Gene Details:

Functional Descriptions:

  • Disease resistance phenotype assays revealed that the OsBIRH1-overexpressing transgenic plants showed an enhanced disease resistance against Alternaria brassicicola and Pseudomonas syringae pv.
  • Expression of OsBIRH1 was activated in rice seedling leaves after treatment with defence-related signal chemicals, for example benzothiadiazole, salicylic acid, l-aminocyclopropane-1-carboxylic acid, and jasmonic acid, and was also up-regulated in an incompatible interaction between a resistant rice genotype and the blast fungus, Magnaporthe grisea.
  • The results suggest that OsBIRH1 encodes a functional DEAD-box RNA helicase and plays important roles in defence responses against biotic and abiotic stresses.
  • Moreover, the OsBIRH1 transgenic Arabidopsis plants also showed increased tolerance to oxidative stress and elevated expression levels of oxidative defence genes, AtApx1, AtApx2, and AtFSD1.
  • OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress.
  • The TCD33 seedlings also exhibited less chlorophyll contents and severe defects of chloroplast structure under 20<U+2009>C condition.
  • A DEAD-box RNA helicase TCD33 that confers chloroplast development in rice at seedling stage under cold stress.
  • The transcript expression level of TCD33 indicated that the genes related to chlorophyll (Chl) biosynthesis, photosynthesis, and chloroplast development in TCD33 mutants were down-regulated at 20<U+2009>C, while the down-regulated genes were nearly recovered to or slightly higher than the WT level at 32<U+2009>C.
  • Together, our results suggest that the cold-inducible TCD33 is essential for early chloroplast development and is important for cold-responsive gene regulation and cold tolerance in rice.
  • Map-based cloning and complementation experiments suggested that TCD33 encodes a chloroplast-located DEAD-box RNA helicase protein.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os03g01830.1
    ATCGGATAGGAAGGAAGGAGACGGCGGCGCTGCGAGAGATGGAGGTGGCGGGTGCTCAGGCCGGCATCCTCCCCCTCCTCCTCCGTCACCCCGCGTCCCTCCGGGGCTCCCTCTCCCTCTCCTGCGGCGGCGCCCGCCGGAGCTGGGCGGCGGCGGCGGCCACGGCGGAAGGAGGAGGAGGAGAGGAGGGTCGCGGCTACGAGAGGGTGCCCATGGACACCCCCGGCGCCTACCGCCTCGTCGACCGGGCCACCGGCCGCAGCGTCATCGTGTGGGGCGGCACCGACGACGTCAGCATGCCCTCCCCCGCGGTGCTCTCCACGACCACGCGCGTCCCCGACCGCCCAAAAGAAAATGGGAGAAGTACAAGTATTGGAAATTTTGGCAGACTCAAGGCACAGAAAGTTAAAGTTTTAGCAAGGAGATCAGCTCACCTTAAGAGAGAGGATTCTGGTCGCATAAGCACCAGCCGGTTCAGTGAATCTCCTTCTGATGAGTCTGACGAAGATGGCACTTATTTTGAAAGAGATAGGGCAAGAAACACAAGGCAAAACAGTAGATCTAGAGACGACAAAACCCGTGGTGCGCATTCTCTGAATTCTGTCTTGAGGCAGTATAGAGGTGCTGATGACTTGGATTTTCCAGGATCAGAGGCTACTTCTGGCTCCAAAAGATGGGGTAACATTTCTGATGTTACATTTGGACGACAGAATCAAAGGCAAAAGGGCCCTTTAGATAGTGGGTTTTTCAGTCGAAGATCTTTTAAGGAAATTGGCTGTAGTGATGAAATTCTAGGTGCACTGAGAAGTTTTGGCTTCCCTCGTCCATCGCATATTCAGGCGATGGCATATCGACCTGTCTTGGAGGGGAAAAGCTGCATTATTGGTGATCAAAGTGGATCTGGCAAGACATTGGCATATCTTTGCCCAGTAGTACAAAACCTGAGGAAGGAAGAAGTTGAGGGGCTCCATAGGTCTTCTCCTAGGAACCCACGAGTGGTAGTACTGACGCCTACAGCTGAGCTTGCTTCTCAGGTCCTTAACAACTGCCGGTCAATATCGAAATCGGGGGTACCTTTTAGGTCTATGGTTGCAACTGGTGGATTTCGACAGAAGACACAGTTAGAAAGTCTTGATCAAGAGCTTGATGTACTTATTGCAACACCTGGTCGTTTCTTGTATCTGCTTCAAGAAGGCTTTGTGCAATTAAATAACCTCAGATGTGTTGTGCTGGATGAAGTGGATATTTTATATGGTGAAGAAAGTTTTGAACAAGTGCTTCATCAATTGATTACTGTTGCACCACTGACCACGCAATATCTCTTTGTCACTGCCACTCTTCCTCTCGACATCTACAACAAGGTTGTTGAAACATTTCCGGACTGTGAACTAATCATGGGACCAGGCGTCCACCGAACAAGCTCTCGTCTTGAAGAGATTCTCGTGGACTGCAGTGGAGATGACAATGAGGAAAAGAATCCGGAGACGGCGTTCTCAAACAAGAAATCAGCACTTGTAAAGATAATCGAGGAATCTCCTGTCCGTAAAACAATTATTTTCTGCAACAAGATTGAGACATGCAGAAAGGTTGAGAATGCGCTGAGGCGGGTGGACAGGAAGGCTTCGCAGATCAAAGTGCTCCCCTTCCATGCTGCATTGGATCAACAACAGAGAATCGCCAACATCAAAGAGTTCCTGAACAAGCAGACGGCTGACTCGATGTTCCTGGTGTGCACAGATAGGGCGTCGCGAGGCATCGACTTTGCGAACGTGAACCACGTGGTGCTGTTCGACTACCCGCGCGACCCGAGTGAGTACGTGCGGCGCGTCGGCAGGACGGCCCGCGGCGCGTCGGGCAACGGCAAGGCGTTCGTGTTCGCGGTGGGGAAGCAGGTGTCCCTCGCCAGGAGGGTGATGGAGAGGAACATCAAGGGCCACCCGCTGCACGATGTGCCATGCGTCTAGTCCTAGAAAGAGCTCTACAGAGCAGAGACAGTTTCAGGTCAAATTTGTGCTAGCATACATAGCACCATCTAATATTGATGTAGATAGGGTTTGTGAAACCGGTTTTGATGCATACACATATGCTGATCGAGTGAATTAGCATCTGTCTGTCTGTTTTAGAATGGTGATGCATATTGGTTCATACATACATTCATAGTGCTAGTAGTAGTAAATTTCAGTCGCTGACCAACTCACAAGTGGTGCAGCTGCTGCTGCTTGCTGTAAACGATGGAATGTGCAATTGGTTACTACACTACTAACTGAGATGAGACTGCTGCTGCTATTGACTTGGGTGGAGCTGGAGGGAGCAAGCAGGTCAGGTGTGGTCGTGGCGGACGGTGGCGAGGAGGTGGAGCTGGGCGTTGTGGAGGAGGGAGCGTGCCTGCTCGTTGGCGG
CDS Sequence
  • >LOC_Os03g01830.1
