Gene Details:

Functional Descriptions:

  • Systematic analyses of the transgenic rice indicated that co-overexpression of OsBMY4 and OsISA3 not only promoted rice yield and quality, but also improved seed germination and stress tolerance.
  • In this study, OsBMY4 and OsISA3, the key β-amylase and debranching enzymes that control transient starch degradation in rice leaves, were co-overexpressed in rice in order to accelerate starch degradation efficiency and increase the sugar supply for sink organs.
  • Moreover, since the OsBMY4 gene has not been characterized, we generated OsBMY4 mutants using CRIPSR/Cas9 gene editing, which helped to reveal the roles of β-amylase in rice yield and quality.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g04770.1
    CGGTAACCCTCACAATCCCGTTCGTCGAGATCGTCGCTTCGGCGTAACAACAAATAAGGGGAGATCCCCAAGTCCTAACCCGAATCCATTTCCAATAATCCATTTCGCCACGATTTGTTCTTGGTTGTCTGAGAGAGAGAGAGAGAGAGATCCGTATACATTGCTGCTACTTGAGGTCAAGCCCGTTCGTTCGCGCGCGCGCGCCTGTCTGTCTCTGTTTGATTGATTCTTCTACGTCGTCGACGTACGCCTGTTCATGATGTCGCTCAACCTGGCTCACCAGACCGGAGCGGCGGCCGCCGTGGCACCGGCGGCGCCACGTACGGCCGTGGTCGCGGCGGCTGCGGGTACAGTGTCTGCTCCGGCGGTCGCGCCGGCGGCGGCGCCGAGCCTGCAGCTGCAGACGCAGACTGTGGACCCGGCGGCGCCGGCGCAGGGGCCGGACCTCCCCATGGCCTTCCAGGCGCTGGTCGAGAGCCTGCCGGAGGAGCAGCACCCGGACGTGGGCGGCGAGGAGCGGCGCAAGGTGGGCGTGCCGGTGTACGTGATGATGCCGCTGGACACGGTGCGCAAGGACGGCAACGGGCTGAACCGGCGGAAGGCGGTGGAGGCGTCCCTGAAGGCGCTGAAGAGCGCCGGCGCCGAGGGGATCATGGTGGACGTGTGGTGGGGCATCGCCGAGTGCGAGGGCCCCGGCCGCTACAACTTCACCGGGTACATGGAGCTCATGGAGATGGCCAAGAAGAACGGGCTCAAGGTGCAGGCCGTCATGTCGTTCCACCAGTGTGGCGGCAACGTCGGCGACTCAGTCACTATACCACTTCCGAAATGGGTGTTGGAGGAGATGGACAAGGACCAGGACCTGGCCTACACGGATCGGAGCGGCCGCCGCAACTACGAATACCTCTCGCTCGGCGCCGACGCCATGCCGGTGCTCAAGGGCCGCACGCCGGTGCAGTGCTACGGCGACTTCATGCGCGCCTTCCGCGACCACTTCGCCGCCTTCATGGGCAACACCATAGTCGAGATTCAAGTCGGCATGGGTCCGGCCGGTGAGCTCCGCTACCCGTCGTACCCGGAGAGCAATGGCACCTGGAGATTCCCCGGCATCGGCGAGTTCCAGTGCTATGACAGGTACATGCTGAGCAGTCTCAAGGCGGCGGCGGAGGCGGTGGGCAAGCCGGAGTGGGGCAACGCCGGGCCGGGAGACTCCGGCGGGTACAACGACTGGCCGGAGGACTCGCCCTTCTTCCGCCGTGAAGGTGGGTGGAACACTCCCTACGGTGAGTTTTTCATGAGCTGGTACTCGCAGATGCTCCTGGAACACGGCGAGCGCATCCTGTCGGCGGCGTCGGGCGTGTACACCGGCACACCCGGCGTGAAGATCTCCGTGAAGGTGGCCGGCATCCACTGGCACTACGGCACGCGGTCCCACGCCGCGGAGCTGACGGCAGGGTACTACAACACGAGGCACCACGACGGGTACCAGCCGATCGCGCGCATGCTGGCCCGCCACGGCGCGGTGCTCAACTTCACGTGCGTGGAGATGCGCAACCACGAGCAGCCGCAGGACGCGCAGTGCCGGCCCGAGGAGCTGGTGCAGCAGGTGGCGGCCGCGGCGCGGGAGTCCGGCGTGGGGCTCGCCGGCGAGAACGCGCTGCCGAGGTACGACGAGACGGCGCACGACCAGATCGTGACGACGGCGGCGGAGAAGGCGGAGGAGGAGCGCATGGTAGCGTTCACCTACCTGCGCATGGGGCCCGACCTGTTCCAGCCGGACAACTGGCGCCGCTTCGCCGCGTTCGTGAAGCGCATGACGGAGTCCGGCGTGCGGGACGTGTGCCGCGAGCAGGTGGAGCGGGAGGCGCAGGGCGTCGCGCACGCCACCGGGTCGCTCGTGCACGAGGCCGCCGTCGCGCTCAGCAACTGATCGATCAGCCGACCTCGCGCGTAGAAGGGTAAAAACGCTTTACATATTTTATACTCCCAAGATTATTTGTTGTAATCAGCGTCGCGTACGCGTGCGACGACATCGATCGCTCAAGTGTGCAACCTTGATCTGCGAAAAGAAGAAGAAAAACATAGTGTGGTGTGCGTGTGTGTGTGTGTGTGTGGGGGGGGGGGGGGGGGGTTACTGGGTAAGTACGTGTACGTAGTTGCCACGTTTCAGGCTTTTGATGCTGGCCGATTCTGGCATCGCTCAAAGCTTTTGCTTTTGCAGGAAATGGATGTAACTATTACATTTTCTATCATAAGAAAATACATGTACTTCAAAACCCGAAATGCCAGTACTACATTCGTGCA
