Information report for Lsi2;OsLsi2
Gene Details
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Functional Descriptions
- Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots.
- Furthermore, in the rice mutants defective in the silicon efflux transporter Lsi2, arsenite transport to the xylem and accumulation in shoots and grain decreased greatly.
- Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier).
- The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath.
- Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter.
- By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium.
- Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1.
- Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1.
- There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions.
- A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si.
- The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice.
- Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice.
- In summary, our results reveal that Si deposition at root endodermis and its resultant reduction of Na+ bypass flow is modulated by OsLsi1 and OsLsi2 and regulated by the expression of OsLsi1 and OsLsi2, implying that root Si deposition could be an active and physiologically-regulated process in rice.
- In this study, we investigated the roles of OsLsi1 and OsLsi2 in Si-induced reduction of bypass flow and its resultant alleviation of salt stress by using lsi1 and lsi2 mutants (defective in OsLsi1 and OsLsi2, respectively) and their wild types (WTs).
- The polar localization of both OsLsi1 and OsLsi2 was not altered by Si supply, but their protein abundance was reduced.
- The polar localization of OsLsi1 and OsLsi2 was not altered by inhibition of clathrin-mediated endocytosis (CME) by dominant-negative induction of dynamin-related protein1A and knockout of mu subunit of adaptor protein 2 complex, although the knockout mutants of OsAP2M gene showed dwarf phenotype.
Functional Keywords
Literature and News
- The role of the rice aquaporin Lsi1 in arsenite efflux from roots . DOI: 10.1111/j.1469-8137.2010.03192.x ; PMID: 20163552
- An efflux transporter of silicon in rice . DOI: 10.1038/nature05964 ; PMID: 1762556
- Genotypic difference in silicon uptake and expression of silicon transporter genes in rice . DOI: 10.1104/pp.107.107599 ; PMID: 17905867
- The rice aquaporin Lsi1 mediates uptake of methylated arsenic species . DOI: 10.1104/pp.109.140350 ; PMID: 19542298
- Transporters of arsenite in rice and their role in arsenic accumulation in rice grain . DOI: 10.1073/pnas.0802361105 ; PMID: 18626020
