Information report for CEBiP;OsCEBiP
Gene Details
|
|
Functional Descriptions
- Here, we report that the perception of CE by CEBiP contributes to disease resistance against the rice blast fungus, Magnaporthe oryzae, and that enhanced responses to CE by engineering CEBiP increase disease tolerance.
- However, the single/triple knockout (KO) mutants of Arabidopsis CEBiP homologs and the overexpressor of AtCEBiP showed chitin-induced defense responses similar to wild-type Arabidopsis, indicating that AtCEBiP is biochemically functional as a chitin-binding protein but does not contribute to signaling.
- To enhance defense responses to CE, we constructed chimeric genes composed of CEBiP and Xa21, which mediate resistance to rice bacterial leaf blight.
- The expression of either CRXa1 or CRXa3, each of which contains the whole extracellular portion of CEBiP, the whole intracellular domain of XA21, and the transmembrane domain from either CEBiP or XA21, induced cell death accompanied by an increased production of reactive oxygen and nitrogen species after treatment with CE.
- Knockdown of CEBiP gene by RNA interference resulted in the suppression of the elicitor-induced oxidative burst as well as the gene responses, showing that CEBiP plays a key role in the perception and transduction of chitin oligosaccharide elicitor in the rice cells.
- In rice (Oryza sativa), the chitin elicitor binding protein (CEBiP) recognizes chitin oligosaccharides released from the cell walls of fungal pathogens.
- Chitin is the major component of fungal cell wall and serves as a molecular pattern that can be recognized by the receptor OsCEBiP in rice, a lysine motif (LysM) receptor-like protein (RLP), to trigger immune responses.
Functional Keywords
- magnaporthe-oryzae , defense-response , blight , nitrogen , oxidative , disease-resistance , leaf , disease , blast , defense , cell-wall , cell-death , immune-response
Literature and News
- An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 module is an essential early component of chitin-induced rice immunity . DOI: 10.1016/j.chom.2013.03.007 ; PMID: 23601108
- Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor . DOI: 10.1073/pnas.0508882103 ; PMID: 16829581
- Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice . DOI: 10.1111/j.1365-313X.2010.04328.x ; PMID: 21070413
- OsLYP4 and OsLYP6 play critical roles in rice defense signal transduction . DOI: 10.4161/psb.22980 ; PMID: 23299421
