Information report for CYP704B2
Gene Details
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Functional Descriptions
- Two genes CYP704B2 and WDA1, involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine, were downregulated in osabcg15 mutant, suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther locules.
- We report here the isolation and characterization of a rice (Oryza sativa) male sterile mutant, CYP704B2, which exhibits a swollen sporophytic tapetal layer, aborted pollen grains without detectable exine, and undeveloped anther cuticle.
- In addition, chemical composition analysis indicated that cutin monomers were hardly detectable in the CYP704B2 anthers.
- The CYP704B2 transcript is specifically detected in the tapetum and the microspore from stage 8 of anther development to stage 10.
- Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice.
Functional Keywords
- cuticle , anther , tapetal , pollen , sterile , tapetum , anther-development , cutin , grain , microspore
Literature and News
- The ATP-binding cassette transporter OsABCG15 is required for anther development and pollen fertility in rice . DOI: 10.1111/jipb.12053 ; PMID: 23570336
- Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice . DOI: 10.1105/tpc.109.070326 ; PMID: 20086189
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice . DOI: 10.1186/s12284-020-00451-y ; PMID: 33409767
Gene Resources
- UniProt: Q8S7V0
- EMBL: AP014959, DP000009
- AlphaFoldDB: Q8S7V0
- EnsemblPlants: Os03t0168600-01
- Gramene: Os03t0168600-01
- KEGG: osa:4331756
- Orthologous matrix: EMIPGPT
- InterPro: IPR001128, IPR002401, IPR036396
Sequences
cDNA Sequence
- >LOC_Os03g07250.1
ATAGCATCTCTTGTAGACTGAACCCTTCATGAAGAGCCCCATGGAGGAAGCTCATGCAATGCCAGTGACATCATTCTTCCCAGTAGCAGGAATCCACAAGCTCATAGCTATCTTCCTTGTTGTCCTCTCATGGATCTTGGTCCACAAGTGGAGCCTGAGGAACCAGAAAGGGCCAAGATCATGGCCAATCATCGGCGCGACAGTGGAGCAACTGAAGAACTACCACAGGATGCATGACTGGCTTGTCGAGTACTTGTCGAAGGACAGGACGGTGACCGTCGACATGCCTTTCACCTCCTACACCTACATTGCCGACCCGGTGAACGTCGAGCATGTCCTGAAGACCAACTTCACCAATTACCCCAAGGGTGAAGTGTACAGGTCTTACATGGATGTGCTGCTCGGTGATGGCATATTCAATGCCGACGGCGAGATGTGGAGGAAGCAAAGGAAGACGGCGAGCTTCGAGTTTGCCTCCAAGAACTTGAGAGACTTCAGCACTGTGGTGTTCAGGGAGTACTCCCTGAAGCTATCAAGCATTCTGAGCCAAGCATGCAAGGCCGGCAGAGTTGTAGACATGCAGGAATTGTTCATGAGGATGACACTGGACTCGATCTGCAAGGTCGGGTTTGGGGTTGAGATCGGGACGCTGTCACCTGATCTCCCGGAGAACAGCTTTGCCCAGGCATTCGACGCTGCCAACATCATCGTCACGCTGCGGTTCATCGATCCTCTGTGGCGTCTGAAGAAGTTCTTGCACGTCGGATCAGAGGCTCTCCTCGAGCAGAGCATGAAGCTGGTTGATGACTTCACCTACAGCGTGATCCGCCGCCGCAAGGCTGAGATCTTGCAGGCTCGAGCCAGCGGCAAGCAAGAGAAGATCAAGCACGACATACTGTCGCGGTTCATCGAGCTCGGGGAGGCCGGCGGCGACGAGGGGGGCGGCAGCTTCGGGGACGACAAGAGCCTCCGCGACGTGGTGCTCAACTTCGTGATCGCCGGGCGTGACACGACGGCGACGACGCTGTCGTGGTTCACGTACATGGCGATGACGCACCCGGCCGTCGCCGACAAGCTCCGGCGCGAGCTGGCCGCGTTCGAGGATGAGCGCGCGCGCGAGGAGGGCGTCGCGCTCGCCGACGCCGCCGGCGAGGCGTCGTTCGCGGCGCGCGTGGCGCAGTTCGCGTCGCTGCTGAGCTACGACGCGGTGGGGAAGCTGGTGTACCTGCACGCGTGCGTGACGGAGACGCTCCGCCTCTACCCGGCGGTGCCGCAGGACCCCAAGGGGATCGTGGAGGACGACGTGCTCCCCGACGGCACCAAGGTGCGCGCCGGCGGGATGGTGACGTACGTGCCCTACTCCATGGGGAGGATGGAGTACAACTGGGGCCCCGACGCGGCGAGCTTCCGGCCGGAGCGGTGGCTCAGCGGCGACGGCGGCGCGTTCCGGAACGCGTCGCCGTTCAAGTTCACCGCGTTCCAGGCCGGGCCGCGGATCTGCCTCGGCAAGGACTCCGCCTACCTCCAGATGAAGATGGCGCTCGCCATCCTCTTCCGCTTCTACACCTTCGACCTCGTCGAGGACCACCCCGTCAAGTACCGGATGATGACCATCCTCTCCATGGCTCACGGCCTCAAGGTCCGCGTCTCCACCTCCGTCTGACCCCCGCCGCCGCTCGCCGGCAGCCGCGCCGCCGCCGCCCGTATCGCTTACCGGAGTAGTAAATAAGCCTATGTAATCTGGTTTGAATTTGAAATTTGAATGTACCATGTTTGATTCTAGGATTTGTTGGTCCTAGACCCTGCTTGAAACGGTGCGAATTTCATCTAAATGGTTGAGAAATTTTATCGAAAGCTGTTCCATTCTACGCTACAAATGGTGGGACTGGATTTAAACATTGGCGACGTGGACAAGGCCGTATCACCATGTTTGCACATTTTTAAACCTGTAATCTGGTTTGAATTTGAATGTACCATGACACCATGTTTGCAAAACTTTACATGAATGTTTGAGAAAAAATATGGAGAACTGTTCAA
CDS Sequence
- >LOC_Os03g07250.1
ATGAAGAGCCCCATGGAGGAAGCTCATGCAATGCCAGTGACATCATTCTTCCCAGTAGCAGGAATCCACAAGCTCATAGCTATCTTCCTTGTTGTCCTCTCATGGATCTTGGTCCACAAGTGGAGCCTGAGGAACCAGAAAGGGCCAAGATCATGGCCAATCATCGGCGCGACAGTGGAGCAACTGAAGAACTACCACAGGATGCATGACTGGCTTGTCGAGTACTTGTCGAAGGACAGGACGGTGACCGTCGACATGCCTTTCACCTCCTACACCTACATTGCCGACCCGGTGAACGTCGAGCATGTCCTGAAGACCAACTTCACCAATTACCCCAAGGGTGAAGTGTACAGGTCTTACATGGATGTGCTGCTCGGTGATGGCATATTCAATGCCGACGGCGAGATGTGGAGGAAGCAAAGGAAGACGGCGAGCTTCGAGTTTGCCTCCAAGAACTTGAGAGACTTCAGCACTGTGGTGTTCAGGGAGTACTCCCTGAAGCTATCAAGCATTCTGAGCCAAGCATGCAAGGCCGGCAGAGTTGTAGACATGCAGGAATTGTTCATGAGGATGACACTGGACTCGATCTGCAAGGTCGGGTTTGGGGTTGAGATCGGGACGCTGTCACCTGATCTCCCGGAGAACAGCTTTGCCCAGGCATTCGACGCTGCCAACATCATCGTCACGCTGCGGTTCATCGATCCTCTGTGGCGTCTGAAGAAGTTCTTGCACGTCGGATCAGAGGCTCTCCTCGAGCAGAGCATGAAGCTGGTTGATGACTTCACCTACAGCGTGATCCGCCGCCGCAAGGCTGAGATCTTGCAGGCTCGAGCCAGCGGCAAGCAAGAGAAGATCAAGCACGACATACTGTCGCGGTTCATCGAGCTCGGGGAGGCCGGCGGCGACGAGGGGGGCGGCAGCTTCGGGGACGACAAGAGCCTCCGCGACGTGGTGCTCAACTTCGTGATCGCCGGGCGTGACACGACGGCGACGACGCTGTCGTGGTTCACGTACATGGCGATGACGCACCCGGCCGTCGCCGACAAGCTCCGGCGCGAGCTGGCCGCGTTCGAGGATGAGCGCGCGCGCGAGGAGGGCGTCGCGCTCGCCGACGCCGCCGGCGAGGCGTCGTTCGCGGCGCGCGTGGCGCAGTTCGCGTCGCTGCTGAGCTACGACGCGGTGGGGAAGCTGGTGTACCTGCACGCGTGCGTGACGGAGACGCTCCGCCTCTACCCGGCGGTGCCGCAGGACCCCAAGGGGATCGTGGAGGACGACGTGCTCCCCGACGGCACCAAGGTGCGCGCCGGCGGGATGGTGACGTACGTGCCCTACTCCATGGGGAGGATGGAGTACAACTGGGGCCCCGACGCGGCGAGCTTCCGGCCGGAGCGGTGGCTCAGCGGCGACGGCGGCGCGTTCCGGAACGCGTCGCCGTTCAAGTTCACCGCGTTCCAGGCCGGGCCGCGGATCTGCCTCGGCAAGGACTCCGCCTACCTCCAGATGAAGATGGCGCTCGCCATCCTCTTCCGCTTCTACACCTTCGACCTCGTCGAGGACCACCCCGTCAAGTACCGGATGATGACCATCCTCTCCATGGCTCACGGCCTCAAGGTCCGCGTCTCCACCTCCGTCTGA
Protein Sequence
- >LOC_Os03g07250.1
MKSPMEEAHAMPVTSFFPVAGIHKLIAIFLVVLSWILVHKWSLRNQKGPRSWPIIGATVEQLKNYHRMHDWLVEYLSKDRTVTVDMPFTSYTYIADPVNVEHVLKTNFTNYPKGEVYRSYMDVLLGDGIFNADGEMWRKQRKTASFEFASKNLRDFSTVVFREYSLKLSSILSQACKAGRVVDMQELFMRMTLDSICKVGFGVEIGTLSPDLPENSFAQAFDAANIIVTLRFIDPLWRLKKFLHVGSEALLEQSMKLVDDFTYSVIRRRKAEILQARASGKQEKIKHDILSRFIELGEAGGDEGGGSFGDDKSLRDVVLNFVIAGRDTTATTLSWFTYMAMTHPAVADKLRRELAAFEDERAREEGVALADAAGEASFAARVAQFASLLSYDAVGKLVYLHACVTETLRLYPAVPQDPKGIVEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGPDAASFRPERWLSGDGGAFRNASPFKFTAFQAGPRICLGKDSAYLQMKMALAILFRFYTFDLVEDHPVKYRMMTILSMAHGLKVRVSTSV*