CDS Sequence
  • >LOC_Os03g04770.1
    ATGATGTCGCTCAACCTGGCTCACCAGACCGGAGCGGCGGCCGCCGTGGCACCGGCGGCGCCACGTACGGCCGTGGTCGCGGCGGCTGCGGGTACAGTGTCTGCTCCGGCGGTCGCGCCGGCGGCGGCGCCGAGCCTGCAGCTGCAGACGCAGACTGTGGACCCGGCGGCGCCGGCGCAGGGGCCGGACCTCCCCATGGCCTTCCAGGCGCTGGTCGAGAGCCTGCCGGAGGAGCAGCACCCGGACGTGGGCGGCGAGGAGCGGCGCAAGGTGGGCGTGCCGGTGTACGTGATGATGCCGCTGGACACGGTGCGCAAGGACGGCAACGGGCTGAACCGGCGGAAGGCGGTGGAGGCGTCCCTGAAGGCGCTGAAGAGCGCCGGCGCCGAGGGGATCATGGTGGACGTGTGGTGGGGCATCGCCGAGTGCGAGGGCCCCGGCCGCTACAACTTCACCGGGTACATGGAGCTCATGGAGATGGCCAAGAAGAACGGGCTCAAGGTGCAGGCCGTCATGTCGTTCCACCAGTGTGGCGGCAACGTCGGCGACTCAGTCACTATACCACTTCCGAAATGGGTGTTGGAGGAGATGGACAAGGACCAGGACCTGGCCTACACGGATCGGAGCGGCCGCCGCAACTACGAATACCTCTCGCTCGGCGCCGACGCCATGCCGGTGCTCAAGGGCCGCACGCCGGTGCAGTGCTACGGCGACTTCATGCGCGCCTTCCGCGACCACTTCGCCGCCTTCATGGGCAACACCATAGTCGAGATTCAAGTCGGCATGGGTCCGGCCGGTGAGCTCCGCTACCCGTCGTACCCGGAGAGCAATGGCACCTGGAGATTCCCCGGCATCGGCGAGTTCCAGTGCTATGACAGGTACATGCTGAGCAGTCTCAAGGCGGCGGCGGAGGCGGTGGGCAAGCCGGAGTGGGGCAACGCCGGGCCGGGAGACTCCGGCGGGTACAACGACTGGCCGGAGGACTCGCCCTTCTTCCGCCGTGAAGGTGGGTGGAACACTCCCTACGGTGAGTTTTTCATGAGCTGGTACTCGCAGATGCTCCTGGAACACGGCGAGCGCATCCTGTCGGCGGCGTCGGGCGTGTACACCGGCACACCCGGCGTGAAGATCTCCGTGAAGGTGGCCGGCATCCACTGGCACTACGGCACGCGGTCCCACGCCGCGGAGCTGACGGCAGGGTACTACAACACGAGGCACCACGACGGGTACCAGCCGATCGCGCGCATGCTGGCCCGCCACGGCGCGGTGCTCAACTTCACGTGCGTGGAGATGCGCAACCACGAGCAGCCGCAGGACGCGCAGTGCCGGCCCGAGGAGCTGGTGCAGCAGGTGGCGGCCGCGGCGCGGGAGTCCGGCGTGGGGCTCGCCGGCGAGAACGCGCTGCCGAGGTACGACGAGACGGCGCACGACCAGATCGTGACGACGGCGGCGGAGAAGGCGGAGGAGGAGCGCATGGTAGCGTTCACCTACCTGCGCATGGGGCCCGACCTGTTCCAGCCGGACAACTGGCGCCGCTTCGCCGCGTTCGTGAAGCGCATGACGGAGTCCGGCGTGCGGGACGTGTGCCGCGAGCAGGTGGAGCGGGAGGCGCAGGGCGTCGCGCACGCCACCGGGTCGCTCGTGCACGAGGCCGCCGTCGCGCTCAGCAACTGA
Protein Sequence
  • >LOC_Os03g04770.1
    MMSLNLAHQTGAAAAVAPAAPRTAVVAAAAGTVSAPAVAPAAAPSLQLQTQTVDPAAPAQGPDLPMAFQALVESLPEEQHPDVGGEERRKVGVPVYVMMPLDTVRKDGNGLNRRKAVEASLKALKSAGAEGIMVDVWWGIAECEGPGRYNFTGYMELMEMAKKNGLKVQAVMSFHQCGGNVGDSVTIPLPKWVLEEMDKDQDLAYTDRSGRRNYEYLSLGADAMPVLKGRTPVQCYGDFMRAFRDHFAAFMGNTIVEIQVGMGPAGELRYPSYPESNGTWRFPGIGEFQCYDRYMLSSLKAAAEAVGKPEWGNAGPGDSGGYNDWPEDSPFFRREGGWNTPYGEFFMSWYSQMLLEHGERILSAASGVYTGTPGVKISVKVAGIHWHYGTRSHAAELTAGYYNTRHHDGYQPIARMLARHGAVLNFTCVEMRNHEQPQDAQCRPEELVQQVAAAARESGVGLAGENALPRYDETAHDQIVTTAAEKAEEERMVAFTYLRMGPDLFQPDNWRRFAAFVKRMTESGVRDVCREQVEREAQGVAHATGSLVHEAAVALSN*