- High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice . DOI: 10.1093/pcp/pcw163 ; PMID: 27742884
- Altered Root Structure Affects Both Expression and Cellular Localization of Transporters for Mineral Element Uptake in Rice . DOI: 10.1093/pcp/pcz213 ; PMID: 31747007
- Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice . DOI: 10.1016/j.plaphy.2020.11.015 ; PMID: 33243712
- Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice . DOI: 10.1093/pcp/pcac032 ; PMID: 35277719
Gene Resources
- UniProt: Q10SY9
- EMBL: AB222273, AC113930, AK101092
- AlphaFoldDB: Q10SY9
- EnsemblPlants: Os03t0107300-01
- Gramene: Os03t0107300-01
- KEGG: dosa:Os03g0107300
- Orthologous matrix: IAMFIDV
Sequences
cDNA Sequence
- >LOC_Os03g01700.1
AGCATCAGCAGCGCATGCACTAGCAGCTTAGCTACTGCGCGCGTACCAGAGAGAGATCATCAGCTCAAGTAGCTAAGCTAGCTATAATCTGCTAGCTAGCTCGATCAGACACTTAATTACCTGCTAGGTGGTGGTCGATCGAAGAAGAAGAAGATGAGTGAGCTTGCGTCGGCGCCCAAGGTGGCGCTTGGATCGATCGCGTTCGCGGTGTTCTGGATGATGGCGGTGTTCCCGTCGGTGCCGTTCCTGCCGATCGGGCGGACGGCGGGGTCGCTGCTGAGCGCGGTGCTGATGGTGATATTCCACGTGATCAGCCCCGACGACGCGTACGCCTCCATCGACCTCCCAATCCTGGGCCTCCTCTTCGCCACCATGGTGGTGGGCAGCTACCTCCGGAACGCCGGGATGTTCAAGCACCTGGGGCGTCTGCTGGCGTGGAAGAGCCAGGGCGGGCGCGACCTCATGTGCCGCGTCTGCGTCGTCACCGCCCTCGCCAGCGCCCTCTTCACCAACGACACCTGCTGCGTCGTCCTCACCGAGTTCGTCCTCGAGCTCGCCGCCGAGCGCAACCTCCCCGCCAAGCCCTTCCTCCTCGCCCTCGCCTCCAGCGCCAACATCGGCTCCGCCGCCACCCCCATCGGCAACCCCCAGAACCTGGTCATCGCCTTCAATAGCAAGATCACCTTCCCCAAGTTCCTCATGGGAATCCTCCCGGCCATGCTCGTCGGGATGGCCGTCAACATGGTCATGCTGCTCTGCATGTACTGGAGGGAGCTGGGCGGAGGGGCCGAGCTCAGCGTCGACGGCAAGCAGATGGAGGCGGTGGAGGAAGGCAGGTCGCCGGCGTCGGCCAAGAGCACGCCGCAGCTGAACGGCAACGGCAACACGATGATGTCGCTGGAGATGTCGGAGAACATAACGACCAAGCACCCATGGTTCATGCAGTGCACGGAGGCGCGGCGGAAGCTGTTCCTCAAGAGCTTCGCGTACGTGGTGACGGTGGGGATGGTGGTGGCCTACATGGTGGGGCTCAACATGTCGTGGACGGCCATCACCACGGCGCTGGCGCTGGTGGTGGTCGACTTCCGCGACGCCGAGCCGTGCCTGGACACCGTGTCCTACTCGCTGCTCGTCTTCTTCTCCGGGATGTTCATCACCGTCAGCGGCTTCAACAAGACGGGCCTCCCGGGAGCCATCTGGGACTTCATGGCCCCCTACTCCAAGGTCAACAGCGTCGGCGGCATCTCCGTCCTCTCCGTCATCATCCTCCTCCTCTCCAACCTCGCATCAAACGTACCAACGGTGCTTCTTATGGGTGATGAGGTGGCGAAGGCGGCGGCGCTGATATCGCCGGCGGCGGTGACGACGTCGTGGCTGCTGCTGGCGTGGGTGAGCACGGTGGCGGGGAACCTGTCGCTGCTGGGGTCGGCGGCGAACCTGATAGTGTGCGAGCAGGCGAGGAGGGCGCCCAGGAACGCCTACGACCTCACCTTCTGGCAGCACATCGTCTTCGGCGTCCCATCCACCCTCATCGTCACCGCCGTCGGCATACCCCTCATCGGCAAGATCTGATCTCATCTCATCGACCCATCCAAATTAATTAATTATGAGATCGACAAACATCCAAGCTTGCTAGGCTCGTCGTCGTCGTCGTCGACCACCGTACCATATATATGCATGCATGCCACGCACGTATATATATGCTCCTCAACCTCAAGTCAATTAATTAAGAGAATGGATGAATGAATCAATGTTGTGTATGATTTCCTTTTTGTTTTTGTTTTACCCACCGTATATGTGCTGTGTGGTGTATGGCCGGCGAGCTTGAAATTGAATTGGCCTTGCTTGCATGCATGCATGGTGATCAGATCAGCTCAGCTAGCTAGCTAAATCTTGCTTAATTTTATCAAGTGTCAATCATGATGAGAAGAGAGGGAACTAGATGGAGCTAGCTAGCTAGCAACACATGCAGTGTGTGCTAAGCAGTGCACTCTGCATGCTTAAATTTGCTTGCTTAATTCAACATCGTATTACATGTATGTTGATATGTGCATGTTCCTCAAAATACATGGAAAAAAAATGTTTCTACACATCTCAATCGTATAGAGTATGTAGCATGTCTTGTTCAAACAAATATTTGTAGCCCTCTCTCTCTCTCTCTCTTTAGGTTTATTAATTAATTAGTCTGTTCAATGTCACCTCAGTTCATAGAGATGCTATACTTAATTAATTGCTCCATGTATGCAGAGTTGTACTACCTTAAAATATCAGTATGCATGCTCTTGTTGCCATTCCAAAAGAACAAA
CDS Sequence
- >LOC_Os03g01700.1
ATGAGTGAGCTTGCGTCGGCGCCCAAGGTGGCGCTTGGATCGATCGCGTTCGCGGTGTTCTGGATGATGGCGGTGTTCCCGTCGGTGCCGTTCCTGCCGATCGGGCGGACGGCGGGGTCGCTGCTGAGCGCGGTGCTGATGGTGATATTCCACGTGATCAGCCCCGACGACGCGTACGCCTCCATCGACCTCCCAATCCTGGGCCTCCTCTTCGCCACCATGGTGGTGGGCAGCTACCTCCGGAACGCCGGGATGTTCAAGCACCTGGGGCGTCTGCTGGCGTGGAAGAGCCAGGGCGGGCGCGACCTCATGTGCCGCGTCTGCGTCGTCACCGCCCTCGCCAGCGCCCTCTTCACCAACGACACCTGCTGCGTCGTCCTCACCGAGTTCGTCCTCGAGCTCGCCGCCGAGCGCAACCTCCCCGCCAAGCCCTTCCTCCTCGCCCTCGCCTCCAGCGCCAACATCGGCTCCGCCGCCACCCCCATCGGCAACCCCCAGAACCTGGTCATCGCCTTCAATAGCAAGATCACCTTCCCCAAGTTCCTCATGGGAATCCTCCCGGCCATGCTCGTCGGGATGGCCGTCAACATGGTCATGCTGCTCTGCATGTACTGGAGGGAGCTGGGCGGAGGGGCCGAGCTCAGCGTCGACGGCAAGCAGATGGAGGCGGTGGAGGAAGGCAGGTCGCCGGCGTCGGCCAAGAGCACGCCGCAGCTGAACGGCAACGGCAACACGATGATGTCGCTGGAGATGTCGGAGAACATAACGACCAAGCACCCATGGTTCATGCAGTGCACGGAGGCGCGGCGGAAGCTGTTCCTCAAGAGCTTCGCGTACGTGGTGACGGTGGGGATGGTGGTGGCCTACATGGTGGGGCTCAACATGTCGTGGACGGCCATCACCACGGCGCTGGCGCTGGTGGTGGTCGACTTCCGCGACGCCGAGCCGTGCCTGGACACCGTGTCCTACTCGCTGCTCGTCTTCTTCTCCGGGATGTTCATCACCGTCAGCGGCTTCAACAAGACGGGCCTCCCGGGAGCCATCTGGGACTTCATGGCCCCCTACTCCAAGGTCAACAGCGTCGGCGGCATCTCCGTCCTCTCCGTCATCATCCTCCTCCTCTCCAACCTCGCATCAAACGTACCAACGGTGCTTCTTATGGGTGATGAGGTGGCGAAGGCGGCGGCGCTGATATCGCCGGCGGCGGTGACGACGTCGTGGCTGCTGCTGGCGTGGGTGAGCACGGTGGCGGGGAACCTGTCGCTGCTGGGGTCGGCGGCGAACCTGATAGTGTGCGAGCAGGCGAGGAGGGCGCCCAGGAACGCCTACGACCTCACCTTCTGGCAGCACATCGTCTTCGGCGTCCCATCCACCCTCATCGTCACCGCCGTCGGCATACCCCTCATCGGCAAGATCTGA
Protein Sequence
- >LOC_Os03g01700.1
MSELASAPKVALGSIAFAVFWMMAVFPSVPFLPIGRTAGSLLSAVLMVIFHVISPDDAYASIDLPILGLLFATMVVGSYLRNAGMFKHLGRLLAWKSQGGRDLMCRVCVVTALASALFTNDTCCVVLTEFVLELAAERNLPAKPFLLALASSANIGSAATPIGNPQNLVIAFNSKITFPKFLMGILPAMLVGMAVNMVMLLCMYWRELGGGAELSVDGKQMEAVEEGRSPASAKSTPQLNGNGNTMMSLEMSENITTKHPWFMQCTEARRKLFLKSFAYVVTVGMVVAYMVGLNMSWTAITTALALVVVDFRDAEPCLDTVSYSLLVFFSGMFITVSGFNKTGLPGAIWDFMAPYSKVNSVGGISVLSVIILLLSNLASNVPTVLLMGDEVAKAAALISPAAVTTSWLLLAWVSTVAGNLSLLGSAANLIVCEQARRAPRNAYDLTFWQHIVFGVPSTLIVTAVGIPLIGKI*