTCGTGTCGTCGCCGGGCACGACGGGCGCGCGGCGGCGAGGGCGGCGAGCAAGAAGAAGCGGGGGCGGCACTTCCGAGGCATCCGGCAGCGGCCATGGGGGAAGTGGGCGGCGGAGATCCGCGACCCGCACAAGGGCACGCGCGTCTGGCTCGGCACGTTCAACACCCCGGAGGAGGCCGCACGCGCCTACGACGTCGAGGCGCGCCGCCTCCGCGGCAGCAAGGCCAAGGTCAACTTCCCCGCCACGCCCGCCGCCGCGCGCCCACGCCGCGGCAACACGAGAGCCACCGCCGTGCCACCGCCGGCGACAGCACCCGCCGCCGCCCCGCCGCGCGGACTGAAGCGAGAATTCTCGCCGCCTGCTGAGACCGCGCTACCTTTCTTCACCAACGGCTTCGTCGACCTGACGACCGCCGCGGCGCCGCCACCGGCCATGATGATGACGAGCTCCTTCACCGACAGCGTCGCCACGTCGGAGTCCGGCGGGAGCCCCGCCAAGAAGGCGAGGTCCGACGACGTCGACTCGTCCGAGGGCAGCGTCGGCGGCGGCAGCGACACGCTGGGTTTCACCGACGAGCTGGAGTTCGACCCGTTCATGCTGTTCCAGCTCCCCTACTCCGACGGCTACGAGTCCATCGACAGCCTCTTCGCCGCCGGCGACGCCAACAGCGCGAACACCGACATGAACGCCGGCGTCAACCTGTGGAGCTTCGACGACTTCCCAATCGACGGCGCCCTTTTCTGA
Protein Sequence
- >LOC_Os03g08470.3
MCGGAILAEFIPAPSRAAAATKRVTASHLWPAGSKNAARGKSKSKRQQRSFADVDDFEAAFEQFDDDSDFDDAEEEDEGHFVFASKSRVVAGHDGRAAARAASKKKRGRHFRGIRQRPWGKWAAEIRDPHKGTRVWLGTFNTPEEAARAYDVEARRLRGSKAKVNFPATPAAARPRRGNTRATAVPPPATAPAAAPPRGLKREFSPPAETALPFFTNGFVDLTTAAAPPPAMMMTSSFTDSVATSESGGSPAKKARSDDVDSSEGSVGGGSDTLGFTDELEFDPFMLFQLPYSDGYESIDSLFAAGDANSANTDMNAGVNLWSFDDFPIDGALF* - >LOC_Os03g08470.2
MCGGAILAEFIPAPSRAAAATKRVTASHLWPAGSKNAARGKSKSKRQQRSFADVDDFEAAFEQFDDDSDFDDAEEEDEGHFVFASKSRVVAGHDGRAAARAASKKKRGRHFRGIRQRPWGKWAAEIRDPHKGTRVWLGTFNTPEEAARAYDVEARRLRGSKAKVNFPATPAAARPRRGNTRATAVPPPATAPAAAPPRGLKREFSPPAETALPFFTNGFVDLTTAAAPPPAMMMTSSFTDSVATSESGGSPAKKARSDDVDSSEGSVGGGSDTLGFTDELEFDPFMLFQLPYSDGYESIDSLFAAGDANSANTDMNAGVNLWSFDDFPIDGALF* - >LOC_Os03g08470.1
MCGGAILAEFIPAPSRAAAATKRVTASHLWPAGSKNAARGKSKSKRQQRSFADVDDFEAAFEQFDDDSDFDDAEEEDEGHFVFASKSRVVAGHDGRAAARAASKKKRGRHFRGIRQRPWGKWAAEIRDPHKGTRVWLGTFNTPEEAARAYDVEARRLRGSKAKVNFPATPAAARPRRGNTRATAVPPPATAPAAAPPRGLKREFSPPAETALPFFTNGFVDLTTAAAPPPAMMMTSSFTDSVATSESGGSPAKKARSDDVDSSEGSVGGGSDTLGFTDELEFDPFMLFQLPYSDGYESIDSLFAAGDANSANTDMNAGVNLWSFDDFPIDGALF*