- Effector-mediated suppression of chitin-triggered immunity by magnaporthe oryzae is necessary for rice blast disease . DOI: 10.1105/tpc.111.092957 ; PMID: 22267486
- CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis . DOI: 10.1073/pnas.0705147104 ; PMID: 18042724
- Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice . DOI: 10.1111/j.1365-313X.2010.04324.x ; PMID: 21070404
- Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants . DOI: 10.1093/pcp/pcs113 ; PMID: 22891159
- The bifunctional plant receptor, OsCERK1, regulates both chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice . DOI: 10.1093/pcp/pcu129 ; PMID: 25231970
- Molecular Mechanism for Fungal Cell Wall Recognition by Rice Chitin Receptor OsCEBiP . DOI: 10.1016/j.str.2016.04.014 ; PMID: 27238968
- Integrated Metabarcoding and Culturomic-Based Microbiome Profiling of Rice Phyllosphere Reveal Diverse and Functional Bacterial Communities for Blast Disease Suppression . DOI: 10.3389/fmicb.2021.780458 ; PMID: 34917058
Gene Resources
- UniProt: Q8H8C7
- EMBL: AB206975, AC099399, AK073032
- AlphaFoldDB: Q8H8C7
- EnsemblPlants: Os03t0133400-01
- Gramene: Os03t0133400-01
- KEGG: dosa:Os03g0133400
- Orthologous matrix: CAYTGYS
- InterPro: IPR018392, IPR036779
- PANTHER: PTHR33734, PTHR33734:SF31
- SUPFAM: SSF54106
- PROSITE: PS51782
- Gene3D: 3.10.350.10
- SWISS-MODEL: Q8H8C7
- Conserved Domain Database: cd00118
- eggNOG: ENOG502QQ9K
Sequences
cDNA Sequence
- >LOC_Os03g04110.1
GAGGCATACTCCTCCTGCTCCAGTGTCAACACGACAGTCAAACAAACACCAAGCCTCTCATTCCCACCTCTCGAGGAGAGCTTTCCCCACCATGGCGTCGCTCACCGCCGCCCTGGCCACGCCGGCGGCCGCTGCCCTCCTCCTCCTCGTCCTCCTCGCCGCCCCCGCCTCCGCCGCCAACTTCACCTGCGCGGTGGCTTCAGGCACCACCTGCAAGTCCGCCATCCTCTACACCTCCCCCAACGCCACCACCTACGGCAACCTCGTCGCCCGCTTCAACACCACCACCCTCCCCGACCTCCTCGGCGCCAACGGCCTCCCCGACGGCACGCTTTCCTCCGCCCCCGTCGCCGCCAATTCCACCGTCAAAATCCCCTTCCGCTGCCGCTGCAACGGCGACGTCGGCCAGTCGGACCGCCTCCCCATCTACGTCGTGCAGCCGCAGGACGGGCTCGACGCCATCGCGCGCAACGTGTTCAACGCCTTCGTCACCTACCAGGAGATCGCCGCCGCGAACAACATCCCCGACCCCAACAAGATAAATGTCAGCCAGACGCTGTGGATTCCGCTGCCCTGCAGCTGCGACAAGGAGGAAGGCTCTAACGTGATGCACCTCGCCTACAGCGTCGGCAAAGGGGAGAACACGTCGGCGATCGCTGCCAAGTACGGGGTGACGGAGTCCACGCTTCTCACCAGAAATAAGATCGACGACCCCACGAAATTGCAGATGGGACAGATTCTAGATGTCCCGCTCCCTGTGTGCCGTTCATCAATCAGCGATACCTCAGCTGATCACAATCTGATGCTCCTCCCGGATGGCACCTATGGATTCACCGCAGGAAACTGCATCCGCTGCAGCTGCAGTTCAACTACCTACCAGCTAAACTGCACTGCAGTACAGAACAAGGGATGCCCGTCAGTGCCACTGTGCAATGGAACGCTGAAGCTTGGTGAGACGAACGGCACCGGTTGCGGATCAACAACGTGCGCCTACAGTGGTTACTCCAACAGTTCATCGCTCATCATACAAACCAGCCTTGCAACTAATCAGACAACAGCCTGCCAGAGAGGAGGATCTGGGAGGTCGCAGTTCGCTAGGTCCATGTGGAGCATGTCTGTTATCTCCTTCCACATGGTGTTGATCATTATCTGTTTCCTTTGATGTTGGAGACTACTGCAACTCTAGATGGTACATTTCAAAGAGTTCTCTACGATCTATGATTGTTGTATACGATATATGATTGTTGTCGTAACTTAGATTTTGATGACTGGTTTATCCAGCTTTGAAATTTGAGTTTTGACTCTGTTCTTTAGAGGATGAGTGGCACTTGTACGGCTGCTTGAATAAAACGTCGATGTATTGTATTCGATCTGCATCTGAAAAGGAATATTCATTAGATAGGATTATTCGAAATAAAAAGATCCCACATGTTTTTGTTATAGGCGTTACATGTTCTGGATGGATATATAATGTACCATGCGGAAGTATGAATGGACTTCCCACCTTCCAATCTTCCATCTACACTTCAAGCCTTGCATAAAGCAAAATTGATCTTAGCAA - >LOC_Os03g04110.2
GAGGCATACTCCTCCTGCTCCAGTGTCAACACGACAGTCAAACAAACACCAAGCCTCTCATTCCCACCTCTCGAGGAGAGCTTTCCCCACCATGGCGTCGCTCACCGCCGCCCTGGCCACGCCGGCGGCCGCTGCCCTCCTCCTCCTCGTCCTCCTCGCCGCCCCCGCCTCCGCCGCCAACTTCACCTGCGCGGTGGCTTCAGGCACCACCTGCAAGTCCGCCATCCTCTACACCTCCCCCAACGCCACCACCTACGGCAACCTCGTCGCCCGCTTCAACACCACCACCCTCCCCGACCTCCTCGGCGCCAACGGCCTCCCCGACGGCACGCTTTCCTCCGCCCCCGTCGCCGCCAATTCCACCGTCAAAATCCCCTTCCGCTGCCGCTGCAACGGCGACGTCGGCCAGTCGGACCGCCTCCCCATCTACGTCGTGCAGCCGCAGGACGGGCTCGACGCCATCGCGCGCAACGTGTTCAACGCCTTCGTCACCTACCAGGAGATCGCCGCCGCGAACAACATCCCCGACCCCAACAAGATAAATGTCAGCCAGACGCTGTGGATTCCGCTGCCCTGCAGCTGCGACAAGGAGGAAGGCTCTAACGTGATGCACCTCGCCTACAGCGTCGGCAAAGGGGAGAACACGTCGGCGATCGCTGCCAAGTACGGGGTGACGGAGTCCACGCTTCTCACCAGAAATAAGATCGACGACCCCACGAAATTGCAGATGGGACAGATTCTAGATGTCCCGCTCCCTGTGTGCCGTTCATCAATCAGCGATACCTCAGCTGATCACAATCTGATGCTCCTCCCGGATGGCACCTATGGATTCACCGCAGGAAACTGCATCCGCTGCAGCTGCAGTTCAACTACCTACCAGCTAAACTGCACTGCAGTACAGAACAAGGGATGCCCGTCAGTGCCACTGTGCAATGGAACGCTGAAGCTTGGTGAGACGAACGGCACCGGTTGCGGATCAACAACGTGCGCCTACAGTGGTTACTCCAACAGTTCATCGCTCATCATACAAACCAGCCTTGCAACTAATCAGACAACAGCCTGCCAGAGAGGAGGATCTGGGAGGTCGCAGTTCGCTAGGTCCATGTGGAGCATGTCTGTTATCTCCTTCCACATGGTGTTGATCATTATCTGTTTCCTTTGATGTTGGAGACTACTGCAACTCTAGATGGTACATTTCAAAGAGTTCTCTACGATCTATGATTGTTGTATACGATATATGATTGTTGTCGTAACTTAGATTTTGATGACTGGTTTATCCAGCTTTGAAATTTGAGTTTTGACTCTGTTCTTTAGAGGATGAGTGGCACTTGTACGGCTGCTTGAATAAAACGTCGATGTATTGTATTCGATCTGCATCTGAAAAGGAATATTCATTAGATAGGATTATTCGAAATAAAAAGATCCCACATGTTTTTGTTATAGGCGTTACATGTTCTGGATGGATATATAATGTACCATGCGGAAGTATGAATGGACTTCCCACCTTCCAATCTTCCATCTACACTTCAAGCCTTGCATAAAGCAAAATTGATCTTAGCAA
CDS Sequence
- >LOC_Os03g04110.1
ATGGCGTCGCTCACCGCCGCCCTGGCCACGCCGGCGGCCGCTGCCCTCCTCCTCCTCGTCCTCCTCGCCGCCCCCGCCTCCGCCGCCAACTTCACCTGCGCGGTGGCTTCAGGCACCACCTGCAAGTCCGCCATCCTCTACACCTCCCCCAACGCCACCACCTACGGCAACCTCGTCGCCCGCTTCAACACCACCACCCTCCCCGACCTCCTCGGCGCCAACGGCCTCCCCGACGGCACGCTTTCCTCCGCCCCCGTCGCCGCCAATTCCACCGTCAAAATCCCCTTCCGCTGCCGCTGCAACGGCGACGTCGGCCAGTCGGACCGCCTCCCCATCTACGTCGTGCAGCCGCAGGACGGGCTCGACGCCATCGCGCGCAACGTGTTCAACGCCTTCGTCACCTACCAGGAGATCGCCGCCGCGAACAACATCCCCGACCCCAACAAGATAAATGTCAGCCAGACGCTGTGGATTCCGCTGCCCTGCAGCTGCGACAAGGAGGAAGGCTCTAACGTGATGCACCTCGCCTACAGCGTCGGCAAAGGGGAGAACACGTCGGCGATCGCTGCCAAGTACGGGGTGACGGAGTCCACGCTTCTCACCAGAAATAAGATCGACGACCCCACGAAATTGCAGATGGGACAGATTCTAGATGTCCCGCTCCCTGTGTGCCGTTCATCAATCAGCGATACCTCAGCTGATCACAATCTGATGCTCCTCCCGGATGGCACCTATGGATTCACCGCAGGAAACTGCATCCGCTGCAGCTGCAGTTCAACTACCTACCAGCTAAACTGCACTGCAGTACAGAACAAGGGATGCCCGTCAGTGCCACTGTGCAATGGAACGCTGAAGCTTGGTGAGACGAACGGCACCGGTTGCGGATCAACAACGTGCGCCTACAGTGGTTACTCCAACAGTTCATCGCTCATCATACAAACCAGCCTTGCAACTAATCAGACAACAGCCTGCCAGAGAGGAGGATCTGGGAGGTCGCAGTTCGCTAGGTCCATGTGGAGCATGTCTGTTATCTCCTTCCACATGGTGTTGATCATTATCTGTTTCCTTTGA - >LOC_Os03g04110.2
ATGGCGTCGCTCACCGCCGCCCTGGCCACGCCGGCGGCCGCTGCCCTCCTCCTCCTCGTCCTCCTCGCCGCCCCCGCCTCCGCCGCCAACTTCACCTGCGCGGTGGCTTCAGGCACCACCTGCAAGTCCGCCATCCTCTACACCTCCCCCAACGCCACCACCTACGGCAACCTCGTCGCCCGCTTCAACACCACCACCCTCCCCGACCTCCTCGGCGCCAACGGCCTCCCCGACGGCACGCTTTCCTCCGCCCCCGTCGCCGCCAATTCCACCGTCAAAATCCCCTTCCGCTGCCGCTGCAACGGCGACGTCGGCCAGTCGGACCGCCTCCCCATCTACGTCGTGCAGCCGCAGGACGGGCTCGACGCCATCGCGCGCAACGTGTTCAACGCCTTCGTCACCTACCAGGAGATCGCCGCCGCGAACAACATCCCCGACCCCAACAAGATAAATGTCAGCCAGACGCTGTGGATTCCGCTGCCCTGCAGCTGCGACAAGGAGGAAGGCTCTAACGTGATGCACCTCGCCTACAGCGTCGGCAAAGGGGAGAACACGTCGGCGATCGCTGCCAAGTACGGGGTGACGGAGTCCACGCTTCTCACCAGAAATAAGATCGACGACCCCACGAAATTGCAGATGGGACAGATTCTAGATGTCCCGCTCCCTGTGTGCCGTTCATCAATCAGCGATACCTCAGCTGATCACAATCTGATGCTCCTCCCGGATGGCACCTATGGATTCACCGCAGGAAACTGCATCCGCTGCAGCTGCAGTTCAACTACCTACCAGCTAAACTGCACTGCAGTACAGAACAAGGGATGCCCGTCAGTGCCACTGTGCAATGGAACGCTGAAGCTTGGTGAGACGAACGGCACCGGTTGCGGATCAACAACGTGCGCCTACAGTGGTTACTCCAACAGTTCATCGCTCATCATACAAACCAGCCTTGCAACTAATCAGACAACAGCCTGCCAGAGAGGAGGATCTGGGAGGTCGCAGTTCGCTAGGTCCATGTGGAGCATGTCTGTTATCTCCTTCCACATGGTGTTGATCATTATCTGTTTCCTTTGA
Protein Sequence
- >LOC_Os03g04110.1
MASLTAALATPAAAALLLLVLLAAPASAANFTCAVASGTTCKSAILYTSPNATTYGNLVARFNTTTLPDLLGANGLPDGTLSSAPVAANSTVKIPFRCRCNGDVGQSDRLPIYVVQPQDGLDAIARNVFNAFVTYQEIAAANNIPDPNKINVSQTLWIPLPCSCDKEEGSNVMHLAYSVGKGENTSAIAAKYGVTESTLLTRNKIDDPTKLQMGQILDVPLPVCRSSISDTSADHNLMLLPDGTYGFTAGNCIRCSCSSTTYQLNCTAVQNKGCPSVPLCNGTLKLGETNGTGCGSTTCAYSGYSNSSSLIIQTSLATNQTTACQRGGSGRSQFARSMWSMSVISFHMVLIIICFL* - >LOC_Os03g04110.2
MASLTAALATPAAAALLLLVLLAAPASAANFTCAVASGTTCKSAILYTSPNATTYGNLVARFNTTTLPDLLGANGLPDGTLSSAPVAANSTVKIPFRCRCNGDVGQSDRLPIYVVQPQDGLDAIARNVFNAFVTYQEIAAANNIPDPNKINVSQTLWIPLPCSCDKEEGSNVMHLAYSVGKGENTSAIAAKYGVTESTLLTRNKIDDPTKLQMGQILDVPLPVCRSSISDTSADHNLMLLPDGTYGFTAGNCIRCSCSSTTYQLNCTAVQNKGCPSVPLCNGTLKLGETNGTGCGSTTCAYSGYSNSSSLIIQTSLATNQTTACQRGGSGRSQFARSMWSMSVISFHMVLIIICFL*