Gene Details:

• MSU gene ID: LOC_Os03g08470
• RAPdb gene ID: Os03g0183000
• Gene Symbol: OsRAF OsLG3 OsSTAP1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• OsRAF is an ethylene responsive and root abundant factor gene of rice.
• Here, we report OsRAF (a Root Abundant Factor gene in Oryza sativa), a new member of the rice ERF group, expressed more abundantly in roots than in other organs of rice at the transcriptional level.
• OsLG3 contributing to rice grain length and yield was mined by Ho-LAMap.
• Phylogenetic analysis showed that OsLG3 might have much potential value for improvement of grain length in japonica breeding.
• The results demonstrated that Ho-LAMap is a potential approach for gene discovery and OsLG3 is a promising gene to be utilized in genomic assisted breeding for rice cultivar improvement.
• Here, we report that the ERF family transcription factor OsLG3 positively regulates drought tolerance in rice.
• In our previous work, we found that OsLG3 has a positive effect on rice grain length without affecting grain quality.
• Collectively, our findings reveal that natural variation in OsLG3 contributes to rice drought tolerance and that the elite allele of OsLG3 is a promising genetic resource for the development of drought-tolerant rice varieties.
• Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging.
• Phylogenetic analysis indicated that the tolerant allele of OsLG3 may improve drought tolerance in cultivated japonica rice.
• Introgression lines and complementation transgenic lines containing the elite allele of OsLG3IRAT109 showed increased drought tolerance, demonstrating that natural variation in OsLG3 contributes to drought tolerance in rice.
• By performing candidate gene association analysis, we found that natural variation in the promoter of OsLG3 is associated with tolerance to osmotic stress in germinating rice seeds.
• Overexpression of OsLG3 significantly improved the tolerance of rice plants to simulated drought, whereas suppression of OsLG3 resulted in greater susceptibility.
• Further investigation suggested that OsLG3 plays a positive role in drought-stress tolerance in rice by inducing reactive oxygen species scavenging.
• Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice.
• Compared with wild type, transgenic lines overexpressing OsSTAP1 exhibited increased tolerance to salt stress with higher SOD, POD, and CAT activities, and lower Na+/K+ ratios in the shoots.
• Conclusion: This study suggests that OsSTAP1 functions as an AP2/ERF transcriptional activator, and plays a positive role in salt tolerance by decreasing the Na+/K+ ratio and maintaining cellular redox homeostasis.
• OsSTAP1 is localized to the nucleus and acts as a transcriptional activator in plant cells.

Function-related keywords:

• ethylene ,  root ,  grain ,  grain-length ,  yield ,  breeding ,  transcription-factor ,  development ,  drought ,  tolerance ,  drought-tolerance ,  stress ,  quality ,  stress-tolerance ,  reactive-oxygen-species ,  grain-quality ,  salt ,  salt-tolerance ,  salt-stress ,  homeostasis ,  nucleus ,  transcriptional-activator ,  redox-homeostasis

Literature:

• OsRAF is an ethylene responsive and root abundant factor gene of rice .  DOI:  10.1007/s10725-007-9228-5
• OsLG3 contributing to rice grain length and yield was mined by Ho-LAMap .  DOI:  10.1186/s12915-017-0365-7 ;  PMID:  28385155
• Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging .  DOI:  10.1104/pp.17.01492 ;  PMID:  30068540
• Bin-based genome-wide association studies reveal superior alleles for improvement of appearance quality using a 4-way MAGIC population in rice .  DOI:  10.1016/j.jare.2020.08.001 ;  PMID:  33364055
• Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice .  DOI:  10.1186/s12284-020-00405-4 ;  PMID:  32705427

Related News:

Gene Resources:

• NCBI ID: EU837258
• UniProt accessions:

Sequences: