Information report for OsBIRH1;TCD33
Gene Details
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Functional Descriptions
- Disease resistance phenotype assays revealed that the OsBIRH1-overexpressing transgenic plants showed an enhanced disease resistance against Alternaria brassicicola and Pseudomonas syringae pv.
- Expression of OsBIRH1 was activated in rice seedling leaves after treatment with defence-related signal chemicals, for example benzothiadiazole, salicylic acid, l-aminocyclopropane-1-carboxylic acid, and jasmonic acid, and was also up-regulated in an incompatible interaction between a resistant rice genotype and the blast fungus, Magnaporthe grisea.
- The results suggest that OsBIRH1 encodes a functional DEAD-box RNA helicase and plays important roles in defence responses against biotic and abiotic stresses.
- Moreover, the OsBIRH1 transgenic Arabidopsis plants also showed increased tolerance to oxidative stress and elevated expression levels of oxidative defence genes, AtApx1, AtApx2, and AtFSD1.
- OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress.
- The TCD33 seedlings also exhibited less chlorophyll contents and severe defects of chloroplast structure under 20<U+2009>
C condition. - A DEAD-box RNA helicase TCD33 that confers chloroplast development in rice at seedling stage under cold stress.
- The transcript expression level of TCD33 indicated that the genes related to chlorophyll (Chl) biosynthesis, photosynthesis, and chloroplast development in TCD33 mutants were down-regulated at 20<U+2009>
C, while the down-regulated genes were nearly recovered to or slightly higher than the WT level at 32<U+2009> C. - Together, our results suggest that the cold-inducible TCD33 is essential for early chloroplast development and is important for cold-responsive gene regulation and cold tolerance in rice.
- Map-based cloning and complementation experiments suggested that TCD33 encodes a chloroplast-located DEAD-box RNA helicase protein.
Functional Keywords
- disease , jasmonic , abiotic-stress , salicylic-acid , seedling , jasmonic-acid , disease-resistance , blast , oxidative , biotic-stress , resistant , seedlings , chloroplast , development , map-based-cloning , tolerance , cold-tolerance , cold-stress , chloroplast-development , chlorophyll-content , cold , helicase
Literature and News
- OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress . DOI: 10.1093/jxb/ern072 ; PMID: 18441339
- A DEAD-box RNA helicase TCD33 that confers chloroplast development in rice at seedling stage under cold stress . DOI: 10.1016/j.jplph.2020.153138 ; PMID: 32213379
Gene Resources
- UniProt: Q0DVX2
- EMBL: AC113930, AK065776, AP008209
- AlphaFoldDB: Q0DVX2
- EnsemblPlants: Os03t0108600-01
- Gramene: Os03t0108600-01
- KEGG: dosa:Os03g0108600
- Orthologous matrix: GPANNDP
- InterPro: IPR001650, IPR011545, IPR014001
Sequences
cDNA Sequence
- >LOC_Os03g01830.1
ATCGGATAGGAAGGAAGGAGACGGCGGCGCTGCGAGAGATGGAGGTGGCGGGTGCTCAGGCCGGCATCCTCCCCCTCCTCCTCCGTCACCCCGCGTCCCTCCGGGGCTCCCTCTCCCTCTCCTGCGGCGGCGCCCGCCGGAGCTGGGCGGCGGCGGCGGCCACGGCGGAAGGAGGAGGAGGAGAGGAGGGTCGCGGCTACGAGAGGGTGCCCATGGACACCCCCGGCGCCTACCGCCTCGTCGACCGGGCCACCGGCCGCAGCGTCATCGTGTGGGGCGGCACCGACGACGTCAGCATGCCCTCCCCCGCGGTGCTCTCCACGACCACGCGCGTCCCCGACCGCCCAAAAGAAAATGGGAGAAGTACAAGTATTGGAAATTTTGGCAGACTCAAGGCACAGAAAGTTAAAGTTTTAGCAAGGAGATCAGCTCACCTTAAGAGAGAGGATTCTGGTCGCATAAGCACCAGCCGGTTCAGTGAATCTCCTTCTGATGAGTCTGACGAAGATGGCACTTATTTTGAAAGAGATAGGGCAAGAAACACAAGGCAAAACAGTAGATCTAGAGACGACAAAACCCGTGGTGCGCATTCTCTGAATTCTGTCTTGAGGCAGTATAGAGGTGCTGATGACTTGGATTTTCCAGGATCAGAGGCTACTTCTGGCTCCAAAAGATGGGGTAACATTTCTGATGTTACATTTGGACGACAGAATCAAAGGCAAAAGGGCCCTTTAGATAGTGGGTTTTTCAGTCGAAGATCTTTTAAGGAAATTGGCTGTAGTGATGAAATTCTAGGTGCACTGAGAAGTTTTGGCTTCCCTCGTCCATCGCATATTCAGGCGATGGCATATCGACCTGTCTTGGAGGGGAAAAGCTGCATTATTGGTGATCAAAGTGGATCTGGCAAGACATTGGCATATCTTTGCCCAGTAGTACAAAACCTGAGGAAGGAAGAAGTTGAGGGGCTCCATAGGTCTTCTCCTAGGAACCCACGAGTGGTAGTACTGACGCCTACAGCTGAGCTTGCTTCTCAGGTCCTTAACAACTGCCGGTCAATATCGAAATCGGGGGTACCTTTTAGGTCTATGGTTGCAACTGGTGGATTTCGACAGAAGACACAGTTAGAAAGTCTTGATCAAGAGCTTGATGTACTTATTGCAACACCTGGTCGTTTCTTGTATCTGCTTCAAGAAGGCTTTGTGCAATTAAATAACCTCAGATGTGTTGTGCTGGATGAAGTGGATATTTTATATGGTGAAGAAAGTTTTGAACAAGTGCTTCATCAATTGATTACTGTTGCACCACTGACCACGCAATATCTCTTTGTCACTGCCACTCTTCCTCTCGACATCTACAACAAGGTTGTTGAAACATTTCCGGACTGTGAACTAATCATGGGACCAGGCGTCCACCGAACAAGCTCTCGTCTTGAAGAGATTCTCGTGGACTGCAGTGGAGATGACAATGAGGAAAAGAATCCGGAGACGGCGTTCTCAAACAAGAAATCAGCACTTGTAAAGATAATCGAGGAATCTCCTGTCCGTAAAACAATTATTTTCTGCAACAAGATTGAGACATGCAGAAAGGTTGAGAATGCGCTGAGGCGGGTGGACAGGAAGGCTTCGCAGATCAAAGTGCTCCCCTTCCATGCTGCATTGGATCAACAACAGAGAATCGCCAACATCAAAGAGTTCCTGAACAAGCAGACGGCTGACTCGATGTTCCTGGTGTGCACAGATAGGGCGTCGCGAGGCATCGACTTTGCGAACGTGAACCACGTGGTGCTGTTCGACTACCCGCGCGACCCGAGTGAGTACGTGCGGCGCGTCGGCAGGACGGCCCGCGGCGCGTCGGGCAACGGCAAGGCGTTCGTGTTCGCGGTGGGGAAGCAGGTGTCCCTCGCCAGGAGGGTGATGGAGAGGAACATCAAGGGCCACCCGCTGCACGATGTGCCATGCGTCTAGTCCTAGAAAGAGCTCTACAGAGCAGAGACAGTTTCAGGTCAAATTTGTGCTAGCATACATAGCACCATCTAATATTGATGTAGATAGGGTTTGTGAAACCGGTTTTGATGCATACACATATGCTGATCGAGTGAATTAGCATCTGTCTGTCTGTTTTAGAATGGTGATGCATATTGGTTCATACATACATTCATAGTGCTAGTAGTAGTAAATTTCAGTCGCTGACCAACTCACAAGTGGTGCAGCTGCTGCTGCTTGCTGTAAACGATGGAATGTGCAATTGGTTACTACACTACTAACTGAGATGAGACTGCTGCTGCTATTGACTTGGGTGGAGCTGGAGGGAGCAAGCAGGTCAGGTGTGGTCGTGGCGGACGGTGGCGAGGAGGTGGAGCTGGGCGTTGTGGAGGAGGGAGCGTGCCTGCTCGTTGGCGG
