Information report for DPW
Gene Details
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Functional Descriptions
- We report here the isolation and characterization of a rice (Oryza sativa) male-sterile mutant, defective pollen wall (DPW), which displays defective anther development and degenerated pollen grains with an irregular exine.
- Moreover, we demonstrate that the monocot DPW from rice complements the dicot Arabidopsis thaliana male sterile2 (ms2) mutant and is the probable ortholog of MS2.
- Using map-based cloning, we identified the DPW gene, which is expressed in both tapetal cells and microspores during anther development.
- Chemical analysis revealed that DPW anthers had a dramatic reduction in cutin monomers and an altered composition of cuticular wax, as well as soluble fatty acids and alcohols.
- These data suggest that DPWs participate in a conserved step in primary fatty alcohol synthesis for anther cuticle and pollen sporopollenin biosynthesis in monocots and dicots.
Functional Keywords
- sterile , microspore , anther , tapetal , pollen , anther-development , grain , cuticle , cutin
Literature and News
- Defective pollen wall is required for anther and microspore development in rice and encodes a fatty acyl carrier protein reductase . DOI: 10.1105/tpc.111.087528 ; PMID: 21705642
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice . DOI: 10.1186/s12284-020-00451-y ; PMID: 33409767
Gene Resources
- UniProt: Q0DUU1
- EMBL: AC073556, AP008209
- EnsemblPlants: Os03t0167600-01
- Gramene: Os03t0167600-01
- KEGG: dosa:Os03g0167600
- Orthologous matrix: WRDAQER
- InterPro: IPR013120, IPR026055, IPR033640
- PANTHER: PTHR11011, PTHR11011:SF45
- SUPFAM: SSF51735
- Gene3D: 3.40.50.720
- OrthoDB: Q0DUU1
- SWISS-MODEL: Q0DUU1
- Conserved Domain Database: cd05236, cd09071
Sequences
cDNA Sequence
- >LOC_Os03g07140.1
GATGGCCTCCTCCTGCAATCTGCAAGACTGAAGCTAAGCAGCTTTAAATCCTCCTGCGTCATTTAACCCCCTCTCTGCCTCTCTGAGCTCTGAGCTGCTCAGCTACAGCTCCCTAGCTGTTCTGTTCGATTAATTCAGCTTCGCTTTGCTTGGATTAATTTGTAGGTGACTAACTCGATCACAGCTCAGGTGATTGGCAGTTTGATCACAAGGCAGGAAGGAAGTAGAAGTAGATTGCTGTAGCACACGCTCACCTACCAAGCTTCTACACTCGCAGCCAAGCTTTGATCGTTTGCTTGCTTTGCATTGCTTGGGCTTTGTGGGCAAGAACGTGTCTATCTTCTTCTTGTTCGTCTCGTCAGGCGGCCATGGGGATGAGTTCATGCGTGAACCTATCCCGTGTCGCCGCCGCCGCCGCAGGGAGACGGCCGGGATTTGCCGGTGAGCTGGGAGGTCGCCGTGGTCATGGCAGAAGCGTGCTTCCGGTTGTTGCTGCACTGCCGGTGAGGAGGAAGGGAAGCGGTTGTGGCGTGGCGTGCTGCGTCTCCTCCTCGTCTTCCTCCTCCGTGCACGGCAAGAACTCGGCGGCGGCGGCGGAGGGTCACGCCGGCGGCATTGGGATCGCGGAGTTCCTCGGCGGCAAGAACTTCCTCATCACCGGCGGAACCGGGTTCCTGGCAAAAGTTCTCATCGAGAAGATATTGCGGACGAATCCCGACGTTGGCAAGATCTACGTGCTGATCAAGGCGAAGGACGGTGACGCAGCACTGAAAAGATTGCACAACGAGGTTGTGGACACAGAATTGTTCAGTCGTTTGCAGGAAATCCATGGCAAGGATTACCACAGCTTCGCGGCAAGAAAGCTGGTGCCTGTAGTCGGAGATGTCAGAGAAGCCAACGTTGGCATCGCCCCTGAGCTTGCCGGCGTGATCGCCGACGAGGTCGACATCATCGTCAACTCAGCAGCAAACACCACTTTCGACGAGAGGTACGATGTTGCAATGGACATCAACACCGTAGGGCCATTCAGGATAATGAGCTTCGCGCAGCGGTTTCGGAGGCTGAAGCTGTTCTTGCAAGTGTCAACAGCATATGTGAACGGACAGAGGCAAGGTGTGGTGCTAGAGAAGCCATTTCGCTTGGGGGACACCATAGCCAAGGAGTTAGGATCTCCAGATTCTTCACAACACAAGAACACCATGCTTGACATCGAGGCAGAGATCAAGCTGGCTTTTGACCACAGGAGACACGGTGATGATTCAGCCTCTTTCTCTGAAGAAATGAAAGAGTTAGGATTAGAGAGGGCAAAACTCCATGGGTGGCAAGATACATATGTGTTCACCAAGGCCATGGGAGAGATGGTGATCAACTCCATGCGAGGAGATATACCGGTTGTCACCATCAGGCCAAGCGTCATCGAGAGCACCTGGAGGGATCCCTTCCCCGGTTGGATGGAAGGGAACAGGATGATGGATCCTGTTGTGCTGTACTACGGCAAAGGCCAGCTGAGCGGGTTCCTCGCTGACCCGGAGGGTGTTCTTGACGTGGTTCCGGCGGACATGGTGGTGAACGCAACGCTGGCGTCCATGGCGAAGCACGGGCGCGGCGGCGCGGCGGCGGCGGCGGCGGCGGCGGAGGGGATGCACGTGTACCACGTGGCGTCGTCGACGGTGAACCCGCTGGCGTTCGGCGACCTGAGCCGGTTCCTCTTCCAGCACTTCACGGGGTCGCCGTACAGCGACGCGGCGGGGCGGCCCATCCACGTGCCGCCGATGCGGCTGTTCGACACCATGGAGCAGTTCGCCAGCTACGTCGAGACCGACGCGCTGCTGCGCGCCGGCCGCCTCGCCGGCGCCGGCGCCGGCGCCGGAGACGAGAGGGTGTCGCAGCGGCTGCGCGAGCTCTGCGCCAAGTCCGTCGAGCAGACCATCTACCTCGGCAGCATCTACCAGCCCTACACCTTCTACGGCGGCAGGTTCGACAATGGGAACACGGAGGCGCTGATCGGGGAGATGTCGGAGGAGGAGAAGGCGCGGTTCCACTTCGACGTGAGGAGCATCGAGTGGACGGACTACATCACCAACGTGCACATCCCGGGGCTCAGGAAGCACGTCATGAAGGGGAGGGGCGTCGGCGGCGGCAGCGGCGCGTCGTCGTCATCGAACGCCTCGCTGCTCGCCGGCGCCTCCGTTTGAATGGCCAGGCCGGCGCCGGAGAAGAGGAGGATGCATGCGCGGCGTGTACACTAGGCTAGATTGTTGTTAACTTGTTCTGCCGTGGCAGTCTGACAGATGATCTTTGTGCTGCAGTAATTAAGGTTGATAGTATGTTATCTCTATCACTATGTAATCAGAGCAAAAATTGGCAATCAATTTAACAGAATTTACAAAAGATGCACATAATTCAGAATATAATTTAGCAGAGAGAACATCGGTTGACCAAGTTTTTGGCTTTGATCAAGTACATGTCTAGAACAGTATGGAGTACTAGTAGAGTAGTGCGAAATTTGAAAACCAGAATGTCAGATACATGCGAAGAAAAACATCCAACTAGAGAAAAAGGTGCCATGGAACACTGCACTGTAGCACATCAGGGTAACCACCCCCAAAATTCGGAATACTACACCGAAAGATGATTAGGTTGCCTTGCCATTGATGATACAAATTACAAGACTATCGATACAAGCTTACGCTACAAAATACAAATAGAAGTTCAGTCTTGCCACTAGATTTCCTCTGTAAAAATGCCTAAAAAGGAGAGGGTGAGGATGGATGGGCCTTTTTTATCACCTGAAAAACTCAGGCATCGCTGCATGAGCATGACACTTTGTTTCAGGATAGCAGCATCTCTTGCATTGACCAATGGAGGGTCAAGTTTTACGAAGGGATCTGATGTTCCCTTGTTATTGTTGGTTGGCCTATGCGCAGGTTTCACAGTCCAGCAAAGTATCACTGACCAGCATTTGACTATCGCAAATTCGCAGTACAAGGTGACAAATGGTAGCACCATTAACTCAAAGGAAATAATCGGGCGTCCTCCGTGTCACGTCAGGCTCACCTCTCCTAGGAGCTGGTTCGAACTGCAACAA
CDS Sequence