    ATGGAGGTGGCGGGTGCTCAGGCCGGCATCCTCCCCCTCCTCCTCCGTCACCCCGCGTCCCTCCGGGGCTCCCTCTCCCTCTCCTGCGGCGGCGCCCGCCGGAGCTGGGCGGCGGCGGCGGCCACGGCGGAAGGAGGAGGAGGAGAGGAGGGTCGCGGCTACGAGAGGGTGCCCATGGACACCCCCGGCGCCTACCGCCTCGTCGACCGGGCCACCGGCCGCAGCGTCATCGTGTGGGGCGGCACCGACGACGTCAGCATGCCCTCCCCCGCGGTGCTCTCCACGACCACGCGCGTCCCCGACCGCCCAAAAGAAAATGGGAGAAGTACAAGTATTGGAAATTTTGGCAGACTCAAGGCACAGAAAGTTAAAGTTTTAGCAAGGAGATCAGCTCACCTTAAGAGAGAGGATTCTGGTCGCATAAGCACCAGCCGGTTCAGTGAATCTCCTTCTGATGAGTCTGACGAAGATGGCACTTATTTTGAAAGAGATAGGGCAAGAAACACAAGGCAAAACAGTAGATCTAGAGACGACAAAACCCGTGGTGCGCATTCTCTGAATTCTGTCTTGAGGCAGTATAGAGGTGCTGATGACTTGGATTTTCCAGGATCAGAGGCTACTTCTGGCTCCAAAAGATGGGGTAACATTTCTGATGTTACATTTGGACGACAGAATCAAAGGCAAAAGGGCCCTTTAGATAGTGGGTTTTTCAGTCGAAGATCTTTTAAGGAAATTGGCTGTAGTGATGAAATTCTAGGTGCACTGAGAAGTTTTGGCTTCCCTCGTCCATCGCATATTCAGGCGATGGCATATCGACCTGTCTTGGAGGGGAAAAGCTGCATTATTGGTGATCAAAGTGGATCTGGCAAGACATTGGCATATCTTTGCCCAGTAGTACAAAACCTGAGGAAGGAAGAAGTTGAGGGGCTCCATAGGTCTTCTCCTAGGAACCCACGAGTGGTAGTACTGACGCCTACAGCTGAGCTTGCTTCTCAGGTCCTTAACAACTGCCGGTCAATATCGAAATCGGGGGTACCTTTTAGGTCTATGGTTGCAACTGGTGGATTTCGACAGAAGACACAGTTAGAAAGTCTTGATCAAGAGCTTGATGTACTTATTGCAACACCTGGTCGTTTCTTGTATCTGCTTCAAGAAGGCTTTGTGCAATTAAATAACCTCAGATGTGTTGTGCTGGATGAAGTGGATATTTTATATGGTGAAGAAAGTTTTGAACAAGTGCTTCATCAATTGATTACTGTTGCACCACTGACCACGCAATATCTCTTTGTCACTGCCACTCTTCCTCTCGACATCTACAACAAGGTTGTTGAAACATTTCCGGACTGTGAACTAATCATGGGACCAGGCGTCCACCGAACAAGCTCTCGTCTTGAAGAGATTCTCGTGGACTGCAGTGGAGATGACAATGAGGAAAAGAATCCGGAGACGGCGTTCTCAAACAAGAAATCAGCACTTGTAAAGATAATCGAGGAATCTCCTGTCCGTAAAACAATTATTTTCTGCAACAAGATTGAGACATGCAGAAAGGTTGAGAATGCGCTGAGGCGGGTGGACAGGAAGGCTTCGCAGATCAAAGTGCTCCCCTTCCATGCTGCATTGGATCAACAACAGAGAATCGCCAACATCAAAGAGTTCCTGAACAAGCAGACGGCTGACTCGATGTTCCTGGTGTGCACAGATAGGGCGTCGCGAGGCATCGACTTTGCGAACGTGAACCACGTGGTGCTGTTCGACTACCCGCGCGACCCGAGTGAGTACGTGCGGCGCGTCGGCAGGACGGCCCGCGGCGCGTCGGGCAACGGCAAGGCGTTCGTGTTCGCGGTGGGGAAGCAGGTGTCCCTCGCCAGGAGGGTGATGGAGAGGAACATCAAGGGCCACCCGCTGCACGATGTGCCATGCGTCTAG
Protein Sequence
  • >LOC_Os03g01830.1
    MEVAGAQAGILPLLLRHPASLRGSLSLSCGGARRSWAAAAATAEGGGGEEGRGYERVPMDTPGAYRLVDRATGRSVIVWGGTDDVSMPSPAVLSTTTRVPDRPKENGRSTSIGNFGRLKAQKVKVLARRSAHLKREDSGRISTSRFSESPSDESDEDGTYFERDRARNTRQNSRSRDDKTRGAHSLNSVLRQYRGADDLDFPGSEATSGSKRWGNISDVTFGRQNQRQKGPLDSGFFSRRSFKEIGCSDEILGALRSFGFPRPSHIQAMAYRPVLEGKSCIIGDQSGSGKTLAYLCPVVQNLRKEEVEGLHRSSPRNPRVVVLTPTAELASQVLNNCRSISKSGVPFRSMVATGGFRQKTQLESLDQELDVLIATPGRFLYLLQEGFVQLNNLRCVVLDEVDILYGEESFEQVLHQLITVAPLTTQYLFVTATLPLDIYNKVVETFPDCELIMGPGVHRTSSRLEEILVDCSGDDNEEKNPETAFSNKKSALVKIIEESPVRKTIIFCNKIETCRKVENALRRVDRKASQIKVLPFHAALDQQQRIANIKEFLNKQTADSMFLVCTDRASRGIDFANVNHVVLFDYPRDPSEYVRRVGRTARGASGNGKAFVFAVGKQVSLARRVMERNIKGHPLHDVPCV*