Gene Details:

Functional Descriptions:

  • The GUDK mutants interestingly showed a significant reduction in grain yield, both under normal well-watered conditions and under drought stress at the reproductive stage.
  • Loss-of-function GUDK mutant lines exhibit sensitivity to salinity, osmotic stress, and abscisic acid treatment at the seedling stage, and a reduction in photosynthesis and plant biomass under controlled drought stress at the vegetative stage.
  • Phosphoproteome profiling of the mutant followed by in vitro assays identified the transcription factor APETALA2/ETHYLENE RESPONSE FACTOR OsAP37 as a phosphorylation target of GUDK.
  • We propose that GUDK mediates drought stress signaling through phosphorylation and activation of OsAP37, resulting in transcriptional activation of stress-regulated genes, which impart tolerance and improve yield under drought.
  • Our transactivation assays confirmed that GUDK is required for activation of stress genes by OsAP37.
  • GUDK is induced under several stresses and its loss-of-function increased the sensitivity of rice seedlings to salinity, osmotic stress, and abscisic acid treatment.
  • In addition to reduced tolerance of GUDK mutant plants to drought stress at vegetative stage, a significant reduction in grain yield was observed under well-watered and drought stress conditions at reproductive stage.
  • Thus, our results suggest that GUDK has the potential to regulate grain yield both under favorable and unfavorable conditions.
  • Gene co-expression analysis supports the role of GUDK in regulating important biological processes both under control and stress conditions.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g08170.1
    ACACACCCCCAAACCAAAATTAACCAAACCCCCGCGCCGTTAACCCTCTGAGCTTGTGATGAATTAACGATCGTGACCGATCAAGTGAGCGATTAATAATAACCCAGCGGCACACAATTAAGCAATGCAAGAAGCCGTCGTCGTTAACACCGGCGGCCGGTGGGCACCAACCCGTATTTATATACACTTCTATCCGGCCAAAGCAGCGGGCGCGGCCGGGTAACCCAACCACACACGCACGACACGAAGAACGCCATGGCGAAGCAGGCGTGGAGCTCGCTGTTCGGGTGCTTCACCTCGCACCACGACGGCGGCGGCAAGAGGAAGAAAGGCGGCGGCGGCGGGAAGAAGGCGAAGAAGAAGGTGGCGGCGGCGGCGAGCCAGAAGCAGCGGAAGCTGCAGAGCCGGCTGTCGTTCTCCGACCTGTCGTTCGGAGGGATGGTGTCGCCGGAGGACCTGTCGCTGTCGCTGGCCGGGTCGAACCTCCACGTCTTCACCATCGCCGAGCTCCGCGCCGTCACCAGGGACTTCTCCATGACCAACTTCATCGGCGAGGGCGGCTTCGGCCCTGTCTACAAGGGCTACGTCGACGACAAGCTCAAGCCCGGCCTCCGCGCGCAGCCCGTCGCCGTCAAGCTCCTCGACCTCGAGGGCACACAGGGACACAACGAGTGGCTGACTGAAGTCATCTTTCTGGGGCAACTGAGGCATCCTCACCTCGTCAAACTGATCGGCTACTGCTACGAGGATGAACACAGACTTCTCGTTTACGAGTTCATGACGAGGGGCAGCTTGGAGAAGCATCTCTTCAAAAAGTACGCAGCGTCGTTGCCATGGTCAACACGGTTGAAGATCGCCATAGGTGCCGCGAAAGGGTTGGCCTTTCTCCATGAAGCCGAGAAGCCGGTCATCTACCGAGACTTCAAGACCTCCAACATCTTGCTAGACTCGGACTTCAAAGCCAAGCTCTCAGATTTCGGGCTAGCGAAGGACGGGCCAGAGGATGACGAGACGCATGTCTCGACACGTGTCATGGGCACACAGGGATACGCTGCACCAGAGTACATCATGACAGGGCATCTGACGGCGAAGAGCGACGTGTACGGCTTCGGCGTGGTGCTGCTGGAGCTGCTGTCGGGGAGGAAGTCGGTGGACAAGAGCCGGCCGGCGCGGGAGCAGAACCTGGTGGAGTGGGCCCGGCCGTACCTGACCGACGCGCGGCGGCTGGGCCGCGTCATGGACCGCAACCTCGCCGGCCAGTACCCGGCCAAGGCGGCGCAGAAGGCCGCCGCGCTGGCGCACCGCTGCGTCAGCCTCAACCCCAAGTCCCGCCCCCACATGTCCGCCGTCGTCGAGGCGCTCGAGCCGCTCCTCGCCCTCGACGACGACTGCCTCGTCGGCACCTTCGTCTACGTCGCGCCGCCCGACGACGTCGCCGCCAACGGCGACGGCAGCAGCAAGAGGCGAGCCGGCCGGAGGAGGTCTGACGGCGCCGCGGCGGCGGCGGCGGCCGATGGTGTGCAGCGGGAATGACTCGGTTTTGCTTGCGTTTGTCTGTACTCGTCGAGCTACGTGCTGATCAAGCGATGCCGGTAAATTAATCCGGATCAGTGAGTTGGCTCGTGGGTAAAAAATGATTGTGGATTTGAAGCATGTTATTATTTGTCTTAGGCTTGAATAGCAGAATAGGATTAGAGCGTGTAAATACGAGCATGTCAGACGTACATGAGCAAATTGAGCATGTAAACAGTTTGACAAAATATGGATGGTTTATCACTTCTCATGTTGGGAGGTTTCTCTAGTATCTTCCCAGCGCCGTGAAATATCTCTGTTGATAGACACTGTATGCTGTACGCGAGTATGTTATTACTGTTTGCTGAAAGACTGTAGGTTTTCTCCGTGTGCACTGTATTTAAATTTTGAGAAGCTGCAAGGATTTAGTAATGGTAATGTTGGTGGTGTACGGTGTGGGAATGCAATACAACTGTAACAATTGGACAGGGAGAA
CDS Sequence
  • >LOC_Os03g08170.1
    ATGGCGAAGCAGGCGTGGAGCTCGCTGTTCGGGTGCTTCACCTCGCACCACGACGGCGGCGGCAAGAGGAAGAAAGGCGGCGGCGGCGGGAAGAAGGCGAAGAAGAAGGTGGCGGCGGCGGCGAGCCAGAAGCAGCGGAAGCTGCAGAGCCGGCTGTCGTTCTCCGACCTGTCGTTCGGAGGGATGGTGTCGCCGGAGGACCTGTCGCTGTCGCTGGCCGGGTCGAACCTCCACGTCTTCACCATCGCCGAGCTCCGCGCCGTCACCAGGGACTTCTCCATGACCAACTTCATCGGCGAGGGCGGCTTCGGCCCTGTCTACAAGGGCTACGTCGACGACAAGCTCAAGCCCGGCCTCCGCGCGCAGCCCGTCGCCGTCAAGCTCCTCGACCTCGAGGGCACACAGGGACACAACGAGTGGCTGACTGAAGTCATCTTTCTGGGGCAACTGAGGCATCCTCACCTCGTCAAACTGATCGGCTACTGCTACGAGGATGAACACAGACTTCTCGTTTACGAGTTCATGACGAGGGGCAGCTTGGAGAAGCATCTCTTCAAAAAGTACGCAGCGTCGTTGCCATGGTCAACACGGTTGAAGATCGCCATAGGTGCCGCGAAAGGGTTGGCCTTTCTCCATGAAGCCGAGAAGCCGGTCATCTACCGAGACTTCAAGACCTCCAACATCTTGCTAGACTCGGACTTCAAAGCCAAGCTCTCAGATTTCGGGCTAGCGAAGGACGGGCCAGAGGATGACGAGACGCATGTCTCGACACGTGTCATGGGCACACAGGGATACGCTGCACCAGAGTACATCATGACAGGGCATCTGACGGCGAAGAGCGACGTGTACGGCTTCGGCGTGGTGCTGCTGGAGCTGCTGTCGGGGAGGAAGTCGGTGGACAAGAGCCGGCCGGCGCGGGAGCAGAACCTGGTGGAGTGGGCCCGGCCGTACCTGACCGACGCGCGGCGGCTGGGCCGCGTCATGGACCGCAACCTCGCCGGCCAGTACCCGGCCAAGGCGGCGCAGAAGGCCGCCGCGCTGGCGCACCGCTGCGTCAGCCTCAACCCCAAGTCCCGCCCCCACATGTCCGCCGTCGTCGAGGCGCTCGAGCCGCTCCTCGCCCTCGACGACGACTGCCTCGTCGGCACCTTCGTCTACGTCGCGCCGCCCGACGACGTCGCCGCCAACGGCGACGGCAGCAGCAAGAGGCGAGCCGGCCGGAGGAGGTCTGACGGCGCCGCGGCGGCGGCGGCGGCCGATGGTGTGCAGCGGGAATGA
Protein Sequence
  • >LOC_Os03g08170.1
    MAKQAWSSLFGCFTSHHDGGGKRKKGGGGGKKAKKKVAAAASQKQRKLQSRLSFSDLSFGGMVSPEDLSLSLAGSNLHVFTIAELRAVTRDFSMTNFIGEGGFGPVYKGYVDDKLKPGLRAQPVAVKLLDLEGTQGHNEWLTEVIFLGQLRHPHLVKLIGYCYEDEHRLLVYEFMTRGSLEKHLFKKYAASLPWSTRLKIAIGAAKGLAFLHEAEKPVIYRDFKTSNILLDSDFKAKLSDFGLAKDGPEDDETHVSTRVMGTQGYAAPEYIMTGHLTAKSDVYGFGVVLLELLSGRKSVDKSRPAREQNLVEWARPYLTDARRLGRVMDRNLAGQYPAKAAQKAAALAHRCVSLNPKSRPHMSAVVEALEPLLALDDDCLVGTFVYVAPPDDVAANGDGSSKRRAGRRRSDGAAAAAAADGVQRE*