CDS Sequence
- >LOC_Os03g01830.1
ATGGAGGTGGCGGGTGCTCAGGCCGGCATCCTCCCCCTCCTCCTCCGTCACCCCGCGTCCCTCCGGGGCTCCCTCTCCCTCTCCTGCGGCGGCGCCCGCCGGAGCTGGGCGGCGGCGGCGGCCACGGCGGAAGGAGGAGGAGGAGAGGAGGGTCGCGGCTACGAGAGGGTGCCCATGGACACCCCCGGCGCCTACCGCCTCGTCGACCGGGCCACCGGCCGCAGCGTCATCGTGTGGGGCGGCACCGACGACGTCAGCATGCCCTCCCCCGCGGTGCTCTCCACGACCACGCGCGTCCCCGACCGCCCAAAAGAAAATGGGAGAAGTACAAGTATTGGAAATTTTGGCAGACTCAAGGCACAGAAAGTTAAAGTTTTAGCAAGGAGATCAGCTCACCTTAAGAGAGAGGATTCTGGTCGCATAAGCACCAGCCGGTTCAGTGAATCTCCTTCTGATGAGTCTGACGAAGATGGCACTTATTTTGAAAGAGATAGGGCAAGAAACACAAGGCAAAACAGTAGATCTAGAGACGACAAAACCCGTGGTGCGCATTCTCTGAATTCTGTCTTGAGGCAGTATAGAGGTGCTGATGACTTGGATTTTCCAGGATCAGAGGCTACTTCTGGCTCCAAAAGATGGGGTAACATTTCTGATGTTACATTTGGACGACAGAATCAAAGGCAAAAGGGCCCTTTAGATAGTGGGTTTTTCAGTCGAAGATCTTTTAAGGAAATTGGCTGTAGTGATGAAATTCTAGGTGCACTGAGAAGTTTTGGCTTCCCTCGTCCATCGCATATTCAGGCGATGGCATATCGACCTGTCTTGGAGGGGAAAAGCTGCATTATTGGTGATCAAAGTGGATCTGGCAAGACATTGGCATATCTTTGCCCAGTAGTACAAAACCTGAGGAAGGAAGAAGTTGAGGGGCTCCATAGGTCTTCTCCTAGGAACCCACGAGTGGTAGTACTGACGCCTACAGCTGAGCTTGCTTCTCAGGTCCTTAACAACTGCCGGTCAATATCGAAATCGGGGGTACCTTTTAGGTCTATGGTTGCAACTGGTGGATTTCGACAGAAGACACAGTTAGAAAGTCTTGATCAAGAGCTTGATGTACTTATTGCAACACCTGGTCGTTTCTTGTATCTGCTTCAAGAAGGCTTTGTGCAATTAAATAACCTCAGATGTGTTGTGCTGGATGAAGTGGATATTTTATATGGTGAAGAAAGTTTTGAACAAGTGCTTCATCAATTGATTACTGTTGCACCACTGACCACGCAATATCTCTTTGTCACTGCCACTCTTCCTCTCGACATCTACAACAAGGTTGTTGAAACATTTCCGGACTGTGAACTAATCATGGGACCAGGCGTCCACCGAACAAGCTCTCGTCTTGAAGAGATTCTCGTGGACTGCAGTGGAGATGACAATGAGGAAAAGAATCCGGAGACGGCGTTCTCAAACAAGAAATCAGCACTTGTAAAGATAATCGAGGAATCTCCTGTCCGTAAAACAATTATTTTCTGCAACAAGATTGAGACATGCAGAAAGGTTGAGAATGCGCTGAGGCGGGTGGACAGGAAGGCTTCGCAGATCAAAGTGCTCCCCTTCCATGCTGCATTGGATCAACAACAGAGAATCGCCAACATCAAAGAGTTCCTGAACAAGCAGACGGCTGACTCGATGTTCCTGGTGTGCACAGATAGGGCGTCGCGAGGCATCGACTTTGCGAACGTGAACCACGTGGTGCTGTTCGACTACCCGCGCGACCCGAGTGAGTACGTGCGGCGCGTCGGCAGGACGGCCCGCGGCGCGTCGGGCAACGGCAAGGCGTTCGTGTTCGCGGTGGGGAAGCAGGTGTCCCTCGCCAGGAGGGTGATGGAGAGGAACATCAAGGGCCACCCGCTGCACGATGTGCCATGCGTCTAG
Protein Sequence
- >LOC_Os03g01830.1
MEVAGAQAGILPLLLRHPASLRGSLSLSCGGARRSWAAAAATAEGGGGEEGRGYERVPMDTPGAYRLVDRATGRSVIVWGGTDDVSMPSPAVLSTTTRVPDRPKENGRSTSIGNFGRLKAQKVKVLARRSAHLKREDSGRISTSRFSESPSDESDEDGTYFERDRARNTRQNSRSRDDKTRGAHSLNSVLRQYRGADDLDFPGSEATSGSKRWGNISDVTFGRQNQRQKGPLDSGFFSRRSFKEIGCSDEILGALRSFGFPRPSHIQAMAYRPVLEGKSCIIGDQSGSGKTLAYLCPVVQNLRKEEVEGLHRSSPRNPRVVVLTPTAELASQVLNNCRSISKSGVPFRSMVATGGFRQKTQLESLDQELDVLIATPGRFLYLLQEGFVQLNNLRCVVLDEVDILYGEESFEQVLHQLITVAPLTTQYLFVTATLPLDIYNKVVETFPDCELIMGPGVHRTSSRLEEILVDCSGDDNEEKNPETAFSNKKSALVKIIEESPVRKTIIFCNKIETCRKVENALRRVDRKASQIKVLPFHAALDQQQRIANIKEFLNKQTADSMFLVCTDRASRGIDFANVNHVVLFDYPRDPSEYVRRVGRTARGASGNGKAFVFAVGKQVSLARRVMERNIKGHPLHDVPCV*