- >LOC_Os03g07140.1
ATGGGGATGAGTTCATGCGTGAACCTATCCCGTGTCGCCGCCGCCGCCGCAGGGAGACGGCCGGGATTTGCCGGTGAGCTGGGAGGTCGCCGTGGTCATGGCAGAAGCGTGCTTCCGGTTGTTGCTGCACTGCCGGTGAGGAGGAAGGGAAGCGGTTGTGGCGTGGCGTGCTGCGTCTCCTCCTCGTCTTCCTCCTCCGTGCACGGCAAGAACTCGGCGGCGGCGGCGGAGGGTCACGCCGGCGGCATTGGGATCGCGGAGTTCCTCGGCGGCAAGAACTTCCTCATCACCGGCGGAACCGGGTTCCTGGCAAAAGTTCTCATCGAGAAGATATTGCGGACGAATCCCGACGTTGGCAAGATCTACGTGCTGATCAAGGCGAAGGACGGTGACGCAGCACTGAAAAGATTGCACAACGAGGTTGTGGACACAGAATTGTTCAGTCGTTTGCAGGAAATCCATGGCAAGGATTACCACAGCTTCGCGGCAAGAAAGCTGGTGCCTGTAGTCGGAGATGTCAGAGAAGCCAACGTTGGCATCGCCCCTGAGCTTGCCGGCGTGATCGCCGACGAGGTCGACATCATCGTCAACTCAGCAGCAAACACCACTTTCGACGAGAGGTACGATGTTGCAATGGACATCAACACCGTAGGGCCATTCAGGATAATGAGCTTCGCGCAGCGGTTTCGGAGGCTGAAGCTGTTCTTGCAAGTGTCAACAGCATATGTGAACGGACAGAGGCAAGGTGTGGTGCTAGAGAAGCCATTTCGCTTGGGGGACACCATAGCCAAGGAGTTAGGATCTCCAGATTCTTCACAACACAAGAACACCATGCTTGACATCGAGGCAGAGATCAAGCTGGCTTTTGACCACAGGAGACACGGTGATGATTCAGCCTCTTTCTCTGAAGAAATGAAAGAGTTAGGATTAGAGAGGGCAAAACTCCATGGGTGGCAAGATACATATGTGTTCACCAAGGCCATGGGAGAGATGGTGATCAACTCCATGCGAGGAGATATACCGGTTGTCACCATCAGGCCAAGCGTCATCGAGAGCACCTGGAGGGATCCCTTCCCCGGTTGGATGGAAGGGAACAGGATGATGGATCCTGTTGTGCTGTACTACGGCAAAGGCCAGCTGAGCGGGTTCCTCGCTGACCCGGAGGGTGTTCTTGACGTGGTTCCGGCGGACATGGTGGTGAACGCAACGCTGGCGTCCATGGCGAAGCACGGGCGCGGCGGCGCGGCGGCGGCGGCGGCGGCGGCGGAGGGGATGCACGTGTACCACGTGGCGTCGTCGACGGTGAACCCGCTGGCGTTCGGCGACCTGAGCCGGTTCCTCTTCCAGCACTTCACGGGGTCGCCGTACAGCGACGCGGCGGGGCGGCCCATCCACGTGCCGCCGATGCGGCTGTTCGACACCATGGAGCAGTTCGCCAGCTACGTCGAGACCGACGCGCTGCTGCGCGCCGGCCGCCTCGCCGGCGCCGGCGCCGGCGCCGGAGACGAGAGGGTGTCGCAGCGGCTGCGCGAGCTCTGCGCCAAGTCCGTCGAGCAGACCATCTACCTCGGCAGCATCTACCAGCCCTACACCTTCTACGGCGGCAGGTTCGACAATGGGAACACGGAGGCGCTGATCGGGGAGATGTCGGAGGAGGAGAAGGCGCGGTTCCACTTCGACGTGAGGAGCATCGAGTGGACGGACTACATCACCAACGTGCACATCCCGGGGCTCAGGAAGCACGTCATGAAGGGGAGGGGCGTCGGCGGCGGCAGCGGCGCGTCGTCGTCATCGAACGCCTCGCTGCTCGCCGGCGCCTCCGTTTGA
Protein Sequence
- >LOC_Os03g07140.1
MGMSSCVNLSRVAAAAAGRRPGFAGELGGRRGHGRSVLPVVAALPVRRKGSGCGVACCVSSSSSSSVHGKNSAAAAEGHAGGIGIAEFLGGKNFLITGGTGFLAKVLIEKILRTNPDVGKIYVLIKAKDGDAALKRLHNEVVDTELFSRLQEIHGKDYHSFAARKLVPVVGDVREANVGIAPELAGVIADEVDIIVNSAANTTFDERYDVAMDINTVGPFRIMSFAQRFRRLKLFLQVSTAYVNGQRQGVVLEKPFRLGDTIAKELGSPDSSQHKNTMLDIEAEIKLAFDHRRHGDDSASFSEEMKELGLERAKLHGWQDTYVFTKAMGEMVINSMRGDIPVVTIRPSVIESTWRDPFPGWMEGNRMMDPVVLYYGKGQLSGFLADPEGVLDVVPADMVVNATLASMAKHGRGGAAAAAAAAEGMHVYHVASSTVNPLAFGDLSRFLFQHFTGSPYSDAAGRPIHVPPMRLFDTMEQFASYVETDALLRAGRLAGAGAGAGDERVSQRLRELCAKSVEQTIYLGSIYQPYTFYGGRFDNGNTEALIGEMSEEEKARFHFDVRSIEWTDYITNVHIPGLRKHVMKGRGVGGGSGASSSSNASLLAGASV*